United States
Germany
Japan
United Kingdom
China
Gli3 (NM_008130) Mouse Untagged Clone
CAT＃: MC224709
Gli3 (untagged) - Mouse GLI-Kruppel family member GLI3 (Gli3), (10ug)
CNY 10,840.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | add; AI854843; AU023367; Bph; GLI3-190; GLI3FL; Pdn; Xt |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224709 representing NM_008130
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCAGGCCCACAGCTCTACGGCGACTGAGAGGAAGAAAGCTGAAAATTCCATTGGGAAATGTC CCACGAGAACAGATGTCAGCGAGAAGGCCGTGGCCTCTAGTACCACTTCCAATGAGGATGAAAGTCCTGG ACAGATCTATCACCGAGAGAGAAGAAACGCAATCACTATGCAGCCTCAGAGTGTGCAGGGTCTCAACAAA ATCAGTGAGGAGCCCTCGACGTCTAGTGATGAGAGGGCCTCGCTGATCAAGAAAGAGATCCATGGCTCTC TACCACATCTGGCGGAGCCCTCTCTCCCTTACCGTGGGACTGTGTTTGCCATGGATCCCCGGAATGGCTA CATGGAGCCTCACTACCACCCTCCTCATCTTTTCCCTGCCTTCCATCCTCCTGTACCAATTGATGCCAGA CATCATGAGGGCCGTTACCATTATGATCCATCTCCTATTCCTCCATTACATGTGCCTTCTGCCTTATCTA GTAGCCCGACGTATCCAGACTTGCCCTTCATTAGGATCTCCCCACACCGTAATCCCACTGCAGCCTCAGA GTCCCCCTTCAGCCCCCCACACCCCTACATCAACCCATATATGGACTACATCCGCTCATTGCACAGCAGC CCATCCCTCTCCATGATCTCTGCTGCCCGAGGGCTGAGCCCTACAGATGCTCCCCATGCTGGAGTCAGCC CTGCGGAATACTATCACCAGATGGCTCTGCTGACAGGCCAGCGCAGCCCTTATGCAGACATCCTTCCCTC AGCTGCCACTGCTGGTGCAGGGGCCATCCATATGGAGTACCTTCATGCCATGGACAGCACCAGATTTCCC AGCCCTAGGCTGTCAGCTAGGCCCAGCCGAAAGCGCACACTGTCAATATCGCCACTGTCAGATCATAGCT TCGACCTTCAGACCATGATAAGAACATCCCCTAACTCCTTGGTTACAATCCTCAATAATTCCCGTAGCAG CTCTTCAGCAAGTGGTTCCTATGGGCACTTATCGGCAAGTGCAATCAGCCCTGCATTGAGCTTCACCTAC CCCTCCGCTCCTGTGTCTCTTCACATGCATCAACAGATCCTAAGCCGACAGCAAAGCTTAGGCTCCGCAT TCGGACACAGCCCTCCTCTCATCCACCCTGCTCCAACATTTCCAACACAGAGACCTATCCCTGGGATTCC GACAGTTCTGAACCCCGTCCAGGTCAGCTCTGGCCCTTCTGAGTCCTCACAGAGCAAGCCCACAAGCGAG TCTGCAGTGAGCAGCACTGGTGACCCTATGCATAATAAGCGGTCCAAGATCAAGCCTGATGAAGACCTCC CCAGCCCAGGGTCACGGGGCCAGCAGGAACAGCCGGAAGGAACAACCCTAGTCAAGGAGGAAGCGGACAA AGATGAAAGCAAGCAGGAGCCTGAAGTCATCTACGAGACAAACTGCCACTGGGAAGGCTGCACCAGAGAG TTCGACACCCAAGATCAGCTTGTGCATCATATCAATAATGACCACATTCATGGAGAAAAGAAGGAGTTCG TGTGCCGCTGGCTTGATTGTTCACGAGAGCAGAAACCGTTCAAAGCCCAGTACATGTTGGTAGTGCATAT GAGAAGACACACTGGGGAGAAGCCTCACAAATGTACATTTGAAGGTTGCACAAAAGCCTACTCAAGACTC GAAAACCTGAAAACCCACTTGAGATCTCACACTGGAGAGAAGCCATACGTCTGTGAGCATGAGGGCTGCA ACAAGGCTTTCTCTAATGCTTCAGATCGGGCCAAGCACCAAAACAGAACACATTCCAATGAGAAACCGTA TGTATGCAAAATCCCTGGCTGCACCAAGCGTTACACAGACCCCAGCTCTCTCCGGAAACACGTGAAGACT GTGCATGGCCCTGAGGCTCATGTTACCAAGAAGCAGCGTGGGGACATGCACCCTCGGCCTCCACCCCCGA GAGATTCCGGCAGCCATTCACAGTCCAGGTCCCCTGGCCGGCCAACTCAGGGAGCATTTGGTGAGCAGAA GGAGCTGAGCAACACTACCTCAAAGCGGGAAGAGTGCCTCCAGGTGAAGACTGTCAAGGCTGAGAAGCCC ATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCCATCAGCAACTATT CCAACAGTGGGCTCGAGCTTCCTCTGACTGATGGAGGTAGTGTAGCAGACCTCAGTGCCATCGATGAAAC CCCAATCATGGACTCGACCATTTCCACGGCAACCACAGCCCTTGCTTTGCAAGCCAGGAGAAACCCGGCA GGGACCAAATGGATGGAGCACATCAAACTTGAAAGGCTAAAACAAGTGAATGGAATGTTTCCAAGACTGA ACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATGGCACACAGTCCAATAACAA CTATAGCTCGGGTGGGCCCGGGACCCTCCTCCCGAGCAGAAGTGACCTGTCAGGTGTAGACTTCACAGTG CTGAACACACTCAACAGGAGAGACAGCAATACCAGCACTATCAGCTCTGCCTACCTGAGCAGCCGCAGAT CCTCGGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCAGCGAGGCGTCCCAGGCTGAAGGGCGACCCCA GAATGTGAGTGTGGCTGACTCCTACGATCCCATCTCCACAGATGCTTCAAGGAGGTCCAGCGAGGCCAGC CAGGGTGACGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTACCGCCTTAAGGCCAAGTATG CAGCTGCCACAGGTGGCCCACCACCCACACCTCTGCCCCACATGGAGAGGCTGAGCCTGAAGACCAAGAT GGCCTTGCTAGGGGAAGGGAGGGACTCTGGGGTGACCCTGCCTCCAGTCCATCCTCCTCGAAGATGCAGT GATGGGGGAGGTCACACATACAGAGGGCGTCACCTGATGCCTCATGATGCACTAGCGAACAGTGTGAGGA GAGCCAGCGACCCTGTGAGGACAGTCTCGGAGAACATGTCACTTGCCAGGGTTCAACGCTTCAGCAGCCT CAATAGCTTTAATCCTCCAAATTTGCCTCCATCGGTGGAAAAGCGTAGCTTAGTGCTGCAAAATTATACC CGGCAGGAGAGCAGCCAACCCCGGTACTTCCAGGCATCCCCTTGCCCTCCCAGCATCACAGAGAATGTTG CCCTGGAGGCTCTGACCATGGATGCTGATGCTAACTTGAATGATGAAGACCTCCTGCCAGATGATGTGGT ACAGTATTTAAATTCCCAGAACCAAACAGGGTATGGGCAGCAGTTGCAGAGTGGCATCTCTGAAGACAGC AAAGTAGCCCATGAGCCAGAGGACTTGGACTTAGCAGGGCTGCCAGACAGTCATGTTGGCCAGGAGTACC CAGCTCTGGAGCAGCCCTGCTCTGAGGGCAGTAAAACTGATTTGCCCATCCAGTGGAACGAGGTCAGTTC TGGAACTTCTGATCTGTCATCCTCCAAGCTGAAGTGTGGTCAGCAGCGCCCTAGCGCACAGCAGCCTCGG GGTTTTGGGCTATACAACAATATGGTGGTACACCCACATAATCTGTGGAAGGTTGGCACTGGCCCAGCTG GGGGATATCAGACCCTCGGGGAGAATAGCAGTACCTACAATGGCCCAGAGCACTTTGCAATCCACAGTGG GGATGGACTTGGCACCAATGGAAATACTTTCCATGAACAGCCCTTTAAGACCCAGCAGTATGGGAGCCAG CTCAACAGGCAGCCACTGACTTCCAGTGCTCTAGATCATGCCTGTGGTACAGGGATTCAAGGGTCCAAGC TAAAAGGCAACAGCTTGCAAGAGAATGGGGGTTTGCTAGATTTCAGCCTGTCCGTGGCACCAAATGAGTT AGCTGGCAACACAGTGAATGGCATGCAAACCCAAGATCAAATGGGACAGGGATACATTGCCCATCAGCTA CTCAGTGGCAGCATGCAACACCAGGGGCCCAGCCGCCCTGGTCAACAGGTACTAGGGCAGGTTGGTGCTA CCTCACATATCAACATCTATCAAGGGACAGAGAGCTGCCTACCAGGGACTCAGGACAACAGCAGCCAGCC ATCAAGCATGGCAGCTATCAGGGGCTACCAGCCCTGTGCCAGCTATGGGGGTAACAGGCGTCAGGCAATG CCAAGGGGCAACCTCACTCTGCAACAAGGACAGCTCAGTGACATGAGTCAGAGCAGCAGGGTGAACAGCA TCAAAATGGAGGCACAAGGTCAGTCCCAGCAGCTCTGCTCTACCGTGCAGAATTATTCCGGTCAGTTCTA TGACCAAACCATGGGCTTCAGTCAGCAAGACAGGAAAGCTGGCTCGTTCTCCCTCTCAGATGCCAACTGC CTGCTCCAAGGGAATGGCACTGAAAACTCTGAGTTACTCTCCCCAGGTGCTAACCAGGTAACAAGCACAG TTGACAGCTTTGAGAGTCATGACCTAGAAGGTGTGCAGATTGATTTTGATGCCATCATAGATGATGGGGA CCATACCAGCCTAATGTCAGGGGCCTTGAGCCCAAGTATTATTCAGAACCTTTCCCACAGCTCCTCCCGT CTCACCACTCCGCGGGCATCCCTCCCATTCCCATCCCTATCCATGGGCACCACCAACATGGCTATCGGGG ATATGAGTTCTTTGCTGACCTCCCTTGCAGAAGAAAGCAAGTTCCTTGCAGTTATGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008130 |
| Insert Size | 4752 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008130.2, NP_032156.2 |
| RefSeq Size | 8428 bp |
| RefSeq ORF | 4752 bp |
| Locus ID | 14634 |
| UniProt ID | Q61602 |
| Gene Summary | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226591 | Gli3 (tGFP-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3), (10ug) |
CNY 13,300.00 |
|
| MR226591 | Gli3 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3) |
CNY 10,832.00 |
|
| MR226591L3 | Lenti ORF clone of Gli3 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3) |
CNY 13,970.00 |
|
| MR226591L4 | Lenti ORF clone of Gli3 (mGFP-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3) |
CNY 13,970.00 |
Customer
Reviews
Loading...
