Col4a6 (NM_053185) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BB116301
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224833 representing NM_053185 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCTGGATTGTGGCTGTTCCTGGTTTCCTTATGCTTGACCAAAGAACTGGCAGAATCGGGACAGA AGATGTCCTATGGAAAGCCATGTGGGAGCCGAGATTGCCATGGAGTCTGTACATGCTTTCCTGAGAAAGG AGCAAGAGGGCACCCTGGACCAATTGGAACTCAAGGTCCAGCCGGTCCGGAAGGGTTTACTGGTCCAACT GGTTTATCAGGTTTGAAAGGAGAAAGAGGCTCCCCAGGCCCTCTGGGACCATATGGATCAAAAGGAGATA AGGGTCCCATTGGAGTTCCTGGCTTTGTTGGCATCAGTGGTATTCCGGGCCACCCTGGACAGCCAGGTCC AAGAGGTCCACCTGGCCCAGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCTGATGGC TACCCTGGGATTTTAGGACCACCTGGGCTGCCTGGTCACAAAGGTGCCAAAGGAGAGCCTGTTCCTTTTC AAGGCAGCATTACAGGAATAAGGGGAGATCCTGGGCTGCCTGGACTAGATGGAATCCCTGGTCCATCAGG ATCCTCCGGCTCTCCTGGAGATGCAGGACCCATAGGACCACCAGGCTTACAAGGTCCCCCAGGCCTCCCC GGACTTCCCGGTCCTGAGGGAAATATGGGGTTAGGTTTCCAAGGAGAAAAAGGAGTCAAGGGGGATGTTG GCCTCCCTGGCCCTGCAGGACCACCTCCATCTACTGGGGAACTAGAATTCATGGGATTCCCCAAAGGAAA GAAAGGTTCCAAGGGTGAGCCAGGGCCTCCAGGTTTCCCAGGAATGAGTGGCCCTCCAGGTGTCCCGGAA TTTGGATCTACTGGAGAAAAAGGAGAAAAGGGGATTCTTGGTTTGCCAGGACCTAGGGGTCCCATGGGTT CAGAAGGAATACAAGGCCATCCAGGGAGACAGGGAAAGAAGGGGACTTCAGGTTTTCCTGGGATTAACGG GTTCCCAGGAATTAAGGGTGAAAAGGGAGACATTGGTGTTCGAGGCCCAGACAGTTTCACTGATGCAGAT GGCGCTGTGATCTCAGGTTATCCTGGAGACCCTGGTGTGCTGGGCCTTCCAGGCCTTAGAGGAGATGAAG GCGTCCAAGGCCAACGTGGCCCTTCTGGAACCCCTGGCCTCCCATCATTAACAGGTCTTCCAGGTGCCCT AGGGCCTCAGGGATTTCCTGGCCTGAAAGGAGACCAAGGAAACTCAGGACGTACCACCTTTGGAGAAGCT GGCCTACCTGGCAGGGTTGGTTTACCAGGTTTACCAGGCCTGCCAGGCCCATCAGGCCCACCTGGTCGCA CATTTGAGACTGGACATCTGTCCAACATAGAGCCTGGGTTCCCTGGTCTCCAAGGAGAACAAGGTCCAAA AGGACATCAAGGCCTCAAAGGAGTAAAAGGAGACTCTGGTTTTTGTGCTTGTGAAGGTGGTGCCCCCAAC ATTGGACCACATGGGGAACCAGGTCTGCCTGGGATACAAGGTCCCATTGGTCTACAGGGTTTTAAGGGGA CTAAAGGAGATCCAGGCTCAAGGGGAGCATCTGGTCCTGCAGGGACGCCAGGGCTATTTGGACCTAGAGG TCAGACTGGCCTCAAAGGAAAGAAAGGAGAACCAACTGTCAGTAGAGGATCAAAAATGTCAGGGGACAAA GGTGACCCTGGTCCTCAGGGTACCCCAGGTTTGGCAGGAACTCCGGGCAAGGATGGAAGACCAGGTTTAC CAGGCCTCCCAGGCATTCAGGGAGATGGTGGGTCTGGCTTCCCAGGTGAAAGAGGGTTACCAGGACTTCC TGGTGAAAAAGGCCATGATGGTCCAATTGGACCACCAGGAATTGGGCTGCCAGGACCTCCTGGGCCCCGT GGACTTCCTGGAGATAAAGGAGTAGATGGGTTACCAGGGCAACAAGGCCTCCGTGGAGCTCAAGGAGTCA CCTTGCCTTGTATCATTCCTGGGTCATATGGTCCATCAGGATTTCCTGGAGCTCCTGGATTCCCAGGCTC TAAGGGAGCTCGGGGCCTCCCTGGAATTCCAGGCAAGCCTGGCACTCACGGAAGCAAAGGAGGGCCTGGG AGTCCAGGGTTAATGCATCTCCCAGGATTGCCAGGGTTTCCTGGAGTTCGTGGGGAGAAGGGATTACCTG GGTTTCCTGGGCTTCCTGGAAAAGATGGCTACCCTGGGAAAGCTGGCAGTCCAGGGTTACCAGGTTTCAA GGGAGCTGCTGGTGACATATTTGGTGCTGAAAATGGTGCTTCGGGGGAGCAAGGCCTACAGGGATTGCCA GGGGACAAAGGATTTCCTGGAGACTCTGGCCTTCCAGGACCCAAGGGTTTGAATGGGAAACCTGGTATGC TAGGCCCCAAGGGTGAGCGGGGCAACCCTGGAACATCAGGACCACCAGGACAGCCAGGACCCTCAGGGTC TACTGACCCATTTGGCATCAAGGGCACATCTGGATTCCCAGGAGCACCAGGCCTTCCAGGCATCTCAGGG CACCCTGGAAAGAAAGGTCAAAGAGGAGACATAGGTCAGCCTGGATCAACTGGAAAAAGAGGTCTACCTG GGATAAAAGGCCTTCCTGGTCCTCAAGGGTCACCTGGCTTCCTGGGGAGCCCAGGTTTGTCAGGCGTCAC TGGGTTGCCGGGCATCGAAGGCCAAAAGGGTGAAAAGGGGTCCTCTGGACCAGTAGGATTTCCTGGGTTA CCTGGTCTTCCTGGTCTTCCTGGAGCTGATGGATTAAAGGGATTTTCTGGGTCATTTGGAAAGGTGGGAC AACCTGGACAGACTGGTACTTCCGGAGAAAAAGGAGACAGAGGGGATCCAGGGCCAGTTGGCATATCTAG TCCAAGACCTCCAATGCTAAACCTTTGGTTCAAAGGAGAAAAAGGCTCTCGAGGTTCTGCTGGATCGGAT GGATTTCCTGGGCCCAGAGGTGACAAAGGAGAGCCTGGCATCCCAGGGTTACCAGGTCCACCTGGAGCTA CTGGACAAACCAATACCATCAAAGGGCTCAATGGGAGGCCAGGTTCTCCTGGATCTATGGGAAGAAGGGG ATTACCTGGCCTGAAGGGGTCTCTTGGGATTGCAGGTTTCCCAGGAATGCCAGGGAAAAGCGGTTCACAG GGGCTCACTGGGACTTCTGGCCTCCTGGGAGCAACTGGTATCCCAGGTTTAAAAGGAGATCAAGGCCAAA CACTTGGCATTTCTGGAAGCCCAGGACCCAAGGGACAGCCTGGAGAATTAGGTTTTAAAGGTGTCAAAGG AAAAGATGGACTAGTTGGTGATAGAGGTTACCCAGGAAACAAAGGTGATGGTGGAAAAGTTGGAATTACT GGAGATCCTGGCTTCCCTGGCTCCCCAGGACTTCAAGGGATTTCAGGTATGAATGGAGACCCAGGCCTCC CAGGTTCCTCTGGCCATCTGGGGTCAATTGGCCGCCCTGGACCCTCAGGCTTAATAGGACCTAAAGGATT CCCTGGAGCTCCTGGTTTACATGGACTGAATGGACTTCCAGGCACCAAGGGCACCCATGGCACTCCAGGA GCCAGTATCACTGGTGTTCCTGGGCCGGCTGGCCTGCCTGGTCCCAAAGGGGAAAGGGGAGTGCCAGGAA TTGTCATCGGAGATCCAGGGAAACAAGGTTCAAAGGGACAAAAAGGTGACCAAGGTTCCCCAGGTCTTCA AGGTCCTGCTGGTACCCCTGGAGCCCCAGGCATCTCCTTGCCCTCAGTCATAGCAGGACAGCCTGGTGAC CCTGGGCGGCCAGGCCTAGATGGAGAACGAGGCCGCCCAGGCCCTCCAGGTCCTCCAGGTCCCCCTGGGC CATCCTCAGATCAAGGCGATCCTGGAGACTCTGGCTTCCCTGGGATTCCAGGCCTTCAAGGGTTCAAGGG AAACCAAGGACTTCCAGGTTTCTCTGGCCTCTCTGGAGAGCTAGGGCTAAAGGGCATGAGAGGGGAACCT GGCCTCATGGGGACTCCTGGCAAGATCGGGCAACCAGGAGACCCAGGATTTCCTGGGATGAAGGGGAAGG CGGGGCCAAGAGGCTTTTCCGGCCCACAGGGAGCTCCAGGACACACACCAATTGCTGAAGCTATGCAGGT TCCTCCAGGACCTTTGGGTCTGCCAGGCATTGATGGTATCCCAGGCCTCATAGGAGACCCTGGGTCTCAA GGCTCTGTGGGTCTGCAAGGTTCCAAAGGCCTACCTGGCATCCCTGGTAAGGATGGCCCCAGTGGGCTTC CAGGCCCATCTGGAATCCTTGGTGATCCTGGTCTCCCTGGACTACAAGGACCTCCAGGATTTGAAGGAGC TCCGGGAAATCAGGGCCCCATTGGGCAGCCTGGGATGCCTGGGCACGGTGTGAGAGTGGGCTACACACTA GTAAAACACAGTCAGTCAGAACAGGTGCCACTATGCCCCACTGGGATGAGCCGGCTCTGGGTGGGTTACA GCTTGCTCTTCGTGGAGGGGCAGGAGAAAGCCCATAACCAGGATCTGGGATTTGCTGGCTCCTGCCTGCC CCGATTCAGCACCATGCCCTTCATATACTGCAACATCAATGAAGTGTGCCACTATGCCAGACGCAATGAT AAATCTTACTGGCTCTCCACTACTGCCCCCATTCCCATGATGCCTGTGGGCCAGACCCAGATTCCCCAGT ATATCAGCCGCTGCTCTGTATGTGAGGCACCCTCACAAGCCATTGCTGTACACAGTCAGGACATCATTGT CCCACAGTGCCCTCTGGGCTGGCACAGCCTCTGGATCGGATACTCCTTCCTCATGCACACTGCTGCTGGG GCTGAGGGTGGAGGCCAGTCCCTAGTCTCCCCTGGCTCGTGTCTTGAGGACTTCCGGGCCACTCCTTTCA TTGAGTGCAGCGGCGCCCGTGGTACATGCCACTACTTTGCCAACAAGTACAGTTTCTGGCTGACAACGGT GGAGGAGAGAGGGCAGTTTCGGGAAGAGCCCGTGTCTGAGACGCTGAAAACTGGGCAGCTTCACACCAGG GTGAGCCGCTGCCAGGTGTGTATGAAAACCCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053185
Insert Size	5076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053185.2, NP_444415.2
RefSeq Size	6648 bp
RefSeq ORF	5076 bp
Locus ID	94216

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