Afdn (NM_010806) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5033403D15Rik; Af-6; AF6; Afadin; Gm314; I-afadin; Mllt4; S-afadin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224949 representing NM_010806 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGCGGCCGCGACGAAGAGCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACC GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTT TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGAGTCTCTAGCACAGCCACCACTCAAGAT GTGATTGAAACACTGGCAGAGAAGTTCCGCCCTGACATGCGCATGCTCTCCTCTCCCAAGTACTCCCTCT ATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAAACCTCTAGTTGTACAGTTGAATTG GAACAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATGACGCCATTCCTGCCAAGAAGGCT CAGAGTAATGGACCTGAAAAGCAGGAGAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTCTCAAAGA AAGAAAAGAAGGAAAAAAAGAAGAAAGAGAAGGAGGCATTGAGACAAGCCTCTGATAAGGAAGAAAGGCC TTCTCAAGGGGATGACAGTGAGAACTCACGACTGGCTGCTGAAGTTTACAAAGACATGCCTGAGACCAGC TTTACTAGGACCATTTCTAACCCTGAAGTTGTCATGAAACGGCGGCGGCAGCAAAAACTGGAGAAGAGAA TGCAAGAGTTTCGGAGCTCAGATGGGCGGCCTGACTCAGGTGGAACACTGAGGATATATGCAGATAGTTT AAAACCAAATATTCCATACAAGACAATCCTGCTGTCTACTACCGATACCGCAGACTTTGCTGTAGCCGAA TCTTTGGAGAAGTATGGTCTGGAAAAAGAGAATCCCAAGGACTACTGTATTGCCCGGGTTATGCTTCCTC CTGGAGCCCAGCATTCTGATGAGAGAGGTGCTAAGGAAATAATTCTTGACGATGATGAATGTCCTTTACA AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGACTAC ATCCCAAAGAAAATGAAGAAACATGTTGAAGGGAAGTCGTTGAAGGGGAAAGACAGAGCTGACGGGTCTG GCTACGGCTCCGCTCTTCCTCCCGAGAAGCTGCCCTACTTAGTAGAGCTAAGCCCAGGGAGAAGGAATCA CTTTGCCTACTACAGCTATCACACTTACGAAGATGGCTCTGACTCCAGAGACAAGCCAAAGTTATACCGC CTTCAATTAAGTGTGACTGAAGTTGGGACAGAGAAATTTGATGACAATTCTATCCAGCTGTTTGGCCCCG GAATTCAGCCCCATCACTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGA TGCAGAGACCTACGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTGCAGAGTGGCATGAGACTGCAG TTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTCCAAGAGATCTGTGG ATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCTGTTCAGGAGACAACCTTTGAATTGGG AGGAGATGTCCACAGTGGGACAGCATTGCCGGCAAGCAGGAGCACTACTAGACTGGACAGTGACAGGGTG TCCTCTGCCTCTAGCACAGCTGAGCGAGGGATGGTGAAGCCGATGATCCGACTGGACCAGGAGCAGGAAT ATCGACGACGAGAAAACAGGACTCAGGATGCTACTGGGCCTGAACTGATACTGCCTGCCAGCATTGAATT CAGGGAAAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGTTCTACAGTTCATTTT AAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCAGCACAGGCCCGACA TCAGTCCTACAGAGCGTACACATAAGGCCATTGCTGTTGTCAACAAGATGGTGAGCATGATGGAAGGGGT CATCCAGAAACAGAAGAACATTGCAGGAGCACTTGCTTTTTGGATGGCAAATGCATCTGAGCTTCTCAAC TTCATCAAGCAGGACCGAGACCTTAGTCGGATCACGCTGGATGCCCAGGATGTCCTAGCACACTTGGTTC AGATGGCATTTAAGTACTTGGTTCATTGCCTTCAGTCAGAACTTAATAACTACATGCCAGCCTTTCTGGA TGATCCTGAGGAGAACAGTCTGCAAAGACCAAAAATAGATGATGTGCTGCACACACTCACAGGAGCCATG TCCTTGCTGCGGCGCTGCAGAGTCAATGCTGCCTTGACCATCCAGCTCTTCTCTCAGCTTTTCCACTTCA TCAATATGTGGCTGTTCAACAGACTGGTGACTGACCCAGATTCAGGCCTGTGCTCCCACTACTGGGGTGC CATTATCCGCCAGCAGCTGGGGCATATTGAAGCGTGGGCAGAGAAGCAGGGGCTAGAGCTGGCAGCTGAC TGTCACTTGAGCAGAATTGTACAGGCTACAACATTGCTTACGATGGATAAGTATGTACCTGATGACATTC CAAATATAAACAGCACATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGCCTTGTTACAAAATTACCACTG TGCACCCGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTTGTGGCTGTTGCTGAGAACACAGCT GATGAGCTCGCCCGCAGTGATGGCAGAGACGTGCAGCTGGAGGAAGACCCAGACCTACAGCTGCCTTTTC TTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGA CCCTCTGTGCCAGAGAGGATTTTGTAGATTGGTTCCTCACACACGTTCACCAGGTACCTGGACAATATAT TTTGAAGGAGCAGATTATGAAAGTCACCTTATGCGTGAGAACGCAGAACTGGCCCAGCCTCTGAGGAAGG AGCCTGAGATAATCACTGTGACTCTGAAGAAGCAGAATGGAATGGGCCTCAGCATCGTTGCAGCCAAGGG TGCTGGCCAGGATAAACTGGGAATCTACGTTAAGTCGGTTGTCAAAGGAGGTGCTGCAGATGTGGATGGA CGATTAGCTGCTGGTGACCAACTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTTTCTCAGGAAAGGG CAGCAGAACTCATGACAAGAACCAGTTCCGTGGTTACACTGGAAGTTGCAAAGCAAGGTGCCATCTATCA CGGCCTGGCCACCCTCCTTAACCAGCCGTCCCCCATGATGCAAAGAATTTCAGATCGTCGTGGCTCAGGT AAACCCAGACCAAAGAGTGAAGGTTTTGAGCTCTATAATAATTCAGCTCAAAATGGCTCCCCAGAGAGTC CTCAGATGCCTTGGACAGAGTACAGTGAGCCAAAGAAACTGCCTGGGGATGACAGGCTGATGAAAAACAG AGCGGACCACCGTTCCAGCCCCAATGTGGCAAATCAGCCTCCTAGCCCTGGAGGGAAGGGTCCATATACA TCTGGAACAGCAGCTAAGATAACATCTGTCTCCACTGGAAACCTCTGCACTGAGGAGCAGTCCCCTCCAC CCAGACCTGAAGCCTATCCTATCCCTACTCAGACATACACCAGAGAGTATTTTACCTTTCCAGCTTCAAA GTCGCAGGATCGGATGGCTCCTCCTCAGAGCCAGTGGCCAAATTATGAGGAAAAACCACATGTGCACACA GAAAGTAATCATTCCAGTATTGCAATCCAGCGTGTTACCCGTTCCCAGGAAGAGCTTCGGGAAGAAAAGG TTTACCAACTTGAGCGGCATCGAGTAGAGGCAGGCATGGATCGCAAGTGTGACAGTGATATGTGGATCAA TCAGAGCTCCTCAGTGGAATCCAGCACATCCAGCCAGGAACACCTGAACCATTCTTCCAAGTCAGTTACC CCTGCTTCCACTCTGACCAAGAGTGGTCCTGGCCGTTGGAAAACACCAGCAGCGGTGCTGCCCACCCCTG TGGCTGTCTCTCAGCCCATTCGAACAGACCTCCCTCCACCACCACCTCCACCGCCGGTCCACTACACCAG TGAGTTTGATGGTATTCCAATGGATTTGCCCCTCCCACCTCCTCCTGCCAACCAGGCAGGACCCCAGTCT GCTCAGGTGGCTGCTGCAGAGTGGAAAAAGAGAGAGGAGCACCAGAGGTGGTATGAGAAAGAGAAGGCCC GCCTGGAGGAAGAACGAGAGAGAAAGCGCAGGGAGCAGGAGCGGAAGTTGGGGCAGATGCGCTCTCAGAC CCTGAACCCTGCTTCCTTCTCACCTTTGGCCACACAGGCCAAGCCAGAAAAGCCTTCCACACTGCAGAGG CCCCAAGAAACAGTCATTCGGGAGCTTCAGCCCCAGCAGCAGCCCCGCACGATCGAGCGCAAGGATCTGC AGTATATAACCATCAGTAAAGAGGAGCTGTCCTCTGGTGATAGTCTGTCTCCAGACCCCTGGAAACGAGA TGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCACATTGTGGACATGCTAAGCAAGGAGATCCACGAG CTGCAAAACAAGGTGGACCGTACTGCGGAGGAGAGTGACCGCCTGCGGAAGCTTATGCTGGAGTGGCAGT TCCAGAAGAGACTACAGGAGTCCAAACAGAAGGATGAGGATGATGACGAAGAGGAAGATGATGATGTGGA CACCATGCTGATCATGCAGCGTCTGGAGGCCGAGCGGAGAGCCAGGATGCAGGATGAGGAGCGCAGGCGC CAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGCAGAAGATCGAGTAAGACAAGAGGAAGATGGACGCC ATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCGACAGGAGGAAGGGTATTACAGCCG CCTAGAAGCTGAGAGGCGCCGACAGCACGAAGAGGCAGCACGCAGGCTGCTGGAGCCTGAAGAGCCCGGG CTGAGCCGACCTCCACTTCCACGGGACTATGAGCCCCCGTCCTTGTCCTCAGCACCCTGTGCCCCTCCTC CCCCACCTCAGCGAAACGCATCCTACCTCAAAACACAGGTCCTCTCCCCAGACTCGCTGTTCACTGCCAA GTTTGTTGCGTATGATGAGGAGGAGGAGGACTACGGCCCAGCAGGACCAAACTCTTACTCGGGATCTGCA GGCACAGCTGTGGGAGCCTATGATGCCCCTCGGGAAGCGAGAGAGAAACTGACTAGGAGCCAAGACGCAG ACTTACCTGGCAGTTCTGGAGCCCCTGAAAATCTGACATTTAAAGAGCGTCAGCGCCTTTTTTCACAAGG GCAGGATGTGTCTGACAAAGTGAAAGCTTCTCGTAAATTAACAGAGCTCGAGAATGAACTGAACACAAAG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010806
Insert Size	5463 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010806.1, NP_034936.1
RefSeq Size	7401 bp
RefSeq ORF	5463 bp
Locus ID	17356
UniProt ID	Q9QZQ1
Gene Summary	Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm.[UniProtKB/Swiss-Prot Function]

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