Pcdh15 (NM_001142746) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224811 representing NM_001142746 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGACTGGCAATACGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC TATCGTGGCCATTGATGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACT GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACC CCGTTAAACAGATGCTTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACA CTCCATTGTGGTGCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATC GTGGTGCGAGATCGGAATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGC TCACTCCAGTTGGCACCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGG CCCTAATGGACAGATAGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAA ATTCCACTCATGCTGACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACT ATGTCATCATCCAAGCAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCAC AGTAGATGTTCTAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGT GACTGTCGTCCACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT TGGTGACACCACCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT CCTCTACTCCATCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCA GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGC AGGACAATGGCCACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAG TCCATACTTCACAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCT GAGAGCCTAAACTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATC CAGAGCTCCACCTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTA CCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGAT GGCGTGCAAGAAAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCT TCCCTGAAATCTCCTATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGAC AGCGGTAGATGCTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGAC TTCGTGATCAACAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGT ATGCGCTCACAGTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGGCACTCCATCTGCACAGTGTA CATCGAGGTGCTTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAGCTGATGTACAGTCTGGAAGTC AGCGAGGCCATGAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTGATCGAGAGGGAGATCCAATCA CATATGCCATCGAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGAAACCACAGGGATTCTCAGCCT AGGGAAGGCTCTAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCG GATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATC CCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAAC AGACCCAGATGCTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATC ACATCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTG TCGTGGCAACAGATGGAGCAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACAT TGATGATAACAGTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGG ACCTCCTTTCTTCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCC CAGAAGTGAAACACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTA TGAGGCCTTTCCGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATG CCACCTGGTATAGCAACAGTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAAC GCATCTACAAGGGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGA TGCGGACCCACCTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCA GCCAGTATTTTTGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTA CTACGATTTTCAAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGT GAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTA AGTGAGCTTGCGGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGT ACTCCATTGTGGCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAA TTCACCATTGGATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTC CTTGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGATTCAGGATGAAAACG ATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCAAGGATGTTCGCATC TGTGCTCAGAGTGAAGGCCACCGACAGGGACACGGGTAATTACAGTGCCATGGCCTACCGGCTCATCATA CCGCCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCATCAAGACAGCCATGC TCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGACTACGGGAAGGGGTT GAGCGGGAAAGCAGACGTACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCATTGTCTCCAATGTG CCCCCTACACTAGTGGAAAAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTACGTTCAGGAGCAAA TTCCTGGTGCCAAGGTTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCCTACTCCCTAGAAGA CTATAGCAAGTGCGACCTGACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCATCGACAGAAATGAG CTTTTTAAGTTCCTGGACGGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTATTACGGGGAAGGAG GGCGCATTCTGGAGATTCGGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGAGAAAGCTTGGGGTA CACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAGCCATCTTGGTCGTC TTAGTAAGCTACCGACAGTTTAAAGTACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTCTGCTA TGCCTGCAGCCAAGCCTGCAGCTCCTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCACCACC ACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCAATGCATAAGTATGAGATGCCCCAGTATGGA AGTCGCCGTCGACTGCTGCCACCTGCTGGACAGGAGGAATACGGCGAAGTCATTGGTGAAGCTGAAGAGG AATATGAAGAAGAAGAGTGGGCAAGAAAAAGAATGATCAAGTTGGTGGTCGATCGGGAGTATGAGAGCAG CTCACCTGGGGAAGACAGCGCTCCTGAGTCGCAGAGAAGCAGAACTCACAAGCCCAGTGGCCGCAGCAAT GTCAACGGCAACATCTACATTGCGCAGAATGGTTCCGTGGTGAGAACACGCCGTGCCTGCGTCGCTGATA ACTTGAAGGTGCCCTCCCCTGGGTTGCTTGGGAGGCATCTGAAGAAATTAGACACATTGGCAGGGACACG TGAAGAGAATGTGCCCCTGAACACACTGTTCAAGGGGCCATTTTCCACAGAGAAAGCGAAAAGAACCCCA ACTCTGGTCACGTTTGCCCCGTGCCCCGTGGTGGCTGAGCACTCGGCAGTGAAGCCATCAGGGACCAGGC TGAAACACACAGCTGAGCAGGAGTCCATGGTAGACAGTAGGCTCTCCAGAGAGTCGATGGAATTCCACGG TGACAGCGCGCCATCAGATGAGGAGGAGCTCTGGATGGGTCCGTGGAACAGCCTCCACATACCAATGACA AAACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001142746
Insert Size	5049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001142746.1, NP_001136218.1
RefSeq Size	9150 bp
RefSeq ORF	5049 bp
Locus ID	11994
UniProt ID	Q99PJ1
Gene Summary	Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (K) has a distinct 3' splice pattern, compared to variant A. The resulting isoform (CD3-1), also known as protocadherin-15-CD3 isoform 1, has a distinct and longer C-terminus, compared to isoform CD1-1.

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