Pbrm1 (NM_001081251) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610016F04Rik; AI507524; BAF180; Pb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224845 representing NM_001081251 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCCAAGAGAAGAAGAGCCACCTCTCCTTCCAGCAGTGTCAGTGGAGACTTTGATGACGGGCACC ATTCTGTGCCTACACCAGGCCCAAGCAGGAAAAGGAGAAGACTGTCCAATCTTCCAACTGTAGATCCTAT TGCTGTGTGCCATGAACTCTATAACACCATCCGAGACTATAAGGATGAACAGGGCAGACTCCTCTGTGAG CTGTTCATTAGGGCTCCAAAGCGGAGAAATCAACCAGACTATTATGAAGTGGTTTCTCAGCCCATTGACT TGATGAAAATCCAACAGAAACTTAAAATGGAAGAGTATGATGATGTTAATCTACTGACTGCTGACTTCCA GCTGCTTTTTAACAATGCAAAGGCCTACTATAAGCCAGATTCCCCTGAGTATAAAGCTGCTTGTAAACTC TGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGCAGACGATGAAGATGATGACG AAGATGGGCAAGACAATCAAGGCACACTGGCTGACGGCTCTTCTCCAGGTTATCTGAAGGAGATCCTGGA GCAGCTTCTTGAAGCCATAGTTGTAGCCACAAATCCATCAGGACGGCTCATCAGTGAACTTTTTCAGAAA CTGCCTTCCAAAGTGCAATATCCAGACTATTATGCAATAATTAAGGAACCTATAGATCTCAAGACCATTG CTCAGAGGATACAGAATGGAAGCTACAAAAGTATACACGCAATGGCCAAAGATATAGATCTTCTAGCAAA AAATGCCAAAACATACAATGAGCCTGGGTCTCAAGTATTCAAGGATGCCAATTCGATTAAAAAAATATTT TATATGAAAAAGGCAGAAATTGAACATCATGAAATGACTAAATCAAGTCTTCGAATAAGGACTGCATCAA ATTTGGCTGCAGCCAGGCTGACAGGTCCTTCGCACAATAAAAGCAGCCTTGGTGAAGAAAGAAACCCCAC TAGCAAGTATTACCGTAATAAAAGAGCAGTCCAAGGGGGTCGCTTGTCAGCAATTACCATGGCACTTCAG TATGGATCAGAGAGTGAAGAGGACGCTGCTTTAGCTGCTGCACGCTATGAAGAAGGGGAATCTGAAGCAG AGAGCATCACTTCCTTCATGGACGTTTCCAACCCCTTTCATCAGCTTTACGACACAGTTAGGAGCTGTAG GAATCACCAAGGGCAGCTCATAGCTGAACCTTTCTTCCATTTGCCTTCAAAGAAAAAATACCCAGATTAT TATCAGCAAATTAAAATGCCCATATCACTTCAACAGATCAGAACAAAGCTAAAGAACCAAGAATATGAAA CTTTAGATCATTTGGAGTGTGATCTGAATTTAATGTTTGAAAATGCCAAACGTTATAACGTTCCCAATTC AGCCATCTATAAGCGAGTTCTAAAACTGCAGCAAGTCATGCAGGCAAAGAAGAAGGAGCTTGCGAGGAGA GATGACATTGAGGACGGAGACAGCATGATCTCCTCAGCCACTTCTGACACTGGTAGTGCCAAAAGGAAAA GGAATACTCATGACAGTGAGATGTTGGGTCTCAGGAGGCTATCCAGTAAAAAGAACATAAGAAAACAGCG AATGAAAATTTTATTCAATGTTGTTCTTGAAGCTCGAGAGCCAGGTTCAGGCAGAAGACTTTGCGATCTA TTTATGGTTAAGCCATCCAAGAAGGACTATCCTGATTATTATAAAATCATCTTAGAGCCAATGGACCTGA AAATAATTGAGCATAACATCCGAAATGACAAATATGCAGGTGAAGAAGGAATGATGGAAGACATGAAACT CATGTTCCGCAATGCCAGGCACTACAATGAGGAGGGCTCCCAGGTATACAATGATGCCCATATCCTGGAG AAGTTACTCAAAGATAAAAGGAAAGAGCTGGGCCCTCTGCCTGATGATGATGACATGGCTTCTCCCAAAC TTAAATTGAGTAGGAAGAGTGGTGTTTCTCCTAAGAAATCAAAGTACATGACTCCAATGCAGCAGAAACT GAATGAAGTGTATGAAGCTGTAAAGAACTATACTGATAAGAGGGGTCGCCGCCTTAGTGCTATATTTCTA AGACTCCCCTCTAGATCAGAGCTGCCTGACTACTACCTGACCATTAAAAAGCCCATGGACATGGAAAAAA TTCGAAGTCACATGATGGCAAACAAGTACCAAGACATAGATTCTATGGTAGAGGACTTTGTCATGATGTT TAATAATGCCTGTACCTACAATGAACCAGAGTCTTTGATCTACAAAGATGCCCTTGTACTGCATAAAGTC CTCCTTGAGACTCGGAGAGACCTGGAGGGAGATGAGGATTCTCATGTCCCTAATGTGACGTTGCTGATTC AAGAGCTCATCCATAACCTTTTTGTGTCAGTCATGAGTCATCAGGATGACGAAGGGAGGTGTTACAGCGA CTCCTTAGCAGAAATTCCTGCTGTGGATCCCAACTCTCCCAATAAACCTCCCCTTACATTTGACATTATC AGGAAAAATGTTGAAAGTAATCGGTATCGGCGACTTGATTTATTTCAGGAGCATATGTTTGAAGTATTGG AACGGGCAAGAAGGATGAACCGGACAGATTCCGAAATATATGAGGATGCTGTAGAACTTCAGCAGTTTTT TATTAGAATTCGTGATGAACTCTGCAAAAATGGAGAGATCCTTCTTTCTCCAGCACTCAGCTATACCACA AAACACTTGCATAACGATGTGGAAAAAGAAAAAAAGGAAAAATTGCCTAAAGAAATAGAGGAAGATAAAC TAAAACGCGAAGAAGAAAAAAGAGAAGCTGAAAAAAGTGAAGATTCCTCAGGTACTACAGGCCTCTCAGG CTTACATCGTACATACAGCCAGGACTGCAGCTTTAAGAACAGCATGTATCATGTCGGAGATTATGTCTAT GTTGAACCTGCGGAGGCCAATCTACAACCACATATAGTGTGTATTGAGAGACTGTGGGAGGATTCAGCTG GTGAAAAATGGTTGTACGGCTGTTGGTTTTATCGGCCAAATGAAACATTCCATTTGGCTACACGAAAATT TCTAGAAAAAGAAGTTTTTAAGAGTGACTACTACAATAAAGTACCTGTTAGTAAAATTCTAGGCAAATGT GTAGTCATGTTTGTCAAGGAATACTTTAAATTATGTCCAGAAAACTTTCGCGATGAGGATGTTTTTGTCT GTGAATCGAGGTATTCTGCCAAAACCAAATCTTTTAAGAAAATTAAACTGTGGACCATGCCCATCAGTTC AGTTAGATTTGTCCCTCGGGATGTGCCTTTGCCTGTGGTCCGAGTGGCCTCTGTGTTTGCAAATGCAGAT AAAGGGGATGATGAGAAGAATACAGACAACTCAGATGACAATAGAGCTGAAGACAATTTTAACTTGGAAA AGGAAAAAGAAGATGTTCCTGTGGAGATGTCCAATGGTGAGCCAGGTTGCCACTACTTTGAGCAGCTTCG GTACAATGACATGTGGCTGAAGGTTGGTGATTGTGTCTTCATCAAATCCCACGGCTTGGTGCGCCCTCGT GTGGGCAGAATTGAGAAAGTATGGGTCCGAGATGGAGCTGCATATTTTTATGGCCCTATCTTCATTCATC CAGAAGAAACAGAACATGAGCCCACAAAAATGTTCTACAAAAAAGAAGTGTTTCTGAGTAATCTGGAAGA GACCTGCCCTATGAGTTGTATTCTGGGGAAATGTGCAGTGCTGTCATTCAAGGACTTCCTCTCCTGCAGG CCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCCGCTATAATGAGAGTGACAAGCAGATGA AGAAGTTCAAGGGTTTGAAGAGGTTTTCACTCTCTGCTAAAGTTGTAGATGATGAAATCTACTACTTCAG AAAACCAATCATTCCTCAGAAGGAACCCTCACCTTTGTTAGAAAAGAAGATACAATTGCTAGAAGCTAAA TTTGCAGAGTTAGAAGGAGGAGATGATGATATTGAGGAGATGGGAGAAGAGGATAGTGAAGTCATTGAAG CTCCATCTCTACCTCAACTGCAGACACCCCTGGCCAATGAGTTGGACCTCATGCCCTATACACCCCCACA GTCTACCCCAAAGTCTGCCAAAGGCAGTGCAAAGAAGGAAAGTTCTAAACGAAAAATCAACATGAGTGGC TACATTTTGTTCAGCAGTGAAATGAGAGCTGTGATTAAAGCCCAGCACCCAGACTACTCTTTTGGGGAGC TCAGCAGACTGGTGGGGACAGAATGGAGAAACCTTGAAACAGCCAAGAAAGCAGAATATGAAGAGCGGGC AGCTAAAGTTGCTGAGCAGCAGGAGAGAGAGCGAGCAGCACAGCAACAGCAGCCGAGTGCTTCTCCCCGA GCAGGCACCCCTGTGGGGGCTCTCATGGGGGTGGTGCCACCACCAACACCAATGGGGATGCTCAATCAGC AGTTGACACCTGTTGCAGGCATGATGGGTGGCTATCCGCCAGGCCTTCCACCTTTGCAGGGCCCAGTTGA TGGCCTTGTTAGCATGGGCAGCATGCAGCCACTTCACCCTGGGGGGCCTCCACCTCACCATCTTCCGCCA GGTGTGCCTGGCCTCCCAGGCATCCCACCACCGGGTGTGATGAATCAAGGAGTAGCCCCCATGGTAGGGA CTCCAGCACCAGGTGGAAGTCCGTATGGACAACAGGTAGGAGTTTTGGGACCTCCAGGGCAGCAGGCACC ACCTCCATATCCTGGTCCTCATCCAGCTGGCCCCCCTGTCATACAGCAGCCAACAACGCCCATGTTTGTG GCTCCCCCACCAAAGACCCAAAGGCTTCTCCACTCAGAGGCCTACCTGAAATACATTGAAGGACTCAGTG CTGAATCCAACAGCATTAGCAAGTGGGACCAAACTTTGGCAGCTCGAAGACGGGATGTCCATTTGTCCAA AGAACAGGAGAGCCGCCTACCTTCTCACTGGCTCAAAAGTAAAGGGGCACACACCACCATGGCAGATGCC CTCTGGCGCCTACGGGATTTAATGCTTCGAGACACTCTCAACATCCGACAGGCATACAACCTAGAAAATG TTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081251
Insert Size	5115 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081251.1, NP_001074720.1
RefSeq Size	8117 bp
RefSeq ORF	5115 bp
Locus ID	66923

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