Eif4g1 (NM_145941) Mouse Untagged Clone
CAT#: MC224732
Eif4g1 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, (10ug)
CNY 20,520.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | E030015G23Rik; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; eIF4GI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224732 representing NM_145941
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC CCGCCAGGGAGGATTCAGGTCTCTGCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCC TCCCGAGTGCAGAGTGCAGCCCCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAG TAATGATGATCCCTTCCCAGATCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCG TTCCACATATGTTGTCCCAACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCA AGCCCTACCGAGTTTGGGACCTATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTA GTGTGGCTCCTGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTAT CCGAATTCGAGACCCAAACCAAGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCC TCCACACCCACTCCTCCCCAGACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTG CTGTCATTATCCGGCCAGATGACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCC AGAGCATAGCCCTGGCACAGAATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTG GAGCCGGGGTCTGAGTCTAATCTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATAC CAATGTCTGTAGAAGAATCGACCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACC CACTCTTGCCGAACCCATACTGGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCT TCCAGTCCTCTCCAGGTTTCCACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGA TCCCATCTGAGGATCTGGAACCAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTG TGCTTCTGAATCGCTCGTGCCCATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCA CCACCAGCTGTGGATTTAAGCCCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCAC TGGCCAGCATTCTCTCTCCTGCTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAAT GGAAGAAGATGACGATGACGAAGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTT GACAGTACTCCTGTCCCAGCCCAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGT CTGTGCCAAAGAGGAGACGGAAAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGC CTTCAAGGAGGTGGACCCAGCAGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCA GAGTCTGAGGGCAGCATGGTGCCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACA AAATTCATAATGCTGAGAACATCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCC TCTAAACCTTGAAGAAAAGAAGCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGC ATGCAGAAGCCAGAAGGATTGCCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTC GGCAACTGGATCCCTCCAGACTACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCT TGGCCGACCAGCCCTCAGCAACCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCG GCTGGCCTGGGACCCAGGCGCTCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGA TAATGACTGAAGACATAAAACTAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGC TGATAAGGATCGAGGGGAAGAGGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCC ATCCTGAATAAGCTGACACCCCAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACA CGGAGGAACGCCTCAAAGGAGTTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGT GGCTTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACT GTGAATTTTCGAAAACTGTTGTTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAG TTTTTGAAAAAAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGA GCTAGAAGAAGCCCGGGACATAGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTC AAGTTGAAGATGTTAACAGAAGCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGG AGTCCCTGGAATGCCTCTGCCGCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCC TCGAATGGATCAGTATTTCAACCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGT TTTATGCTGCAGGATGTACTCGATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTA AGACTATTGATCAAATTCACAAGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCT CATGGCCAAGGGCAGCGACAAACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTT GTAGATGATGGTGGCTGGAATACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCA CCAAGATCACAAAGCCTGGTTCCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTG GGGCAAGGGCAGCAGTGGGGGCTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCT ACCTTGAATCGCTTTTCTGCTCTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTAC AGAGGAGTAGCTTGAGCCGGGAACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGA GCGGGGAGGTGACCGTGGGGACCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAA GAAGTGGAGGAGCGAAGTAGAGAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAG AGGATCGTGGTCGGGATCCTGTGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCT GTCTGTGGATGAGGTGGAGAAGAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAG GAGGCAGTACAGTGTGTCCAGGAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCG AGTCCACGCTGGAGCGTAGTACCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGC GGGGCACCTCTCTACTGCCCAGTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAA ATTGATATCCCTCATGTATGGCTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGC CCATGGGAGAGCTCTTCAGGGAAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCT GGAGATCCTGGGTCTCTTATGCAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGG CTGAGCTGGAGGGAATTTCTAGCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAAT ATACCTTGGGAGAAGAATCTGAAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCT AGAGAAGCTGCTGAAGGACGGCGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAG CAGCAGATAGCATCCAATACATTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTG AGACTCCTCTCCGAGTGGATGTTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGA TGAGCAGAAGGAGCTACAAGCACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAAC CTGCTTCGGATGTTCTTTGATGCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGG AGAGCAGCAAGGATCCTGCTGAACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAA TTGGCTTCGTGAGGCTGAGGACGAGGAGTCTGATCACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_145941 |
Insert Size | 4803 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_145941.3, NP_666053.2 |
RefSeq Size | 5469 bp |
RefSeq ORF | 4803 bp |
Locus ID | 208643 |
UniProt ID | Q6NZJ6 |
Gene Summary | This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG212048 | Eif4g1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1 |
CNY 13,300.00 |
|
MR212048 | Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1 |
CNY 10,944.00 |
|
MR212048L3 | Lenti ORF clone of Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1 |
CNY 13,970.00 |
|
MR212048L4 | Lenti ORF clone of Eif4g1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1 |
CNY 13,970.00 |