Cdc42bpb (NM_183016) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MRCKb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224860 representing NM_183016 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCAAGGTGCGGCTCAAGAAGCTGGAGCAGCTGCTCCTGGACGGGCCGTGGCGCAACGATAGCG CCCTGAGCGTGGAGACGCTGCTGGACGTGCTGGTGTGCCTTTACACCGAGTGCAGCCACTCGGCGCTGCG CCGCGACAAGTATGTGGCGGAGTTCCTCGAGTGGGCCAAGCCTTTCACCCAGCTCGTGAAGGACATGCAG CTTCATCGAGAAGACTTCGAGATCATCAAAGTGATCGGGAGAGGAGCCTTTGGTGAGGTTGCTGTTGTCA AAATGAAGAACACTGAACGAATTTATGCAATGAAAATTCTCAACAAATGGGAAATGCTAAAGAGAGCAGA GACAGCTTGCTTTCGAGAAGAGCGTGACGTGCTGGTGAACGGCGACTGCCAGTGGATCACGGCTCTGCAC TATGCCTTTCAGGATGAGAACTACCTGTACTTGGTCATGGATTACTATGTAGGTGGTGATCTGCTGACCC TGCTGAGTAAGTTTGAAGACAAGCTTCCGGAAGACATGGCGAGGTTCTACATTGGCGAGATGGTGTTGGC CATTGACTCGATCCACCAGCTCCACTATGTGCACAGAGACATCAAGCCCGACAACGTCCTTCTAGATGTG AACGGTCACATCCGCCTGGCTGACTTTGGCTCGTGCTTGAAGATGAACGATGATGGCACTGTTCAGTCTT CCGTGGCCGTGGGCACACCTGACTACATCTCACCAGAGATCCTGCAGGCCATGGAGGATGGCATGGGCAA ATACGGGCCCGAGTGTGACTGGTGGTCGCTGGGCGTCTGCATGTATGAGATGCTATACGGAGAAACCCCG TTCTACGCAGAGTCTCTGGTGGAGACATACGGGAAGATCATGAACCACGAGGAGCGGTTTCAGTTCCCAT CCCATGTCACCGATGTCTCTGAAGAAGCAAAAGACCTTATCCAGAGACTAATATGCAGCAGAGAGCGCCG ACTTGGGCAGAATGGAATAGAAGACTTTAAGAAACACGCATTCTTTGAAGGTCTGAATTGGGAGAATATA CGAAACCTTGAAGCGCCCTACATTCCCGATGTGAGCAGCCCTTCGGACACGTCCAACTTCGATGTGGACG ACGACATGCTGAGAAACATTGAAATCTTACCTCCCGGCTCTCACACGGGCTTCTCGGGACTGCATTTGCC CTTCATCGGTTTCACATTCACGACGGAAAGCTGCTTTTCTGACCGGGGCTCTCTGAAGAGCATGACTCAG TCTAACACGCTAACCAAAGACGAAGATGTGCAGCGGGACTTGGAGAACAGCTTGCAGATCGAAGCGTACG AGCGAAGGATACGGAGGCTGGAGCAGGAGAAGCTGGAGCTCAGCCGGAAGCTGCAAGAATCCACCCAGAC TGTGCAGTCCCTTCACGGTTCCACACGGGCCCTGGGCAACTCAAACCGCGACAAGGAAATCAAGAGGCTG AATGAAGAGCTTGAACGCATGAAGAGTAAAATGGCAGATTCAAACAGGCTCGAACGCCAGCTGGAGGACA CAGTGACACTTCGCCAGGAGCATGAGGACTCCACACACCGGCTGAAGGGCCTCGAGAAGCAGTACCGCCT GGCCCGGCAGGAGAAGGAAGAATTGCACAAGCAATTGGTTGAGGCTTCAGAGCGATTGAAATCCCAGACC AAAGAACTTAAAGACGCGCATCAGCAGCGAAAACGGGCCCTGCAGGAGTTCTCAGAGCTCAATGAGCGCA TGTCGGAGCTCAGGTCGCTGAAGCAGAAGGTGTCCCGTCAGCTCCGGGACAAGGAGGAGGAGATGGAGGT GGCCATGCAGAAGATCGACTCCATGCGGCAGGACCTCCGCAAGTCTGAGAAGTCCAGGAAAGAGCTGGAA GCTCGGCTTGAGGACGCAGCTGCCGAGGCCTCTAAGGAGCGGAAGCTCCGAGAACACAGCGAGAGCTTCT GTAAGCAGATGGAGCGCGAGCTCGAGGCCCTCAAGGTAAAGCAAGGAGGCCGGGGGCCAGGGGCCGCGTC GGAACATCAGCAGGAGATCTCCAAAATCAGGTCGGAGCTCGAGAAGAAAGTCTTGTTCTACGAGGAGGAG CTGGTGCGCCGAGAGGCCTCCCACGTGCTAGAAGTAAAGAACGTGAAGAAGGAAGTCCACGACTCCGAAA GCCACCAGTTGGCCCTGCAGAAAGAGGTCCTGATGCTGAAAGACAAGTTAGAAAAGTCAAAGCGAGAACG GCACAGTGAGATGGAGGAGGCCATAGGCACCGTGAAGGACAAGTACGAACGGGAGAGGGCCATGCTGTTC GATGAGAACAAGAAGTTAACAGCAGAAAACGAAAAGCTTTGTTCCTTTGTGGATAAACTAACAGCCCAAA ACAGACAGCTGGAAGATGAGCTGCAGGATCTGGCATCCAAGAAGGAGTCAGTTGCGCACTGGGAGGCGCA GATCGCAGAGATCATTCAGTGGGTCAGCGATGAGAAAGATGCCAGAGGCTACCTTCAAGCCCTTGCTTCT AAGATGACCGAAGAGCTTGAGACCTTGCGAAGTTCTAGTTTGGGATCCAGGACACTGGATCCACTCTGGA AAGTTCGTCGGAGTCAGAAGCTGGACATGTCTGCACGGCTGGAATTGCAGTCTGCTCTTGAGGCCGAGAT CCGTGCCAAACAGCTCGTTCAGGAGGAGCTGAGGAAGGTCAAAGACAGCAGCCTGGCCTTCGAAAGCAAA CTGAAGGAATCGGAAGCGAAAAACAGGGAATTGTTAGAAGAAATGCAAAGTCTGAGGAAGAGGATGGAAG AGAAGTTTAGAGCGGATACAGGGCTCAAACTTCCAGATTTCCAGGATTCTATCTTTGAGTATTTCAACAC TGCTCCTCTTGCACATGATCTGACTTTTAGAACCAGCTCAGCTAGTGACCAGGAAACACAGGCTTCAAAG ATGGACTTGTCCCCATCAGTGTCTGTAGCCACGAGCACAGAGCAGCAGGAGGATATGGCTCGGCCACAGC AGAGGCCGTCTCCTGTGCCACTGCCCAGCACGCAGGCCCTCGCCATGGCTGGACCGAAGCCCAAAGCCCA CCAGTTCAGCATCAAGTCCTTCCCTAGCCCCACCCAGTGCAGCCACTGCACGTCCTTGATGGTCGGACTG ATCCGACAGGGCTACGCCTGTGAGGTCTGTGCATTTTCCTGCCATGTGTCCTGCAAAGACAGCGCTCCCC AGGTGTGCCCCATACCTCCTGAGCAGTCCAAGAGGCCTCTTGGCGTAGACGTGCAGAGGGGCATAGGCAC GGCCTACAAGGGCTACGTCAAGGTCCCAAAGCCCACAGGCGTGAAGAAAGGATGGCAGAGGGCTTACGCC GTGGTCTGTGACTGCAAACTCTTCCTGTACGACCTGCCAGAAGGGAAGTCGACCCAGCCCGGTGTCGTTG CCAGTCAAGTCTTGGACCTCAGAGATGAGGAGTTTGCTGTGAGTTCAGTCCTGGCCTCAGATGTTATCCA TGCTACACGCCGAGACATTCCGTGCATATTCAGGGTGACGGCCTCTCTCTTAGGTTCGCCTTCTAAGACC AGCTCACTGCTCATCCTGACGGAGAACGAGAATGAAAAGAGGAAGTGGGTAGGGATCCTTGAAGGGCTGC AGGCCATCTTGCATAAGAACCGGCTGAAGAGCCAGGTAGTGCACGTCGCACAGGAGGCCTACGACAGCTC GCTGCCGCTCATCAAGGCCGTCCTGGCTGCTGCTATCGTGGATGGAGACAGGATTGCGGTCGGCCTGGAA GAAGGGCTCTACGTCATTGAGCTCACCCGAGACGTGATCGTCCGCGCTGCTGACTGCAAGAAGGTGTACC AGATCGAGCTGGCGCCCAAGGAGAAGATCGCCATCCTCCTGTGTGGCCGGAACCACCATGTGCACCTCTA CCCCTGGTCCTCCTTCGACGGAGCAGAAGCGAGCAACTTTGACATCAAGCTCCCGGAAACAAAGGGCTGC CAGCTCATAGCGACAGGGACGCTGAGGAAGAGCTCGTCCACCTGCCTGTTTGTCGCTGTGAAGCGACTAA TCCTTTGCTACGAGATCCAGAGAACTAAGCCTTTCCACAGGAAGTTCAGTGAGCTGGTGGCTCCGGGACA CGTGCAGTGGATGGCCGTGTTCAAGGACAGGCTCTGTGTTGGCTACCCCTCTGGGTTCTCTCTGTTGAGC ATCCAGGGGGACGGGCCGCCTCTCGACCTGGTAAATCCCACTGACCCCTCGCTCGCGTTCCTCTCACAGC AGTCTTTCGATGCCCTCTGTGCTGTGGAGCTCAAAAGTGAGGAGTACCTGCTTTGCTTCAGCCACATGGG ACTGTACGTGGACCCTCAAGGTCGGAGGTCACGCATGCAGGAGCTCATGTGGCCTGCGGCTCCTGTCGCC TGTAGTTGCAGCCCCACCCATGTCACAGTGTACAGCGAATACGGGGTGGATGTCTTCGACGTGCGCACCA TGGAGTGGGTTCAGACCATCGGCCTGCGGAGGATAAGACCTCTGAACTCTGATGGCAGCCTCAACCTGCT GGGCTGTGAGCCCCCTCGCCTCATCTACTTCAAAAACAAGTTCTCAGGAACAATCCTCAATGTGCCCGAC ACCTCGGACAACAGCAAGAAGCAGATGCTGAGGACACGGAGCAAACGACGTTTTGTCTTCAAGGTTCCCG AGGAAGAGCGGCTACAGCAGCGGCGAGAGATGCTCAGAGACCCCGAACTGCGATCCAAAATGATATCCAA CCCAACCAACTTCAACCACGTGGCTCACATGGGTCCTGGGGATGGCATGCAGGTGCTCATGGACCTGCCT CTGAGTGCTGCACCCACTGTCCAGGAGGAGAAGCAGGGCCCTACCCCAGCAGGCCTCCCCCGGCAGCCGC CATCCAGGAGCAAGCCCTATGTCTCGTGGCCGTCGTCAGGTGGGTCCGAGCCTGGAGTGCCTGTGCCTCT GAGGAGCATGTCCGACCCCGACCAGGATTTTGACAAAGAGCCTGACTCTGATTCCACCAAACACTCAACT CCATCCAATAGCTCCAACCCTAGCGGCCCCCCAAGCCCCAACTCGCCCCATCGGAGCCAGCTCCCTATGG AAGGCCTGGACCAGCCATCCTGTGACGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_183016
Insert Size	5142 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_183016.2, NP_898837.2
RefSeq Size	6718 bp
RefSeq ORF	5142 bp
Locus ID	217866
UniProt ID	Q7TT50
Gene Summary	Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.