Dcc (NM_007831) Mouse Untagged Clone

CAT#: MC224479

Dcc (untagged) - Mouse deleted in colorectal carcinoma (Dcc), (10ug)



  "NM_007831" in other vectors (4)

CNY 18,620.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C030036D22Rik; Igdcc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224479 representing NM_007831
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATAGTCTTGGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCA
GCGCACATCTTCAAGTCACGGGTTTCCAAATTAAACCTTTCACGTCACTTCATTTTGTGTCTGAACCTTC
GGATGCTGTCACCATGCGGGGAGGAAATGTCCTGCTCAACTGCTCTGCAGAGTCCGACCGAGGAGTTCCA
GTCATCAAGTGGAAGAAAGACGGCCTTATCCTAGCCTTGGGCATGGATGACAGAAAGCAGCAACTTCCTA
ATGGATCTCTGTTGATACAAAACATACTTCATTCCAGACACCATAAGCCGGATGAAGGACTTTACCAATG
CGAGGCATCCTTAGCAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTACGGTAGCAGGGCCATTG
AGGTTCCTTTCCCAGACGGAATCTATCACAGCCTTCATGGGAGATACAGTGTTACTTAAGTGTGAAGTCA
TTGGGGAACCCATGCCAACAATACACTGGCAGAAAAATCAACAAGACCTCAACCCACTCCCAGGAGACTC
GCGTGTGGTCGTCTTGCCCTCCGGAGCATTGCAGATTAGCCGACTTCAGCCAGGAGACAGTGGAGTCTAC
AGATGCTCGGCTCGGAATCCAGCCAGCATAAGAACAGGAAATGAAGCAGAAGTTAGAATTTTATCAGACC
CAGGCCTGCATAGGCAGCTATATTTTCTGCAGAGACCATCCAACGTAATAGCCATTGAAGGGAAAGATGC
TGTCCTGGAGTGCTGTGTTTCTGGCTATCCTCCGCCAAGTTTCACCTGGTTACGAGGCGAGGAGGTCATC
CAACTCAGGTCTAAAAAGTATTCATTGTTGGGTGGAAGCAATTTGCTCATCTCTAATGTGACAGATGATG
ACAGTGGAACCTATACTTGCGTTGTCACCTATAAGAATGAGAATATTAGTGCCTCTGCAGAGCTCACGGT
CTTGGTTCCGCCCTGGTTTTTAAATCACCCTTCCAACCTCTATGCCTATGAGAGCATGGATATTGAGTTT
GAATGTGCAGTTTCTGGGAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTGATCCCGA
GTGACTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATCCTTGGGGTGGTCAAGTCAGATGAAGGCTT
CTATCAATGTGTGGCTGAAAATGAGGCTGGCAATGCCCAAAGTAGTGCCCAGCTGATTGTCCCCAAGCCT
GCCATCCCAAGCTCCAGCATCCTCCCTTCTGCTCCCAGAGATGTGCTCCCTGTCTTGGTTTCTAGTCGCT
TTGTCCGTTTGAGCTGGCGTCCACCTGCAGAAGCAAAAGGGAACATTCAAACCTTCACAGTCTTTTTCTC
CAGAGAGGGTGACAACAGGGAGCGAGCTTTGAATACAACCCAGCCTGGGTCCCTTCAGCTTACTGTGGGA
AACTTGAAACCAGAAGCCATGTACACCTTCAGAGTTGTCGCCTACAATGAGTGGGGACCAGGGGAGAGTT
CTCAGCCCATCAAGGTGGCCACTCAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCATGCTGT
ATCTACCTCACCTACCTCAATTCTTATTACATGGGAACCCCCTGCCTATGCAAATGGCCCAGTCCAAGGT
TACAGATTGTTCTGCACTGAGGTGTCTACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATA
AACTGGAAGGCCTGAAAAAATTCACGGAATATACTCTTCGATTCCTAGCTTATAATCGTTATGGGCCAGG
AGTATCTACTGATGATATAACAGTTGTTACACTTTCAGACGTGCCAAGTGCTCCACCTCAGAACATCTCA
CTGGAAGTGGTTAACTCAAGGAGTATCAAAGTAAGCTGGCTCCCTCCTCCATCGGGAACACAAAATGGAT
TTATTACTGGCTATAAAATTCGACACAGAAAGACTACCCGCAGGGGCGAGATGGAAACACTGGAACCGAA
TAACCTCTGGTACCTCTTCACAGGATTGGAGAAAGGCAGTCAGTACAGTTTCCAAGTGTCGGCCATGACA
GTCAATGGTACTGGACCACCTTCCAACTGGTACACAGCGGAGACCCCAGAGAATGATTTAGATGAGTCTC
AAGTCCCTGACCAGCCGAGTTCTCTTCACGTGAGGCCCCAGACAAACTGTATCATCATGAGTTGGACACC
TCCTCTCAACCCAAACATCGTGGTGAGAGGCTATATTATTGGCTATGGTGTTGGCAGTCCTTATGCTGAA
ACAGTGCGTGTGGACAGTAAGCAGCGGTACTATTCCATCGAGAGACTAGAGTCGAGTTCTCATTATGTAA
TCTCCTTAAAAGCTTTCAACAATGCCGGAGAAGGTGTCCCTCTCTACGAAAGTGCCACCACTCGTTCCAT
AACAGATCCCACTGACCCCGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGGCCCAGATGTC
TCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGAGGCTGTGAGGGTCAGCT
GGGCAGACAACTCTGTCCCGAAGAACCAGAAAACATCCGATGTGCGACTGTACACCGTTCGGTGGAGGAC
CAGCTTTTCTGCCAGTGCCAAGTACAAGTCAGAAGACACAACATCTCTCAGTTACACAGCGACTGGCCTC
AAGCCCAATACCATGTACGAGTTCTCAGTCATGGTGACCAAGAACAGAAGGTCAAGCACGTGGAGCATGA
CTGCACACGCCACCACCTACGAAGCTGCCCCAACTTCTGCTCCCAAGGATCTGACAGTCATTACTCGAGA
GGGGAAGCCTCGTGCTGTCATTGTGAGCTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATCACTGCTTAT
ATCTTGTTTTATACATTGGACAAGAATATACCAATAGATGATTGGATTATGGAAACTATCAGCGGTGACC
GGCTTACCCATCAGATCATGGATCTCAGCCTTGACACGATGTATTACTTTCGAATTCAAGCACGGAATGT
GAAAGGAGTAGGGCCCCTCTCTGATCCCATCCTCTTCAGGACTTTGAAAGTGGAACACCCTGACAAAATG
GCTAATGACCAAGGTCGTCATGGAGATGGGGGTTATTGGCCAGTTGATACTAACTTGATTGATAGAAGCA
CTCTCAACGAACCACCCATTGGACAGATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAA
CCTGCTTGTAATAACCGTGGTCACCGTTGGTGTTCTCACTGTGTTGGTCGTGGTGATTGTAGCAGTGATT
TGCACCCGGCGTTCTTCAGCGCAGCAGAGGAAGAAACGGGCCACACACAGTGTGAGCAAGAGGAAGGGCA
GTCAGAAGGACCTCCGGCCTCCCGATCTTTGGATACATCATGAAGAAATGGAAATGAAAAATATCGAGAA
GCCTACGGGAACCGACCCTGCAGGAAGAGACTCCCCCATCCAGAGCTGCCAAGATCTCACACCAGTCAGC
CATAGCCAGTCAGAAACCCAGATGGGAAGCAAAAGTGCCTCTCATTCAGGTCAGGACACTGAGGACGCAG
GCAGCTCCATGTCCACTTTGGAACGGTCCCTGGCAGCACGCCGGGCCACCAGGGCCAAGCTCATGATTCC
CATGGAGGCCCAGTCCAGTAATCCTGCTGTTGTGAGTGCCATCCCTGTACCAACACTAGAAAGTGCCCAG
TATCCAGGAATCCTCCCATCTCCCACATGTGGATACCCGCATCCACAGTTCACTCTCCGGCCAGTACCAT
TCCCAACGCTGTCTGTGGACCGAGGTTTTGGAGCAGGAAGAACTCAGTCTGTGAGTGAAGGACCAACCAC
GCAACAGCAACCCATGCTGCCCCCAGCTCAGCCCGAACATCCGAGCAGTGAAGAAGCACCCAGTAGAACC
ATCCCCACAGCCTGTGTGCGGCCAACTCATCCACTCCGCAGCTTTGCTAACCCATTACTACCTCCACCCA
TGAGTGCAATAGAACCAAAAGTCCCTTACACACCGCTTTTGTCTCAGCCAGGACCCACCCTTCCCAAGAC
TCATGTTAAAACGGCCTCCCTTGGGCTGGCCGGAAAGGCAAGGTCCCCTTTGCTTCCCGTGTCTGTGCCA
ACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAGCCCACAGAGGATCCAGCCAGTGTATATGAACAGGATG
ACTTGAGCGAACAAATGGCAAGTTTGGAAGGGCTAATGAAGCAACTCAACGCCATCACAGGGTCTGCCTT
TTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007831
Insert Size 4344 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007831.3, NP_031857.2
RefSeq Size 10325 bp
RefSeq ORF 4344 bp
Locus ID 13176
UniProt ID P70211
Gene Summary Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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