Arhgap35 (NM_172739) Mouse Untagged Clone

CAT#: MC224608

Grlf1 (untagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1), (10ug)



  "NM_172739" in other vectors (4)

CNY 19,190.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6430596G11Rik; AI841135; Grlf1; mKIAA1722; p190A; p190RhoGAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224608 representing NM_172739
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGATGGCAAGAAAGCAAGATGTCCGCATCCCCACCTACAACATCAGTGTGGTGGGATTGTCAGGCA
CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTGTGTAACCGCTTTGTGCGCCCAAGTGC
TGATGAGTTTCACCTGGACCACACTTCTGTCCTTAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT
GACCACTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG
TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT
CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTGGGA
CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA
TTGATGTTAGCAGGGGCATGAACAGGAACTTCGATGATCAGCTCAAATTTGTCTCCAATCTCTACAATCA
GCTTGCAAAAACAAAAAAGCCCATAGTGGTAGTCCTGACTAAGTGTGATGAGGGCGTTGAGCGGTACATT
AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG
TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACAAAAATCAT
CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTA
GTGAGCCGCATTGTGAAAAATCACAATGAGAACTGGCCGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG
AGTACCAGGACTATGTCTACCTGGAAGGGACACAGAAGGCCAAGAAGCTCTTCCTGCAGCACATTCACCG
CCTCAAGCATGAGCATATTGAGCGTCGGAGAAAGCTGTACCTGGCAGCTCTGCCATTGGCTTTTGAAGCC
CTCATACCTAATCTAGATGAAGTGGACCACCTGAGCTGTATTAAAGCAAAAAAGCTGTTAGAGACCAAGC
CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAAACACCATGGGATGCCACCAGCCACATTGACAACAT
GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG
GAGAAGCTGAGGAACGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC
CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG
GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGAGCAAAGGAA
GAGTTCCAAGAGTTGCTTTTGGAGTACTCAGAATTGTTTTATGAGCTGGAGCTGGACGCTAAGCCCAGCA
AGGAGAAGATGGGTGTCATCCAGGATGTTCTGGGCGAAGAGCAGAGATTTAAAGCATTGCAGAAGCTCCA
AGCAGAGCGTGATGCCCTCATTCTGAAGCACATCCATTTTGTGTACCACCCGACGAAGGAGACATGCCCA
AGCTGTCCAGCTTGTGTAGATGCTAAGATTGAACACTTGATCAGTTCTCGCTTTATCAGACCATCTGATC
GGAATCAGAAGAACTCTCTGTCTGACCTCAATATTGATAGGATCAATTTGGTTATCTTAGGCAAAGATGG
CCTTGCCCGAGAGTTGGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT
AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTCCGGCTTCCTGTGAACTCTTTCCAGACAC
CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCACTGTCCTATGTGGTGGAGAG
TATAGAGAAGAGCAGAGAGTCTACACTGGGCAGGCGGGATAATCATTTAGTCCACCTTCCCTTGACCTTA
ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTGATACAGCAAGGTCAGCAGA
TTGCCAGCAAACTTCAGTGTGTCTTTCTTGATCCGGCTTCTGCTGGCATTGGTTATGGACGCAACATTAA
TGAGAAGCAGATCAGTCAAGTTCTGAAAGGACTCCTGGACTCCAAGCGCAACTTAAACCTGGTTAGCTCC
ACTGCAAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTGATGTGTGGTGATCCCT
TTAGTGCAGATGACGTTCTCTCTCCTGTCCTGCAATCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG
CAACTCTGTTTTACTTGAACTTCCAATTGGACTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC
CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA
AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC
ACTCACTGATGGCGCTATAGATGTCCTAGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA
ATTGCACAAGAGATTGACGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTTGAGCTCT
TTCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATTGAGGCCACACATATGTATGATAATGT
AGCTGAGGCCTGCAGCACCACTGAGGAAGTGTTCAACTCCCCCAGAGCTGGCTCACCCCTCTGCAACTCA
AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCATACCACCTGTTTCGGGAAGATGCGACATTGC
CCTCCCTGTCCAAAGATCATTCCAAGTTCTCTATGGAACTGGAGGGAAATGACGGGCTATCTTTCATAAT
GAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCCCCAGTCAAACCAAAGCCTCCTGTGCATTTT
GATATCACAAAAGATCTTTCTTACTTAGACCAAGGCCATCGGGAGGGACAGAGGAAATCTATGTCTTCTA
GCCCCTGGATGCCTCAGGATGGGTTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC
AAGGAATGAGGAAGAAAACATATACTCGGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG
AACATCAACAAAGCCCAGTCCAATGGCAGTGGTAATGGTTCTGACAGTGAGATGGACACAAGTTCCCTAG
AGCGAGGCCGCAAGGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACAAGATGCACTCGCCTGGGGCG
GTTTGCTAGTTACCGTACCAGCTTCAGTGTCGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG
GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCATATGAAACAGATGAGGACCCCA
GGAGGAGGAATATCCTTCGAAGTCTAAGAAGGAACACCAAGAAACCAAAGCCCAAACCCCGGCCATCCAT
CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACAGTGGTGACTCCAGAGAAACCG
ATACCCATTTTCATTGAAAGATGCATTGAGTACATCGAAGCCACAGGACTAAGCACTGAAGGCATCTACC
GGGTCAGTGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGACCACAATCTGGACCT
GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCAGAGCTACCAGAC
CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAAATCAACGACAGGGAACAGAAGC
TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCGAAGGAAAACCATGAAGTCTTCAAATATGTCATCTC
CCACCTGAACAAAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC
TGGCCCACGCTGATGCGGCCTGACTTCAGCAGCATGGACGCGCTCACAGCCACGCGCTCCTACCAGACCA
TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCCATCAGCGAGCCTCCAGGGGC
TGCGCCTGGCTCCCCTTCAGCCATGGCTCCCACTGTTCCCTTCCTCACCTCTACACCTGCTACCAGTCAG
CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTGTCCTCTCAGCTCC
AAGCCGAACACACGCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_172739
Insert Size 4500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172739.4, NP_766327.3
RefSeq Size 8393 bp
RefSeq ORF 4500 bp
Locus ID 232906
UniProt ID Q91YM2
Gene Summary Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:16971514). This binding is inhibited by phosphorylation by PRKCA (By similarity). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (PubMed:11044403, PubMed:11283609, PubMed:18502760, PubMed:21945077). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (PubMed:16971514). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (PubMed:11283609). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (PubMed:11044403, PubMed:26859289, PubMed:18502760). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (PubMed:21945077). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...