Rapgef2 (NM_001099624) Mouse Untagged Clone

CAT#: MC224556

Rapgef2 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), (10ug)



  "NM_001099624" in other vectors (4)

CNY 19,100.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-Rapgef2 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5830453M24Rik; CNRasGEF; mKIAA0313; nRapGEP; Pdzgef1; RA-GEF-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224556 representing NM_001099624
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTCCTTGTGCGTTGCTATGCCAACTGCCTGCAGCCATGGTCCTCCAAACTCCCTGCAGACTTCA
CAAAACTGCACCTCACTGACAGCCTGCACCCACAGGTGACCCACGTGTCCTCCAGCCATTCAGGATGTAG
CATCACCAGCGACTCTGGGAGCAGCAGTCTTTCTGACATTTACCAGGCCACGGAGAGCGAGGCCGGTGAC
ATGGACCTGAGTGGACTGCCAGAGACGGCGGTTGACTCTGAGGATGACGATGATGAGGAAGACATCGAGA
GAGCATCAGACCCTCTGATGAGCAGGGACATCGTGAGGGACTGCCTGGAGAAGGACCCGATAGACAGGAC
TGACGATGACATCGAACAACTACTGGAATTTATGCACCAGCTGCCGGCCTTTGCCAATATGACGATGTCA
GTAAGACGGGAGCTCTGTGCCGTGATGGTGTTTGCAGTGGTGGAAAGAGCTGGAACCATTGTGTTAAACG
ACGGAGAAGAGCTGGATTCCTGGTCAGTGATTCTCAATGGTTCCGTGGAGGTGACATATCCGGATGGGAA
AGCAGAAATTCTGTGCATGGGAAACAGCTTTGGTGTTTCGCCCACCATGGACAAAGAATACATGAAAGGA
GTCATGAGGACTAAGGTGGATGACTGCCAGTTTGTCTGCATTGCCCAGCAAGATTACTGCCGTATTTTGA
ACCAAGTAGAAAAGAACATGCAAAAAGTTGAGGAGGAAGGAGAGATTGTAATGGTGAAGGAGCACCGAGA
ACTTGATCGGACTGGAACAAGGAAGGGCCACATCGTCATCAAGGGCACCTCAGAGAGGCTGACCATGCAT
TTGGTGGAAGAGCATTCTGTAGTGGATCCAACATTCATAGAAGACTTCCTGTTGACATACCGGACTTTCC
TTTCCAGCCCGATGGAAGTGGGCAAGAAGTTATTGGAGTGGTTCAATGACCCGAGCCTCAGGGATAAGGT
GACGCGGGTAGTATTACTGTGGGTGAATAATCACTTCAATGACTTTGAAGGGGATCCTGCAATGACTCGA
TTTCTGGAAGAATTTGAAAATAATCTGGAAAGAGAGAAAATGGGTGGACATCTAAGGCTGTTGAACATTG
CATGTGCAGCTAAAGCCAAACGAAGACTGATGACGTTAACAAAGCCTTCCCGGGAAGCCCCTCTGCCCTT
CATCTTACTGGGAGGCTCTGAAAAGGGCTTTGGCATCTTCGTGGACAGCGTTGACTCGTGTAGCAAAGCC
ACTGAAGCAGGCTTGAAGCGCGGGGATCAGATATTAGAAGTGAATGGTCAAAATTTTGAAAATATCCAAC
TTTCAAAGGCAATGGAAATTCTCAGAAATAACACACACCTGTCCATCACTGTGAAAACCAACTTATTTGT
ATTTAAAGAACTTCTGACGAGATTGTCTGAAGAGAAAAGAAACGGTGCCCCTCACCTCCCTAAAATCGGG
GACATCAAGAAGGCCAGCCGCTACTCCATACCGGATCTCGCCGTAGACGTAGAACAGGTGATAGGCCTCG
AGAAGGTCAACAAGAAAAGCAAAGCCAACACCGTGGGAGGAAGAAACAAGCTGAAGAAAATACTCGACAA
GACCCGGATCAGCATCTTACCTCAGAAACCATACAATGATATTGGGATTGGTCAGTCTCAAGATGATAGC
ATCGTAGGACTGAGGCAGACCAAGCACATCCCAGCTGCCCTGCCTGTCAGTGGAACGCTGTCCTCCAGTA
ACCCTGACTTGCTGCAGTCACACCACCGGATTCTGGACTTCAGCACTACTCCTGACTTGCCAGATCAGGT
ACTGAGAGTTTTCAAGGCTGACCAACAAAGCAGGTACATCATGATCAGCAAGGACACCACCGCAAAGGAA
GTGGTCATCCAGGCCATCAGAGAGTTTGCTGTCACTGCCACCCCAGAGCAGTATTCCCTCTGTGAGGTCT
CAGTCACACCAGAGGGAGTTATCAAACAACGCCGACTCCCAGATCAGCTTTCCAAGCTTGCCGACAGGAT
ACAACTGAGTGGAAGGTATTATCTGAAAAACAACATGGAAACAGAAACGCTTTGCTCAGATGAAGATGCC
CAGGAGCTGCTGCGAGAGAGCCAAATCTCCCTCCTTCAGCTCAGCACCGTGGAGGTTGCCACGCAGCTCT
CCATGAGGAACTTTGAACTCTTCCGCAACATTGAGCCTACTGAATACATAGATGATTTATTTAAACTCAA
ATCAAAAACCAGCTGTGCCAACCTGAAGAAATTTGAGGAAGTCATTAACCAGGAAACATTTTGGGTGGCT
TCTGAAATCCTGCGGGAAACAAACCAGCTGAAGAGGATGAAGATCATTAAGCATTTCATCAAGATTGCCT
TGCACTGCAGGGAATGCAAGAATTTTAACTCCATGTTTGCAATCATCAGTGGCCTAAACCTGGCACCGGT
GGCAAGACTACGAACCACCTGGGAAAAGCTTCCCAATAAATATGAAAAGCTGTTCCAGGACCTGCAAGAC
CTGTTTGATCCTTCCAGAAACATGGCAAAGTACCGCAATGTCCTGAGTGGCCAGAACTTACAGCCTCCTG
TCATTCCCCTGTTCCCTGTCATCAAGAAGGACCTCACGTTTCTCCATGAGGGAAATGACTCAAAAGTAGA
CGGACTGGTCAACTTTGAGAAGCTGAGGATGATTGCAAAAGAAATCCGTCACGTTGGCCGAATGGCATCA
GTCAACATGGACCCTGCCCTTATGTTCAGGACTCGGAAGAAGAAGTGGCGGAGTCTGGGGTCCCTGAGTC
AGGGCAGTGCAAACGCCACAGTGCTGGATGTTGCTCAGACAGGCGGGCATAAAAAGCGGGTACGCCGTAG
TTCCTTCCTCAATGCCAAAAAGCTGTATGAAGATGCCCAGATGGCTCGGAAAGTGAAGCAATACCTGTCC
AACCTGGAGCTGGAGATGGATGAGGAGAGTCTTCAGACACTGTCACTGCAGTGTGAACCAGCAACCAGCA
CATTGCCCAAGAATCCTGGAGACAAAAAGCCTGTCAAGTCTGAGACCTCCCCAGTGGCTCCACGTGCAGG
GCCCCAGCAGAAAGTACAGCCTCAGCAGCCGCTGGCACAGCCACAACCTCCACATAAAGTCAGCCAGGGA
CTGCAGGTGCCCGCTGTGTCCCTCTATCCTTCACGAAAGAAGGTGCCGGTGAAGGATCTGCCTCCTTTTG
GCATAAACTCTCCACAAGCTTTGAAGAAAATCCTGTCCTTGTCTGAAGAAGGGAGTTTGGAGCGTCACAG
GAAGCAAGCAGAAGATACAATATCAAATGCGTCCTCTCAGCTCTCATCGCCCCCCACCTCACCTCAGAGC
TCTCCAAGGAAAGGTTATGCACTGGCTCTCAGTGGCACCGTGGATAATTTCTCAGATTCAGGTCACAGTG
AGATTTCTTCACGATCCAGTATTGTCAGCAATTCTTCTTTTGACTCGGTGCCAGTCTCTCTGCATGATGA
ACGGCGCCAGAGGCATTCTGTCAGCATTGTGGAGAGCAACCTAGGTGTGGGCAGGATGGAGCGGCGGACC
CTGATGGAGCCTGATCAATACAGCTTAGGGTCGTATGCCCCAGTGTCTGAAAGCCGAGGCCTGTATGCCG
CAGCGACGGTCATCTCTTCTCCTAGCACAGAGGAGCTTTCCCATGACCAAGGGGACCGTGCTTCCCTCGA
TGCTGCTGACAGCGGTCGTGGGAGCTGGACATCATGCTCAAGTGGTTCCCATGACAACATACAGACCATT
CAGCACCAGAGAAGCTGGGAAACACTGCCATTTGGGCACACTCACTTTGACTATTCAGGGGATGCTGCAA
GTATATGGGCCTCCGGTGGCCATATGGACCAAATGATGTTTTCTGATCATAGCACAAAATATAACAGACA
GAATCAAAGTAGAGAGAGTCTTGAACAAGCCCAATCCCGGGCCAGCTGGGCGTCTTCCACGGGCTACTGG
GGAGAAGACTCAGAAGGCGACACAGGCACTATCAAGCGAAGGGGTGGGAAGGATGTCTCCGCTGAGGCAG
AGAGCAGCAGCATGGTGCCCGTGACTACAGAGGAAGCCAAACCTGTCCCTATGCCTGCCCACATAGCTGT
GACGCCGAGCACTACCAAGGGACTCATCGCACGGAAGGAAGGCAGGTACCGGGAGCCGCCTCCCACACCT
CCAGGCTACGTGGGCATCCCCATTGCCGATTTCCCAGAAGGGCCTTGCCACCCGGCCAGGAAGCCCCCGG
ATTACAACGTGGCCCTGCAGCGGTCCCGCATGGTGGCACGGCCCACTGAGGCCCCGGCACCGGGCCAGAC
GCCGCCTGCAGCCGCAGCCAGCCGGCCGGGCAGCAAGCCACAGTGGCACAAGCCCAGCGACGCAGACCCA
CGCCTCGCGCCCTTCCAGCCGCAGGGCTTCGCAGGAGCGGAGGAGGACGAAGATGAACAAGTGTCTGCTG
TTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001099624
Insert Size 4485 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001099624.3, NP_001093094.2
RefSeq Size 6583 bp
RefSeq ORF 4485 bp
Locus ID 76089
Gene Summary Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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