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Msh6 (NM_010830) Mouse Untagged Clone
CAT＃: MC224318
Msh6 (untagged) - Mouse mutS homolog 6 (E. coli) (Msh6), (10ug)
CNY 17,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AU044881; AW550279; GTBP; Gtmbp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224318 representing NM_010830
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGACAAAGCACCTTGTACAGCTTCTTCCCCAAGTCCCCGGCACTCGGCGACACCAAGAAGGCCG CGGCCGAGGCCTCACGCCAAGGTGCCGCTGCCTCTGGAGCCTCCGCTTCCCGCGGCGGGGATGCGGCCTG GAGCGAGGCTGAACCCGGGTCCCGGTCCGCGGCGGTATCCGCCTCGTCGCCGGAGGCAAAGGATCTCAAC GGAGGGCTGCGGAGGGCCTCCTCCTCCGCGCAGGCCGTGCCTCCCAGTTCTTGTGACTTCTCACCAGGTG ATTTGGTTTGGGCTAAGATGGAAGGTTACCCCTGGTGGCCTTGCCTAGTTTATAATCATCCCTTTGATGG AACGTTCATCCGGAAGAAAGGGAAATCTGTCCGTGTTCATGTACAGTTCTTTGATGACAGCCCAACAAGG GGCTGGGTTAGCAAAAGGATGTTAAAGCCATATACAGGTTCAAAGTCAAAGGAAGCCCAAAAGGGAGGTC ATTTCTACAGTTCAAAGTCTGAGATACTCAGAGCAATGCAACGTGCAGATGAGGCCTTAAGTAAAGACAC GGCTGAGAGGCTGCAGCTGGCAGTGTGTGATGAGCCTTCAGAGCCGGAGGAGGAGGAGGAGACAGAGGTG CATGAGGCTTATTTATCTGATAAGAGTGAAGAGGATAATTACAACGAGAGTGAAGAAGAAGCACAGCCCA GTGTTCAGGGGCCTAGGCGAAGCAGCCGGCAAGTCAAGAAGCGACGGGTCATCTCAGACTCGGAGAGTGA CATTGGTGGCTCTGATGTAGAATTCAAGCCAGACACTAAGCAGGAAGGAAGCAGTGATGACGCGAGCAGT GGAGTTGGGGACAGCGATAGTGAGGACCTAGGCACCTTTGGCAAAGGCGCTCCAAAGCGGAAGAGAGCCA TGGTTGCTCAGGGTGGACTTAGAAGGAAAAGTCTGAAGAAGGAAACAGGCTCAGCCAAACGAGCAACTCC CATTCTCTCAGAAACCAAGAGTACCTTGAGCGCTTTTTCTGCCCCTCAGAATTCTGAATCCCAGACCCAC GTCAGTGGAGGAGGTAACGACAGTAGTGGACCCACTGTTTGGTATCACGAAACTTTAGAATGGCTTAAGC CAGAAAAGAGAAGAGATGAGCACAGGAGACGCCCCGATCACCCTGAATTTAACCCCACCACACTGTATGT GCCTGAAGAGTTCCTCAATTCTTGTACCCCAGGGATGAGGAAGTGGTGGCAGCTCAAGTCTCAGAACTTT GACCTCGTCATCTTTTATAAGGTGGGGAAGTTTTATGAGTTGTATCACATGGATGCTGTTATTGGAGTCA GTGAGCTGGGGCTGATATTCATGAAGGGCAACTGGGCCCATTCTGGTTTTCCAGAGATTGCGTTTGGCCG GTTCTCGGATTCCTTGGTGCAGAAGGGCTATAAGGTAGCACGAGTGGAGCAGACCGAGACTCCAGAAATG ATGGAGGCGCGATGTCGGAAAATGGCACACGTGTCCAAGTTTGATAGAGTGGTGAGAAGGGAGATTTGCA GGATCATTACCAAGGGCACACAGACCTACAGTGTGCTGGATGGTGATCCCTCTGAGAACTACAGTAGGTA TCTTCTTAGCCTCAAAGAGAAGGAGGAAGAGACTTCTGGTCACACTCGTGTGTATGGTGTTTGCTTTGTG GACACTTCACTGGGCAAGTTTTTCATCGGCCAGTTTTCGGATGACCGCCACTGTTCCAGATTTAGGACGC TAGTGGCTCACTATCCTCCAGTACAAATTTTGTTCGAGAAAGGAAATCTCTCAACAGAGACCAAAACTGT CCTAAAGGGTTCACTGTCATCTTGTCTTCAGGAAGGTCTCATACCAGGTTCCCAATTTTGGGATGCCACT AAGACCTTGCGAACACTCCTTGAAGGAGGGTACTTTACTGGAAATGGAGACAGTAGTACAGTGCTGCCTC TGGTGCTTAAAGGAATGACCTCGGAGTCAGATTCTGTTGGGTTGACACCAGGCGAGGAGAGTGAATTGGC TCTCTCTGCTCTAGGTGGTATTGTTTTCTACCTCAAAAAATGCCTGATTGATCAGGAGCTTCTGTCAATG GCTAATTTTGAAGAGTATTTCCCTTTGGATTCTGACACGGTCAGCACAGTAAAACCTGGGGCTGTCTTTA CTAAAGCCAGTCAGCGGATGGTGCTAGACGCAGTGACATTAAACAACCTGGAGATTTTCCTGAATGGGAC AAATGGCTCTACTGAAGGGACCCTGCTAGAGAGATTGGATACTTGCCATACGCCCTTTGGCAAGCGGCTC TTAAAACAGTGGCTCTGTGCCCCCCTCTGCAGCCCTTCTGCCATCAGTGACCGTCTAGATGCGGTGGAAG ATCTGATGGCCGTGCCTGACAAGGTCACTGAAGTTGCAGACCTCTTAAAGAAGCTGCCAGACCTTGAGAG GCTACTGAGTAAGATTCATAATGTTGGGTCTCCCCTGAAGAGCCAGAACCATCCAGACAGCAGGGCTATA ATGTACGAAGAAACGACATACAGCAAAAAGAAGATCATTGATTTTCTCTCTGCTCTAGAAGGATTCAAAG TAATGTGTAAAGTCTCAGGGCTTCTGGAGGAGGTCGCGGGTGGTTTTACGTCCAAAACCCTCAAGCAGGT TGTTACTCTGCAGTCAAAAAGTCCTAAAGGCCGCTTTCCTGACCTGACTGCAGAACTGCAGCGATGGGAC ACAGCCTTTGACCATGAGAAGGCTCGGAAGACCGGACTCATCACTCCAAAAGCAGGGTTTGACTCTGATT ATGACCAAGCACTTGCTGACATCAGAGAGAATGAACAGAGCCTCCTGGAGTACTTAGACAAACAGCGCAG TCGGCTTGGCTGTAAGAGCATAGTCTACTGGGGAATCGGTAGGAACCGTTACCAGTTAGAGATTCCAGAG AATTTCGCTACCCGTAATCTACCTGAAGAATATGAGCTGAAATCTACTAAAAAAGGCTGTAAACGATACT GGACCAAAACGATTGAGAAGAAATTAGCTAATCTTATTAATGCTGAAGAACGCAGGGACACATCCTTAAA GGACTGCATGCGGCGCTTGTTCTGTAACTTCGACAAGAACCACAAGGACTGGCAGTCTGCTGTAGAGTGC ATTGCAGTGTTGGATGTCTTACTGTGCCTGGCTAACTACAGTCAAGGAGGAGATGGTCCTATGTGTCGCC CAGAAATTGTGTTACCAGGAGAAGACACACATCCCTTCTTAGAGTTTAAAGGGTCACGACATCCCTGCAT TACAAAGACTTTTTTTGGAGACGATTTTATTCCTAACGACATTCTAATAGGCTGCGAGGAAGAAGCAGAG GAACATGGCAAAGCCTATTGTGTGCTTGTGACTGGACCAAATATGGGGGGCAAGTCTACACTCATAAGAC AGGCTGGTCTGTTGGCTGTGATGGCCCAGCTGGGTTGTTACGTACCTGCTGAGAAGTGCAGGCTCACACC AGTTGACAGAGTGTTTACTAGGCTTGGTGCCTCAGATAGAATAATGTCAGGAGAAAGCACATTTTTTGTT GAGTTGAGTGAAACTGCTAGCATACTCAGGCATGCAACAGCACATTCTTTGGTACTTGTGGATGAATTAG GAAGAGGCACCGCAACTTTTGATGGGACAGCGATAGCCAATGCGGTTGTTAAAGAACTTGCTGAAACCAT AAAGTGTCGAACACTGTTTTCTACACACTATCATTCATTAGTAGAAGATTATTCTAAAAGTGTTTGTGTG CGCCTAGGACATATGGCATGCATGGTAGAAAATGAATGTGAGGACCCCAGCCAGGAGACTATTACTTTCC TCTATAAATTCATTAAGGGAGCTTGTCCTAAGAGCTATGGCTTTAATGCAGCAAGGCTTGCTAATCTCCC GGAGGAGGTTATTCAGAAGGGACACAGAAAGGCAAGAGAATTTGAGAGGATGAATCAGTCATTACAGCTA TTTCGGGAAGTTTGTCTGGCTACTGAAAAGCCGACTATAAACGGTGAAGCTATCCATAGGCTGCTGGCCT TGATTAACGGATTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010830 |
| Insert Size | 4077 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010830.2, NP_034960.1 |
| RefSeq Size | 4288 bp |
| RefSeq ORF | 4077 bp |
| Locus ID | 17688 |
| UniProt ID | P54276 |
| Gene Summary | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211944 | Msh6 (tGFP-tagged) - Mouse mutS homolog 6 (E. coli) (Msh6) |
CNY 13,110.00 |
|
| MR211944 | Msh6 (Myc-DDK-tagged) - Mouse mutS homolog 6 (E. coli) (Msh6) |
CNY 9,296.00 |
|
| MR211944L3 | Lenti ORF clone of Msh6 (Myc-DDK-tagged) - Mouse mutS homolog 6 (E. coli) (Msh6) |
CNY 13,780.00 |
|
| MR211944L4 | Lenti ORF clone of Msh6 (mGFP-tagged) - Mouse mutS homolog 6 (E. coli) (Msh6) |
CNY 13,780.00 |
