Crybg1 (NM_172393) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI462491; AI463325; Aim1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224830 representing NM_172393 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGAGGTCAAGCGGCCGCCGGTCTGGTCGGCGGAGGAAGTCGCAGAAATCCACCGACTCCCCGG GTGCGGATGCGGTGGCTCCCGAGAGTGCGGCCAAGGACGAGGCGGTGTTCGACGACGAGGTGGCGCCAGA CGCTGCGGCTGAGAACTGCTTGGCGGAAAGGAAAGTGAAATCGGTACAAGCGGTTCCCGACGGAGGCGCA GCCCCGGTCGCCTGCCCGGAGCCAAAGGCCAGCTCCTGCCCCAGAGGGCAACCCCGAGGGGAACCGGACC GCAGCAAGCCCCTGTCGCCCACCAAGCGGAAGGGTCGCAGCCGTGTCCCCGAGGCGGTGCCCAGCTCGCC CGGCAGTGGCTCGCGCGCTCCCGCCAAGGAGTCCCCACCCAAGAGGGCGCCCGCTGCGGCGGACAGCGGC GAGGAGGCGGGCCGCGTCGTCCCGCGCGAGCTCACCGTCAAAAGCAGCTCGTTGCTGCCCGAGATCAAGC CAGAGCACAAGAGGGGTCCTCTGCCCCACCACTTTGATAGCCGGGGCGACGGGGGTCGCAGCAGGGGAAC AGGAGCGTCCGACGCCGACGGCCTGAAGCCCAGGAACCAGTTCGGTGTGGGCAGGTCTACTGTGACCACC AAAGTGACCCTCCCCGCCAGACCCAAACATGTGGAACTAAATCTGAAAACTCCGAAGAATCCTGACAGTT TGGGAAATGAGCACAACTCATTTAGCCAGCCAGTTCATAAGGGGAACACAGCCAACAAAATCTCCTTATT TGAAAACAAGCGGGCTAACAGTAGCCCCCGCCACCCCGACGTCCGTAACACAAGGAACAATCCTCCCTCT AGTAAGACATTTGTTGGGAGGGCGAAGCTGAACTTAGCCAAAAAGGCCAAAGAAATGGAGCAACCCGAAA AGAAAGCTGTGTCAACCAGCCACCAGAATGGCCTGGTGGTCAAAGAGCCATCTGCAGAAGGGAAAGGCAC AGCAGTCCCTGTCCCCAGAGAAGAGATTCGTCCAGCCACCAGAGGAGGGGAAGGAGAGTCCACCAAGGAT CAGGTCCTCCGCCCTGAGCCTGAGCAAAGTGATAAGCTGGATGTACAAACAGACACTGCCTATCCTTCCC ACCCAGGGCCCACTGCTCTGGTTCCTGTGAAGGACTCTGAGCTCTCAGGAGAGGGCCAGCCAGAGGCTGC TGACAGCAAAGGCCCCGTCCTTAACAACACAAGTGATGTTGCACAGGACATCGCTGCCATTTGTGATGCC AGAGAGGCTCCCCCAAAGCCACAGGATGCATTTTCTGACTCACAGCCAGCTTCAGCGGCTGAGTCAGCTA GGGAGTCATCTCTCCCGCCTTCTGCACCCATTTCTGTGGACGCTCCCCAAGATGGCTGTGTTCAAGTTCC CATATGCAGTGTTCCCTGCACTGCTCTAGAAGTGTCAGAAAACCACAATGGGTGTGTTTCGCCAGTCTCT CCTCACACTGAGAAAACTCCCATCTCCAAACCTGGAGTGGGAGAAGCCAGTCCCCCTTCCACTCCAGAGC ACAGCCCAGAAACTATGGGGAAAGACTGTCCCTCCAAAGTCCTCGTCCAGGTCCGGTCCTTTGTGCTTCC CGTGGAGAATCCTCAGGATGTGAGCTCTCAGGTCATCTCAGAGAGCTCTGAAGTTAGAGAGGCACAGCTG CCAAGCTGTCACAGTAATGAGCCTGAAGTGGTGTCTGTCACCACTTGTGTTCCGCAGCACGAGGATGCAC CAGACACATCACCCAAGGACATCCATCACAGAGAAGGACATGTGGCACAGTCTCGACCACACATCACACA GTCAGGACCTGAGAAGGCTCCGCCCATCCAGAGCAGCAGTGGGATGCCCCAGACAGCGGAGTTCATCCTC ACCCACTCTCCCAGCAGCAGAAGTCATGCAGAAGGCCCCCAAAGGCCAGGTCAAGATGCGACCAGCCCAC TTACCCCTACCAGGCTTTTGAACATTTCTGCTGGTAGTGACAATAGTGTCTTGGATTCTTCTTCTGATAT GGAAAAATTCACTGAAATTATTAAAAAGATGGACAGTGCAGTATGTGTGCCCCAGAAAAAGAAGAAGGCC CGGGTCCCAAACTCTCCAGCCCCTCACTTCGCTATGCCTCCCATTCACGAGGACAGTTTGGAAAAGGTGT TTGATCCCAACGTGTTCACCATTGGTTTAGGGAAGAAGAAGGAAAGCCTGGCAGAGGTGTCACCAGCTTT GCACTTGTTGCAGAACCACGACCCCATATCCAAACTGAGAGCCAAGCGTGCATCCACCGAGCAGAGCATC GTCTTCAAGTCCTTGCACACTAACAGTAATGGGAAGAGCGAGCCACAGACCACTTCAGAAGCAAGTGACA AGGAGAACAGGGACATCAACAGCGGCGGCGTTAAGAGGTCACGGCTAGAAAACAGCACGCTTTTCTCAAG CATGCTTTCTTCTCTGCCACAAGACAAAGTCTTTTCTCCCTCCGTGACGTCAGTCAACACAATGAGCACA TCTTTTAGCACTTCTCAGAACAGCTCCGTATCTCAGTCATCAGTGGTACAGCCCAGGAAGGAGGGTGGCC TGCCCTGTGGCTCAGACAAAGAACAGCCACACCTTGCACCCCACAACTCCTTAAAGGTCTTCAATTTCAA TTCATCAAATACATCTCGTCTGGGTTCCAGCCACATGGAAAAGTACCCACAAAAAGAGGGAAGCAAGGAA GATGTGAATTCTCAGGGCAACCTACAATTGCCAGAAAACAACCTTTCAGACTTACCCAAACTGAAGAACA GCGATGGCGTGGAAGGTATTCTGAAGTCAAACCTGCCGAATGTTGGGAGCGTGGACGCGGACTTCCTGGG CCTTTTCAAAGCAAGCCGGTATGACCCAGGCCATTCCTTTTCTGGAATGTCCTTATCAGACTCTATGACT CTTAGAGGAAGTATGCACAGCAAACTCAACCCCCGGCCTGGCAAGGTAGTGATATTCAGTGAGCCCGACG TCTCTGAGGAGTGCATTGAGGTGTTTGGGGACATCCAGGACTGCAGCTCCTGGCGCCTCTCTCCAGTGAT AGTAGTGAAAGTTGTCCGAGGATGTTGGATTTTGTATGAGAAAGCCAATTTTGAAGGGCATTCTCTTGCT TTAGAAGAAGGTGAACTGGAACTCTCTAGTATCTGGGGAACAGAGGAAATGTTGGATGAAGAAGCAGAGT CTGATAAACCTGTAGTGATTGGTTCCATCAGACACGTGGTTCAGGATTACAGAATTAGTCAGATCGACTT GTTTACTGAGCCTGAAGGGTTAGGACTCCTGAACTCGTACTTTGATGACACTGAGGAAATGCAGGGCTTC CCTGTGATGCAGAAGACGTGTTCCATCAAAGTGCACTGGGGCACATGGCTGATCTATGAAGAGCCTGGAT TCCAAGGTGTCCCCTTCATTCTGGAGCCCGGAGAATACCCTGACCTATCCTTCTGGGATACCGAGGAAGC GTACATCGGATCCATGCGGCCTCTGAAAATGGGTGGCCGTAAAGTTGAATTCCCTACAGATCCAAAGGTA GTTATTTATGAAAAGCCTTCCTTTGAAGGAAGGTGTATGGAGCTAGAAACAGAAATGAGTAGTTTTATCA TGGAAGGAGGTGAGACAGAAGAAACAACTGGAAACAACAACTTGCCCTTTACATCCGTGGGGTCTATGAA AGTCCTAAGAGGCGTCTGGGTTGCTTATGAGAAGTGTGGCTTCACTGGCCACCAGTATTTGCTGGAGGAG GGAGAATACAGGGACTGGAACGCCTGGGGAGGCTACAATGGAGAGCTCCAGTCTCTGCGGCCTATATTAA GTGATTTTTCAAATGCGCACATGATAATGTACAGTGAGAAGAACTTTGGCTCCAAAGGTTCCAGTATTGA TGTGTTGGGAATTGTTGCCAATTTGAAGGAGACCGGCTACGGAGTGAAGACACAGTCAATCAATGTACTG AGTGGCGTGTGGGTAGCCTATGAAAACCCGGACTTCACGGGGGAGCAGTATGTCCTGGATAAAGGCTTCT ACACCAGTTTTGAGGATTGGGGAGGCAAAAATTGTAAGATCTCTTCCGTCCAACCCATATGCCTGGATTC TTTCACTGGCCCCAGGAGACGAAATCAGATTCACTTGTTTTCAGAACCACAGTTCCAAGGTCAAAGTCAA AGTTTTGAAGAAACAATAGATCAGATTGACGACTCCTTTCAGACCAAGTCTTGCAGAGTTTTGGGAGGCA GCTGGGTTGCTTATGATGGAGAAAATTTCTCTGGCAATCAGTATGTACTGGAAGAAGGCCACTATCCTTG TCTCTCGGCAATGGGATGCCTGCCTGGAGCAACCCTGAAGTCTCTTCGCTTTATAGATTTTGAATTTTCC GAACCAACAATTATTCTTTTCGAAAGAGAAAACTTCAAAGGAAAAAAGATAGAACTGAACGCAGAAACAG TCAATCTCCGATCCTTGGGGTTCAACACTCAGATCCGCTCTGTGCGGGTCGTTGGTGGCATATGGGTTAC CTATGAATATGGCAATTACAGAGGCCGGCAGTTCCTGCTGTCTCCTGCAGAAGTACCAAACTGGTATGAG TTCAGTGCCTGTCGCCAAATAGGCTCCCTCCGGCCCTTTGCTCAGAAACGAATTTACTTCAGACTTCGAA ACAAAGCAACAGGACTATTTATGTCAACCAACGGGAACTTGGAAGACCTGAAGCTTCTCCGAGTCCAGGT CATGGAAGATGTTGGTGCTGATGATCAGATCTGGATCTATCAAGAAGGGTGCATCAAATGCAGGATAGCA GAAGACTGCTGCCTGACGATCGTGGGCAGCCTCGTCACGTCTGGCTCCAAACTAGGACTGGCCCTCGATC AAAATGTCGACAGTCAGTTCTGGTGCATGAAGTCCGACGGCAGGATCTACAGCAAGATGAAACCAAATTT AGTGTTGGATGTGAAAGGGGGTGCGCAGTATGATCAAAATCACATCATCCTCAACACAGTGAGCACAGAG AAGTTAACACAAGTGTGGGAAGCCATGGTCCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172393
Insert Size	5076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_172393.2, NP_765981.2
RefSeq Size	7416 bp
RefSeq ORF	5076 bp
Locus ID	11630

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