Ltbp4 (NM_175641) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310046A13Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224801 representing NM_175641. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGCCGGCCTGGCCTCGGCGGCCCCTGCCCCCTCTTGCTGCTGCTGCTGCTCCCAGCCGCCACATCT GCTAGCGGCTCCAGTCCCAGCCCCAGCCCCAGCCCCATCGAGAAAGCGGTGGTCCCGAGCCACCAGGCC GGTGTTGCTGCCTGTCACTGCTGCCTAGACCAGACACCTAAGAGTAGCCGCTGCACCCGAGCTTCCTGC AGGGTCCGGAACTGCCCACCTGCCAAGTGCACAGGTCTAGAGGGGTGCCTGACCCCAACACCTTCGGTG CCCAGCCCCAGCCGCAGTCCCGTGGAGAAGAGTCAGGTATCCCTCAACTGGCAGCCACTGACTTTGCAG GAGGCCCGAGCTCTGCTGAGGCAAAGGCGGCCCCGAGGGCCGTGGGCCCGGGCATTGCTGAAAAGAAGG CCCCCACATCGCGCCCCTGCCGGCCAGGCCCGGGTCCTATGTCCCTTGATCTGTCACAACGGTGGTGTG TGTGTGAAGCCTGATCGCTGCCTTTGCCCCCCGGACTTCGCTGGCAAATTCTGCCAATTGCATTCTTCG GGGGCCCGGCCCCCGGCCCCAGCCATGCCGGGCCTTACCCGCTCCGTTTATACAATGCCACTAGCCAAC CACCGCGATGATGAACACGGAGTAGCATCGATGGTGAGCGTGCACGTGGAGCACCCTCAGGAGGCATCA GTGGTGGTGCACCAAGTGGAACGTGTGTCGGGCCCCTGGGAGGAGGCGAACCCTGAAGCCCTAGCCAGG GCTGAAGCGGCGGCACGGGCCGAGGCGGCGGCGCCCTACACAGTGCTGGCACAGAGCGCGCCACGCGAG GACGGCTACTCAGACGCCTCCGGCTTCGGTTACTGTTTTAGAGAACTGCGCGGAAGCGAATGTGCATCG CCGCTGCCAGGGCTCCGGACGCAGGAAGTCTGCTGCCGAGGAGAGGGCTTGGCCTGGGGTGTTCATGAT TGTCACCCGTGCGCGGAACACCTGAGGAACTCCAACCAAGTGAGTGGCCCAAATGGACCTTGTCCACCC GGCTTCGAGAGAGTTAATGGGTCCTGTGTAGATGTGGATGAGTGCGCCACCGGTGGGCGTTGCCAACAC GGGGAGTGTGCCAACACTCGTGGCGGCTACACGTGTGTATGTCCGGACGGTTTCCTCCTGGACTCTTCC CGCAGCAGCTGCATCTCCCAACACGTGATCTCCGAGGCAAAGGGGCCCTGCTACCGAGTGCTGCATGAT GGCGGATGCTCGCTGCCCATTCTTCGAAATATCACCAAACAGATCTGCTGCTGTAGCCGTGTGGGCAAG GCGTGGGGCCGAGGCTGCCAGCTCTGTCCACCTTATGGTTCAGAGGGTTTTCGGGAGATCTGTCCAGCT GGCCCTGGCTACCACTATTCAGCTTCTGACCTCCGATACAACACCAGACCCCTAAACCAGGATCCACCT CGAGTGACCTTCAATCAGCCACGTGTTCCACCAGCCACCCCTCGGCCACCTACAGGCTTTCTGCCTACT CGTCGACCTGAGCCCCGCCCTGACCCTGGGCCACAGCCTGAACCCCGGCCTCGGCCTGAACCCCGGCCT CGGCCTGAATCCCGACCTCGGCCTGAACCCCGGCCTCGGCCTGAACCCCGGCCTCAGCCTGAATCCCAG CCTAGGCCTGAATCCCGGCCTCGGCCTGAATCCCAGCCTTGGCCTGAATTTCCCCTGCCCAGCATCCCT GCTTGGACCGGTCCAGAGATTCCTGAATCAGGTCCCTCTTCCAGCATGTGTCAGCGAAATCCCCAGGTT TGTGGTCCTGGACGCTGCGTTCCTAGGCCAAGCGGCTACACTTGTGCGTGCGACCCAGGTTTCCGGCTC GGCCCCCAGGGCACTCGCTGCATTGACATAGATGAATGTCGCCGCGTCCCTACACCCTGTGCTCCTGGG CGTTGCGAGAATACACCAGGCAGCTTTCGCTGCGTGTGCGGCACGGGCTTCCAAGCAGGCCCGCGGGCT ACAGAGTGCCTGGATGTGGACGAGTGCCGCCGCGTGCCGCCGCCGTGTGACCGCGGGCGCTGCGAGAAC ACGCCAGGCAGTTTCCTATGTGTGTGTCCCGCCGGGTACCAGGCAGCACCGCACGGAGCCAGCTGCCAG GATGTGGATGAATGCACCCAGAGCCCAGGCCTCTGTGGTCGCGGGGTCTGCGAAAACCTGCCCGGCTCT TTCCGCTGTGTTTGCCCGGCTGGCTTCCGGGGCTCGGCGTGTGAAGAAGATGTGGATGAATGTGCCCAG CAGCCTCCGCCTTGCGGGCCAGGCCGCTGCGACAACACCGCGGGTTCGTTCCACTGTGCCTGCCCAGCT GGCTTCCGATCCCGAGGACCAGGAGCCCCCTGCCAAGATGTGGATGAATGTTCCCGGAGCCCCTCGCCT TGTGCCTATGGCCGCTGTGAGAACACGGAAGGCAGCTTCAAGTGTGTCTGCCCAACAGGCTTCCAACCC AACGCTGCGGGCTCCGAGTGCGAGGATGTGGATGAGTGTGAGAACCGCTTGGCGTGTCCAGGGCAGGAG TGTGTGAACTCACCGGGCTCCTTCCAGTGCCGGGCCTGCCCTGTTGGGCACCACCTGCACCGTGGCAGA TGTACTGATGTGGATGAATGCAGTTCGGGCACCCCTTGTGGTCTCCATGGCCAGTGCACAAATACTAAA GGCTCCTTCCACTGCAGCTGCTCGACAGGTTACCGGGCGCCATCTGGTCAACCTGGGCCTTGTGCAGAC ATAAATGAGTGTCTGGAAGGCGACTTCTGCTTCCCCCACGGAGAGTGCCTCAACACTGATGGCTCCTTT ACCTGTACCTGTGCCCCTGGCTACCGGCCTGGACCTCGTGGAGCATCTTGTCTGGACGTGGACGAGTGC AGCGAAGAGGATCTTTGCCAGAGCGGCATCTGTACCAACACTGACGGCTCCTTCGAGTGCATCTGTCCT CCTGGACACCGCGCTGGCCCTGACCTTGCCTCCTGCCTTGACATTGACGAGTGTCGTGAACGGGGACCT GCCCTGTGCGGGTCTCAGCGCTGTGAAAATTCTCCTGGTTCCTACCGCTGTGTGCGGGACTGCGATCCA GGTTACCACCCCGGCCCTGAGGGCACCTGTGATGACATTGATGAGTGCCGAGAATACGGCTCTGCGATT TGTGGTGCCCAGCGCTGTGAGAACACCCCGGGCTCCTACCGCTGCACACCAGCCTGCGACCCTGGCTAT CAGCCTACACCAGGGGGCGGGTGCCAAGATGTGGATGAATGCCGGAACCGGTCTTTCTGCGGCGCCCAT GCTATGTGCCAGAATCTGCCTGGGTCCTTTCAGTGCGTCTGTGACCAAGGCTACGAAGGGGCACGGGAC GGACGTCACTGCGTGGATGTGAATGAGTGTGAGACACTACAGGGCGTGTGTGGCTCCGCGCTGTGTGAA AATGTTGAAGGCTCCTTCCTGTGTGTCTGCCCCAATAGCCCTGAGGAATTTGATCCCATGACTGGACGC TGTGTTCCCCCACGGGCTCCTGCAGGCACGTTCCCTGGCTCCCAGCCCCAAGCACCTGCCAGCCCCAGT CTTCCAGCCAGGCCACCAGCCCCTCCCCCACCTCGAAGACCCAGCCCTCCCAGGCAGGGTCCCGTGAGC AGTGGACGTCGCGAGTGCTACTTTGATACAGCCGCTCCAGATGCGTGTGACAATATCTTGGCCCGAAAC GTGACGTGGCAGGAGTGCTGCTGTACTGTGGGAGAAGGCTGGGGCAGCGGCTGCCGCATCCAACAGTGC CCGGGCACCGAGACAGCTGAATACCAGTCATTGTGTCCTCACGGCCGGGGCTACCTGGTGCCCAGTGGA GACCTGAGTGCCCGGAGAGATGTGGACGAATGCCAACTCTTCCAGGACCAGGTGTGCAAGAGCGGAGTG TGTGTGAACACGGCCCCAGGCTACTCTTGCTATTGCAGCAACGGGTTCTACTATCACGCACACAGGCTG GAGTGCGTTGATAATGACGAATGTGCGGACGAGGAGCCTGCTTGTGAAGGTGGCCGCTGCGTCAACACG GTGGGCTCTTACCACTGTACCTGCGAGCCCCCACTGGTGCTGGACGGCTCCCGGCGACGCTGCGTCTCC AACGAGAGCCAGAGCCTGGATGACAATCTAGGAGTGTGCTGGCAGGAAGTGGGACCTGATCTCGTCTGC AGTCGCCCACGACTAGACCGGCAGGCCACCTACACCGAGTGCTGCTGTCTCTATGGTGAAGCCTGGGGT ATGGACTGCGCTCTCTGTCCTGCCCAGGACTCAGATGACTTTGAGGCCCTTTGCAATGTGCTGCGTCCA CCTGCCTATGGCCCGCCGCGCCCTGGTGGCTTTGGAATTCCCTATGAATATGGCCCAGATATAGGCCCA CCTTATCAGAGTCTCCCCTATGGGCCAGATCTGTATCCACCGCCAGTGCTACCCTACGACCCCTACCCA CCCCCACCTGGACCCTTTGCTCGCCGGGAGGCCCCTTATGGCGCCCCACCCTTCGACATGCCGGACTTT GAGGATGATGGTGGCCCCTATGGCGAGTCTGAGACTCCTGACCCACCTAGCCGAGGCACTGGTTGGCCT TATCGGTCCAGAGACACCCGTGGCTCCTTCCCAGAGCCTGAGGAATCCTCTGAGCGTGGAAGCTATACA GGAGCCTTGTCTGAGCCCTATGAGGGCCTAGAGGCCGAAGAGTGTGGTATCCTGGATGGCTGCCCTCAC GGCCGCTGTGTGCGCGTTCCAGAAGGCTTCACCTGCGATTGTTTCGATGGCTACCGCCTGGACATCACC CGCATGTCCTGTGTCGACGTCAACGAGTGTGATGAGGCAGAGGCAACCTCCCCACTCTGCGTCAATGCG CGCTGTGTCAACACTGACGGCTCCTTCCGCTGTATCTGTCGTCCAGGATTTGCTCCCACGCATCAGCCA CATCACTGTGCGCCTGCTCGACCCCGGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_175641
Insert Size	5001 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175641.2
RefSeq Size	5322 bp
RefSeq ORF	5001 bp
Locus ID	108075
UniProt ID	Q8K4G1
MW	178.6 kDa
Gene Summary	Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

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