Alk (NM_007439) Mouse Untagged Clone

CAT#: MC224763

Alk (untagged) - Mouse anaplastic lymphoma kinase (Alk), (10ug)



  "NM_007439" in other vectors (6)

CNY 16,648.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CD246; Tcrz
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224763 representing NM_007439
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCTGCTGGGTTCCTGTGGCTGCTGCCTCCACTGCTTTTGGCAGCAGCCTCGTACTCCGGAGCTG
CAACCGATCAGCGCGCGGGTTCCCCAGCCTCAGGGCCTCCTCTGCAGCCCCGGGAGCCGCTCAGTTATTC
GCGCCTGCAGAGGAAGAGTCTGGCAGTGGACTTCGTTGTACCCTCGCTCTTCCGCGTCTATGCCCGAGAC
CTGCTGCTACCGCAGCCACGGTCCCCCTCGGAGCCCGAGGCTGGCGGGCTGGAGGCGCGGGGATCACTGG
CCCTGGATTGTGAGCCTCTGCTCAGGCTGCTGGGGCCACTGCCTGGAATCTCCTGGGCAGATGGAGCCAG
TTCTCCTAGTCCCGAGGCGGGTCCGACGCTGTCCAGGGTGCTGAAGGGAGGCTCGGTGCGCAAGCTCAGG
CGTGCCAAACAGCTGGTGCTGGAGCTGGGCGAGGAGACGATTCTTGAAGGCTGTATTGGTCCCCCAGAGG
AGGTAGCGGCTGTGGGGATACTCCAGTTCAACCTCAGCGAGCTGTTCAGCTGGTGGATTCTCCACGGCGA
AGGGAGGCTGAGGATCCGCCTGATGCCTGAGAAGAAGGCATCGGAAGTGGGCAGGGAGGGAAGGCTATCC
AGTGCGATCCGAGCCTCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACGGGACACAGCTCCATGGAGT
CACCCTCAGAAACGCCTTCTCCTCCTGGTACCTTCATGTGGAATCTGACCTGGACGATGAAAGACTCCTT
CCCTTTCCTTTCCCACCGCAGTCGATATGGTCTGGAGTGCAGCTTTGACTTCCCCTGTGAGCTGGAATAT
TCTCCTCCCCTGCACAACCACGGGAATCAGAGCTGGTCCTGGCGCCATGTGCCCTCCGAGGAGGCCTCGA
GGATGAACTTGCTGGATGGGCCAGAGGCAGAGCATTCTCAAGAGATGCCCAGAGGCTCCTTCCTCCTCCT
GAACACCTCTGCAGATTCCAAGCATACCATTCTGAGCCCATGGATGAGGAGCAGTAGTGATCACTGCACA
CTGGCTGTCTCCGTGCACAGACATCTACAGCCTTCGGGGAGATATGTTGCCCAGCTCCTACCCCACAACG
AAGCTGGAAGAGAGATTCTTTTGGTGCCCACCCCAGGGAAGCATGGCTGGACAGTGCTGCAGGGGAGAGT
CGGGCGCCCAGCAAACCCATTTCGAGTGGCTCTGGAATACATCTCCAGTGGCAACCGGAGCTTGTCGGCG
GTGGATTTCTTTGCCCTGAAGAACTGCAGTGAAGGGACATCCCCAGGCTCCAAGATGGCATTGCAGAGTT
CCTTCACTTGTTGGAATGGGACCGTCCTCCAGCTCGGGCAAGCCTGTGATTTCCACCAGGACTGTGCCCA
AGGAGAAGATGAGGGCCAGCTGTGCAGTAAACTTCCTGCTGGATTTTACTGTAACTTTGAAAATGGCTTC
TGTGGCTGGACCCAAAGTCCACTCTCACCCCATATGCCCCGGTGGCAAGTGAGGACCCTAAGAGATGCCC
ATTCCCAGGGCCACCAAGGCCGTGCCCTGTTGCTCAGCACCACTGACATCCTCGCTTCTGAAGGTGCAAC
AGTGACCAGTGCCACCTTCCCTGCACCAATGAAAAATTCTCCTTGTGAGCTCCGCATGTCCTGGCTCATC
CGCGGGGTTTTGAGAGGAAACGTATCTCTGGTGCTGGTGGAGAACAAAACCGGAAAGGAGCAAAGCCGGA
CTGTCTGGCATGTCGCCACTGACGAAGGCTTAAGCCTGTGGCAGCATACAGTGCTGTCCCTCCTCGATGT
GACTGACAGGTTCTGGCTGCAGATAGTCACATGGTGGGGTCCAGGATCCAGGGCAACCGTGGGATTTGAC
AACATTTCCATCAGCCTCGACTGCTACCTTACCATCAGTGGAGAGGAGAAAATGTCCCTGAATTCAGTAC
CCAAATCTAGAAATCTGTTTGAGAAAAACCCAAACAAGGAGTCAAAATCCTGGGCAAACATATCAGGACC
AACTCCCATCTTCGACCCTACAGTTCACTGGCTGTTCACCACGTGTGGGGCCAGTGGACCTCATGGCCCC
ACCCAGGCACAGTGCAACAACGCCTACCAGAATTCCAACTTGAGCGTGGTGGTGGGAAGTGAAGGGCCCT
TGAAGGGAGTCCAGATTTGGAAAGTGCCAGCTACTGACACCTACAGTATCTCGGGCTACGGAGCAGCTGG
CGGGAAAGGTGGGAAAAACACCATGATGCGGTCCCATGGCGTGTCTGTCCTGGGCATCTTCAATCTGGAG
AAAGGTGACACACTCTACATCCTTGTCGGTCAGCAAGGGGAGGATGCCTGTCCCAGGGCAAACCAACTAA
TCCAGAAAGTCTGTGTGGGTGAGAACAATGTCATAGAAGAAGAGATCCGAGTGAACAGAAGCGTGCACGA
GTGGGCAGGAGGAGGAGGAGGTGGGGGTGGAGCCACCTACGTGTTTAAGATGAAAGATGGCGTGCCTGTA
CCCCTGATCATTGCAGCTGGTGGTGGTGGCAGGGCCTATGGGGCCAAGACAGAAACGTTCCACCCAGAGA
GACTGGAGAGTAACTCCTCGGTTCTAGGGCTGAACGGCAATTCCGGAGCCGCAGGTGGTGGAGGCGGCTG
GAATGATAACACTTCCTTGCTCTGGGCCGGAAAGTCTTTGCTGGAGGGTGCCGCCGGAGGACATTCCTGC
CCCCAGGCCATGAAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCT
CAGGTGGAGGAGGCGGAGGATATATAGGTGGCAACGCAGCATCAAACAATGACCCCGAAATGGATGGGGA
AGATGGGGTTTCCTTCATCAGTCCATTGGGTATCCTGTACACCCCGGCCTTAAAAGTGATGGAGGGCCAC
GGGGAAGTGAATATCAAGCATTATCTAAACTGCAGTCACTGCGAGGTAGACGAATGTCACATGGACCCCG
AGAGCCACAAAGTCATCTGCTTCTGTGATCATGGGACCGTGCTGGCTGATGATGGTGTCTCCTGCATTGT
GTCACCCACCCCGGAGCCCCACCTGCCGCTCTCATTGATCCTCTCCGTCGTGACCTCTGCCCTGGTGGCT
GCCCTTGTTCTGGCATTCTCCGGCATCATGATTGTGTACCGTCGGAAGCACCAGGAGTTGCAGGCTATGC
AGATGGAACTGCAGAGCCCCGAGTATAAGCTGAGCAAGCTACGGACCTCGACCATCATGACCGACTACAA
CCCCAACTACTGCTTCGCTGGCAAGACTTCCTCCATCAGTGACCTGAAAGAAGTGCCACGGAAAAACATC
ACACTCATCCGGGGCCTAGGCCATGGCGCATTTGGGGAGGTGTATGAAGGCCAGGTGTCTGGAATGCCCA
ATGACCCAAGCCCTCTACAAGTGGCTGTAAAGACGCTGCCAGAAGTGTGTTCAGAACAAGATGAGCTGGA
CTTTCTCATGGAAGCTCTGATCATCAGCAAATTCAACCACCAGAATATTGTTCGCTGCATCGGGGTGAGT
CTACAAGCCCTGCCCCGCTTCATCCTGCTGGAACTCATGGCTGGCGGAGACCTCAAGTCCTTCCTCAGGG
AGACACGCCCTCGCCCGAACCAACCCACCTCCCTGGCCATGCTGGACCTTCTGCATGTGGCTCGGGACAT
TGCCTGTGGCTGTCAGTACCTAGAGGAGAATCACTTTATCCACCGGGATATTGCTGCTAGAAACTGTCTG
TTGACCTGCCCAGGAGCTGGAAGAATAGCAAAGATTGGAGACTTTGGGATGGCCCGAGATATCTACAGGG
CCAGCTACTACCGAAAGGGAGGCTGCGCCATGCTGCCGGTCAAGTGGATGCCCCCTGAAGCCTTCATGGA
AGGGATATTTACCTCTAAAACAGACACATGGTCTTTTGGAGTGTTGCTATGGGAAATATTTTCTCTTGGA
TATATGCCGTACCCCAGCAAGAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGCGGAGGACGGATGGACC
CGCCTAAGAACTGCCCCGGGCCTGTATACCGGATAATGACGCAGTGCTGGCAGCATCAGCCTGAAGACAG
ACCCAACTTCGCCATCATTTTGGAGAGGATCGAATACTGCACCCAGGACCCCGATGTGATCAACACAGCT
CTGCCCATCGAATACGGTCCAGTAGTAGAAGAGGAGGAGAAAGTGCCCATGCGCCCCAAAGACCCCGAGG
GGATGCCACCTTTGCTGGTGTCTCCCCAGCCTGCGAAGCACGAGGAGGCGTCCGCAGCTCCCCAGCCCGC
AGCCCTGACGGCACCAGGCCCATCGGTGAAGAAGCCCCCGGGTGCGGGTGCGGGCGCGGGCGCGGGTGCG
GGTGCCGGCCCGGTGCCCCGAGGTGCGGCCGATCGGGGCCACGTGAACATGGCTTTCTCTCAGCCCAACC
CTCCCCCGGAGCTGCACAAAGGCCCGGGATCCAGAAACAAGCCGACCAGCCTGTGGAACCCCACCTACGG
CTCGTGGTTCACCGAGAAGCCTGCCAAAAAGACCCATCCTCCGCCAGGCGCCGAGCCGCAGGCGCGGGCA
GGAGCGGCCGAGGGTGGCTGGACCGGGCCGGGCGCGGGGCCCCGCAGAGCCGAGGCAGCGCTGCTGCTAG
AGCCATCGGCGCTGAGCGCCACCATGAAGGAGGTGCCGCTGTTCAGGCTGCGCCACTTCCCCTGCGGCAA
TGTCAACTATGGTTACCAGCAACAGGGTCTCCCCTTGGAAGCCACAGCCGCGCCAGGGGACACCATGCTG
AAAAGCAAGAATAAGGTCACCCAGCCGGGGCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_007439
Insert Size 4866 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007439.2, NP_031465.2
RefSeq Size 5918 bp
RefSeq ORF 4866 bp
Locus ID 11682
UniProt ID P97793
Gene Summary Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...