Mbd5 (NM_029924) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430004D19Rik; AA536666; AI426407; C030040A15Rik; Gm1630; OTTMUSG00000012483
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224878 representing NM_029924 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGGCAAAGAGTGTGACGGAGGAGACAAGGAAGGAGGTCTTGCAGCTATCCAGGTTCCTGTGG GTTGGCAGCGGCGTGTGGACCATAATGGCGTGCTGTACATCAGTCCCAGTGGGTCTTTGTTATCTTGCTT GGACCAGGTTAAGACATACCTGCTCACTGACGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTATTCTT CCCAAGGTGTTTAATTTTGATCCTGGAGCTGCTGTGAAGCAGAGAACTGCAGAAGATGTTAAGGCAGATG ATGATGTCACAAAGCTATGCATACATAAAAGAAAGATCATTGCAGTGGCCACACTCCATCAAAGCATGGA GGCCCCGCATCCTTCTCTGGTGCTCACCAGTCCTGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCA CGGGCGGCAACTCCAAGATCAGTAAGAAACAAGTCTCATGAAGGAATTACAAATTCTGTAATGCCAGAAT GTAAGAATCCTTTCAAGTTAATGACTGGATCATCAAATGCCATGGGAAGGCTATACATGCAAGACCTGCC TGGAAGCCAGCAGCAGGAGCTCCACCCTGTCTACCCCCGCCAGAGATTGGGCAGTAGTGAGCATGGACAG AAATCTCCATTTCGTGGCAGCCATGGAGGCCTGCCTAGCCCAGCATCATCAGGGTCTCAGATCTATGGAG ATGGCTCAATCTCTCCAAGGACTGACCCGCTCGGAAGCCCTGATGTCTTCACAAGAAATAACCCTGGTTT TCATGGAGCTCCCAATTCCAGCCCTATTCACCTAAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA CATGGTTCTCCCGTACAGTCATCCTGTGCAATGGCTGGAAGAACTAATATACCTCTTTCCCCAACCTTGA CTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAATATGGAAATACCACGAGCAAT GTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCTCCACCTTCTTGTGCTCTTCAGAAAAAGCCA TTAACATCTGAGAAAGATCCACTTGGCATTCTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTA TTATCATTAATCCAACCAGTTTCCATTCAAATGTCCACTCTCAGGTACCTGTGATGAATGTAAGCATGCC TCCTGCTGTTGTTCCTTTGCCAAGTAATCTCCCTTTGCCAACTGTAAAACCTGGTCATATGAATCATGGG AGTCATGTACAAAGAATTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACATCCCCAGTGC ACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCACGCTCATCTTCCACATCATC AGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAGGCCACTTGTAGTGGCATAAAGGTTCCACCT AGGTCACCAAGGTCAACAATAGGTTCCCCAAGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCTGATG GACATCATCAGTACAAGGATATCCCTAACCCATTAATTGCTGGAATGAGTAATGTACTCAATACCCCAAG CAGTGCAGCTTTTCCTACTGCACCTGCCGGAAATGGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTCTCAGCAGCAG CCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGCAGCAGTAACAATTCTGGAGC TGTTGCCAGCAGCGGCAACACTGAAGGACACAGCACTTTAAACACCATGTTCCCTCCAACTGCCAACATG CTTCTCCCAACAGGTGAAGGTCAAAGTGGTCGAGCAGCACTAAGAGATAAGTTGATGTCTCAGCAAAAAG ACTCATTACGGAAAAGAAAGCAGCCACCCACCACAGTGTTGAGTTTGCTCAGACAGTCCCAAATGGATAG TTCTGCAGCTCCTAAACCTGGACCTGACTTGCTAAGGAAGCATGGGCAGGGGTCATTTCCCATCAGTTCA ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACCCCGGGTTGTG GGGGATCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTCCAGATCCTAATATGAATTCCAC TGTTCTTCAGAATTCACTGACACAGAGCATACCTTTAAGAGGGGAAGCCGTGCACTGCCACAATGCAAAC ACTAACTTTGTTCACAGTAACAGTCCAGTCCCCAACCACCATCTTGCAGGTTTAATAAATCAGATTCAGG CTAGCGGGAACTGTGGGATGCTCAGTCAGTCGGGCATGGCTTTAGGAAACTCATTACATCCCAATCCACC TCAGTCAAGAATTTCAACGTCCTCCACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACA AGTTCTGAAGCAGGTGGTTCAAGCCTGCCGTCCTCCATAGCTATAGCTGGCAGCAACCACCCTGCCATCA CAAAGACAACATCTGTTCTCCAAGATGGTGTCATAGTCACCACCGCAGCTGGAAACCCACTGCAGAGTCA GCTGCCCATTGGGAGTGACTTTCCTTTTGTTGGCCAAGAGCATGCCCTTCATTTTCCATCCAACAGCACC GCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGCTCTCTACCTATCTCTTTGCCAGTGA ATCAACAGCATCTCCTAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAGGCAAGTCTGA GATCAACCTCCACCCTTTAGGTTTTCTCAACCCGAATGTAAACGCTGCTTTAGCTTTCCTCTCCGGTGAC GTGGATGGGCAGGTATTACAGCCTGTCCACTTTCAGCTCTTAGCAGCCCTGCTTCAGAACCAAGCCCAAG CAGCTGCCATGCTTCCCCTGCCGTCTTTCAATCTGACCATCTCAGATCTTTTGCAACCGCAAAATACCCC TTTACCCTTATTAACACAGATGACAGCCCCACCAGACCGTTTGCCAAGCAATCAGTCAGACAACAGCCGA GCTGATACCCTTTTAGGCAGCCCCCTGGGAAACCCTTTAGCAGGCAGTGACACTACTTTTAACCCCCTGC TCCTCCCAGCTGTCACTGGGGCCTCAGGATTAATGGCCTTGAATCCCCAGCTGTTGGGGGGTGTCCTGAA CTCGGCATCGGCCAACACCGCTATTCATCCAGAGGTTTCCATAGCAACCTCCTCCCAGGCAACCACTACC ACAACCACTACATCATCAGCAGTGGCAGCACTGACTGTCTCAACACTTGGTGGGACAGCAGTGGTGTCAA TGGCAGAAACATTGCTGAATATATCTAATAATGCTGGGAATCCACCTGGTCCAGCTAAACTCAACAGTAA CTCTATGGTGCCACAGCTACTTAACCCTCTACTGGGGACAGGTCTGCTTGGTGATATATCATCCATAAAC AATTCATTGAATAACCATCAACTGACTCATCTACAGTCGCTACTAAACAGCAATCAGATGTTTCCTCCAA ATCAGCAGCCGCAGCAACACCTTCTCCAGGGACACCAGAATCTCCAGGCCTTCCAAGGACAACCCACAGT CCCTTGCCCAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTCCAGGTGAGGATGCAAGGAGAT GCAGCTCTTCTAAATAAAAGAATAAGCACTCAGCCTGGGCTCACAACACTTCCTGAAAACCCAAACCTTG CACTTCCACATTTCCAAGATACACCCTGTGAGCTGCAGCCAAGGATTGACCTTGGTCAGCCTATGAAGGA CGGCCTCGTTATGGGTGGCCAAGGAGATGCCTCCGTAGACGCCATTTACAAAGCAGTTGTGGATGCAGCC AGCAAAGGAATGCAGGTTGTCATCACCACTGCTGTCAACAGCACGACTCAAATCAGCCCTATCCCAGCCC TGAGCGCCATGAGTGCCTTCACTGCCTCCATTGGTGACCCATTAAATCTGTCCAGTGCTGTGAGCGCTGT CATCCACGGGCGGAACATGGGAGGAGTGGATCATGATGGCAGGCTGAGGAATGCGAGAGGGGCACGGCTA CCCAAGAACATAGACCATGGGAAGAACTCCAGCGAAGGAGACGGGTTTGAGTGTTTCAAGTCAGCGAGCT GCCACACATCCAGAAAACAGTGGGATGGGGAGCAAAGTCCCAGAGGGGAGCGCAACAGGTGGAAGTATGA GGAGTTTTTAGATCATCCAGGCCATATCCACAGTAGTCCTTGCCACGAAAGGCCCAACAATGTCTCTACA CTGCCATTTCTAGCTGGGGAACAGCACCCGATACTGTTACCACCAAGAAACTGTCAAGGTGATAAGATTC TGGAGGAAAATTTCAGGTATAATAATTACAAAAGAACTATGATGAGTTTTAAAGAGAGACTAGAGAGTAC TGTGGAGAGATGTACACACATTAACGGAAACAGACCTCGTCAGAGTCGGGGCTTTGGAGAGCTGCTAGGC ACTGCAAAACAAGACCTAGTTCTAGAGGGGCAGTCTCCGGGTTCTTCAAATAGTTTGGAAAGTTCTCTGG TCAAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAACCATTAATGGGTGTGTGCCTAGTCCCTC AGATGCTAAAAGCATTAGTAGTGAAGACGACCTACGGAATCCAGACTCCCCCTCCTCACATGAGTTGATA CACTATAGACCAAGGACGTTCAACGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGC CTGGGAAATTCATAAGAGAAGACGACGTTCACAATTCATGCCAACAAAGCCCCGAGGAAGGGAAGGTGGA GCCCGAGAAGTTGAAGACTCTCACAGAAGGTTTAGAAGCCTACAGCCGCGTCCGGAAAAGGAGCAGAAAA AGTGGGAAACTAAATAACCATTTAGAAGCTGCTATTCACGAGGCCATGAGCGAACTGGACAAAATGTCTG GGACTGTTCACCAAATCCCACAGGGTGACAGACAAATGAGGCCCCCCAAACCCAAGAGGAGGAAGATCTC CAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029924
Insert Size	5187 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029924.2, NP_084200.1
RefSeq Size	7055 bp
RefSeq ORF	5187 bp
Locus ID	109241
Gene Summary	Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This variant has an inferred 5' UTR exon combination based on the mRNA AK155574.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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