C4a (NM_011413) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Slp; SlpA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224886 representing NM_011413 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCCTCTGGGGGCTGGCTTGGGTGTTCAGCTTCTGTGCCTCATCCCTGCAGAAGCCCAGGTTGC TCCTGTTTTCCCCTTCTGTGGTTAATTTGGGGACTCCCCTGTCAGTGGGGGTACAGCTCCTGGATGCCCC TCCAGGACAGGAGGTAAAAGGATCAGTGTTCCTCAGAAACCCAAAGGGTGGTTCCTGCTCCCCAAAGAAG GACTTTAAGCTGAGCTTGGGAGATGACTTTGTGCTGCTCAGCCTTGAGGTCCCACTGGAAGATGTGAGGA GCTGTGGCCTCTTTGACCTGCGCAGAGCCCCCTACATCCAGCTGGTAGCTCAGTCTCCGTGGCTAAGGAA CACAGCTTTCAAAGCCACAGAGACTCAGGGTGTCAACTTGCTCTACTCTTCCCGACGAGGCCACATCTTT GTGCAGACCGATCAGCCTATCTATAATCCGGGGCAGCGGGTTCGTTATCGGGTCTTTGCACTGGATCAAA AGATGCGCCCATCCACTGATTTCCTCACCATCACAGTGGAGAACTCCCATGGCCTCCGTGTACTCAAGAA GGAGATATTTACTTCCACATCCATCTTCCAAGATGCCTTCACCATTCCAGACATCTCAGAGCCTGGGACC TGGAAGATCTCAGCTAGGTTCTCAGATGGACTGGAGTCCAATAGGAGCACCCACTTTGAAGTGAAGAAGT ATGTCCTTCCCAACTTCGAGGTGAAGATTACTCCTTGGAAGCCATATATCCTGATGGTGCCCAGCAACAG TGATGAAATCCAATTAGACATCCAGGCCAGGTACATCTATGGGAAGCCCGTGCAGGGCGTGGCATACACA CGGTTTGCGCTCATGGATGAGCAAGGGAAGAGGACTTTCCTTCAGGGCCTAGAGACCCAGGCCAAGTTGG TGGAAGGCCGGACCCACATTTCCATCTCAAAGGACCAGTTCCAGGCTGCCCTGGATAAAATCAATATTGG GGTCAGAGACCTGGAGGGGCTGCGTCTCTATGCTGCTACAGCTGTCATTGAGTCTCCAGGAGGAGAGATG GAGGAGGCAGAACTCACGTCCTGGCGCTTTGTATCATCTGCCTTTTCCTTGGATCTCAGCCGCACTAAGC GGCATCTCGTGCCTGGAGCCCACTTCCTGCTGCAGGCCTTGGTCAGAGAAATATCAGGCTCTGAAGCCTC TAACGTTATTGTCAAAGTTTCTGCCACATTGGTGTCAGGCTCTGATTCCCAAGTCCTCAACGTTCAACAG AGCACCAATAGAATTGGCCAAGTCAGCATTTCCTTCCCCATCCCACCAACCGTCACAGAACTTCGGCTCT TGGTGTCTGCGGGCTCCCTCTACCCAACGATAGCCAGGCTCACCGTGCAATCCCCACCTTCAAGAGGCAC TGGCTTTCTTTCTATTGAGCCACTAGACCCTCGGTCCCCTCGTGTGGGGGACACCTTTATCCTAAACTTA CAAGCTGTGGGCATCCCTGCACCTACCTTCTCTCATTACTACTACATGATCATCTCCAGAGGCCAGATCA TGGCTATGAGTCGGGAGGCCCGGAGGACCGTGACCTCTGTCTCCGTGTTGGTGGACCATCAGCTGGCTCC TTCATTCTACTTCGTGGCTTACTTCTATCACCAAGGACACCCGGTGGCCAACTCTCTGCTCATCAACATC CAGCCCAGGGACTGTGAGGGCAAGCTGCAATTGAAGGTAGTTGGTGCCAAGGAGTATCATAATGGGGACA TGATGAAGCTCCAAATTCAAACTGACTCCAAAGCCCTGGTGGCACTGGGAGCTGTGGACACCGCTCTGTA TGCTGTGGGCGGTTGGTCTCACAAACCCCTCGACATGAGCAAGGTCTTTGAAGTAATCGACAGCTACAAC CTTGGCTGTGGTCCTGGAGGTGGGGATGATGCCCCTCAGGTGTTCCAGGATGCTGGTCTGGCCTTTTCTG ATGGTGATCGACTAACTCAAACCAGAGAGGACCTGAGCTGTCCCAAGGAGAAGAAAAGTCGGCAAAAGAG AAATGTTAACTTCCTGAAGGCTCTTAGTGAGAAGTTGGGCCAGTATTCTTCTCCAGATGCCAAGCGCTGC TGCCAAGACGGGATGACGAAGCTGCCCATGAAGCGTACCTGTGAGCAGCGGGCTGCCCGTGTGCCTCAGC AGGCCTGCCGTGAGCCCTTCTTGTCCTGTTGCAAGTTTGCTGAGGACCTTCGCAGGAACCAGACCAGGAG CCAGGCACACCTTGCCCGAAAGGTCCGGGACATGGTGAATTTGATAGAGGAAGATGACATTCTTGTGCGC ACCTCCTTCCCAGACAACTGGCTCTGGAGAGTGGAACCTGTAGACAGCTCCAAACTGTTGACAGTGCGGC TTCCTGATTCTATGACCACATGGGAGATTCATGGTGTGAGCCTGTCCAAAAGCAAAGGTCTGTGTGTAGC CAAGCCAACTCGTGTTCGAGTGTTCAGAAAATTCCACCTTCACCTGCGCCTGCCCATCTCCGTCCGCCGC TTTGAGCAGCTTGAATTACGGCCTGTTCTCTACAACTATCTGAATGATGACAAGAACGTGAGTGTCCATG TGACCCCAGTGGAGGGCCTGTGCATGGCTGGTGGTGGAATGATGGCCCAGTGGGTGATAGTGCCTGCAGG CTCTGCCCTGCCTGTGGCCTTCTCTGTGGTACCCACAGCTTCTACCAACGTGCCCCTGAAATTGGTGGCT AAAGGGACTTTGGATTCAAGCGATTCTGTGTCTAAGATTCTCCAAATTGAGAAGGAAGGAGCCATCCACA GAGAAGAGATAGTCTACAACCTCGACCCCCTAAATAACCTAGGTCAGATGTTGGAGATACCTGGCAGCTC GGATCCCAACATAGTCCCTGAAGGAGACTTCAGCACCTTTGTCAAGGTTACAGCCTCTGAACCCTTGGAG ACTCTGGGCTCTGAAGGAGCTTTGTCCCCAGGAGGCGTGGCCTCCCTCCTGAGGCTTCCCCGGAGCTGCG CAGAGCAAACCATGATCTATTTGGCTCCTACCCTGACTGCTTCCAACTACCTGGACAGGACAAAACAGTG GAGCAAACTGTCCCCTGAGACCAAGGACCATGCTGTGCATCTGATCCAGAAAGGCCACGTTCGGATCCAG CAGTTTCGGAAGAAAGATGGCTCTTTTGGGGCTTGGTTACACCGGGACAGCAGCACCTGGCTGACTGCCT TTGTGCTGAAGATTCTGAGTTTGGCCCAGGAACAGGTGGGCAACTCCCCGGAGAAGCTGCAGGAGACGGC TAGCTGGCTGCTGGCCCAGCAGCTGGGTGATGGCTCCTTCCACGACCCATGTCCAGTCATCCACAGAGCA ATGCAGGGGGGCTTGGTGGGGTCCAATGAGACGGTGGCACTGACCGCCTTTGTGGTCATTGCCCTTCACC ACGGGCTGAACGTCTTCCGGGAGGGGCATGCGAAGCAGCTGAAGAACAGAGTGGAAGCCTCCATCACCAA GGCAAACTCTTTCTTGGGGCAGAAGGCAAGTGCTGGGCTCCTGGGTGCCCATGCCGCCGCCATCACAGCC TATGCCCTTACGCTGACCAAGGCCTCGGAGGACCTGCGGAATGTTGCCCACAACAGCCTGATGGCCATGG CTGAGGAAACGGGGGAAAACCTCTACTGGGGCTTAGTCCTTGGCTCTCAGGACAAAGTTATGTTGCGCCC CGCAGACCCCCGTAGCCCAACAGAACCTGTGCCCCAGGCCCCAGCCTTGTGGATCGAAACCACAGCCTAT GCCCTGCTCCACCTGCTTCTGCGGGAGGGAAAGGGAAAAATGGCTGACAAGGCTGCATCCTGGCTCACCC ACCAGGGAAACTTCCACGGGGCATTCCGCAGTACCCAGGACACTGTGGTCACCCTGGATGCCCTGTCTGC CTACTGGATCGCTTCACACACCACTGAGGAGAAAGCACTGAACGTGACGCTCAGCTCCATGGGCCGCAAT GGGCTTAAGACCCACGGGCTACACTTGAACAACCACCAAGTCAAGGGCCTGGAGGAGGAGCTAAAGTTCT CCCTGGGCAGCACAATCAGTGTCAAGGTGGAAGGAAACAGCAAAGGCACCTTGAAGATCCTTCGTACCTA CAACGTCCTGGACATGAAGAACACCACATGCCAGGACCTTCAGATAGAAGTGAAGGTCACAGACGCTGTG GAATATGCATGGAGCGCCTACGAAGACTATGAAGACGACTATAACATGCCAGCCACAGATGATCCCAGCG TTCCCTTGCAGCCTGTCACGCCCCTGCAGCTATTTGAGGGTCGTCGGAGCCGCCGCAGGAGGGAGGCCCC CAAGGTGGCTGAAGAGCGGGAGTCCAGAGTTCACTACACTGTGTGTATCTGGCGCAATGGCAAGCTGGGG CTGTCTGGCATGGCCATCGCAGACATCACCCTCCTGAGTGGATTCCACGCCCTGAGGGCTGACCTGGAGA AGCTGACCTCTCTCTCTGACCGTTATGTGAGTCACTTTGAGACTGACGGGCCCCATGTCCTGTTGTACTT TGACTCGGTCCCTACCACCCGGGAGTGTGTGGGCTTTGGAGCCTCACAGGAGGTGGTTGTGGGACTGGTG CAGCCAGCCAGCGCTGTCCTGTATGACTACTACAGCCCTGATCACAAGTGCTCTGTGTTTTATGCTGCAC CCACCAAGAGCCAGCTCCTGGCCACACTGTGCTCTGGAGATGTATGCCAGTGTGCCGAGGGGAAGTGCCC TCGACTGCTAAGGTCACTGGAGCGAAGGGTGGAGGACAAGGACGGCTACCGGATGAGGTTCGCCTGCTAT TATCACCAAGTGGAGTATGGCTTCACGGTTAAGGTTCTTCGAGAAGATGGCAGAGCTGCCTTCCGTCTCT TTGAGTCCAAGATCACCCAAGTCCTGCATTTCAGAACGGACACCATGGCCTCCATAGGTCAGACCCGCAA CTTCCTGAGCCGGGCCTCTTGCCGCCTTCGTTTGGAGCCTAACAAAGAGTACTTGATCATGGGGATGGAC GGGGAAACCAGTGACAACAAGGGAGACCCCCAGTACTTGCTGGACTCAAATACCTGGATTGAGGAGATGC CTTCAGAACAAATGTGCAAGAGCACCCGCCATCGGGCAGCCTGTTTCCAGCTCAAAGACTTCCTGATGGA GTTCAGCAGCCGGGGGTGCCAGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011413
Insert Size	5208 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011413.2, NP_035543.2
RefSeq Size	5369 bp
RefSeq ORF	5208 bp
Locus ID	625018

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