Tjp1 (NM_009386) Mouse Untagged Clone

CAT#: MC224900

Tjp1 (untagged) - Mouse tight junction protein 1 (Tjp1), transcript variant 1, (10ug)



  "NM_009386" in other vectors (2)

CNY 28,030.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms ZO1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224900 representing NM_009386
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG
TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT
TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG
GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC
AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG
TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA
GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG
AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT
CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA
ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG
GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA
AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT
GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC
ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC
GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG
GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA
AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT
GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC
AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG
GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT
TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC
CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG
ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT
TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT
CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG
CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT
TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC
CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG
CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG
AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG
GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA
TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC
TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA
ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC
AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA
CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT
TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG
ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG
AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA
GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCCTCATCTCCAGTCCCTTACCTTTCGC
CTGAAACAACCCCAGCATCATCAGCCTCTGCAGTTAATCATAATGTCAGTGTAACTAATGTCAGCCTGGA
GGAGCCTGCCCCAGCCCCTCCCACCTCGCACGCATCACAGCCTGGTTGTTTAGGAGCACCAAGTGCTGAG
GCAGCTCACGTAGTGCTCAGAGGTGAAGGACCACCATTGCCGCCGCATGCAGACCCAGCAAAGGTGTACA
GGAAGGAGCCGTATTCTGAAGAAATGATGAGACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCC
AGGGCAGAGGCCAGATAAAGAGCCAAATCTAGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCC
AGCAGAGACCTTGAGCAGCCGTCATACAGGTATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACT
ATGACCATCGCCTACGGTTTGAAGATCGAATCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAA
ACAGCCCTACCAACCTCGGCCTTTTGAGAATCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAA
GAGGCTTCAGAACGAGGTTATTTCCAGCGTTTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACAC
GCTATGAGCAGCTGCCTCGAACCTCTACTCTACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCA
CAACAGGTACAGGCCAGAGGCACAGCCCTATTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTAC
TTTGACCAGTACCCGCGAAGTTATGAGCAAGTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACG
AGCCTCTCCATGGTGCTGCAGTTGTCCCTCCTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCC
CTCGGCTACCAAACCACAGCCTCCACCCCCAACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCA
CAGTCTGTGCTCACCAGAGTCAAAATGTTTGAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATG
TGAATGACACTGCCAGCTTTAAGCCTCCGGAAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCC
TAAACCTGTCCCTCAGAGTCAGTTTAGTGAGCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAA
CCTCAAGTGAAGCCACCCGAAGATATTGTTCGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATT
ACCGGAAACAGCTCTCCTACTTTGACCGAAGAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGG
CCATCACTCAGAGCCTGCTAAGCCAGTCCATTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAG
GGAAAACCCGAAACTGATGCTGTGGATAGATCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGC
CTCCTCCTCCTCCGTTGCCCTCACAGTACAGCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACAT
ACATTCCAAGGGCGCCCAGGGTGAAGGCAACTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAA
CCAGACCCACCCCCATCTCAGAGTAAACCAGCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGA
CTTTCTATCCGCAGAAAAGTTTCCCAGACAAAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCAT
CACTCCGGTGTACAATCGATTCACACCAAAGCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTT
GAAAGTCCGAAGTTCAACCATAATCTTCTGCCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAA
CTCCCACTTCCCCAAAAACTCTTATGAAAGCTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGT
TGAAACTTTCTCTGTTCACACAGATAAGCCTAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCT
GTCCCTGTGAGTCCTTCAGCTGTGGAAGAAGATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTC
GTGGCATTTTTAACAGCAATGGTGGTGTGTTGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACA
AGGAGCCATTCCTGAAGGAATTGAGCAAGAAATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCA
CCTTTAGATAAAGAGAAAGGTGAAACTCTGCTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGT
TCCTGAAGCCCGTGGAGCTACGCTTGCCACACTGTGACCCTAAAACCTGGCAAAACAAGTGTCTTCCTGG
AGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTCCTGATTGACCACTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009386
Insert Size 5238 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009386.2, NP_033412.2
RefSeq Size 7054 bp
RefSeq ORF 5238 bp
Locus ID 21872
UniProt ID P39447
Gene Summary Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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