Eif4g1 (NM_001005331) Mouse Untagged Clone

CAT#: MC224726

Eif4g1 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2, (10ug)



  "NM_001005331" in other vectors (4)

CNY 20,430.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E030015G23Rik; eIF4GI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224726 representing NM_001005331
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC
CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC
CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC
CCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA
TCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCGTTCCACATATGTTGTCCCAAC
ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACCGAGTTTGGGACC
TATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTAGTGTGGCTCCTGCCCCAGTTT
TGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTATCCGAATTCGAGACCCAAACCA
AGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACTCCTCCCCAG
ACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTGCTGTCATTATCCGGCCAGATG
ACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCCAGAGCATAGCCCTGGCACAGA
ATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTGGAGCCGGGGTCTGAGTCTAAT
CTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATACCAATGTCTGTAGAAGAATCGA
CCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACCCACTCTTGCCGAACCCATACT
GGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCTTCCAGTCCTCTCCAGGTTTCC
ACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGATCCCATCTGAGGATCTGGAAC
CAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTGTGCTTCTGAATCGCTCGTGCC
CATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCACCACCAGCTGTGGATTTAAGC
CCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCACTGGCCAGCATTCTCTCTCCTG
CTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAATGGAAGAAGATGACGATGACGA
AGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTTGACAGTACTCCTGTCCCAGCC
CAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGTCTGTGCCAAAGAGGAGACGGA
AAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGCCTTCAAGGAGGTGGACCCAGC
AGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCAGAGTCTGAGGGCAGCATGGTG
CCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACAAAATTCATAATGCTGAGAACA
TCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCCTCTAAACCTTGAAGAAAAGAA
GCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGCATGCAGAAGCCAGAAGGATTG
CCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTCGGCAACTGGATCCCTCCAGAC
TACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCTTGGCCGACCAGCCCTCAGCAA
CCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCGGCTGGCCTGGGACCCAGGCGC
TCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGATAATGACTGAAGACATAAAAC
TAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGCTGATAAGGATCGAGGGGAAGA
GGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCCATCCTGAATAAGCTGACACCC
CAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACACGGAGGAACGCCTCAAAGGAG
TTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGTGGCTTATGCCAACATGTGCCG
CTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACTGTGAATTTTCGAAAACTGTTG
TTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAGTTTTTGAAAAAAAGCAAAAAG
AGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGAGCTAGAAGAAGCCCGGGACAT
AGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTCAAGTTGAAGATGTTAACAGAA
GCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGGAGTCCCTGGAATGCCTCTGCC
GCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCCTCGAATGGATCAGTATTTCAA
CCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGTTTTATGCTGCAGGATGTACTC
GATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTAAGACTATTGATCAAATTCACA
AGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCTCATGGCCAAGGGCAGCGACAA
ACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTTGTAGATGATGGTGGCTGGAAT
ACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCACCAAGATCACAAAGCCTGGTT
CCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTGGGGCAAGGGCAGCAGTGGGGG
CTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCTACCTTGAATCGCTTTTCTGCT
CTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTACAGAGGAGTAGCTTGAGCCGGG
AACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGAGCGGGGAGGTGACCGTGGGGA
CCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAAGAAGTGGAGGAGCGAAGTAGA
GAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAGAGGATCGTGGTCGGGATCCTG
TGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCTGTCTGTGGATGAGGTGGAGAA
GAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAGGAGGCAGTACAGTGTGTCCAG
GAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCGAGTCCACGCTGGAGCGTAGTA
CCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGCGGGGCACCTCTCTACTGCCCA
GTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAAATTGATATCCCTCATGTATGG
CTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGCCCATGGGAGAGCTCTTCAGGG
AAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCTGGAGATCCTGGGTCTCTTATG
CAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGGCTGAGCTGGAGGGAATTTCTA
GCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAATATACCTTGGGAGAAGAATCTG
AAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCTAGAGAAGCTGCTGAAGGACGG
CGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAGCAGCAGATAGCATCCAATACA
TTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTGAGACTCCTCTCCGAGTGGATG
TTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGATGAGCAGAAGGAGCTACAAGC
ACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAACCTGCTTCGGATGTTCTTTGAT
GCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGGAGAGCAGCAAGGATCCTGCTG
AACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAATTGGCTTCGTGAGGCTGAGGA
CGAGGAGTCTGATCACAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001005331
Insert Size 4782 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001005331.1, NP_001005331.1
RefSeq Size 5460 bp
RefSeq ORF 4782 bp
Locus ID 208643
Gene Summary This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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