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Dcaf1 (NM_001015507) Mouse Untagged Clone
CAT＃: MC224421
Vprbp (untagged) - Mouse Vpr (HIV-1) binding protein (Vprbp), (10ug)
CNY 18,240.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI447437; B930007L02Rik; mKIAA0800; Vprbp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224421 representing NM_001015507
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAGTGGGAAAAGG ACCATGGCAGTGGCCAGGATATGGTACCAATCCTTACCAGAATGTCTGAATTAATTGAAAAAGAAACTGA AGAATATCGTAAAGGAGATCCAGATCCATTTGATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATG CTGGGCCATTTGCTGAGAATACTCTTCAAGAATGATGATTTCATGAATGCGCTGGTAAATGCATATGTGA TGACAAGTAGAGAGCCCCCTTTAAATACTGCAGCTTGCAGACTGCTGCTGGACATCATGCCGGGCCTAGA AACCGCAGTTGTCTTTCAAGAAAAGGAAGGAATTGTTGAAAATCTTTTCAAATGGGCCCGAGAGGCTGAT CAGCCACTTAGGACATATTCTACTGGACTGCTGGGAGGTGCTATGGAAAATCAAGATATTGCTGCCAACT ACAGAGATGAAAATTCACAGTTGGTGGCAATAGTGCTTAGAAGACTGAGGGAGCTCCAGCTTCAAGAAGT GGCTTTGCGGCAGGACAGTAAGCGTCCTAGTCCACGGAAGCTCTCTTCGGAACCCCTTTTGCCTCTGGAT GAGGAAGCTGTGGATATGGACTATGGTGACATGGCAGTGGATGTTGTGGATGGAGAACAAGAATCTTCTA GAGATATGGAGATCTCCTTTCGTCTTGATTCAAGTCACAAGACCAGTAGTAGAGTGAACTCAGCAACTAA ACCTGAGGAAGGAGGATTAAAGAAAAACAAGTCAGCAAAGCATGGTGACAGAGAGAACTTTAGGAAGGCC AAGCAGAAGTTGGGCTTCTCATCTTCTGACCCAGATCGTGTGTTTGTTGAGCTGTCTAATAGCAGCTGGT CAGAAATGTCCCCCTGGGTGATCGGCACCAATTACACTCTCTATCCTATGACTCCTGCTATTGAGCAGAG ACTTATTCTTCAGTATTTGACCCCTTTAGGAGAGTATCAGGAGTTACTTCCCATATTCATGCAACTTGGA TCAAGAGAATTGATGATGTTTTATATTGATCTGAAACAGACGAATGATGTCCTGCTTACTTTTGAGGCAC TCAAGCACCTAGCATCTCTCCTGCTTCATAACAAATTTGCCACAGAATTTGTTGCACATGGTGGAGTACA GAAATTACTGGAAATTCCTCGTCCTTCTATGGCTGCAACTGGTGTGTCCATGTGCTTGTATTACCTGTCC TACAATCAGGATGCTATGGAAAGAGTTTGCATGCATCCTCACAATGTTCTATCTGATGTGGTGAACTATA CCCTGTGGTTAATGGAATGTTCTCATGCTTCAGGATGTTGCCATGCTACTATGTTTTTCTCAATTTGTTT CTCATTCCGGGCTGTTTTGGAGCTCTTTGACCGTTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGT ACTTTGGAGATTTTAAATTTGGAAGACCAGGGTGCACTTTTGAGTGATGATGAAATATTTGCTAGTCGCC AAACAGGAAAACATACCTGCATGGCTCTGCGCAAGTATTTTGAGGCTCATCTGGCCATTAAATTGGAACA AGTCAAGCAGTCACTTCAGAGGACTGAGGGTGGTATTCTTGTTCACCCCCAGCCTCCATACAAGGCATGC TCATATACTCATGAGCAGATTGTAGAAATGATGGAATTTTTGATAGAGTATGGCCCAGCTCAGCTATATT GGGAACCTGCTGAAGTCTTCCTCAAGCTTTCCTGTGTGCAGCTTTTACTACAGCTTATTTCCATCGCCTG CAATTGGAAGACCTATTATGCAAGGAATGATACTGTGCGTTTTGCTTTGGATGTCCTTGCTATTCTCACT GTGGTGCCAAAAATCCAGCTCCAGTTGGCGGAGTCAGTGGATGTACTGGACGAGGCCGGCTCTGCTGTCT CCACTGTAGGTATCAGCATTATTTTGGGCGTGGCTGAGGGTGAGTTCTTTATCCATGATGCTGAAATTCA GAAGTCAGCACTTCAAATTATCATCAATTGTGTATGTGGTCCAGATAACCGAATATCCAGTATTGGCAAA TTTATTTCTGGAACTCCTCGGAGAAAGCTGTCTCAGACTCCCAAGAGTAGTGAGCATACTCTGGCCAAGA TGTGGAATGTGGTCCAGTCCAACAATGGCATCAAGGTGCTTCTGTCCCTGTTGTCCATCAAGATGCCCAT TACAGACGCAGACCAGATCCGGGCCCTGGCCTGCAAAGCCCTTGTAGGCCTGTCTCGAAGTAGCACTGTT CGACAGATCATCAGTAAACTTCCCCTTTTTAGTAGCTGCCAGATTCAACAGCTGATGAAAGAGCCTGTGC TGCAGGATAAGCGTAGTGACCATGTCAAGTTCTGCAAATACGCTGCTGAGCTCATTGAGCGTGTATCAGG AAAGCCTCTTCTCATTGGCACTGATGTGTCTCTCGCACGACTGCAGAAAGCAGATGTTGTTGCTCAGTCG AGGATCTCCTTCCCTGAGAAAGAGCTGCTCCTGTTGATACGGAACCATCTCATTTCTAAAGGGCTTGGAG AGACAGCAACTGTGCTGACGAGAGAGGCTGACCTGCCCATGACTGCTGCCTCACATTCTTCTGCCTTCAC CCCAGTGACTGCTGCTGCTTCTCCTGTCTCTCTTCCCCGGACCCCTCGGATTGCCAACGGCATTGCAAGT CGACTGGGCAGCCATGCTACTGTGGGTGCTTCTGCCCCTTCTGCTCCCCCTGCCCATCCTCCACCACGGC CCCCCCAGGGTTCATTGCCTTTGCCTGGCCCATCATATGCAGGCAATTCCCCTTTGATTGGTAGGATCAG TTTTATCAGAGAGAGGCCATCACCGTGCAATGGCAGGAAAATTCGAGTGTTGCGGCAGAAGTCGGACCAT GGTGCATACAGCCAGAGCCCTGCCATAAAAAAACAATTGGATAGACATCTTCCTTCCCCACCTACGCTGG ACAGTATCATCACAGAGTATCTTAGAGAGCAACATGCTCGATGCAAGAACCCCGTTGCCACCTGTCCTCC TTTTTCTCTCTTTACTCCTCATCAGTGTCCTGAACCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACC TCAAGGCTAAACCGCAGGGCATCATTTCCAAAATATGGAGGGGTAGATGGCGGATGCTTTGATAGGCACC TTATCTTTAGCAGATTTCGTCCGATTTCAGTATTCCGGGAAGCTAATGAAGATGAAAGTGGCTTCACCTG TTGTGCATTCTCAGCCCGGGAGCGGTTCCTGATGCTTGGCACTTGTACAGGGCAGCTGAAGCTCTACAAT GTGTTTAGTGGACAGGAAGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACACCTTGAACCGTCAA GGGATGGGTCCTTACTTCTGACATCTGCCACTTGGAGCCAGCCTTTGTCTGCACTTTGGGGAATGAAATC AGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTATGTTGAGTTCAGTAAGCACTCTCAGGATCGG GTCATAGGCACAAAAGGAGACATTGCCCATATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGT TTAACCCAGATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTT AAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAACATGAAT ATCAGTGGTGTTTTCCATCCAAATGGGCTGGAAGTCATCATCAATACAGAGATTTGGGACCTTCGGACTT TCCATCTTTTACACACTGTTCCTGCTCTGGATCAATGCCGTGTTGTTTTTAACCACACTGGGACAGTGAT GTATGGAGCAATGTTGCAGGCAGATGATGAAGATGACTTACTGGAAGAGAGGATGAAAAGCCCTTTTGGG TCATCCTTTCGAACATTCAACGCAACTGATTATAAACCTATAGCAACTATTGATGTGAAACGAAACATCT TTGACCTGTGCACAGACACCAAAGACTGTTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCACT GAATATGGACACAGTGTGCAGGCTCTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAG GAGGACCAGCAGACAGCTCGGACAACTCTGATTTGGAAGATGACATCATCTTATCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001015507 |
| Insert Size | 4260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001015507.2, NP_001015507.1 |
| RefSeq Size | 5392 bp |
| RefSeq ORF | 4260 bp |
| Locus ID | 321006 |
| UniProt ID | Q80TR8 |
| Gene Summary | Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex. The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition. Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors. Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211979 | Vprbp (tGFP-tagged) - Mouse Vpr (HIV-1) binding protein (Vprbp) |
CNY 15,580.00 |
|
| MR211979 | Vprbp (Myc-DDK-tagged) - Mouse Vpr (HIV-1) binding protein (Vprbp) |
CNY 9,712.00 |
|
| MR211979L3 | Lenti ORF clone of Vprbp (Myc-DDK-tagged) - Mouse Vpr (HIV-1) binding protein (Vprbp) |
CNY 16,060.00 |
|
| MR211979L4 | Lenti ORF clone of Vprbp (mGFP-tagged) - Mouse Vpr (HIV-1) binding protein (Vprbp) |
CNY 16,060.00 |
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