Ift172 (NM_026298) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930553F24Rik; avc1; Slb; wim
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224902 representing NM_026298 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCGAAGGTGACATGCATGGCCT GGTCCCAGAACAATGCTAAATTTGCTGTCTGCACAGTGGACCGAGTGGTACTTCTGTATGATGAACATGG GGAGCGGAGAGACAAGTTCTCCACCAAACCAGCTGACATGAAGTACGGCAGGAAAAGCTACATGGTGAAA GGCATGGCTTTCTCTCCCGATTCCACAAAAATTGCCATAGGACAGACTGACAACATCATCTATGTCTACA AGATTGGAGAAGATTGGGGAGACAAGAAGGTCATCTGCAACAAGTTCATCCAGACGAGTGCTGTGACTTG TCTGCAGTGGCCTGCGGAATACGTCATTGTCTTTGGACTGGCAGAAGGCAAGGTTCGCTTAGCAAACACT AAAACTAACAAGTCATCTACTATCTATGGGACAGAGTCTTACGTGGTAGCACTGACAACCAACTGCTCTG GGAAGGGGATCCTCTCGGGTCACGCAGACGGCACCATCGTCAGGTACTTCTTCGATGATGAAGGCTCTGG AGAGTCACAGGGAAAGTTGGTTAACCATCCATGCCCACCCTATGCCTTGGCCTGGGCCACCAACAGCATT GTGGCCGCTGGCTGTGACCGGAGGATTGTGGCCTATGGAAAGGAAGGCCATGTGCTGCAGACTTTTGACT ATAGCCGAGACCCTCAGGAGCGGGAGTTCACCACAGCTGCTGCAAGTCCTGGAGGCCAGTCTGTTGTGCT GGGAAGTTATGACAGACTTCGGGTGTTCAACTGGAGTCCTCGAAGAAGCATCTGGGAAGAGGCGAAACCC AAGGAGATCGCTAACTTATACACCGTCACCGCCCTGGCCTGGAAGCGGGATGGCTCACGACTCTGTGCGG GAACACTCTGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGCATTTACAAGAACAAGTTCGA GCTGACGTATGTGGGCCCTAGCCAGGTGATTGTGAAGAACCTGTCTTCAGGGACCAGGGTGGTGCTCAAG TCACACTATGGCTATGAAGTAGAAGAGGTGAAAATCCTGGGAAAGGAACGTTACTTGGTGGCTCACACAT CAGACACACTGCTGCTGGGAGACCTGAACACGAACCGCCTCAGCGAGATAGCCTGGCAGGGATCTGGGGG CAATGAGAAATATTTCTTTGAAAATGAGAATGTGTGCATGATCTTCAATGCTGGAGAGCTGACCCTGGTG GAATATGGGAGTAACGACTCCTTGGGTTCTGTGCGCACCGAATTCATGAACCCTCACCTCATCAGCGTCC GTATCAATGAGCGCTGCCAGCGAGGAATGGAAGATAATAAGAAATTGGCTTATCTTGTTGATATTAAGAC AATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGTACTATCAGTCACGAAAGCCGGGTGGATTGG CTAGAACTTAACGAGACTGGACACAAGCTTCTCTTTAGAGACCGGAAACTTCGGCTGCATCTATATGATA TTGAAAGCTGCTCTAAGACAATGATCCTCAACTTCTGCTCCTACGTGCAGTGGGTTCCGGGGAGTGATGT GCTGGTAGCTCAGAACCGAAACAGCTTATGTGTATGGTACAATATTGAGGCCCCTGAGAGAGTCACCATG TCCTCTATCAGGGGTGATGTTGTCGGTCTGGAGCGAGGTGGTGGGAAGACAGAGGTCATGGTGACAGAAG GCGTGACTACTGTGGCCTACACCCTGGACGAGGGCCTGATTGAGTTTGGAACAGCCATCGATGATGGCAA CTACACCCGGGCTACAGCCTTCTTGGAGACCCTGGAGATGACTCCCGAAACAGAGGCAATGTGGAAAACC TTGAGCAAACTGGCCCTGGAGGCAAGGCAGCTTCACACTGCTGAGAGGTGCTTCTCTGCTTTAGGTCATG TGGCCAAAGCTCGGTTCCTGCATGAGACCAATGAGATTGCAGATCAGGTGTCCAGGGAATATGGTGGAGA AGGAACGGACTTCTATCAGGTCCGAGCTCGTCTCGCCATGCTGGAGAAGAACTACAAACTTGCTGAGATG ATCTTCTTGGAACAGAATGCTGTTGAGGAGGCCATGGACATGTACCAAGAGTTGCACCGCTGGGAGGAAT GCATTGCTGTGGCTGAGGCCAAGGGACACCCAGCCCTGGAGAAGCTGCGCAGAGACTACTATCAATGGTT AATGGACACACAGCAAGAGGAACGAGCAGGTGAACTGCAGGAGAGCCAAGGGGATGGGCTGGCAGCTATC AGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCTGCGCGGCTGGTGCTGACCCGAGAGGAGCTGCTGG CCAACACAGAACTAGTAGAACATATCACCACAGCCCTCATCAAGGGAGAGCTCTATGAAAGGGCAGGTGA TCTCTTTGAGAAGATTCGAAATCCACAGCGGGCCCTTGAGTGTTACTGTAAAGGCAATGCATTCATGAAA GCTGTTGAGCTGGCTCGACTGGCCTTCCCAGTGGAGGTGGTGAGGCTAGAGGAGGCCTGGGGGGACCACT TGGTACAGCAAAAGCAGCTGGATGCGGCCATCAACCACTATATTGAAGCCAGGTGCTCCATCAAAGCAAT TGAGGCTGCCCTGGGTGCCCGCCAGTGGAAGAAGGCAATTTATATATTAGATCTCCAGGATCGCAACACT GCATCTAAATACTATCCTCGTGTGGCTCAACACTATGCGTCTCTGCAGGAGTATGAGATTGCTGAGGAGC TCTACACCAAAGGAGACCGGACCAAAGACGCCATAGACATGTACACCCAGGCCGGCCGCTGGGAGCAAGC CCACAAGCTGGCCATGAAATGCATGCGACCCGAGGACGTGTCCGTGCTTTACATCACACAAGCCCAGGAG ATGGAGAAGCAGGGCAAGTACCGAGAGGCTGAGAGGCTGTACGTGACGGTGGAGGAGCCTGATCTCGCCA TCACCATGTTCAAAAAGCACAAGTTGTATGACGACATGATTCGCCTGGTGGGGAAGCACCATCCAGACCT CCTCAGCGACACACACCTTCACCTGGGCAAGGAGCTGGAGGCAGAAGGCCGACTGCAGGAGGCTGAGTAT CACTACCTTGAGGCCCAGGAATGGAAGGCAACTGTGAACATGTACCGCTCCAGCGGGCTTTGGGAGGAGG CCTACCGGGTGGCCAAAGCCCATGGAGGAGCCAATGCCCACAAACACGTGGCCTATCTGTGGGCCAAAAG CCTGGGAGGAGAGGCTGCAGTTAGACTACTTAACAAGCTGGGACTTCTGGAGGCCACCATTGACCATGCT GCTGACAACTGCTCCTTTGAGTTTGCCTTTGAACTCTCTCGGCTAGCCTTCAAGCACAAAGCCCCAGAGA TTCATCTCAAATACGCCATGTACCTGGAAGATGAGGGCAAATTTGAAGAGGCTGAAGCTGAATTCATCCG AGCTGGTAAACCCAAAGAGGCGGTCCTCATGTTTGTCCACAACCAGGACTGGGAGGCAGCTCAGCGCGTG GCTGAGGCCCACGATCCTGACAGCGTTGCTGAGGTACTTGTAGGTCAAGCCCGTGGGGCCTTGGAGGAGA AGGACTTTCAGAAGGCAGAAGGGTTACTACTTCGGGCCCAGAGACCAGGTCTGGCTCTCAATTATTATAA GGAGGCTGGACTCTGGAGCGACGCTCTGAGAATCTGCAAGGACTATGTGCCTGGCCAGCTAGAGGCCCTA CAGGAAGAGTATGAGCGAGAAGCTACCAAGAAGGGAGGCAGAGGGGTGGAGGGACTTGTAGAGCAAGCAC GGCAGTGGGAACAGGCTGGAGAGTATAGCCGTGCAGTGGACTGCTACCTCAAAGTTCGCGACTCAGGAAG CAGTGGCCTGATGGAGAAGTGCTGGATGAAGGCGGCTGAACTGTCCATCAAGTTCCTGCCTCCCCAGCGC AGTCTGGAAGTCGTCCGGGTTGTAGGGCCCCAGCTGATCGGGATTGGAAAGCACAGTGCTGCTGCAGAAC TCTATCTGAACCTGGACCTTGTCAAAGAAGCAATCGATGCTTTCATTGAGGGGGAAGAGTGGAACAAGGC CAAGCGCGTGGCCAAAGAGCTAGATCCCCGGTATGAGGACTATGTGGATCAGCACTATAAGGAGTTCCTC AAGAACCAGGGCAAAGTGGATTCGCTGGTGGGTGTGGACGTAGTAGCTGCCTTGGACCTGTATGTGGAGC AGGGCCAGTGGGATAAATGCATTGAAACAGCTACCAAGCAGAACTACAAGATTCTGCACAAGTATGTGGC TTTGTATGCAACTCATCTGATCCGAGAAGGTGGCTATGCCCAGGCACTGGCCCTCTATGTGCAACACGGA GCCCCTGCAAACCCACAGAATTTCAACATCTACAAAAGGATCTTCACTGACATGGTTAGCTCTCCTGGGA CCAACAATGCTGAGGCCTACCATAGCTGGGCTGATCTTCGGGATGTCCTCTTCAACCTGTGTGAAAACCT GGTGAAGTCCAGTGAGGCAAACTCTGCAGCGCATGAGGAGTTTGAGATGATGCTGCTGATCTCTCATTAC TACGCTACGCGATCTGCAGCCCAGAGTATCAAACAGCTGGAAACCGTAGCTGCCAGGCTTTCTGTTTCGC TCTTGCGCCACACCCAGTTACTCCCTGCAGACAAGGCCTTTTATGAAGCGGGCACTGCTGCCAAGGAAGT TGGCTGGGAGAACATGGCTTTCATTTTCCTCAATCGGTTCTTGGATCTGACTGATGCAATCGAAGAAGGG ACCCTGGATGCCCTGGACCATTCAGATTTTCAGGATACTGACATTCCCTTTGAGGTGCCGCTCCCAGCCA AGCAGCACGTCCCGGAGGCCCAGAGAGAAGAGGTCCGAGACTGGGTGCTCACAGTCTCGATGGACCAGAG ACTGGAGCAGGTTCTGCCCCGGGATGAGCGAGGTGTCTATGAGGCTTCCCTAGTTGCCGCGAGCACTGGA GTTCGGGCACTGCCCTGCCTCATCACAGGTTACCCCATTTTGAGGAACAAAATTGAATTTAAGCGGCCTG GGAAGGCTGCTAACAAAGACAACTGGAACAAGTTTCTTATGGCCATCAAGACCTCTCACAGCCCAGTGTG CCAGGACGTGCTCAAGTTCATCAGCCAGTGGTGCGGTGGTCTCCCTAGCACCAGCTTTTCCTTTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_026298
Insert Size	5250 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_026298.5, NP_080574.5
RefSeq Size	5440 bp
RefSeq ORF	5250 bp
Locus ID	67661
UniProt ID	Q6VH22
Gene Summary	Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway.[UniProtKB/Swiss-Prot Function]

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