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Alk (NM_007439) Mouse Untagged Clone
CAT＃: MC224763
Alk (untagged) - Mouse anaplastic lymphoma kinase (Alk), (10ug)
CNY 16,648.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD246; Tcrz |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224763 representing NM_007439
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCTGCTGGGTTCCTGTGGCTGCTGCCTCCACTGCTTTTGGCAGCAGCCTCGTACTCCGGAGCTG CAACCGATCAGCGCGCGGGTTCCCCAGCCTCAGGGCCTCCTCTGCAGCCCCGGGAGCCGCTCAGTTATTC GCGCCTGCAGAGGAAGAGTCTGGCAGTGGACTTCGTTGTACCCTCGCTCTTCCGCGTCTATGCCCGAGAC CTGCTGCTACCGCAGCCACGGTCCCCCTCGGAGCCCGAGGCTGGCGGGCTGGAGGCGCGGGGATCACTGG CCCTGGATTGTGAGCCTCTGCTCAGGCTGCTGGGGCCACTGCCTGGAATCTCCTGGGCAGATGGAGCCAG TTCTCCTAGTCCCGAGGCGGGTCCGACGCTGTCCAGGGTGCTGAAGGGAGGCTCGGTGCGCAAGCTCAGG CGTGCCAAACAGCTGGTGCTGGAGCTGGGCGAGGAGACGATTCTTGAAGGCTGTATTGGTCCCCCAGAGG AGGTAGCGGCTGTGGGGATACTCCAGTTCAACCTCAGCGAGCTGTTCAGCTGGTGGATTCTCCACGGCGA AGGGAGGCTGAGGATCCGCCTGATGCCTGAGAAGAAGGCATCGGAAGTGGGCAGGGAGGGAAGGCTATCC AGTGCGATCCGAGCCTCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACGGGACACAGCTCCATGGAGT CACCCTCAGAAACGCCTTCTCCTCCTGGTACCTTCATGTGGAATCTGACCTGGACGATGAAAGACTCCTT CCCTTTCCTTTCCCACCGCAGTCGATATGGTCTGGAGTGCAGCTTTGACTTCCCCTGTGAGCTGGAATAT TCTCCTCCCCTGCACAACCACGGGAATCAGAGCTGGTCCTGGCGCCATGTGCCCTCCGAGGAGGCCTCGA GGATGAACTTGCTGGATGGGCCAGAGGCAGAGCATTCTCAAGAGATGCCCAGAGGCTCCTTCCTCCTCCT GAACACCTCTGCAGATTCCAAGCATACCATTCTGAGCCCATGGATGAGGAGCAGTAGTGATCACTGCACA CTGGCTGTCTCCGTGCACAGACATCTACAGCCTTCGGGGAGATATGTTGCCCAGCTCCTACCCCACAACG AAGCTGGAAGAGAGATTCTTTTGGTGCCCACCCCAGGGAAGCATGGCTGGACAGTGCTGCAGGGGAGAGT CGGGCGCCCAGCAAACCCATTTCGAGTGGCTCTGGAATACATCTCCAGTGGCAACCGGAGCTTGTCGGCG GTGGATTTCTTTGCCCTGAAGAACTGCAGTGAAGGGACATCCCCAGGCTCCAAGATGGCATTGCAGAGTT CCTTCACTTGTTGGAATGGGACCGTCCTCCAGCTCGGGCAAGCCTGTGATTTCCACCAGGACTGTGCCCA AGGAGAAGATGAGGGCCAGCTGTGCAGTAAACTTCCTGCTGGATTTTACTGTAACTTTGAAAATGGCTTC TGTGGCTGGACCCAAAGTCCACTCTCACCCCATATGCCCCGGTGGCAAGTGAGGACCCTAAGAGATGCCC ATTCCCAGGGCCACCAAGGCCGTGCCCTGTTGCTCAGCACCACTGACATCCTCGCTTCTGAAGGTGCAAC AGTGACCAGTGCCACCTTCCCTGCACCAATGAAAAATTCTCCTTGTGAGCTCCGCATGTCCTGGCTCATC CGCGGGGTTTTGAGAGGAAACGTATCTCTGGTGCTGGTGGAGAACAAAACCGGAAAGGAGCAAAGCCGGA CTGTCTGGCATGTCGCCACTGACGAAGGCTTAAGCCTGTGGCAGCATACAGTGCTGTCCCTCCTCGATGT GACTGACAGGTTCTGGCTGCAGATAGTCACATGGTGGGGTCCAGGATCCAGGGCAACCGTGGGATTTGAC AACATTTCCATCAGCCTCGACTGCTACCTTACCATCAGTGGAGAGGAGAAAATGTCCCTGAATTCAGTAC CCAAATCTAGAAATCTGTTTGAGAAAAACCCAAACAAGGAGTCAAAATCCTGGGCAAACATATCAGGACC AACTCCCATCTTCGACCCTACAGTTCACTGGCTGTTCACCACGTGTGGGGCCAGTGGACCTCATGGCCCC ACCCAGGCACAGTGCAACAACGCCTACCAGAATTCCAACTTGAGCGTGGTGGTGGGAAGTGAAGGGCCCT TGAAGGGAGTCCAGATTTGGAAAGTGCCAGCTACTGACACCTACAGTATCTCGGGCTACGGAGCAGCTGG CGGGAAAGGTGGGAAAAACACCATGATGCGGTCCCATGGCGTGTCTGTCCTGGGCATCTTCAATCTGGAG AAAGGTGACACACTCTACATCCTTGTCGGTCAGCAAGGGGAGGATGCCTGTCCCAGGGCAAACCAACTAA TCCAGAAAGTCTGTGTGGGTGAGAACAATGTCATAGAAGAAGAGATCCGAGTGAACAGAAGCGTGCACGA GTGGGCAGGAGGAGGAGGAGGTGGGGGTGGAGCCACCTACGTGTTTAAGATGAAAGATGGCGTGCCTGTA CCCCTGATCATTGCAGCTGGTGGTGGTGGCAGGGCCTATGGGGCCAAGACAGAAACGTTCCACCCAGAGA GACTGGAGAGTAACTCCTCGGTTCTAGGGCTGAACGGCAATTCCGGAGCCGCAGGTGGTGGAGGCGGCTG GAATGATAACACTTCCTTGCTCTGGGCCGGAAAGTCTTTGCTGGAGGGTGCCGCCGGAGGACATTCCTGC CCCCAGGCCATGAAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCT CAGGTGGAGGAGGCGGAGGATATATAGGTGGCAACGCAGCATCAAACAATGACCCCGAAATGGATGGGGA AGATGGGGTTTCCTTCATCAGTCCATTGGGTATCCTGTACACCCCGGCCTTAAAAGTGATGGAGGGCCAC GGGGAAGTGAATATCAAGCATTATCTAAACTGCAGTCACTGCGAGGTAGACGAATGTCACATGGACCCCG AGAGCCACAAAGTCATCTGCTTCTGTGATCATGGGACCGTGCTGGCTGATGATGGTGTCTCCTGCATTGT GTCACCCACCCCGGAGCCCCACCTGCCGCTCTCATTGATCCTCTCCGTCGTGACCTCTGCCCTGGTGGCT GCCCTTGTTCTGGCATTCTCCGGCATCATGATTGTGTACCGTCGGAAGCACCAGGAGTTGCAGGCTATGC AGATGGAACTGCAGAGCCCCGAGTATAAGCTGAGCAAGCTACGGACCTCGACCATCATGACCGACTACAA CCCCAACTACTGCTTCGCTGGCAAGACTTCCTCCATCAGTGACCTGAAAGAAGTGCCACGGAAAAACATC ACACTCATCCGGGGCCTAGGCCATGGCGCATTTGGGGAGGTGTATGAAGGCCAGGTGTCTGGAATGCCCA ATGACCCAAGCCCTCTACAAGTGGCTGTAAAGACGCTGCCAGAAGTGTGTTCAGAACAAGATGAGCTGGA CTTTCTCATGGAAGCTCTGATCATCAGCAAATTCAACCACCAGAATATTGTTCGCTGCATCGGGGTGAGT CTACAAGCCCTGCCCCGCTTCATCCTGCTGGAACTCATGGCTGGCGGAGACCTCAAGTCCTTCCTCAGGG AGACACGCCCTCGCCCGAACCAACCCACCTCCCTGGCCATGCTGGACCTTCTGCATGTGGCTCGGGACAT TGCCTGTGGCTGTCAGTACCTAGAGGAGAATCACTTTATCCACCGGGATATTGCTGCTAGAAACTGTCTG TTGACCTGCCCAGGAGCTGGAAGAATAGCAAAGATTGGAGACTTTGGGATGGCCCGAGATATCTACAGGG CCAGCTACTACCGAAAGGGAGGCTGCGCCATGCTGCCGGTCAAGTGGATGCCCCCTGAAGCCTTCATGGA AGGGATATTTACCTCTAAAACAGACACATGGTCTTTTGGAGTGTTGCTATGGGAAATATTTTCTCTTGGA TATATGCCGTACCCCAGCAAGAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGCGGAGGACGGATGGACC CGCCTAAGAACTGCCCCGGGCCTGTATACCGGATAATGACGCAGTGCTGGCAGCATCAGCCTGAAGACAG ACCCAACTTCGCCATCATTTTGGAGAGGATCGAATACTGCACCCAGGACCCCGATGTGATCAACACAGCT CTGCCCATCGAATACGGTCCAGTAGTAGAAGAGGAGGAGAAAGTGCCCATGCGCCCCAAAGACCCCGAGG GGATGCCACCTTTGCTGGTGTCTCCCCAGCCTGCGAAGCACGAGGAGGCGTCCGCAGCTCCCCAGCCCGC AGCCCTGACGGCACCAGGCCCATCGGTGAAGAAGCCCCCGGGTGCGGGTGCGGGCGCGGGCGCGGGTGCG GGTGCCGGCCCGGTGCCCCGAGGTGCGGCCGATCGGGGCCACGTGAACATGGCTTTCTCTCAGCCCAACC CTCCCCCGGAGCTGCACAAAGGCCCGGGATCCAGAAACAAGCCGACCAGCCTGTGGAACCCCACCTACGG CTCGTGGTTCACCGAGAAGCCTGCCAAAAAGACCCATCCTCCGCCAGGCGCCGAGCCGCAGGCGCGGGCA GGAGCGGCCGAGGGTGGCTGGACCGGGCCGGGCGCGGGGCCCCGCAGAGCCGAGGCAGCGCTGCTGCTAG AGCCATCGGCGCTGAGCGCCACCATGAAGGAGGTGCCGCTGTTCAGGCTGCGCCACTTCCCCTGCGGCAA TGTCAACTATGGTTACCAGCAACAGGGTCTCCCCTTGGAAGCCACAGCCGCGCCAGGGGACACCATGCTG AAAAGCAAGAATAAGGTCACCCAGCCGGGGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007439 |
| Insert Size | 4866 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007439.2, NP_031465.2 |
| RefSeq Size | 5918 bp |
| RefSeq ORF | 4866 bp |
| Locus ID | 11682 |
| UniProt ID | P97793 |
| Gene Summary | Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221949 | Alk (tGFP-tagged) - Mouse anaplastic lymphoma kinase (Alk), (10ug) |
CNY 17,770.00 |
|
| MR221949 | Alk (Myc-DDK-tagged) - Mouse anaplastic lymphoma kinase (Alk) |
CNY 16,632.00 |
|
| MR221949L1 | Lenti ORF clone of Alk (Myc-DDK-tagged) - Mouse anaplastic lymphoma kinase (Alk) |
CNY 18,050.00 |
|
| MR221949L2 | Lenti ORF clone of Alk (mGFP-tagged) - Mouse anaplastic lymphoma kinase (Alk) |
CNY 19,032.00 |
|
| MR221949L3 | Lenti ORF clone of Alk (Myc-DDK-tagged) - Mouse anaplastic lymphoma kinase (Alk) |
CNY 19,032.00 |
|
| MR221949L4 | Lenti ORF clone of Alk (mGFP-tagged) - Mouse anaplastic lymphoma kinase (Alk) |
CNY 18,050.00 |
