Trpm3 (NM_001035240) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224867 representing NM_001035240 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGCGAGAGTATCCAGGTTTCGGTTGGATCTATTTTAAGGGAAAC CTGCCCCCGGACTACAGAATCAGCCTGATAGACATTGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTT ACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCCTCTACCACAATCTCTTTGGCCCCAAAAGGCCCAA AGCCTTGAAACTGCTGGGAATGGAGGATGATATTCCCTTGAGGAGAGGACGGAAGACAACTAAGAAGCGT GAGGAAGAGGTGGACATCGACTTGGATGATCCTGAGATCAACCACTTTCCATTCCCCTTCCATGAGCTGA TGGTGTGGGCCGTCCTCATGAAGAGACAAAAGATGGCCCTGTTCTTCTGGCAACACGGAGAGGAGGCCAT GGCCAAAGCCCTGGTGGCCTGCAAGCTCTGCAAGGCCATGGCTCACGAGGCTTCTGAGAATGACATGGTC GATGACATTTCCCAGGAGCTGAACCACAACTCCAGGGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACC AGTCCTACAAACAGGATGAGCAGCTGGCCATGAAGCTGCTGACGTATGAGCTGAAGAACTGGAGTAATGC CACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCGTGACTTCATTGCACACACGTGCAGCCAGATGTTA CTTACTGACATGTGGATGGGACGGCTCCGGATGAGAAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTC TACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGATGACATGCCCTATATGACTCAGGCCCA GGAGATTCATCTCCAAGAGAAGGAGCCGGAGGAGCCAGAGAAGCCCACAAAGGAAAAAGATGAAGAGGAC ATGGAGCTAACAGCAATGTTGGGGCGAAGCAATGGGGAATCATCCAGAAAGAAAGATGAAGAAGAAGTTC AAAGCAGGCACCGGCTAATCCCCGTGGGCCGAAAAATCTATGAGTTCTACAACGCACCCATCGTGAAGTT CTGGTTCTACACTCTGGCGTATATTGGATACCTGATGCTCTTCAACTACATCGTGTTAGTGAAGATGGAG CGCTGGCCTTCCACTCAGGAATGGATTGTCATCTCCTACATTTTCACTCTGGGAATAGAGAAGATGAGAG AGATCCTGATGTCGGAGCCGGGCAAGCTGCTGCAGAAGGTGAAGGTGTGGCTTCAGGAGTACTGGAACGT CACAGACCTCATCGCCATCCTTCTCTTCTCGGTGGGAATGATCCTTCGTCTTCAAGATCAGCCCTTCAGG AGTGACGGGAGGGTCATCTACTGTGTGAACATCATTTATTGGTATATCCGTTTGCTGGACATCTTCGGCG TGAACAAGTATCTGGGCCCATATGTGATGATGATTGGGAAAATGATGATAGACATGATGTACTTTGTCAT CATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCA TCTTGGAAACTGGCCAAGAATATCTTCTACATGCCTTATTGGATGATTTATGGGGAAGTGTTTGCTGACC AGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCATGCAA GACAGGAGCATGGATTGTCCCGGCCATCATGGCCTGCTACCTCTTGGTGGCGAACATCCTTCTGGTCAAC CTCCTTATTGCCGTCTTCAACAATACATTTTTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAATTTC AGAGGTACCAGCTCATCATGACTTTCCACGAGAGGCCGGTTCTGCCCCCACCGCTCATCATCTTCAGTCA CATGACCATGATCTTCCAGCATGTGTGCTGCCGGTGGAGGAAGCATGAGAGTGACCAGGACGAAAGGGAC TACGGCCTGAAACTCTTCATAACTGACGATGAGCTCAAGAAAGTACACGATTTTGAAGAGCAGTGCATAG AGGAATATTTCCGAGAGAAGGATGATCGCTTCAATTCGTCCAACGATGAGAGGATACGTGTTACATCAGA AAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGTTAATGAGAGAGAACATTCCATGAAGGCTTCACTC CAGACCGTGGACATCCGGCTAGCACAGCTAGAGGACCTCATCGGGCGCATGGCCACCGCCCTGGAGCGCC TGACTGGTCTGGAGCGGGCAGAGTCTAACAAAATCCGCTCAAGGACCTCCTCAGACTGCACAGATGCAGC CTACATCGTCCGCCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTATAGAC CCTGCAGGTGAGGAGACCATATCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCT ATTCAGTCAATGTGAAAGACAAAGGTGGGATAGAAAAGTTGGAAAGCATTTTCAAAGAAAGGTCCCTGAG CTTACACCGAGCTACTAGCTCCCACTCAGTAGCCAAAGAACCCAAAGCTCCTGCAGCCCCTGCAAACACC TTGGCCATTGTTCCTGACTCCAGAAGACCATCATCTTGCATAGACATCTATGTCTCTGCCATGGACGAGC TCCACTGTGATATAGAGCCTCTGGATAATTCCATGAACATCCTTGGGCTGGGTGAGCCAAGCTTTTCAGC TCTAGCACCTTCCACAACCCCGTCAAGTAGTGCCTATGCAACGCTCGCACCTACAGACCGACCTCCCAGT AGGAGCATTGATTTTGAAGACCTCACCTCCATGGACACTAGATCTTTTTCTTCAGACTATACACACCTCC CAGAATGCCAAAACCCCTGGGACACAGACCCTCCAACGTACCATACCATCGAGCGTTCCAAGAGTAGCCG CTACCTAGCCACCACGCCCTTTCTTCTGGAAGAGGCTCCCATTGTAAAATCCCATAGCTTTATGTTTTCT CCTTCAAGGAGCTACTACGCCAACTTTGGGGTGCCCGTGAAAACGGCAGAATACACAAGTATTACAGACT GTATCGACACAAGATGTGTCAATGCCCCCCAAGCAATAGCTGACCGAGCCACCTTCCCTGGAGGTCTCGG AGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGAGCGAGAAGCAGAGCTGAGCCATCCTAGCTCTGAC AGTGAGGAAAATGAGGCCAGAGGCCAGAGAGCTGCCAATCCGATATCCTCTCAGGAGGCTGAAAATGCAG ACAGAACCCTATCCAACAACATCACAGTTCCCAAGATAGAGCGCGCCAACAGCTACTCAGCAGAGGAGCC AAACGTGCCATATGCACATACCAGAAAGAGCTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAACACG GCGAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTATCCA TGAGGAGACTGTCTAGAACATCGGCCTTTCATAGCTTTGAAAGCAAGCACAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001035240
Insert Size	5166 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001035240.2, NP_001030317.1
RefSeq Size	6742 bp
RefSeq ORF	5166 bp
Locus ID	226025

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