Cdk13 (NM_027118) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310015O17Rik; Cdc2l5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224487 representing NM_027118 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGTAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCCGAGAAGAAGTTGGAGG AGCGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCCCCCGGCGCGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCTTGCTTCAGCCCGGGCCCTCCTCTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCCCCGGAGGGCGGCAGAAGCGGCGCCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGCCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCCAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAGTACGAGGATGTGAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGCGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAACGGCGGGAGTCCGGCCTCCTCCTCCGGCAC GCAGAGGCGCGCGGAGGGGTCGGAGCGCAGGCCGCGCCGGGACCGCCGCAGCAGCAGCGGCCGCAGCAAG GAGCGCCACCGCGAGCACCGGCGGCGGGACGGGACGCGCAGCGGCAGCGAGGCCTCAAAGGCCCGCAGCC GCCACGGACACAGCGGCGAGGAGCGGGCGGAGGCCGCCAAGAGCGGCAGCAGCAGCAGCAGCGGCGGCCG CCGCAAGAGCGCGTCGGCTACGTCCAGCAGCAGTAGCAGCCGCAAGGACCGGGACCTCAAGGCCCACCGC AGCCGGACTAAGTCGTCCAAGGAACCGCCCTCGGCCTACAAGGAGCCGCCCAAGGCCTACCGGGAGGACA AGAGCGAGCCGAAGGCCTACAGGCGGCGGCAGCGGTCCCTGAGCCCGCTGGGAGGCCGGGACGAAAGCCC GGTGTCCCACAGGGCCTCGCAGAGCCTTCGGAGCCGCAAGTCCCCCAGTCCGGCGGGAGGTGGCAGCAGT CCTTATTCCCGGCGGCTGCCGCGCTCCCCGAGCCCCTATAGCCGGCGCCGCTCGCCCAGCTACAGCCGCC ACAGCTCCTACGAGCGGGGCGGCGACGTATCCCCCAGCCCGTACAGCAGCAGCAGCTGGCGGCGCTCGCG CAGCCCCTACAGCCCAGTACTCAGACGATCTGCCAAATCCCGAAGCAGAAGCCCATATTCATCTAGGCAC TCAAGATCTCGGAGCAGGCACAGATTGTCTAGATCAAGAAGTCGTCATTCAAGCATTTCTCCTAGCACAC TAACTCTGAAGAGTAGCTTGGCAGCTGAATTGAACAAGAATAAAAAGGCACGAGCTGCAGAGGCAGCAAG AGCTGCAGAGGCAGCAAAAGCTGCAGAAGCTGCTAAGGCTGCTGAAGCAGCTGCCAAAGCTGCCAAAGCC TCAAATGCTTCTACACCTACCAAGGGGAACACAGAAACTGGTGCCAGTGTCTCACAGACAAACCATGTGA AGGAAGTCAAAAAACTTAAAACTGAGCATGCACCTTCTCCTTCAAGTGGTGGGACCGTCAAAAGCGACAA AGCAAAAACAAAGCCACCGCTTCAAGTAACAAAGGTAGACAATAATTTGACAGTAGAGAAAGCCACCAAG AAAACAGTTGTTGGGAAGGAGAGTAAACCTGCTGCTACAAAGGAAGAACCAGTTTCCACTAAAGAGAAAA GCAAGCCACTCACACCAAGCACAGGAGCCAAGGAGAAGGAGCAGCATGTGGCTTTAGTGACCTCTACGTT ACCGCCATTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGATGCTGATAGCTTAAGAGGCAACATTTCT GTCAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTGCTTGCTGATTTACCATTGCCCCCTG AGTTACCAGGAGGAGATGATCTTTCCAAGAGTCCAGAGGAGAAGAAAACAGCAGCACAGTTACATAGCAA ACGAAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAATCAAAGAAAAAGATATTGACTGGGGGAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTATGGACAAGTTTACAAAGCCAGGG ACAAAGACACGGGAGAAATGGTAGCCTTAAAGAAAGTACGTCTGGATAATGAAAAGGAGGGTTTCCCAAT TACAGCAATTAGAGAAATTAAAATTCTTCGGCAACTCACCCACCAGAGTATCATCAATATGAAGGAAATC GTGACTGATAAAGAAGATGCTTTGGATTTTAAGAAAGACAAAGGTGCATTTTACCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTGCTGGAATCAGGCTTGGTTCATTTTAATGAAAACCATATAAAATCTTT TATGAGACAGCTCATGGAAGGCCTGGATTATTGTCATAAGAAGAACTTTTTGCATAGAGATATTAAATGT TCAAATATCCTTCTAAATAATAGAGGACAGATAAAACTTGCAGATTTTGGACTTGCTCGTTTATATAGCT CAGAAGAAAGTCGCCCATATACTAACAAGGTCATTACTTTGTGGTATCGTCCACCTGAATTGCTCTTGGG AGAAGAACGATATACACCAGCCATTGATGTATGGAGCTGTGGATGTATCCTTGGTGAACTCTTCACTAAA AAACCTATATTTCAAGCAAACCAGGAACTTGCACAGCTAGAGCTAATAAGCCGTATATGTGGGAGTCCAT GTCCTGCAGTGTGGCCTGATGTAATCAAACTGCCATATTTCAACACCATGAAACCAAAGAAGCAATATCG GCGGAAGTTAAGAGAAGAATTTGTTTTCATCCCCGCAGCTGCACTCGACTTATTCGATTACATGCTTGCC TTGGATCCCAGTAAGCGCTGCACTGCTGAGCAGGCTCTTCAGTGTGAGTTCCTGCGAGACGTGGAACCCT CCAAAATGCCTCCACCAGACCTTCCTTTGTGGCAAGATTGTCATGAATTATGGAGTAAAAAGAGAAGAAG ACAGAAACAGATGGGCATGACTGATGATCTTTCCACAATCAAAGCCCCTAGGAAGGACCTGTCTCTGGGC TTAGATGACAGCAGAACTAACACACCCCAGGGTGTGCTGCCACCCGCACAGTTGAAATCTCAGAGCAACT CAAATGTAGCACCTGGTGAAAAACAGACAGATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGG AGGAGTTCAGCCTTCACAGACCATCCAGCCTAAAGTGGAAACTGATGCTGCCCAGGCTGCTGTGCAGAGT GCATTTGCAGTCCTCTTGACTCAGTTAATAAAGGCCCAACAGTCCAAACAGAAAGATGCCATGCTAGAGG AAAGGGAAAATGGATCAGGACATGAAGCTCCATTGCAACTCAGGCCTCCTCTAGAACCGAGCACTCCTGG ATCCGGGCAAGATGACCTCATCCAGCACCAAGACAGGAGGATATTGGAGCTGACACCAGAACCAGACCGG CCTCGAATTCTGCCTCCTGATCAACGGCCTCCTGAACCTCCTGAACCACCACCAGTTACTGAGGAGGACC TAGATTATCGGACAGAAAACCAGCATGTTCCTACCACTAGTTCTTCATTAACTGACCCACATGCTGGAGT GAAGGCAGCCCTCTTACAGCTGCTTGCTCAGCATCAGCCCCAGGATGATCCCAAAAGAGAAGGTGGTATC GATTATCCCACAGGAGACACATATGTGCCCAGTTCAGACTATAAGGACAACTTTGGATCTTCTTTCTCTG CCGCTCCTTACGTTAGCAGTGATGGTCTAGGAAGTAGCTCCGCTGCTGCACCATTGGAAGCACGTAGTTT CATTGGAAACTCAGATATTCAGTCTCTGGATAACTACAGTACTGCTTCATCTCACACTGGTGGTCCACCT CAAACTTCTGCCTTTACTGAGTCGTTTGCCAGTTCAGTAGCTGGATATGGAGACATTTACCTCAATGCTG GTCCCATGTTGTTTAGTGGAGACAAGGACCATAGATTTGAATATAGCCATGGTCCTATCACAGTCCTCAC AAACAGCAATGACCCTTCCACAGGGCCAGAGAGTACTCATCCCTTGCCAGCAAAGATGCACAACTATAAC TATGGTGGTAACTTACAGGAAAATCCAGGTGGCCCTAGCCTCATGCATGGACAGACCTGGACTTCTCCTG CCCAAGGACCTGGATATTCACAAGGATACAGGGGACACATTAGCACATCAGCTGGCAGAGGTCGAGGCAG AGGGTTACCATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027118
Insert Size	4356 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027118.1, NP_081394.1
RefSeq Size	6901 bp
RefSeq ORF	4356 bp
Locus ID	69562
UniProt ID	Q69ZA1
Gene Summary	Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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