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Grin2a (NM_008170) Mouse Untagged Clone
CAT＃: MC224513
Grin2a (untagged) - Mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), (10ug)
CNY 15,032.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | GluN2A; NMDAR2A; NR2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224513 representing NM_008170
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGACTGGGCTACTGGACCTTGCTGGTATTGCCGGCCCTTCTGGTCTGGCACGGTCCGGCGCAGA ACGCGGCGGCGGAGAAGGGTACTCCAGCGCTGAACATTGCGGTGCTGCTGGGTCACAGCCACGACGTGAC AGAACGCGAACTTCGAAATCTGTGGGGCCCGGAGCAGGCAACCGGCTTGCCCCTGGATGTGAACGTGGTG GCGTTATTGATGAACCGCACTGACCCTAAGAGCCTCATCACGCATGTGTGCGACCTCATGTCCGGGGCGC GCATCCATGGCTTGGTGTTTGGAGATGATACGGACCAAGAGGCTGTGGCTCAGATGCTGGATTTTATCTC CTCACAGACTTTCATCCCCATCTTGGGCATTCATGGGGGTGCATCTATGATCATGGCTGACAAGGATCCG ACATCCACGTTCTTCCAGTTTGGAGCTTCCATCCAGCAGCAAGCCACGGTCATGCTGAAGATCATGCAGG ACTATGACTGGCATGTCTTCTCCTTGGTCACCACCATCTTCCCTGGCTACAGAGACTTCATCAGCTTCAT CAAGACAACAGTGGACAACAGCTTTGTGGGCTGGGATATGCAGAACGTGATCACACTGGACACCTCTTTT GAGGACGCCAAGACACAGGTCCAGCTGAAGAAGATCCACTCCTCTGTCATCCTGCTCTACTGCTCCAAGG ATGAGGCTGTCCTCATCCTGAGCGAGGCTCGCTCTCTTGGCCTCACCGGCTACGATTTCTTCTGGATTGT CCCCAGTTTGGTCTCCGGGAACACAGAGCTCATCCCCAAAGAGTTTCCATCGGGTCTCATTTCAGTCTCT TACGACGACTGGGACTACAGTCTGGAGGCAAGAGTGAGAGACGGTCTTGGGATCTTAACCACTGCCGCAT CTTCCATGTTGGAGAAATTCTCCTACATTCCCGAGGCCAAGGCCAGCTGCTACGGGCAGACAGAGAAGCC GGAGACCCCGCTACACACTCTGCACCAATTTATGGTCAATGTGACTTGGGATGGCAAGGACTTGTCCTTC ACTGAGGAAGGCTATCAGGTGCACCCCAGGCTTGTGGTGATCGTGCTGAATAAGGACCGGGAATGGGAAA AGGTGGGCAAGTGGGAGAATCAGACTCTGAGCCTGCGGCATGCTGTGTGGCCAAGGTATAAGTCCTTTTC TGACTGTGAGCCAGATGACAACCACCTCAGCATTGTCACCTTGGAGGAAGCCCCCTTCGTCATCGTAGAA GACATAGACCCACTGACTGAGACCTGCGTCAGGAACACGGTACCCTGTCGGAAGTTTGTCAAGATCAACA ATTCAACCAACGAAGGGATGAATGTGAAGAAGTGCTGCAAGGGGTTCTGCATCGACATCCTTAAGAAACT GTCCAGAACTGTAAAGTTCACCTATGACCTCTACCTGGTGACCAATGGGAAGCATGGGAAAAAGGTTAAC AATGTGTGGAATGGAATGATAGGCGAAGTGGTCTATCAACGAGCAGTTATGGCCGTGGGCTCCCTCACCA TCAATGAGGAGCGTTCAGAAGTGGTGGACTTCTCCGTGCCCTTTGTGGAGACAGGAATCAGTGTCATGGT CTCCAGGAGTAATGGCACTGTTTCCCCTTCTGCTTTCCTTGAACCCTTCAGCGCCTCTGTCTGGGTGATG ATGTTCGTGATGCTGCTCATTGTCTCTGCCATTGCTGTCTTCGTTTTTGAATACTTCAGTCCTGTTGGAT ACAACAGAAACTTAGCCAAAGGGAAAGCTCCCCATGGGCCTTCTTTTACCATTGGAAAAGCTATATGGCT CCTCTGGGGCCTGGTCTTCAACAATTCTGTGCCCGTCCAGAATCCTAAAGGCACAACCAGCAAGATAATG GTATCAGTGTGGGCCTTCTTTGCCGTCATCTTCCTTGCAAGTTACACAGCCAACCTGGCTGCCTTCATGA TCCAGGAGGAGTTTGTGGACCAAGTGACTGGCCTCAGTGACAAGAAGTTCCAGAGACCTCATGACTATTC TCCGCCTTTCCGATTTGGGACAGTACCCAATGGAAGTACAGAAAGGAATATTCGTAACAACTACCCCTAT ATGCACCAGTACATGACCAAATTCAACCAGAGGGGCGTAGAGGATGCCTTGGTCAGCTTGAAAACTGGGA AGTTGGACGCTTTCATCTATGACGCAGCTGTCTTGAACTACAAGGCCGGGAGGGATGAAGGCTGTAAACT GGTGACCATTGGGAGCGGGTACATCTTTGCCACCACAGGCTATGGAATTGCTCTGCAGAAGGGCTCACCC TGGAAGAGGCAGATTGACCTCGCTCTGCTCCAGTTTGTTGGTGACGGTGAGATGGAAGAGCTGGAGACAC TGTGGCTTACGGGCATCTGCCACAACGAGAAGAATGAGGTGATGAGCAGCCAGCTGGACATCGACAACAT GGCAGGAGTTTTCTACATGCTGGCTGCAGCTATGGCCCTCAGCCTCATCACCTTCATCTGGGAGCACCTC TTCTACTGGAAGCTGCGCTTCTGCTTCACAGGCGTGTGTTCTGACCGGCCCGGGCTGCTCTTCTCCATCA GCAGGGGCATCTACAGTTGCATCCATGGAGTGCACATTGAAGAAAAGAAGAAGTCTCCAGACTTCAATCT GACTGGGTCACAGAGCAACATGCTAAAGCTTCTCCGCTCAGCTAAAAACATCTCCAACATGTCCAACATG AACTCCTCGCGAATGGACTCACCCAAAAGAGCTGCTGACTTCATCCAAAGAGGCTCACTTATTGTGGACA TGGTTTCAGACAAGGGAAATTTGATATACTCAGATAACAGGTCCTTTCAAGGGAAGGACAGTATATTTGG AGAAAACATGAATGAACTGCAAACATTTGTGGCCAACAGGCACAAGGATAGTCTCAGTAACTATGTGTTT CAGGGACAGCATCCTCTCACTCTCAATGAGTCCAACCCCAACACAGTGGAGGTGGCTGTCAGCACTGAAT CCAAAGGGAACTCCCGACCCCGGCAGCTTTGGAAGAAATCCATGGAGTCTCTACGCCAGGATTCTCTAAA CCAGAACCCAGTCTCCCAGAGGGATGAGAAGACTGCAGAGAATAGGACCCACTCCCTAAAGAGTCCTAGG TATCTTCCAGAAGAGGTAGCCCATTCTGACATTTCTGAAACCTCAAGCCGGGCCACATGCCACAGGGAGC CAGATAATAATAAGAACCACAAGACCAAGGATAACTTCAAAAGGTCAATGGCCTCTAAATACCCCAAGGA CTGTAGTGAGGTTGAACGTACCTACGTGAAAACCAAAGCAAGTTCTCCCAGGGATAAGATCTACACCATC GATGGTGAGAAGGAGCCCAGCTTCCACTTAGATCCTCCACAGTTCATTGAAAACATAGTCTTGCCTGAGA ATGTGGACTTCCCAGATACCTACCAAGATCACAATGAGAATTTCCGCAAGGGGGACTCCACACTGCCCAT GAACAGGAACCCACTACACAATGAAGATGGGCTTCCCAACAATGACCAGTATAAACTCTATGCCAAGCAC TTTACCTTGAAAGACAAGGGTTCCCCACATAGTGAGGGCAGTGATCGATATCGGCAGAACTCCACGCATT GCAGAAGCTGCCTCTCAAACCTGCCCACCTACTCAGGCCACTTTACCATGAGATCTCCTTTCAAGTGTGA TGCCTGTCTGCGGATGGGGAACCTCTATGACATTGATGAAGACCAGATGCTTCAGGAGACAGGCAACCCA GCTACTCGTGAGGAGGCCTACCAGCAGGACTGGTCACAGAACAACGCCCTCCAGTTCCAGAAGAACAAGC TAAAGATTAATCGACAGCACTCCTATGATAACATTCTCGACAAACCCAGGGAGATAGACCTTAGCAGGCC CTCTCGTAGCATAAGCCTCAAGGACAGGGAAAGGCTACTGGAGGGCAACTTATACGGGAGCCTGTTCAGT GTCCCCTCAAGCAAACTCTTGGGGAACAAAAGCTCCCTTTTCCCCCAAGGTCTGGAGGACAGCAAGAGGA GCAAATCTCTCTTGCCAGACCATACCTCTGATAATCCTTTCCTCCACACGTACGGGGATGACCAACGCTT AGTTATTGGGAGATGTCCCTCGGACCCTTACAAACACTCATTGCCATCACAGGCAGTAAATGACAGCTAT CTTCGGTCATCCTTGAGGTCAACAGCATCATATTGCTCCAGGGACAGTCGGGGCCACAGTGATGTGTATA TTTCAGAGCATGTTATGCCTTATGCTGCAAATAAGAATAACATGTACTCTACCCCCAGGGTTTTAAATTC CTGCAGCAATAGACGTGTGTACAAGAAAATGCCTAGTATTGAATCTGATGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008170 |
| Insert Size | 4395 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008170.2, NP_032196.2 |
| RefSeq Size | 4512 bp |
| RefSeq ORF | 4395 bp |
| Locus ID | 14811 |
| UniProt ID | P35436 |
| Gene Summary | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium (PubMed:1374164). Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (By similarity). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (PubMed:7816096, PubMed:8987814).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227135 | Grin2a (tGFP-tagged) - Mouse glutamate receptor ionotropic NMDA2A (epsilon 1) (Grin2a), (10ug) |
CNY 16,060.00 |
|
| MR227135 | Grin2a (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a) |
CNY 15,024.00 |
|
| MR227135L3 | Lenti ORF clone of Grin2a (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a) |
CNY 16,530.00 |
|
| MR227135L4 | Lenti ORF clone of Grin2a (mGFP-tagged) - Mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a) |
CNY 16,530.00 |
Customer
Reviews
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