Gcc2 (NM_027375) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610043A03Rik; 2210420P05Rik; 2600014C01Rik; AW112121; mKIAA0336
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224816 representing NM_027375 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCTGCTCCAGATGCCGTAGCTGCAGCACCCTCCGGGACCCCGAAATCCAAGCTAGAAACGT TGCCCAGAGAAGATCTCATCAAGTTTGCCAAGAAGCAAATGATGCTCCTGCAGAAAGCCAAAGCAAGGTG TACAGAATTAGACAAAGAAGTTGAAGAACTCAAATCAAAACCTGTTGATGGAGGAACTGATGATATTATT AAGGTACTAACTGAACGCTTGGATGCTCTTCTTCTGGAAAAAGCAGAGACTGAACAACAGTGTCTATGTC TGAAAAAAGAAAATGTTAAAATGAAGCAAGAGGTTGAGGATTCTGTAACTAAACTGGAAGAGACACACAA AGAGTTTGAACAATCACATAGAAACTACGTAAAAGAAATTGAAAGCTGTAAAAATGAGCTGATGGCAGTG CACTCAGAGCACAGCAAAGAAACGGCCATCTTACAAAAAGAACTGGAAGAAGCTGTCCATAAGCAAGTAG AGCTGAGAGAGCAGCTTAAGTCTCAGAGTGACTCTGAAGATAACGTGAGAAAACTGCAGGAAGAGATTCA AAACATCACAGCAGCATTTGAGGAACAGATTTCATGTCTAGAGAAGAAATTAGAAGCTACCAGTGATGAA AAACAACAAGAGATTATTCATCTCCAGAAAGTCATTGAGGACAAGGCTCAGCACTACCAAAAAGATATTA ATACTTTCCAAGCAGAGATTTTGCAGCTGAGAGCTACACACAAAGAAGAAGTTACTGAGCTGATGTCTCA AATAGAAACATCAGCTAAAGAACACGAGGCAGAAATAAATAAACTAAAAGAGAACAGAGTGACACAGTGT GAGGCAAGTGAGAACATTCCAGAGAAATACCAATGTGAATCGGAAAACTTAAATGAAGTCGCCTCAGATG CAAGCCCGGAGAGTCAGAATTGCTCTGTGGCCTTACAGGAAGATCCTTCTGCGGAACAAACAGTATGTGA TAAAGTCAGACAATTGGAAGATTCATTAAAAGAACTGGAATCTCAACATAGTATCTTAAAAGATGAAGTA ACTTATATGAATAATCTCAAGTTAAAACTTGAAATGGATGCCCAGCATATAAAGGATGAGTTTTTCCATG AACGAGAAGACTTAGAGTTTAAAATTAATGAACTGTTGCTGGCTAAAGAAGAACAGGGCTATGTAGTAGA AAAATTAAAATATGAGCGAGAAGATTTAAATAGGCAACTTTGCTGTGCTGTGGAACAACATAACAAAGAA ATACAGCGTCTTCAGGAACACCATCAGAAAGAAGTCTCTGAACTAAGTGAGACATTTATATCAGGTTCAG AAAAAGAAAAGTTAGCATTGATGTTTGAAATACAAGGTCTTAAGGAACAGTGTGAAAATCTACAACATGA AAAGCAAGAGGTAGTTCTAAACTATGAGAGTTTACGAGAGATGATGGAAATTTTACAAACAGAACTTGGA GAATCTGCTGGAAAAATAAGTCAAGAGTTTGAAACAATGAAGCAACAGCAAGCATCTGATGTTCATGAGC TTCAGCAGAAGCTAAGAAGTGCTTTTAATGAAAAAGATGCTCTTCTTGAAACTGTGAATCGCCTCCAAGG AGAAAATGAAAAATTATTATCTCAACAAGAATTAGTACCAGAACTTGAAAGTACCATAAAGAACCTTCAG GCAGATAATAGCATGTACTTAGCCAGTCTTGGTCAAAAAGATACCATGTTACAAGAATTAGAAGCAAAGA TAAGTTCTCTTGCTAAAGAAAAAGATGACTTTATAAGTAAAATCAAAACTTCCCATGAAGAGATGGATGA TCTCCATCAGAAATGGGAAAGGGAACAGAGACTGTCTGTAGAACTCAGGGAAGCAGCAGGGCAAGCTGCC CAGCACAACAGTGAACTGAGACAAAGAGTAAGTGAATTAACAGGGAAACTAGATGAACTAGTAAGAGAAA AGAGTCAAAATGACCAAAGCATTACGGTTCAAATGAAAACTATGACGGAAGATCAAGAAGCTCTGTCATC TAAAATCAAGTCTCTTTATGAGGAAAACAATAGACTACATTCAGAAAAGGCCCAGTTGAGCAGGGATTTA GAAGCACTTCAGGCTCAACAAGATTTTGCCCATAAAGAACATGTTGCTGAATTCGAAAAGAAACTCCAGT TAATGGTTGAGGAGCGAGACGATTTAAATAAACTGCTTGAAAATGAGCAAGTTCAGAAGTCATTTGTTAA AACTCAGTTGTATGAGTATCTTAAGCAACTGAGGGCAAGCATTTTAGAAGAAAATGAAGAGGAAGATGTT GTCAAACTTATACAAGCTGTAGGTGAATCTTTAGTGAAAGTAAAGGAAGAAGAACATAACTTAGTCTTTG AATATGATGCAAGGGTATTAGAATTAGAAAATAAGATTAAGTGTCTTCAAGAGGACAGTGCAGTTCAGTG TGAAGAACTAAGGACTTTAGTAAGAGACTCTGAGCAAGAGAAAATTCTCCTAAGAAAAGAATTAGATGCA GTCACATCAGCAAAAGAGGCCCTCCAACTTGATCTTTTAGAAATGAAGAATACTAATGAGAAAGCAAGCC TTGAAAACCAGACTCTTTCAACTCAGGTTGAAGAATTATCTCAGACATTACACAGCAGAAATGAAGTCCA TGATGAAAAGGTTCTTGTAATAGAACATGAAAACCTAAGGCTACTGCTTAAACAGAGGGAATCTGAACTC CAAGATGTGAGAGCAGAATTGATACTGCTAAAGGATTCCTTAGAGAAATCGCCTTCTGTAAAAAATGATC AACTTTCATTGGTAAAAGAGCTGGAAGAAAAAATAGAAAGTCTGGAAAAAGAATCCAAAGACAAGGATGA GAAAATAAGTAAGATAAAACTAGTTGCTGTGAAAGCAAAGAAAGAACTAGATTCTAACAGGAAAGAGGCC CAGACACTACGGGAAGAACTGGAATCCGTGCGGTCAGAAAAGGACCGCCTGTCTGCTTCTATGAAGGAGT TCCTCCAAGGAGCAGAGAGCTATAAGAGTCTTCTGTTAGAATATGATAAGCAGTCAGAACAGCTGGATGT GGAAAAAGAACGTGCTCATAATTTTGAACGTCATATAGAAGACCTTACCAAACAATTAAGAAATTCGACT TGCCAGTATGAAAGACTAACTTCAGACAACGAGGATCTCCTGGCTCGAATTGAGACCCTGCAGGCTAATG CCAAGTTGTTAGAAGCACAGATCTTAGAAGTCCAGAAAGCCAAAGGAGTAGTAGAAAAAGAACTGGACGC TGAAAAACTTCAGAAAGAGCAGAAAATTAAGGAACATGTCAGTACTGTAAATGAGCTCGAAGAACTCCAA CTCCAATTTCAAAAGGAAAAGAAGCAGCTTCAGAAAACAATGCAAGAATTAGAGCTGGTTAAAAAGGATG CCCAACAGACCACATTGATGAATATGGAAATAGCAGATTACGAACGTTTGATGAAAGAACTAAATCAAAA GTTAACAAATAAAAACAGTACAATAGAAGATTTGGAGCAAGAAATGAAAATTCAGAAAGAGAAACAAGAA ACCCTACAAGAAGAAATAACTTCACTGCAGTCGTCAGTACAGCACTATGAAGAAAAGAACACTAAAATCA AGCAGCTGCTTGTGAAAACCAAAAAGGAGCTGGCAGATGCAAAGCAAGCGGAAACCGATCACTTACTACT CCAGGCATCTTTAAAGGGTGAGCTGGAAGCAAGCCAGCAGCAAGTAGAAGTCTATAAAATTCAGCTGGCT GAGATGACATCTGAGAAACACAAAATCCATGAGCATCTGAAGACTTCTGCGGAACAGCACCAGCGCACAC TGAGTGCATACCAGCAGAGGGTGGTGGCCCTGCAGGAGGAGAGTCGCGCTGCCAAGGCAGAACAAGCTGC TGTCACCTCTGAATTTGAGAGCTACAAAGTCCGAGTTCATAACGTTCTAAAGCAACAGAAAAATAAATCT GTGTCTCAGGTTGAAACTGAGGGAGCTAAACAAGAAAGGGAACACCTGGAAATGCTGATTGACCAACTGA AAATCAAGTTACAGGACAGCCAAAATAGCTTACAAATCAGTGTATCGGAGTACCAGACCCTGCAGGCTGA GCACGACACTCTGCTAGAGAGGCACAACCGGATGCTGCAGGAAACTGTGACCAAAGAGGCAGAACTTCGG GAAAAGCTATGTTCAGTTCAATCTGAGAACACCATGATGAAGTCTGAGCACTCGCAGACAATGTGTCAGC TAACATCCCAGAATGAAGCACTCCGTACCAGCTTCCGAGACCAAGTTCGACACTTGCAGGATGAGCACAG AAAGACAGTGGAGACTCTGCAGCACCAGCTCTCCAAGCTGGAAGCTCAGCTCTTCCAGCTCAAGAGTGAG CCATCCACAAGAAGCCCTGCTTCTTCCCATCAACCTTCGAAAAGCCTCCGAGAAAGAAGAACCACAGACC TCCCCCTTCTGGACATGCACACTGTGGCCCGCGAGGAGGGGGAGGGCATGGAGACAACTGACAGCGAGTC TGTGTCGTCGGCAGGCACACACATTCAGTCCTTAGAGCAGCTCCTTAGCTCTCCTGACACCAAGCTTGAA CGCCTTGCAGAGACCTCCTTGTGGCATAATGAATTTACCAAAGAAGAATTAGCTGAGAAGCTCAGCTCCA CCACGAAGAGTGCAGATCACTTAAACGGCCTGCTTCGGGAGACAGAGGCTACCAACGCTATCCTTATGGA GCAAATTAAGCTTCTCAAGAGTGAAATAAGAAGACTGGAAAGAAACCAAGAGCGTGAGAAGTCTGTGGCT AACCTGGAGTACTTGAAGAATGTCTTGCTGCGGTTCATTTTCCTGAAGCCGGGTAGTGAAAGAGAGAGGC TTCTGCCTGTGATAGACACCATGCTGCAGCTCAGCCCTGAGGAAAAGGGAAAGCTTGCCACAGTTGCTCA AGGTGAGGAAGAAAGTGCTTCTCGTTCCTCTGGATGGGCGTCCTATCTGCATAGCTGGTCTGGACTGCGA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027375
Insert Size	5043 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027375.2, NP_081651.2
RefSeq Size	6528 bp
RefSeq ORF	5043 bp
Locus ID	70297

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