Kmt2e (NM_026984) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810033J14Rik; 9530077A04Rik; D230038D11Rik; Mll5; NKp44L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224990 representing NM_026984 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATAGCGATCCCATTGGGGGTTGATACAACAGAAACATCCTACTTGGAAATGGCTGCAGGCTCAG AACCGGAATCCGTAGAAGCTAGCCCTGTGGTAGTTGAGAAATCCAACAGTTTTCCCCACCAGTTATACAC CAGCAGCTCCCACCACTCACACAGCTACATCGGTCTGCCCTATGCGGACCATAATTATGGTGCTCGTCCT CCTCCAACACCTCCAGCATCCCCTCCTCCATCAGGTCTCATTAGCAAAAACGAAGTAGGCATATTTACCA CTCCTAATTTTGATGAAACTTCCAGTGCTACTACAATCAGCACATCTGAAGATGGAAGTTACGGTACTGA TGTAACCAGGTGCATATGTGGTTTTACACATGATGACGGATACATGATCTGTTGTGACAAATGCAGTGTT TGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTC AGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGG TGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCA ACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGA AAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGG TTCAGCACCAGAAATTGATCCTTCATCTGATAGTTCAAATTTTGTTTGGGAAACAAAAATCAAAGCATGG ATGGATCGTTACGAAGAAGCAAATAATAACCAATATAGTGAAGGTGTTCAGAGAGAGGCACAAAGACTAG CTCAAAGACTCGGTAGTGGGAATGACAGCAAAGACATGAATAAATCAGAATTGAGTACCAACAACTCACT CTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCT CCTGATGCACTTATCATTGAATATAGAGGGAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATT TCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCTAAGTTCCATGGGCTTGAAATGTGTGTTGATGC GAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAA ATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCA TTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGA GTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAA AAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTG AAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGT GTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAA ATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAA TTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCAC AGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAA CAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCT TTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACAT ACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATA GAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTC CCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGG AAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAG CTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTA CATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAA GCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAG GAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCC TCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAG CTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTT GCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAG TGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTC TCCAGAAATAAAGAGATGCACTTACAATCAAGAGGGATATGACCGACCTTCAAACATGCTAACGTTGGGG CCTTTTAGAAATTCCAATTTAACTGAGCTGGGCCTGCAAGAAATAAAGACTATTGGTTATACCAGCCCTA GGAGCAGGACTGAGGTTAACAGGCCTTGCCCAGGGGAGAAGGAGTCTGTGTCAGACCTTCAACTGGGACT CGATGCAGTCGAGCCAGCTGCCTTACAGAAATCCATGGAAACACCAGCCCATGACAGGACTGAGCCCAGC AACCAACTGGATTCGACCCACTCTGGACGGGGCACAATGTATTCATCCTGGGTAAAGAGTCCTGACAGAA CAGGAGTTAACTTCTCAGTAAACTCCAACTTGAGGGACTTAACACCCTCTCATCAGTTGGAGACTGGAGG CGGCTTCCGAGTAAGTGAGTCCAAGTGCCTGATTCAGCAGGATGACACTAGAGGCATGTTTCTGGGAGCG GCTGTCTTTTGTACTTCTGAAGACGGGCTTGCCTCTGGTTTTGGACGGACTGTTAATGACAATTTGATCG ACGGGAGTTGCACGCCCCAGAATCCACCACAAAAGAAAAAGGTTTCTCTATTAGAATACCGTAAGAGACA ACGTGAAGCTAGGAAGAGTGGGTCTAAGCCAGAGAACTTTGCTTTGATTAGTGTGTCACCTCACCCGAGT GGGAGCCTGAGCAGCAGTGGCGACGGGTGTGTCCACAGCAGTGAAAACGGGGAGCAGGCAGAAAACCAGG CTAGCTTGCCTTTACCACCGCCTGCTGCCGCCGCCGCCGCCACCGCCGCCGCCGCTTACAGTGCCTCTTC TGAAGAAGGCAGCAGTAACTGTCCTGTTAAAGACGCCAATTCCAGTGAGAAGAAAGACCCAGAAGTTCAG TGGACTGCCTCCACTTCAGTGGAGCAAGTGAGAGAGAGAAGTTACCAGAGAGCCTTACTGCTCAGTGACC ACCGCAAAGACAAAGACAGTGGGGGAGAGTCACCATGTGTCTCATGCTCACCGAGTCATGTTCAGTCTCC ACCTTCATCTCATTCAAATCACATTCCCCAGGTGCACGCTCAGAGCCTAGCTCCTTCTTTGAGTGAACTC ATGGCAGACCCTGATGCGGAAGGTACAGAAGCCACAAGTACAAGTGAATGTCCATCCCCTGACACTTCTC AGAGCCCTTCTAAAACAAGCAAGCCTGGTTCACCAGGGCCAATCAATCCTGCTCAGTCACATGGGAAAAT ACTCACAAAACCAGATAGCCACTGGGAGGCAACTGCCACTGTATCAGAAGCTGACAACAGTGTTCACCAA AACCCAGAGCCTCAGCACAGACAGCTGTCAAGTAACACCCCAGCACTTTCTCAGAACCATGCTCCTCAGG CCCACGCTCTGAGTGCGAATGATCAACTTCCGCAAAAGCTGCCTTCTGCACCGACGAAGTTGCACTGTCC TCCGTCACCTCACACAGAAAACCCTCCCAAGTCCTCCACACCTCACACCCCCGTGCAGCATGGTTACCTC TCGCCAAAGCCTCCTTCACAGCACTTAGGATCTCCCTTCAGGCCTCACCATTCACAATCACCTCAAGTTG GAACTCCTCAACGAGAGACTCAGAGAAACTTTTATGCAGCGGCACAGAACCTTCAAGCTAATCCTCAGCA GGCAACTTCTGGAGCGTTATTTACCCAGACACCCTCAGGACAATCTTCAGCAACCTACAGTCAGTTCAAC CAACAGAGTCTCAACAGCACGGCCCCACCCCCTCCCCCTCCTCCCCCTCCTTCATCCTACTATCAAAACC AGCAGCCCTCTGCAAACTTTCAGAATTATAATCAGCTAAAAGGTAGTCTTTCCCAACAAACTGTGTTTAC ATCTGGACCAAATCAAGCACTTCCTGGCAGCACCAGCCAGCAATCAGTTCCTGGACATCACGTTACTCCA GGGCATTTTCTGCCATCTCAAAACCCCACCATTCACCATCAACCTGCTGCTGCTGCTGTGGTCCCGCCCC CGCCCCCACCCCCGCCCGCTCCAGGACCACACCTCATACAGCAGCCAAGCTCACATCAGCAGCACTCTGT AGCACATGGAGTTGGGCCAGTTCATGCTGTTACCCCTGGGTCACACATTCATTCTCAAACTGCAGGACAC CATTTACCACCACCCCCTCCACCACCTGGTCCTGCCCCACATCACCATCCCCCACCCCATCCTACCACGG GACTGCAAAGTCTACAAGCACAACACCAGCATGTAGTAAACTCGGCACCCCCGCCCCCTCCCCCTCCCCC GCCCCCTCCCCCAGCCAGTGTTCTGGTTTCTGGGCATCATTCAGCATCAGGTCAAGCCTTACACCACCCA CCTCATCAAGGACCTCCACTTTTCCCTGCAAGCGCTCATCCAGCTGTCCCGCCGTACCCCTCACAAGCCA CACATCATACCACTTTGGGACCGGGACCCCAACACCAGCCTTCCGGAACAGGGCCACATTGTCCATTACC AGTTGCAGGTCCTCATCTCCAGCCCCAAGGACCAAACAGTATTCCAACCCCTACTGCTTCAGGGTTCTGT CCTCATCCTCATCCTGGCTCTGTGGCCCTGCCACATGGGGTGCAAGGACCTCAGCAGGCATCGCCAGTGC CTGCACAGATTCCAATTCACAGAGCACAGGTGCCGCCGACATTTCAAAACAATTACCACGGTTCAGGGTG GCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_026984
Insert Size	5607 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_026984.1, NP_081260.1
RefSeq Size	7258 bp
RefSeq ORF	5607 bp
Locus ID	69188
UniProt ID	Q3UG20
Gene Summary	Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (By similarity). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (By similarity). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (PubMed:18854576, PubMed:18952892, PubMed:18818388). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:19264965). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (By similarity). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (PubMed:19264965).[UniProtKB/Swiss-Prot Function]

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