Eif4g1 (NM_001005331) Mouse Untagged Clone
CAT#: MC224726
Eif4g1 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2, (10ug)
CNY 20,430.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | E030015G23Rik; eIF4GI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224726 representing NM_001005331
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC CCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA TCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCGTTCCACATATGTTGTCCCAAC ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACCGAGTTTGGGACC TATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTAGTGTGGCTCCTGCCCCAGTTT TGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTATCCGAATTCGAGACCCAAACCA AGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACTCCTCCCCAG ACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTGCTGTCATTATCCGGCCAGATG ACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCCAGAGCATAGCCCTGGCACAGA ATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTGGAGCCGGGGTCTGAGTCTAAT CTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATACCAATGTCTGTAGAAGAATCGA CCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACCCACTCTTGCCGAACCCATACT GGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCTTCCAGTCCTCTCCAGGTTTCC ACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGATCCCATCTGAGGATCTGGAAC CAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTGTGCTTCTGAATCGCTCGTGCC CATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCACCACCAGCTGTGGATTTAAGC CCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCACTGGCCAGCATTCTCTCTCCTG CTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAATGGAAGAAGATGACGATGACGA AGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTTGACAGTACTCCTGTCCCAGCC CAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGTCTGTGCCAAAGAGGAGACGGA AAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGCCTTCAAGGAGGTGGACCCAGC AGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCAGAGTCTGAGGGCAGCATGGTG CCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACAAAATTCATAATGCTGAGAACA TCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCCTCTAAACCTTGAAGAAAAGAA GCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGCATGCAGAAGCCAGAAGGATTG CCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTCGGCAACTGGATCCCTCCAGAC TACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCTTGGCCGACCAGCCCTCAGCAA CCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCGGCTGGCCTGGGACCCAGGCGC TCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGATAATGACTGAAGACATAAAAC TAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGCTGATAAGGATCGAGGGGAAGA GGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCCATCCTGAATAAGCTGACACCC CAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACACGGAGGAACGCCTCAAAGGAG TTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGTGGCTTATGCCAACATGTGCCG CTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACTGTGAATTTTCGAAAACTGTTG TTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAGTTTTTGAAAAAAAGCAAAAAG AGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGAGCTAGAAGAAGCCCGGGACAT AGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTCAAGTTGAAGATGTTAACAGAA GCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGGAGTCCCTGGAATGCCTCTGCC GCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCCTCGAATGGATCAGTATTTCAA CCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGTTTTATGCTGCAGGATGTACTC GATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTAAGACTATTGATCAAATTCACA AGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCTCATGGCCAAGGGCAGCGACAA ACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTTGTAGATGATGGTGGCTGGAAT ACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCACCAAGATCACAAAGCCTGGTT CCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTGGGGCAAGGGCAGCAGTGGGGG CTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCTACCTTGAATCGCTTTTCTGCT CTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTACAGAGGAGTAGCTTGAGCCGGG AACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGAGCGGGGAGGTGACCGTGGGGA CCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAAGAAGTGGAGGAGCGAAGTAGA GAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAGAGGATCGTGGTCGGGATCCTG TGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCTGTCTGTGGATGAGGTGGAGAA GAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAGGAGGCAGTACAGTGTGTCCAG GAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCGAGTCCACGCTGGAGCGTAGTA CCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGCGGGGCACCTCTCTACTGCCCA GTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAAATTGATATCCCTCATGTATGG CTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGCCCATGGGAGAGCTCTTCAGGG AAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCTGGAGATCCTGGGTCTCTTATG CAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGGCTGAGCTGGAGGGAATTTCTA GCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAATATACCTTGGGAGAAGAATCTG AAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCTAGAGAAGCTGCTGAAGGACGG CGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAGCAGCAGATAGCATCCAATACA TTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTGAGACTCCTCTCCGAGTGGATG TTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGATGAGCAGAAGGAGCTACAAGC ACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAACCTGCTTCGGATGTTCTTTGAT GCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGGAGAGCAGCAAGGATCCTGCTG AACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAATTGGCTTCGTGAGGCTGAGGA CGAGGAGTCTGATCACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001005331 |
Insert Size | 4782 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001005331.1, NP_001005331.1 |
RefSeq Size | 5460 bp |
RefSeq ORF | 4782 bp |
Locus ID | 208643 |
Gene Summary | This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223361 | Eif4g1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4 gamma 1 (Eif4g1) transcript variant 2, (10ug) |
CNY 17,480.00 |
|
MR223361 | Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
CNY 15,870.00 |
|
MR223361L3 | Lenti ORF clone of Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
CNY 17,770.00 |
|
MR223361L4 | Lenti ORF clone of Eif4g1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2 |
CNY 17,770.00 |