Setd1a (NM_178029) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC010250; KMT2F; mKIAA0339; mNSC1; Nsccn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224870 representing NM_178029 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGGAAGGTGGGGGAGACGGGCAGAAAGCCCCGAGCTTCCAGTGGCGGAACTACAAGCTCATAG TGGATCCTGCTTTGGACCCTGCTTTGCGCAGGCCTTCCCAGAAGGTGTACCGCTATGACGGAGTCCACTT CAGTGTCAGTGACTCAAAGTATACCCCCGTGGAAGACCTCCAGGACCCCCGCTGTCATGTCAGGTCCAAA GCCAGAGACTTTTCCCTCCCGGTTCCTAAGTTTAAGCTGGATGAGTTCTATATTGGACAGATACCACTGA AGGAAGTAACTTTTGCGAGGCTCAATGACAATGTGAGGGAAACCTTCCTGAAGGATATGTGCCGGAAATA TGGAGAGGTGGAAGAAGTGGAGATCCTTCTTCATCCCCGCACTCGAAAGCACCTAGGCCTGGCCCGTGTG CTCTTCACCAGCACTCGGGGCGCCAAGGAAACTGTCAAAAACCTCCACCTCACCTCTGTCATGGGCAACA TCATTCATGCCCAGCTTGATATCAAAGGCCAACAACGAATGAAGTACTATGAATTGATTGTCAATGGCTC TTACACTCCTCAGACTGTGCCCACTGGGGGCAAGGCCTTGAGTGAGAAGTTCCAGGGTTCTGGTGCAGCC GCTGAGACGACTGAAGCCCGCCGACGGTCTTCCTCGGACACAGCTGCCTATCCAGCAGGCACTACTGTGG GGGGCACCCCTGGCAATGGCACTCCCTGCTCCCAAGATACAAATTTCTCCAGCAGCCGACAAGATACTCC ATCTTCCTTTGGCCAGTTCACCCCTCAGTCCTCCCAAGGAACCCCCTACACATCTCGGGGTAGCACCCCC TACTCTCAGGACTCTGCCTACTCCAGCAGCACCACTTCAACCTCCTTCAAACCCCGGCGGTCAGAGAACA GCTATCAAGATTCTTTTTCTCGCCGCCATTTCTCTACATCTTCAGCCCCTGCAACCACCGCTACTGCCAC CTCAGCAACTGCTGCAGCTACTGCAGCCTCCTCCTCATCTTCCTCCTCCTCGTCATCGTCCTCCTCCTCG TCGTCTTCTTCCTCAGCCTCTCAGTTTCGTGGTTCTGACTCTAGCTACCCAGCATATTATGAAAGCTGGA ATCGTTACCAGCGCCATACTTCCTATCCACCTCGCCGGGCAACTAGAGAGGACCCTTCTGGGGCTTCATT TGCCGAAAATACGGCTGAGCGCTTCCCACCTTCCTATACCTCCTATTTGGCCCCTGAGCCTAACCGATCC ACTGACCAAGACTACCGGCCTCCTGCCTCAGAGGCCCCACCTCCAGAACCTCCAGAACCTGGTGGTGGTG GTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGGGCACCCAGCCCTGAGAGAGA AGAAGCCCGGACTCCCCCACGCCCAGCTTCCCCTGCACGCTCTGGCTCCCCAGCCCCAGAGACCACCAAC GAGAGTGTGCCCTTTGCTCAGCACAGCAGTTTGGATTCCCGTATTGAAATGCTGCTGAAGGAACAACGCT CCAAGTTTTCTTTCTTGGCTTCTGATACAGAGGAAGAGGAAGAGAACAGCAGTGCAGGCCCTGGGGCTAG AGATGCAGGGGCTGAGGTGCCTTCTGGGGCAGGTCATGGGCCCTGTACACCTCCTCCTGCACCAGCTAAT TTTGAAGATGTAGCACCTACAGGGAGTGGAGAGCCAGGGGCTGCCCGGGAGTCACCAAAGGCCAATGGAC AGAACCAGGCTTCTCCATGTTCTTCCGGAGAAGATATGGAGATCTCTGATGATGACAGGGGTGGCTCACC ACCTCCAGCCCCAACACCCCCCCAGCAGCCGCCACCGCCACCACCTCCCCCTCCTCCCCCCCCTCCTCCC TACCTTGCTTCCCTTCCCCTTGGCTATCCTCCCCACCAACCTGCCTACCTCCTCCCACCCCGCCCAGATG GACCACCCCCCCCTGAGTACCCTCCGCCACCTCCTCCACCACCACCCCACATCTATGACTTTGTGAACTC CCTGGAACTTATGGATCGACTTGGGGCACAGTGGGGAGGGATGCCCATGTCCTTCCAGATGCAGACCCAG ATGTTAACACGGCTCCACCAGTTACGGCAGGGCAAGGGATTGACTGCAGCCTCAGCTGGTCCTCCTGGCG GGGCTTTTGGCGAGGCCTTTCTCCCTTTTCCACCTCCACAAGAGGCAGCCTATGGCTTGCCCTATGCTCT GTACACACAAGGGCAAGAAGGTCGTGGGTCGTACTCCCGGGAGGCCTACCACCTGCCCTTGCCTATGGCA GCCGAGCCCCTGCCCTCTTCCTCAGTCTCGGGAGAGGAGGCGCGGCTGCCTCATAGGGAGGAAGCAGAGA TAGCAGAAAGCAAGGTCCTACCATCAGCAGGCACAGTGGGTCGTGTGCTGGCTACCCTTGTCCAAGAGAT GAAGAGCATTATGCAGCGAGACCTTAATCGAAAGATGGTCGAGAATGTGGCCTTTGGAGCCTTTGACCAG TGGTGGGAGAGCAAGGAAGAGAAGGCCAAGCCATTCCAGAATGCAGCCAAACAGCAAGCCAAGGAAGAGG ACAAAGAAAAGATGAAGCTCAAAGAGCCAGGCATGCTGTCCCTCGTAGACTGGGCCAAGAGTGGCGGTAT CACAGGCATTGAGGCCTTTGCCTTTGGGTCAGGGCTTCGAGGGGCCCTGCGGTTGCCTTCATTCAAGGTA AAACGGAAAGAGCCATCAGAAATTTCAGAGGCAAGTGAGGAAAAGAGGCCCAGGCCCTCTACTCCAGCTG AGGAAGATGAAGATGATCCTGAGCGAGAGAAGGAAGCTGGAGAACCAGGACGTCCAGGGACCAAGCCTCC GAAGCGAGATGAAGAACGGGGCAAGACCCAAGGCAAACACCGAAAATCCTTTACTCTGGACAGTGAAGGG GAGGAGGCGTCCCAGGAGTCTTCCTCAGAGAAGGATGAGGATGATGATGATGAAGATGAGGAAGATGAAG AACAGGAGGAAGCTGTGGATGCCACCAAGAAGGAAGCAGAGGCATCGGATGGTGAGGATGAGGACAGTGA CTCGTCCTCCCAGTGCTCTCTGTATGCGGATTCAGATGGTGAAAATGGCAGCACATCAGACTCCGAGAGT GGCAGCTCGTCTTCCTCTTCCTCATCGTCTTCCTCCTCTTCCTCCTCTTCCTCATCAGAGTCCTCCTCTG AGGAAGAGGAGCAGTCAGCAGTCATTCCCTCAGCTTCACCCCCCAGAGAAGTTCCAGAGCCCCTTCCAGC TCCTGATGAGAAGCCTGAGACAGACGGGCTTGTAGATTCCCCTGTCATGCCTCTCTCTGAAAAGGAGACA TTGCCCACACAGCCTGCAGGCCCTGCTGAAGAACCACCTCCTAGTGTACCACAGCCTCCAGCAGAGCCAC CCGCCGGACCGCCAGACGCTGCCCCCCGCCTGGATGAGCGCCCCTCTTCTCCTATCCCTCTCCTACCCCC ACCCAAGAAACGCCGGAAAACTGTTTCCTTCTCTGCTGCAGAGGAGGCACCAGTCCCAGAACCTTCCACA GCTGCCCCACTGCAGGCCAAGTCTTCTGGTCCAGTCTCTCGAAAAGTCCCCCGGGTCGTAGAGCGGACCA TTCGTAACCTCCCCCTGGACCATGCGTCTCTGGTCAAGAGTTGGCCTGAGGAGGTCGCCCGAGGGGGTCG GAATCGAGCTGGAGGTCGTGTGCGCTCTACTGAAGAAGAGGAGGCCACTGAGTCAGGGACAGAAGTAGAC CTGGCAGTACTGGCTGACCTGGCCCTGACCCCAGCCCGACGTGGGTTGGCTACCCTACCCACTGGTGACG ACTCAGAGGCCACAGAGACCTCAGATGAGGCTGAGCGCCCAAGCCCTCTACTCAGCCACATCCTCTTGGA ACACAACTATGCCCTAGCCATCAAGCCCCCGCCTACCACACCAGCACCTCGGCCCCTGGAGCCAGCTCCT GCCCTTGCTGCACTCTTCAGCTCTCCAGCTGATGAAGTCTTAGAGGCCCCTGAGGTGGTGGTGGCTGAGG CAGAGGAGCCAAAGCAACAGCTGCAGCAGCAGCATCCAGAGCAGGAAGGAGAAGAGGAGGAGGAAGATGA AGAGGAGGAGTCCGAGTCTTCAGAAAGCAGCAGCAGCAGCAGCAGTGATGAGGAAGGGGCAATCAGGAGG CGTAGCCTCCGCTCCCACACTCGAAGAAGAAGGCCACCACTCCCACCCCCACCCCCACCTCCTCCTTCCT TTGAGCCGAGAAGTGAATTTGAGCAGATGACCATCTTGTATGATATTTGGAACTCAGGCCTTGACTTGGA AGACATGAGCTATTTGCGACTCACATATGAACGGCTGCTGCAGCAAACCAGTGGCGCTGACTGGCTTAAT GACACCCACTGGGTCCAGCACACAATCACCAACCTAAGCACTCCAAAGCGCAAAAGACGGCCCCAAGATG GGCCCCGGGAGCATCAGACAGGCTCTGCACGCAGCGAAGGTTATTACCCCATTAGCAAGAAGGAGAAAGA TAAATACTTGGATGTGTGCCCCGTCTCGGCCCGGCAGCTGGAAGGTGGAGACACTCAGGGGACTAATCGG GTGCTTTCTGAGCGACGGTCAGAGCAGCGGCGGTTACTAAGCGCTATTGGCACCTCAGCCATCATGGACA GTGATCTGCTAAAGCTAAACCAGCTCAAGTTTCGGAAGAAGAAACTCCGATTTGGCCGGAGCCGTATCCA TGAGTGGGGTCTGTTTGCCATGGAACCCATTGCCGCTGATGAGATGGTGATCGAATATGTTGGCCAGAAC ATCCGCCAGATGGTAGCGGACATGCGGGAAAAGCGCTACGTGCAGGAGGGCATTGGCAGCAGCTACCTTT TCCGGGTGGATCACGACACCATCATTGATGCCACCAAGTGTGGCAACCTCGCCAGGTTCATCAACCACTG CTGCACGCCCAACTGCTATGCGAAGGTGATCACCATTGAGTCTCAGAAAAAGATTGTCATCTACTCGAAG CAGCCCATCGGCGTGGATGAGGAGATCACCTACGACTACAAGTTCCCACTAGAAGACAACAAGATTCCGT GTCTGTGCGGCACTGAAAGTTGCCGTGGCTCCCTCAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_178029
Insert Size	5151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178029.3, NP_821172.2
RefSeq Size	5934 bp
RefSeq ORF	5151 bp
Locus ID	233904
UniProt ID	E9PYH6
Gene Summary	Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.[UniProtKB/Swiss-Prot Function]

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