Col11a2 (NM_009926) Mouse Untagged Clone

CAT#: MC224786

Col11a2 (untagged) - Mouse collagen, type XI, alpha 2 (Col11a2), (10ug)



  "NM_009926" in other vectors (4)

CNY 21,280.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224786 representing NM_009926
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTTCTGTTCCTACCTCTGGTGCTGGGTCTGAGCGCTGCCC
CGGGATGGGCAGGTGCTCCCTCTGTGGATGTGCTTCGTGCCCTGAGGTTCCCCTCCCTTCCCGATGGTGT
TCGGAGATCAAAAGGGGTCTGTCCGGGTGATGTGGCTTACCGTGTGGCACGGCCTGCCCAGCTCAGCGCA
CCCACGCGCCAGCTCTTCCCAGGAGGCTTTCCCAAAGACTTCTCTCTGCTGACGGTTGTCCGGACCCGCC
CTGGCCTCCAGGCTCCCCTCTTGACTCTATACAGCGCCCAGGGAGTCCAGCAGCTGGGCTTGGAGCTCGG
CCGCCCTGTCCGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA
GGCCTCAGCCTAGCAGATGGCAAATGGCACCACGTGGCTGTGGCTGTGAAGGGTCAGTCTGTCACTCTCA
TTGTGGACTGTAAGAAGCGAGTTACCCGGCCCCTTCCCAGAAGTGTGCATCCGGTGTTGGACACCCACGG
GGTGGTGATCTTTGGTGCCCACATCCTCGACGATGAAGTCTTTGAAGGCGATGTTCAGGAGCTCCTCGTT
GTCCCAGGCGTCCAAGCTGCCTATCAGTCTTGTGGGCAGAAGGATCTGGAATGTGAGAGAGAACAGAGGG
ACGGCCCTCAGACTCAGAAGCCTCACAGAGCCCAGAGATCTCCAAAGAAGGAACCAGCAAGACTTCATAA
GCCACAGAGCCAGGAGCCCCAGAAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGT
CCCTTGCCATTCCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGAGAGCCTGCAG
TGCTGGAGCCTGGTATGTTTGTAGAGGGACCCCCAGGCCCAGAAGGCCCAGCGGGATTAGCTGGACCCCC
TGGCATCCAGGGGAACCCAGGCCCGGTTGGAGACCCCGGTGAGAGGGGCCCCCCTGGCCGAGCAGGGCTC
CCCGGATCAGATGGACCCCCTGGTCCTCCCGGCACATCTCTGATGCTTCCATTCCGGTTTGGCAGTAGTG
GGGGTGACAAGGGCCCCGTGGTGGCAGCCCAGGAGGCCCAGGCCCAGGCGATTCTGCAGCAGGCACGGCT
GGCACTCCGTGGGCCCCCTGGCCCCATGGGTTACACGGGCCGCCCTGGACCATTGGGTCAGCCTGGGAGC
CCTGGCTTGAAGGGAGAATCTGGAGATCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGCCTCACAGGTC
CTCCTGGCAAGGCTGGACGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGTGGGATGCCGGGAGAACCTGG
CATGAAGGGTGACCGAGGTTTCGACGGACTTCCAGGGCTACCTGGCGAGAAGGGACAAAGGGGTGATACA
GGTGCTCAGGGCCTTCCTGGGCCTCCTGGTGAGGACGGAGAGAGGGGTGATGATGGAGAGATTGGGCCAC
GGGGGCTGCCTGGAGAGTCGGGACCTAGAGGACTCCTTGGCCCTAAAGGCCCGCCTGGTATTCCTGGGCC
GCCGGGAGTCCGAGGCATGGACGGTCCCCACGGCCCCAAAGGGAGCTTGGGACCTCAAGGAGAGCCAGGA
CCTCCTGGACAACAGGGTACTCCTGGGGCCCAGGGCCTCCCCGGACCTCAGGGAGCCATCGGTCCTCATG
GAGAGAAGGGTGCTCGTGGGAAACCAGGCCTCCCTGGCATGCCTGGATCAGATGGACTCCCGGGTCACCC
AGGGAAGGAAGGTCCCCCTGGAACCAAAGGGAACCAGGGCCCGTCCGGACCACAGGGTCCTCTAGGATAC
CCAGGCCCTCGAGGCGTCAAGGGTGTGGATGGAATTCGGGGCCTGAAGGGCCACAAGGGTGAAAAGGGCG
AGGACGGCTTTCCTGGGTTCAAAGGTGACATAGGAGTGAAAGGAGACAGGGGCGAGGTTGGAGTCCCTGG
TTCCAGGGGCGAAGACGGCCCTGAAGGGCCAAAAGGGCGCACTGGACCTACAGGAGACCCTGGACCCACT
GGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGCCAGGGCCCCA
AGGGATCTCTGGGTTTCCCTGGTTTTCCTGGAGCCAGTGGAGAGAAGGGAGCTCGGGGCCTGTCTGGGAA
ATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGGGGTCAGCGGGGACCTCGAGGTGCCACTGGG
AAATCTGGAGCTAAGGGAACATCAGGTGGTGACGGTCCCCACGGGCCACCCGGAGAGAGGGGTCTTCCTG
GACCTCAAGGCCCCAATGGATTTCCTGGCCCCAAAGGCCCTCCGGGCCCTGCAGGGAAGGATGGGCTGCC
GGGACACCCCGGCCAGAGAGGAGAAGTGGGATTCCAAGGAAAGACCGGCCCACCAGGCCCGCCCGGAGTG
GTGGGACCTCAGGGAACAGCTGGAGAAAGTGGTCCCATGGGAGAGAGAGGTCACTCTGGCCCCCCAGGAC
CTCCTGGAGAGCAAGGATTGCCTGGAACATCTGGGAAAGAAGGGACCAAGGGTGACCCTGGTCCTCCTGG
GGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGAGGCTTCCCAGGAGAGCGAGGCCTTCCAGGCACTGCT
GGTGGACCCGGCTTGAAAGGAAATGAAGGTCCAGCTGGCCCTCCTGGCCCTGCAGGCTCTCCTGGCGAGC
GAGGTGCAGCAGGATCAGGGGGCCCCATTGGTCCCCCGGGACGTCCAGGCCCACAAGGTCCCCCTGGAGC
AGCAGGAGAGAAAGGCGTACCGGGCGAGAAAGGCCCTATTGGTCCCACTGGTCGTGATGGGGTGCAGGGC
CCCGTGGGGCTTCCTGGTCCTGCAGGACCCCCAGGCGTGGCTGGAGAGGATGGAGACAAGGGTGAAGTGG
GAGACCCTGGACAGAAGGGAACCAAAGGAAACAAGGGTGAACATGGCCCTCCTGGACCTCCTGGTCCCAT
CGGGCCTGTGGGGCAACCTGGAGCTGCGGGAGCTGATGGTGAGCCTGGAGCTCGGGGACCCCAGGGACAC
TTTGGAGCCAAAGGTGATGAAGGAACAAGAGGGTTCAATGGACCCCCGGGACCCATCGGCCTACAGGGCC
TGCCAGGACCCTCTGGGGAGAAAGGAGAAACAGGAGACGGGGGGCCTATGGGACCCCCTGGCCCTCCAGG
ACCTCGAGGCCCCGCTGGACCCAATGGTGCTGATGGCCCACAAGGTTCCCCTGGAGGTGTTGGAAACTTG
GGTCCCCCTGGAGAAAAGGGTGAACCGGGGGAGTCAGGGTCTCCAGGCGTCCAGGGCGAGCCGGGCGTCA
AGGGACCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGGCGGGAGAGGCTGGACCACCGGGGCC
CAAAGGCCCTACAGGCGACAATGGCCCCAAGGGGAACCCTGGTCCTGTTGGCTTTCCTGGGGACCCTGGC
CCCCCTGGAGAAGCTGGCCCACGGGGCCAGGATGGTGCTAAGGGAGACCGAGGCGAGGATGGCGAGCCAG
GACAACCTGGATCCCCTGGTCCCACCGGGGAGAATGGGCCCCCTGGACCCCTTGGAAAGCGGGGACCTGC
TGGCACTCCTGGTCCAGAAGGACGGCAAGGAGAGAAGGGAGCTAAGGGGGACCCTGGTGCTGTGGGGGCC
CCGGGAAAGACAGGCCCTGTGGGTCCTGCAGGCCTAGCAGGAAAGCCCGGCCCCGATGGTCTTCGGGGGC
TCCCGGGTTCAGTGGGTCAGCAAGGCCGCCCTGGAGCCACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGG
ACCCCCAGGGCTTCCTGGCCTCCGGGGTGATGCTGGAGCCAAGGGGGAAAAGGGTCACCCAGGTCTCATC
GGACTGATTGGGCCGACTGGAGAGCAAGGCGAGAAGGGCGACCGTGGCCTCCCTGGACCTCAGGGCTCAC
CCGGACAGAAGGGAGAGACGGGTATCCCAGGAGCATCTGGCCCCATCGGTCCTGGAGGGCCTCCTGGCCT
GCCTGGACCCTCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGCTGGACCCAAGGGAGAGAAGGGT
GTCCAGGGCCCTCCAGGACACCCGGGCCCCCCGGGAGAGGTGATCCAGCCACTGCCCATCCAGATGCCCA
AGAAGACCCGCCGTTCCGTGGACGGAAGCAAACTGATACAGGATGAGGAGGCTGTGCCCACTGGCGGTGC
TCCGGGCAGTCCTGCGGGGCTGGAGGAGATCTTTGGCTCACTGGACTCTCTGCGGGAGGAGATCGAGCAG
ATGAGGAGGCCGGCGGGGACCCAGGACAGCCCTGCTCGCACCTGCCAGGACTTGAAGCTGTGCCACCCGG
AGCTTCCTGATGGAGAGTACTGGGTTGACCCTAACCAGGGCTGTGCTCGGGATGCCTTCCGGGTGTTCTG
CAACTTCACAGCAGGAGGGGAGACGTGTGTCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGAC
TCCGAGGGCTCCCCAGTGGGCGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCTGCCCACCAGG
ATGTCTCCTACCCTTGCTCTGGAGTATCCCAGGATGGTCCCCTGAAACTCCGAGGGGCCAACGAGGATGA
GCTGAGCCCTGAGACCAGCCCTTATGTCAAGGAGTTCAGAGATGGCTGTCAGACCCAGCAAGGCCGGACG
GTGTTGGAGGTGCGCACGCCTGTACTGGAGCAGCTGCCCGTGCTGGATGCCTCCTTCGCAGACCTGGGGG
CCCCCACAAGACGGGGAGGGGTGCTTCTGGGGCCTGTCTGCTTCATGGGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009926
Insert Size 4953 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009926.2, NP_034056.1
RefSeq Size 6346 bp
RefSeq ORF 4953 bp
Locus ID 12815
UniProt ID Q64739
Gene Summary This gene encodes the alpha-2 subunit of type XI collagen, one of the low abundance fibrillar collagens found in cartilage. The encoded protein, in association with other collagen subunits, forms a heterotrimeric type XI procollagen that may undergo proteolytic processing similar to the alpha-1 subunit. Mice lacking the encoded protein exhibit a mild phenotype similar to nonocular Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED) as well as a nonsyndromic form of deafness called DFNA13. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks two exons in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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