Mast2 (NM_008641) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MAST205; Mtssk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224930 representing NM_008641 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGAGCCGCTGCCGCGAGCGGCCGCAGCCGCCACCCGCCCGCCGGGAGGATGCGGCTCCGCGGA CGGCCGAGTTGCCCCAGCCGCAGTCTTTGCCCCCGCGCCGGCGAGCGCCGCCCGGGAGGCAGCTACTGGA GGAGCGGAGCGGGCCTTTGGGGCACGACAGCAGGGAGCAGGATATGGTTACTGGACTTAGTCCTCTGCTC TTCAGGAAGCTTAGTAATCCTGACATATTTGCACCCACTGGAAAAGTTAAACTCCAGCGACAACTTAGTC AGGATGACTGTAAGTTACGGAGAGGAAGCCTGGCAAGTTCTCTGTCGGGTAAGCAGCTGCTCCCTTTGTC CAGCAGTGTACACAGCAGTGTGGGACAGGTAACTTGGCAGTCTACAGGAGAAGCATCAAACCTGGTTCGA ATGAGAAACCAATCCCTTGGACAGTCTGCACCTTCCCTTACGGCTGGCTTGAAGGAATTGAGCCTTCCAA GGAGAGGCAGCTTTTGTCGGACAAGTAACCGCAAGAGCTTGATTGTAACCTCCAGCACATCACCTACGCT ACCAAGGCCACACTCCCCACTCCATGGCCACACAGGTAACAGTCCCTTGGACAGCCCCCGGAATTTCTCT CCAAATGCTCCTGCTCACTTTTCCTTCGTTCCTGCCCGTAGGACTGATGGACGGCGCTGGTCTTTGGCCT CGTTGCCTTCTTCAGGCTATGGAACCAACACTCCTAGCTCTACAGTCTCTTCATCATGTTCCTCACAAGA AAAGCTTCATCAGTTACCTTTCCAGCCAACAGCTGATGAACTACACTTTCTGACGAAGCATTTTAGCACA GAAAACGTACCAGATGAGGAGGGACGTCGGTCCCCAGCCATGCGGCCCCGTTCCCGCAGCCTCAGTCCTG GACGGTCCCCAGTTTCCTTTGACAGTGAAATAATAATGATGAATCATGTGTACAAAGAAAGATTCCCCAA GGCCACTGCACAGATGGAAGAGCGACTAGCTGACTTCATTTCCTCTAACACTCCAGATAGTGTGTTGCCC TTGGCAGATGGAGCACTAAGCTTTATTCATCATCAGGTGATTGAGATGGCCCGAGACTGCCTGGATAAAT CTCGGAGTGGCCTCATTACGTCACACTATTTCTATGAACTTCAAGAGAATTTGGAAAAGCTTCTGCAAGA TGCTCACGAACGCTCAGAAAGCTCAGATGTAGCCTTTGTGATACAGCTGGTAAAAAAGTTGATGATCATC ATTGCTCGCCCAGCTCGCCTCCTGGAATGCCTGGAGTTTGACCCTGAAGAATTTTACCACCTGTTAGAAG CAGCTGAAGGTCATGCCAAAGAGGGACATGGAATTAAATGTGACATTCCCCGCTACATCGTTAGCCAGCT GGGCCTAACTCGGGATCCCTTGGAGGAAATGGCCCAGTTGAGCAGCTATGACAGTCCAGATACTCCAGAG ACAGATGATTCAGTTGAGGGTCGTGGGGTATCTCAGCCATCTCAGAAGACCCCCTCTGAAGAGGACTTTG AAACCATTAAGCTCATCAGCAATGGCGCCTATGGGGCTGTCTTTCTGGTGCGGCACAAGTCCACGCGGCA GCGCTTTGCAATGAAGAAGATTAATAAGCAAAACCTAATCCTACGGAACCAGATCCAGCAAGCATTTGTG GAACGCGACATACTGACTTTCGCTGAAAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCAAGC GTCACTTATGCATGGTGATGGAATACGTAGAAGGGGGAGACTGTGCCACTCTGCTCAAGAACATCGGGGC CCTACCTGTGGACATGGTACGCCTGTACTTTGCGGAAACTGTGCTGGCTTTGGAATACTTACACAACTAC GGCATCGTGCACCGTGACCTCAAGCCTGACAACCTTCTGATTACATCCATGGGACACATCAAACTCACTG ACTTTGGACTTTCTAAAATCGGCCTCATGAGTTTGACAACCAACTTGTATGAGGGTCATATTGAAAAGGA TGCCCGGGAGTTCCTAGACAAGCAGGTATGCGGGACCCCGGAATACATAGCACCTGAGGTGATCCTGCGT CAGGGATATGGGAAGCCAGTGGACTGGTGGGCCATGGGCATAATCCTGTATGAGTTCCTGGTGGGTTGCG TCCCTTTCTTTGGAGACACTCCGGAGGAGCTCTTTGGGCAAGTGATCAGTGATGAGATCGTGTGGCCCGA AGGTGATGACGCGCTTCCCCCAGATGCCCAAGACCTCACTTCCAAACTGCTTCATCAGAATCCACTAGAA AGACTGGGCACAAGTAGTGCCTATGAAGTGAAGCAGCACCCATTCTTCATGGGTCTGGACTGGACAGGAC TTCTACGGCAGAAGGCTGAATTTATACCTCAGCTGGAGTCAGAGGATGACACCAGCTACTTTGATACCCG TTCAGAACGATACCACCATGTGGACTCTGAGGATGAGGAGGAAGTGAGTGAGGATGGCTGCCTTGAGATT CGCCAGTTTTCTTCCTGCTCTCCAAGGTTCAGCAAGGTTTACAGTAGCATGGAACGGCTTTCCCTGCTTG AGGAACGCCGGACACCACCTCCAACCAAGCGCAGCCTCAGTGAGGAAAAGGAAGATCACTCAGACGGCTT GGCAGGACTGAAGGGCCGAGATCGCAGCTGGGTGATTGGGTCCCCTGAGATATTACGGAAGCGGTTATCT GTGTCCGAGTCATCCCACACAGAGAGTGATTCGAGTCCTCCAATGACAGTGCGACATCGGTGTTCAGGCC TCCCAGATGGGCCTCATTGCCCTGAGGAGACTAGTAGCACCCCTCGGAAGCAACAACAGGAAGGTATATG GGTCCTCATTCCCCCATCTGGAGAGGGGTCATCTAGGCCTGTTCCTGAACGACCGTTGGAGAGGCAACTG AAGCTGGATGAGGAGCCTCCTGGCCAAAGCAGTCGGTGTTGCCCAGCCCTGGAGACACGAGGCCGTGGGA CCCCTCAGCTAGCTGAGGAAGCTACAGCCAAAGCCATCAGCGACCTAGCTGTGCGTAGGGCCCGTCACCG GCTGCTCTCTGGGGACTCTATAGAGAAGCGCACCACTCGCCCTGTCAACAAAGTAATCAAGTCAGCCTCA GCTACGGCCCTTTCCCTCCTCATTCCTTCAGAACACCATGCCTGCTCACCATTGGCCAGCCCTATGTCCC CACATTCCCAGTCATCCAATCCATCATCCAGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTTGG CAGCTTGAGGCCTCCCATCATCATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCCATTCGA GTCTACATGGGTGACACTGATGTCTACACCGTACACCACATGGTGTGGCATGTGGAGGATGGTGGTCCAG CCAGTGAAGCAGGGCTTCGTCAGGGTGACCTCATCACCCATGTCAATGGTGAGCCTGTGCATGGGCTAGT CCACACAGAAGTGGTGGAGCTGGTTCTGAAGAGTGGAAACAAGGTATCAATTTCAACAACTCCCTTGGAG AACACGTCAATCAAAGTGGGGCCAGCTAGGAAAGGCAGCTATAAAGCCAAGATGGCCCGAAGGAGCAAAC GGAGCAAAGGCAAGGATGGGCAAGAAAGCCGAAAAAGAAGCTCCCTATTCCGGAAAATCACAAAGCAGGC CTCCTTGCTCCACACCAGCCGCAGCCTTTCTTCCCTTAACCGCTCCTTGTCATCAGGGGAGAGTGGTCCA GGCTCTCCCACACACAGCCACAGCCTCTCTCCCAGATCTCCTCCTCAGGGATACCGGGTAGCCCCAGATG CTGTGCACTCAGTAGGAGGGAATTCCTCGCAGAGCAGCTCTCCCAGCTCCAGTGTGCCCAGTTCTCCTGC TGGCTCTGGACATACACGGCCCAGCTCTCTTCACGGTCTGGCACCCAAGCTCCAACGCCAATACCGCTCA CCACGGCGCAAGTCAGCAGGCAGCATCCCACTGTCACCGTTGGCCCACACCCCTTCCCCACCAGCAACGG CAGCTTCACCTCAGCGTTCCCCATCACCCTTGTCTGGCCATGGGTCTCAGTCCTTTCCTACCAAACTTCA CTTGTCTCCTCCGCTAGGTAGGCAGCTCTCACGGCCCAAGAGTGCAGAGCCACCCCGCTCTCCCCTACTT AAGAGGGTGCAGTCTGCTGAGAAGCTGGCGGCTGCACTGGCAGCTGCTGAGAAGAAGTTAGCACCTTCCC GCAAACATAGTCTTGACCTGCCCCATGGTGAACTAAAGAAGGAACTGACACCCAGGGAAGCCAGCCCTCT GGAGGTAGTTGGAACCAGAAGTGTGCTATCCGGGAAAGGGCCACTTCCAGGAAAGGGGGTACTGCAGCCT GCTCCTTCACGGGCCCTTGGGACCCTACGGCAGGATCGAGCTGAACGCCGTGAGTCACTGCAAAAACAAG AAGCAATCCGGGAAGTAGACTCCTCAGAAGATGACACTGATGAGGAGCCTGAGAACAGCCAGGCCACACA GGAGCCAAGATTGTCCCCCCACCCAGAAGCAAGCCACAATCTACTCCCTAAAGGTTCAGGAGAGGGTACA GAAGAGGACACTTTCTTGCACAGGGATCTAAAGAAGCAGGGCCCTGTACTCTCAGGTCTAGTGACAGGGG CCACACTAGGCTCCCCCCGAGTAGACGTTCCTGGGCTCTCCCCAAGGAAGCTCAGCAGGCCACAAGCCTT TGAGGAAGCTACCAACCCCTTACAAGTCCCTAGCCTGAGCAGGTCTGGACCCACAAGCCCCACCCCCTCT GAAGGCTGCTGGAAGGCCCAGCACCTCCACACACAGGCACTAACTGCACTTTGTCCCAGCTTTTCAGAAC TTACCCCTACCGGTTGTTCTGCTGCCACCTCCACCTCTGGAAAGCCAGGGACATGGTCCTGGAAATTCCT TATTGAGGGTCCAGACAGAGCATCCACGAACAAGACCATAACAAGGAAAGGTGAACCAGCTAACTCCCAA GATACGAATACCACGGTCCCAAATCTTCTGAAGAACCTGTCTCCTGAGGAGGAGAAGCCACAGCCACCAA GTGTGCCTGGGCTGACCCATCCGCTTCTTGAGGTCCCCAGCCAGAACTGGCCATGGGAGTCTGAATGTGA ACAAATGGAGAAAGAAGAACCATCCCTGAGCATCACCGAAGTGCCTGATTCCTCAGGCGACAGGAGGCAG GACATTCCATGCAGAGCCCACCCCCTGAGCCCAGAAACCCGGCCCAGCCTGCTCTGGAAAAGCCAAGAAC TTGGGGGCCAGCAAGATCATCAGGACTTAGCACTGACATCAGATGAGCTCTTAAAGCAAACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008641
Insert Size	5385 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008641.3, NP_032667.2
RefSeq Size	5706 bp
RefSeq ORF	5385 bp
Locus ID	17776
UniProt ID	Q60592
Gene Summary	Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.

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