Pcdh15 (NM_001142737) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224987 representing NM_001142737 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACTATCGTGGCCATTGA TGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACTGCCGGAGGACCAGAC CCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACCCCGTTAAACAGATGC TTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACACTCCATTGTGGTGCA AGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATCGTGGTGCGAGATCGG AATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGCTCACTCCAGTTGGCA CCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGGCCCTAATGGACAGAT AGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAAATTCCACTCATGCTG ACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACTATGTCATCATCCAAG CAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCACAGTAGATGTTCTAGA TGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGTGACTGTCGTCCACTC ACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTTTGGTGACACCACCTA TCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCATCCTCTACTCCATCCT TGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCAGAACTCACTCTCCTG GAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGCAGGACAATGGCCACC CACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAGTCCATACTTCACAAT GCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCTGAGAGCCTAAACTTA ACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATCCAGAGCTCCACCTCT TCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTACCTCACCCTGCTTCA ACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGATGGCGTGCAAGAAAGT GAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCTTCCCTGAAATCTCCT ATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGACAGCGGTAGATGCTGA TGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGACTTCGTGATCAACAAG ACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGTATGCGCTCACAGTGC AGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGACCG CGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCGGATGGAACCTCAACTGCC ACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACC TCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAACAGACCCAGATGCTGGGAT AAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATCACATCCAACGGGAGCATT TACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTGTCGTGGCAACAGATGGAG CAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACATTGATGATAACAGTCCTGT TTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGGACCTCCTTTCTTCAAATA GAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCCCAGAAGTGAAACACCTTT TTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCGGACCA GGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATGCCACCTGGTATAGCAACA GTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAACGCATCTACAAGGGGATGG TGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGATGCGGACCCACCTGGGAT GCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCAGCCAGTATTTTTGATGTA GAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTACTACGATTTTCAAGCTGG TGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGTGAGAATTCTTGTCTTACA TCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTAAGTGAGCTTGCGGCCAGA GGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGTACTCCATTGTGGCAGGAA ATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAATTCACCATTGGATTACGA GACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTCCTTGCCAATCTCCGAGTC CCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCC AGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCAAGGATGTTCGCATCTGTGCTCAGAGTGAAGGC CACCGACAGGGACACGGGTAATTACAGTGCCATGGCCTACCGGCTCATCATACCGCCGATTAAAGAGGGC AAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAA GATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGACTACGGGAAGGGGTTGAGCGGGAAAGCAGACGT ACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCATTGTCTCCAATGTGCCCCCTACACTAGTGGAA AAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTACGTTCAGGAGCAAATTCCTGGTGCCAAGGTTG TGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCCTACTCCCTAGAAGACTATAGCAAGTGCGACCT GACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGAC GGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTATTACGGGGAAGGAGGGCGCATTCTGGAGATTC GGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGAGAAAGCTTGGGGTACACAGAAGGGGCCTTGCT GGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAGCCATCTTGGTCGTCTTAGTAAGCTACCGACAG TTTAAAGTACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTG CAGCTCCTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTA TGAAGAACTGGGAGAGAGCGCAATGCATAATCTTTTCCTTCTCTACCATTTTGAACAAAGCAGGGGAAAT AACTCAGTCCCAGAAGACAGGAGCAGTCATCGCGATGGGATGGCCTTTTCCTCCAGTACCACTGAGTCTC ATGAGCCAGCTCATGTAGAGGGACCACTTAAGGAGAGCCAGCCTAACCCAGCAAGGACGTTCTCATTTGT TCCTGATGAGGATAACTTAAGTACCCATAATCCCCTTTACATGGAAAGTATAGGTCAAAGGTCAACAAAC TCAGACCTTCAGCCACGAACAGATTTTGAAGAGCTGTTGGCACCCAGAACACAAGTTAAGAGTCAGTCTC TGAGGGGCCCAAGAGAAAAGATCCAGAGGGTGTGGAATCAGTCTGTGAGCTTTCCTAGGCGGCTCATGTG GAAAGCCCCAAACAGGCCAGAGACCATAGACCTGGTGGAGTGGCAGATCACCAATCAGAGAGCTGAATGC GAAAGCGCCAGATGCCACCCAAGCCAGAGAGGTAGCAGCAACGTTCTGCTGGCAACTGAAGATGCCCACG AGTCAGAGAAAGAAGGGGGACACAGAGACACCCTAATCGTCCAGCAAACAGAGCAGCTGAAATCTCTGTC TTCTGGCTCTTCTTTTTCCTCCTCTTGGTCTCACTTTTCTTTCTCAACTCTGCCAACGATTTCCAGAGCG GTGGAACTCGGGTCGGAACCTAATGTGGTCACTTCTCCCGCTGACTGCACCTTGGAACTTTCTCCTCCTC TGAGACCCCGTATTTTAAACTCCTTAAGCTCTAAGAGAGAGACTCCCACATGTGCATCAGATACAGAACC AAAAAGGAACTCTTTTGAGATCGCTCCCCATCCACCTAGCATCTCTGCTCCCCTCCCACATCCGCCTCTT CCTAGACCTCCCATTGCCTTTACCACTTTTCCTCTTCCCCTTTCTCCCCCTAACCCTCCTCCCCCACAAC TTGTTACATTTTCTCTTCCCATTTCTACACCCCCTACTTCTTCTCTACCTCTTCCTCCTCCACTGTCACT TCCTCCTCCTCCTCGGCCACCAGCTCCCCGCCTCTTCCCACAGCCTCCTTCCACGTCCATTCCATCCACA GACAGCATCTCTGCACCAGCTGCTAAATGCACTGCCAGTGCCACACACGCCAGAGAAACCACGTCTACGA CACAGCCACCAGCATCCAACCCGCAGTGGGGGGCAGAACCCCACAGACATCCAAAAGGGATCCTCAGACA TGTGAAAAACTTGGCAGAGCTCGAGAAATCAGTGTCTAACATGTACAGTCACATAGAAAAAAACTGCCCA CCTGCAGATCCCTCAAAACTACACACGTTTTGCCCTGCAGAGAAAACAGGCATGAAAATCACACATGACC AGAGCCAGGAAACGTTGGTTAGAGTTGTTGAGGGAATTGACGTGCAACCTCACAGTCAATCAACATCTTT GTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001142737
Insert Size	5604 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001142737.1, NP_001136209.1
RefSeq Size	8836 bp
RefSeq ORF	5604 bp
Locus ID	11994
UniProt ID	Q99PJ1
Gene Summary	Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (D) lacks an alternate in-frame exon in the 5' coding region and two alternate in-frame exons in the central coding region, compared to variant A. The resulting isoform (CD1-6), also known as protocadherin-15-CD1 isoform 6, lacks a 5-aa segment near the N-terminus and a 71-aa segment in the central protein, compared to isoform CD1-1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.