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Arhgap35 (NM_172739) Mouse Untagged Clone
CAT＃: MC224608
Grlf1 (untagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1), (10ug)
CNY 19,190.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6430596G11Rik; AI841135; Grlf1; mKIAA1722; p190A; p190RhoGAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224608 representing NM_172739
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGGCAAGAAAGCAAGATGTCCGCATCCCCACCTACAACATCAGTGTGGTGGGATTGTCAGGCA CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTGTGTAACCGCTTTGTGCGCCCAAGTGC TGATGAGTTTCACCTGGACCACACTTCTGTCCTTAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT GACCACTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTGGGA CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA TTGATGTTAGCAGGGGCATGAACAGGAACTTCGATGATCAGCTCAAATTTGTCTCCAATCTCTACAATCA GCTTGCAAAAACAAAAAAGCCCATAGTGGTAGTCCTGACTAAGTGTGATGAGGGCGTTGAGCGGTACATT AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACAAAAATCAT CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTA GTGAGCCGCATTGTGAAAAATCACAATGAGAACTGGCCGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG AGTACCAGGACTATGTCTACCTGGAAGGGACACAGAAGGCCAAGAAGCTCTTCCTGCAGCACATTCACCG CCTCAAGCATGAGCATATTGAGCGTCGGAGAAAGCTGTACCTGGCAGCTCTGCCATTGGCTTTTGAAGCC CTCATACCTAATCTAGATGAAGTGGACCACCTGAGCTGTATTAAAGCAAAAAAGCTGTTAGAGACCAAGC CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAAACACCATGGGATGCCACCAGCCACATTGACAACAT GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG GAGAAGCTGAGGAACGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGAGCAAAGGAA GAGTTCCAAGAGTTGCTTTTGGAGTACTCAGAATTGTTTTATGAGCTGGAGCTGGACGCTAAGCCCAGCA AGGAGAAGATGGGTGTCATCCAGGATGTTCTGGGCGAAGAGCAGAGATTTAAAGCATTGCAGAAGCTCCA AGCAGAGCGTGATGCCCTCATTCTGAAGCACATCCATTTTGTGTACCACCCGACGAAGGAGACATGCCCA AGCTGTCCAGCTTGTGTAGATGCTAAGATTGAACACTTGATCAGTTCTCGCTTTATCAGACCATCTGATC GGAATCAGAAGAACTCTCTGTCTGACCTCAATATTGATAGGATCAATTTGGTTATCTTAGGCAAAGATGG CCTTGCCCGAGAGTTGGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTCCGGCTTCCTGTGAACTCTTTCCAGACAC CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCACTGTCCTATGTGGTGGAGAG TATAGAGAAGAGCAGAGAGTCTACACTGGGCAGGCGGGATAATCATTTAGTCCACCTTCCCTTGACCTTA ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTGATACAGCAAGGTCAGCAGA TTGCCAGCAAACTTCAGTGTGTCTTTCTTGATCCGGCTTCTGCTGGCATTGGTTATGGACGCAACATTAA TGAGAAGCAGATCAGTCAAGTTCTGAAAGGACTCCTGGACTCCAAGCGCAACTTAAACCTGGTTAGCTCC ACTGCAAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTGATGTGTGGTGATCCCT TTAGTGCAGATGACGTTCTCTCTCCTGTCCTGCAATCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG CAACTCTGTTTTACTTGAACTTCCAATTGGACTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC ACTCACTGATGGCGCTATAGATGTCCTAGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA ATTGCACAAGAGATTGACGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTTGAGCTCT TTCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATTGAGGCCACACATATGTATGATAATGT AGCTGAGGCCTGCAGCACCACTGAGGAAGTGTTCAACTCCCCCAGAGCTGGCTCACCCCTCTGCAACTCA AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCATACCACCTGTTTCGGGAAGATGCGACATTGC CCTCCCTGTCCAAAGATCATTCCAAGTTCTCTATGGAACTGGAGGGAAATGACGGGCTATCTTTCATAAT GAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCCCCAGTCAAACCAAAGCCTCCTGTGCATTTT GATATCACAAAAGATCTTTCTTACTTAGACCAAGGCCATCGGGAGGGACAGAGGAAATCTATGTCTTCTA GCCCCTGGATGCCTCAGGATGGGTTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC AAGGAATGAGGAAGAAAACATATACTCGGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG AACATCAACAAAGCCCAGTCCAATGGCAGTGGTAATGGTTCTGACAGTGAGATGGACACAAGTTCCCTAG AGCGAGGCCGCAAGGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACAAGATGCACTCGCCTGGGGCG GTTTGCTAGTTACCGTACCAGCTTCAGTGTCGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCATATGAAACAGATGAGGACCCCA GGAGGAGGAATATCCTTCGAAGTCTAAGAAGGAACACCAAGAAACCAAAGCCCAAACCCCGGCCATCCAT CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACAGTGGTGACTCCAGAGAAACCG ATACCCATTTTCATTGAAAGATGCATTGAGTACATCGAAGCCACAGGACTAAGCACTGAAGGCATCTACC GGGTCAGTGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGACCACAATCTGGACCT GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCAGAGCTACCAGAC CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAAATCAACGACAGGGAACAGAAGC TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCGAAGGAAAACCATGAAGTCTTCAAATATGTCATCTC CCACCTGAACAAAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC TGGCCCACGCTGATGCGGCCTGACTTCAGCAGCATGGACGCGCTCACAGCCACGCGCTCCTACCAGACCA TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCCATCAGCGAGCCTCCAGGGGC TGCGCCTGGCTCCCCTTCAGCCATGGCTCCCACTGTTCCCTTCCTCACCTCTACACCTGCTACCAGTCAG CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTGTCCTCTCAGCTCC AAGCCGAACACACGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172739 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172739.4, NP_766327.3 |
| RefSeq Size | 8393 bp |
| RefSeq ORF | 4500 bp |
| Locus ID | 232906 |
| UniProt ID | Q91YM2 |
| Gene Summary | Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:16971514). This binding is inhibited by phosphorylation by PRKCA (By similarity). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (PubMed:11044403, PubMed:11283609, PubMed:18502760, PubMed:21945077). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (PubMed:16971514). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (PubMed:11283609). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (PubMed:11044403, PubMed:26859289, PubMed:18502760). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (PubMed:21945077). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225830 | Grlf1 (tGFP-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1), (10ug) |
CNY 11,880.00 |
|
| MR225830 | Grlf1 (Myc-DDK-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1) |
CNY 10,264.00 |
|
| MR225830L3 | Lenti ORF clone of Grlf1 (Myc-DDK-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1) |
CNY 12,730.00 |
|
| MR225830L4 | Lenti ORF clone of Grlf1 (mGFP-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1) |
CNY 12,730.00 |
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