Eif4g1 (NM_145941) Mouse Untagged Clone

CAT#: MC224732

Eif4g1 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, (10ug)



  "NM_145941" in other vectors (4)

CNY 20,520.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E030015G23Rik; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; eIF4GI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224732 representing NM_145941
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC
CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC
CCGCCAGGGAGGATTCAGGTCTCTGCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCC
TCCCGAGTGCAGAGTGCAGCCCCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAG
TAATGATGATCCCTTCCCAGATCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCG
TTCCACATATGTTGTCCCAACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCA
AGCCCTACCGAGTTTGGGACCTATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTA
GTGTGGCTCCTGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTAT
CCGAATTCGAGACCCAAACCAAGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCC
TCCACACCCACTCCTCCCCAGACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTG
CTGTCATTATCCGGCCAGATGACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCC
AGAGCATAGCCCTGGCACAGAATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTG
GAGCCGGGGTCTGAGTCTAATCTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATAC
CAATGTCTGTAGAAGAATCGACCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACC
CACTCTTGCCGAACCCATACTGGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCT
TCCAGTCCTCTCCAGGTTTCCACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGA
TCCCATCTGAGGATCTGGAACCAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTG
TGCTTCTGAATCGCTCGTGCCCATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCA
CCACCAGCTGTGGATTTAAGCCCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCAC
TGGCCAGCATTCTCTCTCCTGCTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAAT
GGAAGAAGATGACGATGACGAAGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTT
GACAGTACTCCTGTCCCAGCCCAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGT
CTGTGCCAAAGAGGAGACGGAAAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGC
CTTCAAGGAGGTGGACCCAGCAGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCA
GAGTCTGAGGGCAGCATGGTGCCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACA
AAATTCATAATGCTGAGAACATCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCC
TCTAAACCTTGAAGAAAAGAAGCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGC
ATGCAGAAGCCAGAAGGATTGCCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTC
GGCAACTGGATCCCTCCAGACTACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCT
TGGCCGACCAGCCCTCAGCAACCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCG
GCTGGCCTGGGACCCAGGCGCTCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGA
TAATGACTGAAGACATAAAACTAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGC
TGATAAGGATCGAGGGGAAGAGGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCC
ATCCTGAATAAGCTGACACCCCAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACA
CGGAGGAACGCCTCAAAGGAGTTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGT
GGCTTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACT
GTGAATTTTCGAAAACTGTTGTTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAG
TTTTTGAAAAAAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGA
GCTAGAAGAAGCCCGGGACATAGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTC
AAGTTGAAGATGTTAACAGAAGCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGG
AGTCCCTGGAATGCCTCTGCCGCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCC
TCGAATGGATCAGTATTTCAACCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGT
TTTATGCTGCAGGATGTACTCGATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTA
AGACTATTGATCAAATTCACAAGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCT
CATGGCCAAGGGCAGCGACAAACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTT
GTAGATGATGGTGGCTGGAATACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCA
CCAAGATCACAAAGCCTGGTTCCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTG
GGGCAAGGGCAGCAGTGGGGGCTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCT
ACCTTGAATCGCTTTTCTGCTCTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTAC
AGAGGAGTAGCTTGAGCCGGGAACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGA
GCGGGGAGGTGACCGTGGGGACCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAA
GAAGTGGAGGAGCGAAGTAGAGAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAG
AGGATCGTGGTCGGGATCCTGTGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCT
GTCTGTGGATGAGGTGGAGAAGAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAG
GAGGCAGTACAGTGTGTCCAGGAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCG
AGTCCACGCTGGAGCGTAGTACCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGC
GGGGCACCTCTCTACTGCCCAGTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAA
ATTGATATCCCTCATGTATGGCTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGC
CCATGGGAGAGCTCTTCAGGGAAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCT
GGAGATCCTGGGTCTCTTATGCAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGG
CTGAGCTGGAGGGAATTTCTAGCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAAT
ATACCTTGGGAGAAGAATCTGAAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCT
AGAGAAGCTGCTGAAGGACGGCGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAG
CAGCAGATAGCATCCAATACATTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTG
AGACTCCTCTCCGAGTGGATGTTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGA
TGAGCAGAAGGAGCTACAAGCACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAAC
CTGCTTCGGATGTTCTTTGATGCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGG
AGAGCAGCAAGGATCCTGCTGAACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAA
TTGGCTTCGTGAGGCTGAGGACGAGGAGTCTGATCACAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145941
Insert Size 4803 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145941.3, NP_666053.2
RefSeq Size 5469 bp
RefSeq ORF 4803 bp
Locus ID 208643
UniProt ID Q6NZJ6
Gene Summary This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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