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Med23 (NM_027347) Mouse Untagged Clone
CAT＃: MC224351
Med23 (untagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2, (10ug)
CNY 17,580.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 130kDa; 3000002A17Rik; Crsp3; ESTM7; mKIAA1216; Sur2; X83317 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224351 representing NM_027347
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCGATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG CCTTCCCAGGAATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTAATTAGCTGCTTGGCGGCCTT CCGGCAGTTTTGGAGTGGACTTTCTCAGGAGTCTCATGAACAGTGTGTTCAGTGGATTGTTAAATTTATT CATGGCCAACACAGCCCCAAAAGAATTTCTTTCCTCTATGACTGCTTAGCAATGGCAGTTGAGACTGGTC TCCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACTCTCTTGAATGGGAAAGAACACAGCT TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTACAAGGGTGTTCGAGACCTT CTCAAAGCGATTTTGGAGAAAATCTTGACAATCCCAAATACAGTGAGCTCTGCTGTTGTCCAGCAGCTTC TGGCAGCAAGAGAGGTTATAGCTTATATCTTAGAACGAAATGCCTGCCTATTGCCGGCCTACTTTGCAGT CACAGAGATCAGAAAACTATATCCTGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC TTTGTGGATACCTTCAGGCCCACAGCAAGGATTAACTCCATTTGTGGCCGATGCAGTCTTCTGCCAGTTG TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTGCGATTTCCTTTGAAAGG CCTTTTGCCATATGATAAGGATCTGTTTGAACCGCAGACTGCTTTGTTGAGATATGTACTGGAGCAACCT TATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGG AGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCAGAGACCGAGGAGAAGTTTGACGATGG GGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCC AGTTTCCCACATATGGTCCTCTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACC ATCTGATGTGGGTGCTGCTGCAGTTCATTTCTGGAAGTATTCAGAAAAATGCCCTCGCTGACTTCCTCCC TGTCATGAAGCTCTTTGACCTGCTGTACCCGGAGAAGGAGTGTATCCCAGTTCCTGATATTAACAAACCC CAGTCAACGCACGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGGCGACT CCACGCTCCAGATCCCAATACCCCATTCCCTGAAGCTGCACCATGAGTTCCTCCAGCAGAGTCTGAGAAA TAAAAGCTTACAGATGAATGACTATAAGATTGCCCTGCTATGTAACGCGTATTCCACAAACTCAGAGTGT TTTACGTTACCTATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGAGTGCCTCTCCCTG GAACGAGCTGTTTGGCCTCAGCATCAGTGACTCCCTTACCGATGAACCTCCTGGATTCACTGACGGTGCA TGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACGAAATCCAGTGTT GCGCTGGCTCCAGCCCTAGTGGAAACTTACAGCCGTTTACTGGTCTACATGGAAATTGAGTCTTTGGGCA TCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCACGCCTGGGGCATCCTGCACACACT GCTGGAGATGTTCAGCCACCGAATGCACCACATTCAGCCCCACTACCGAGTTCAGCTCCTGAGCCATCTC CACACACTGGCTGCAGTCGCACAGACCAACCAGAACCAGCTCCATCTGTGTGTGGAGAGCACTGCACTGA GGCTCATCACAGCCCTGGGAAGCTCAGAGGTCCAGCCGCAGTTCACGCGTTTCCTCAATGATCCCAAAAC AGTGCTGTCCGCCGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTAGCCAGAGCAACCCACGTC ACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGA ACTTTACTCCTCATAACTGGGCTTCACATACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCTTTCTTCAA GCAAAATAACGTGCCCCAGGAAAGCCGTTTTAATCTGAAGAAGAATGTGGAAGAAGAGTACAGGAAGTGG AAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCTGTGCAGGGCTTCCCTCCACTCTTCCTCT GTCTCCTCTGGAAGATGCTCCTGGAGACGGATCATATTAGTCAGATCGGCTACAAGGTCCTGGAGAGGAT TGGGGCCAGGGCCCTGGTGGCCCATGTGAGAACATTTGCAGATTTCCTGGTGTATGAGTTCTCTACGTCA GCAGGAGGTCAGCAACTCAATAAGTGCATTGAAATTCTCAACGACATGGTGTGGAAGTACAACATTGTCA CGCTGGACAGACTCATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCCCAGGTTTGTTATTT CATAATTCAGTTGCTGTTGCTCAAACCAAATGACTTCAGAAACCGAGTAAGTGACTTTGTGAAGGAGAAT TCCCCAGAGCACTGGCTCCAGAGTGACTGGCACACCAAGCACATGAGCTACCACAAGAAATATCCCGAGA AGCTGTATTTTGAAGGCCTGGCAGAGCAGGTTGACCCTCCTGTACCAATCCAGTCCCCTTATCTGCCCAT CTACTTTGGAAATGTGTGTCTCCGGTTCCTTCCAGTATTTGATATAGTAATCCACAGGTTTTTAGAGTTG CTTCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTATATAAATTCCATGACCGGC CAGTGACTTATCTGTATAATACTTTGCACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACG AAAACTTGTCCATGCAATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGATACC TACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCGGAGGACTCCTACTACTGCAAGC TGATTGGCCGACTGGTAGACACCATGGCGGGCAAGTCTCCAGGACCCTTCCCAAACTGTGACTGGAGATT CAATGAGTTTCCCAACCCCGCTGCCCACGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCGGTT CCAGGCAAAGATGTGGGGAATGCCCTTCTCAATGTCGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGA ACATTACAGCGTGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGAGCCGTACTGGATTGTGCT TCATGACCGAATTGTGAATGTGATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTCGGTTACCCG TTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCGGAGATGAGCTGCAGCTATACGTTAGCTC TAGCCCATGCCGTGTGGCACCACTCCAGCATCGGGCAGCTCTCGCTTATCCCAAAGTTTCTCACTGAGGC GCTTCTCCCGGTGGTGAAGACTGAGTTCCAGTTGCTCTACGTGTACCATCTTGTGGGGCCATTTCTACAG AGGTTCCAACAGGAGAGAACCCGGTGCATGATTGAGATTGGCGTGGCATTTTATGACATGCTGTTGAATG TAGACCAGTGCAGCACGCACTTAAATTACATGGACCCCATCTGCGACTTCCTGTATCATATGAAGTATAT GTTTACTGGTGACAGTGTGAAAGAGCAAGTAGAGAAGATTATCTGTAATTTAAAGCCGGCTTTAAAGCTT CGTCTTCGATTCATCACGCACATTAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAACAGCGGGT CTCCAGCACCTCAGTCTAACCAGGTGCCAGCATCTCTGCCGGTGACTCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_027347 |
| Insert Size | 4113 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027347.3, NP_081623.3 |
| RefSeq Size | 4928 bp |
| RefSeq ORF | 4113 bp |
| Locus ID | 70208 |
| UniProt ID | Q80YQ2 |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211949 | Med23 (tGFP-tagged) - Mouse cofactor required for Sp1 transcriptional activation, subunit 3 (Crsp3) |
CNY 11,880.00 |
|
| MR211949 | Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 9,376.00 |
|
| MR211949L3 | Lenti ORF clone of Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 12,730.00 |
|
| MR211949L4 | Lenti ORF clone of Med23 (mGFP-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 12,730.00 |
