Syne1 (NM_153399) Mouse Untagged Clone

CAT#: MC224453

Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 1, (10ug)



  "NM_153399" in other vectors (4)

CNY 18,430.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224453 representing NM_153399
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACCTCCAGAGCATCTTCCCGGTCCCATCGGGACATCACCAATGTGATGCAGAGGCTACAAGATG
AACAAGAGATTGTACAGAAACGTACCTTCACAAAATGGATCAATTCCCATCTGGCCAAGCGGAAGCCTCC
CATGGTGGTGGATGACCTTTTTGAAGACATGAAAGATGGCATCAAGCTGCTTGCTTTACTGGAGGTCCTG
TCTGGGCAGAAACTGCCTTGTGAACAAGGACACCGGGTGAAGCGTATTCATGCTGTGGCTAACATTGGCA
CCGCACTCAAATTCCTTGAAGGAAGGAAGTCCATGTACAGAGGATCACCGATTAAATTAGTCAACATTAA
TGCTACTGATATTGCTGATGGCCGACCATCAATCGTTCTTGGGCTGATGTGGACCATTATCCTGTATTTC
CAGATAGAAGAGTTGACCAGCAACCTACCACAGCTGCAGTCTCTATCCAGCAGTGCTTCCTCTGTGGACA
GCATGGTCAGCACCGAGACTGCCAGCCCACCCAGCAAACGAAAGGTGGCTGCCAAGATCCAGGGAAATGC
CAAGAAAACTCTATTAAAGTGGGTCCAGCACACAGCAGGCAAGCAGATGGGAATAGAAGTGAAAGACTTC
GGGAAGAGTTGGAGAACTGGGTTGGCCTTTCATTCAGTCATCCACGCTATCCAGCCTGAGCTGGTGGACC
TGGAGAAGGTGAAGACTAGATCTAACAGGGAAAACCTGGAGGACGCCTTCACGATCGCTGAAACACAGCT
AGGCATCCCCAGACTGCTTGATCCCGAAGATGTGGATGTGGACAAGCCAGATGAAAAGTCTATCATGACT
TACGTGGCCCAGTTTCTAACACAATACCCTGACATTCATGGTGCAGGCTGTGATGGGCAAGAGGATGATA
GGGATGACAGACTAATTTTGAAGGAAACAAAAGTTTGGATAGAACAATTTGAGAGAGATTTTACAAGGGC
ACAGATGACCGAATCAAGTCTGCAGGACAAATATCAGGCATTTAAGCACTTCAGAGTTCAATATGAAATG
AAAAGGAAACAAGTGGAGCATATAATACAGCCATTGCAAAGAGATGGCAAACTGACGCTCGACCAAGCCT
TGGTCAAGCAGTGCTGGGAGAGAGTATCATCCCGGCTCTTCGATTGGCATATACAGCTGGATAAATCTCT
CCCTGCACCTCTGGGCACTATAGGTGCTTGGCTATACAGGGCAGAAGTGGCCCTGAGAGAAGAGATCACC
ATTCAGCAGGTTCACGAGGAGACAGCAAACACAATCCAACGGAAACTTGAGCAACATAAGGATCTGCTTC
AAAATACAGATGCCCACAAAAGGGCGTTCCATGAAATCTACCAGACCAGGTCTGTCAATGGGATCCCGAT
GCCTCCTGATCAGCTAGAGGACATGGCTGAGAGGTTTCATTTTGTGTCCTCCACATCCGAGCTGCACTTA
ATGAAAATGGAGTTTCTCGAACTGAAGTACCGTCTGCTGTCCCTGCTGGTCCTCGCTGAGTCAAAGCTGA
AGTCCTGGATCATCAAGTATGGGAGGCGAGAGTCTGTGGAGCTGCTGCTGCAAAGCTATATCTCTTTTAT
AGAAAACAGCAAGTTCTTTGAACAGTATGAAGTGACATACCAGATCCTGAAACAGACAGCTGACATATAC
GTCAAAGCAGAGGGCTCAGTGGAGGAAGCTGAGAACGTGATGAAATTCATGAGTGAAGCCACTGCCCAGT
GGAGGAACCTCTCCGTGGAAGTGCGGAGTGTACGGAGCATGCTGGAGGAGGTGATCTCCAACTGGGATCG
CTATGGTGATACCGTGGCTAGTCTCCAAGCCTGGCTAGAAGATGCAGAGAAAATGCTAAGCCAATCAGAA
CATGCCAAGAAGGATTTTTTTCGAAATCTGCCTCACTGGATTCAGCAGCACACAGCCATGAACGATGCCG
GGAATTTCCTGATTGAAACCTGTGATGAGATTGTTTCTCGAGACCTTAAACAGCAGTTGCTCTTATTGAA
CGGTCGATGGAGGGAGCTCTTCATGGAAGTCAAGCAATATGCAAGAGCAGACGAGATGGACAGAATGAAG
AAAGAGTATATAGACGTCACTACCACCCTCTTTGGTTTTGCAACTGAAGCCCACAGGAAACTCTCTGAGC
CTTTAGAAGTCTCTTTTATCAATGTCAAGTTATTAATTCAAGACTTGGAGGATCTGGAGAAGAGGGTGCC
TGTGATGGATGCTCAGTACAAGATGATTGCCAAGAAGGCACATCTCTTTGCCAAAGAAAGTCCCCAAGAA
GAAGCGAATGAAATGCTTACAACCATGTCCAAGCTCAAAGAACAGCTCTCCAAGGTCAAAGAATGTTGCT
CCCCACTGTTGTACGAGGCCCAGCAACTGACGGTTCCTTTGGAAGAACTGGAGACGCAAATCACGTCCTT
CTATGACTCCCTTGGAAAAATCAATGAGATTCTATCAGTTCTTGAGCAAGAAGCACAATCTAGCACTCTT
TTTAAGCAAAAACACCAGGAACTGTTAGCTTCTCAAGAGAACTGTAAGAAATCCTTGACACTCATAGAGA
AGGGCAGCCAAAGTGTTCAGAAGCTGGTGACCTCGAGCCAGGCACGAAAGCCCTGGGATCACACAAAACT
GCAGAAGCAGATAGCAGATGTCCATCATGCATTCCAGAGCATGATCAAGAAAACTGGAGACTGGAAGAAG
CACGTGGAAGCCAACAGCCGTTTGATGAAGAAATTTGAGGAGTCTCGGGCCGAGTTGGAGAAGGTGCTTA
GGGTTGCTCAGGAGGGCCTAGAGGAGAAGGGGGACCCCGAGGAACTCCTTCGGAGACACACGGAGTTCTT
CAGCCAACTGGACCAGAGGGTCCTCAATGCTTTCCTAAAAGCCTGTGATGAGCTAACTGACATCCTCCCA
GAGCAGGAGCAACAAGGCCTACAGGAGGCTGTTCGAAAGCTACACAAACAGTGGAAGGATCTTCAGGGAG
AAGCGCCTTATCATTTGCTCCATCTGAAAATCGCTGTGGAGAAAGACAGATTCTCAGCTGCTGTGGAAGA
GTGCAGAGCTGAGCTGGAGCAAGAGACCAAGCTGGCTCCTCAAGAAGGCAGCGAGAAGATCATAAAGGAA
CACAGGGTTTTCTTCAGTGACAAGGGTCCTCACCATCTCTGTGAGAAGAGACTACAGCTCATAGAGGAAT
TGTGTGGGAAACTCCCCGTCCAGGACCCAGTAAGGGACACATGTGGAGCCTGTCACACAGCTCTCAAAGA
GCTCAAAGCTTCCATTGACAACACTTACACAATGCTCGTGGATGACCCAGACAAGTGGAAGGACTATACC
AGCAGGTTTTCTGAGTTCTCCTCTTGGGTGTCTGCAAAGAAGGCATGTTTGAAGAAAATCAAGGATGAGC
CCATTGACACTGGCAACCACGACGAGGTTAAGCATATGGTGGATGAGATCAGAAATGACATTACCAAGAA
AGGAGAGAGTCTCAGCTGGCTGAAATCCAGGCTTAAGTATTTGATTGATATTTCTTCAGAGAATGAAGCC
CAAAAGCGAGGAGATGAGCTGGCAGAATTATCAAGCTCTTTCAAGGCTCTTGTGGCATTACTGTCAGAGG
TCGAAAAGCTTCTAAGCAACTTTGGAGAATGCGTTCAGTACAAGGAAATCGTGAAAAGTTCCCTTGAGGG
GTTGATATCGGGCCCCCAAGAAAGCAAGGAAGAGGCTGAGATGATCCTGGACTCGAAAAATCTGCTTGAG
GCACAGCAGTTGCTCCTGCATCACCAGCAAAAGACAAAGATGATCTCAGCAAAGAAGAGAGACCTCCAGG
AGCAGATGGAGCAGGCACAGCAGGGTGGGCAGGCAGGCCCAGGTCAAGAGGAGCTGCGGAAGCTGGAGAG
CACCCTCACAGGCCTGGAGCAGAGCAGGGAGAGGCAGGAACGTCGCATCCAGGTCAGCTTGAGGAAATGG
GAGCGGTTTGAGACAAACAAGGAGACGGTGGTCAGATATCTTTTCCAGACAGGTTCCAGCCACGAACGCT
TCTTGAGCTTCAGCAGTTTGGAAAGCCTGTCATCAGAGTTGGAGCAGACAAAGGAGTTTTCTAAGAGGAC
GGAAAGCATTGCCACCCAGGCTGAGAACCTGGTCAAGGAAGCCGCAGAGTTGCCGCTGGGGCCCAGGAAT
AAGCGTGTGCTTCAGCGGCAGGCCAAGTCAATCAAGGAGCAGGTCACAACCCTGGAAGACACGCTTGAAG
AAGAGTATGTGCTTCACCACTTCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_153399
Insert Size 4296 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153399.2, NP_700448.2
RefSeq Size 6982 bp
RefSeq ORF 4296 bp
Locus ID 64009
Gene Summary Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) differs in both UTRs and has multiple differences in the coding region compared to variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (1) is shorter and has distinct N- and C-termini compared to isoform 4.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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