Clasp2 (NM_001081960) Mouse Untagged Clone

CAT#: MC224170

Clasp2 (untagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 2, (10ug)



  "NM_001081960" in other vectors (4)

CNY 16,630.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224170 representing NM_001081960
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCGGCTGATTTGCAAACGGATCTGTGATTATAAAAGCTTTGATGATGAGGAATCAGTGGATGGAA
ATAGGCCGTCGTCAGCTGCTTCAGCCTTCAAGGTTCCTGCACCTAAAACACCTGGGAATCCTGTCAGCAG
TGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGAGGTCCTTCTAAAGAAGGAGGGGCTGGAGCA
GTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTCCTTCTGTTCAGATCTATTCTAGTCGAGAAC
TTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGATGACAAACATGACTGGGACCAGCGTGCCAA
TGCGCTTAAGAAAATCAGATCACTGCTTGTTGCTGGAGCCGCACAGTATGATTGCTTTTTCCAGCACCTA
CGTTTGTTGGATGGAGCGCTTAAGCTGTCAGCTAAGGACCTCAGATCCCAGGTGGTCAGGGAAGCTTGCA
TCACTGTTGCTCACCTTTCAACAGTCTTGGGAAACAAATTTGATCACGGCGCTGAAGCCATCGTCCCTAC
GCTTTTTAATCTTGTCCCCAATAGTGCCAAAGTCATGGCGACCTCTGGATGTGCAGCAATCAGATTTATT
ATTCGGCATACCCATGTACCTAGACTTATTCCTTTAATAACAAGCAACTGCACATCAAAGTCAGTTCCTG
TAAGGAGACGTTCATTTGAATTTTTAGATTTGCTGCTGCAAGAATGGCAGACTCATTCACTGGAAAGACA
TGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGCATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCA
AGGAAGACATACATGGGCCTTAGGAACCACTTTCCTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGC
CATCATATCAGAAAAGTCTTCAAACTTACTTAAAGAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGA
CAGGTCCTCATCCAGCTCACAAGAAAGTCTCAATCGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCT
TCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCAGCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTT
CTCGAAGTGACATTGATGTGAATGCGGCAGCTGGTGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCG
AAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCAGGCTCCTATGCATCACTAGAGGATACTTCTGACAAG
ATGGATGGAACAGCATCTGATGATGGTCGGGTGAGAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAA
ATGCCAAGACCGACTCTAGAGGGAGAAGCCGGACAAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATC
TGGGTCTCCAGGAAGAGTTCTAACCACAACAGCCCTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTG
GTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGATCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCC
CATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTATTCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGA
AGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCGGTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGG
ATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCAGTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGG
AGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGACATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTA
TGAATCATATGGAATGCACTCAGATGATGATGCCAACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCC
TATAGCTCTCGAAATGGTAGTATTCCTACCTACATGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACA
GATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGAAGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCA
GAGAACGCTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCAGATCCTCAT
GGCAAGAGAGTGTTCAGCATGTTCTTGGAGACTCTAGTAGATTTCATACAAGTCCACAAAGATGATCTTC
AAGATTGGTTGTTTGTTCTGCTGACACAGCTGCTGAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCA
GGCAAAAGTTCAGAAAGCCCTTGATATTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTA
ATGAGATTTACAGTTGACCAGACCCAAACGCCAAGCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAG
AAACTCTGGCAAAGCAGATGGACCCAAGAGATTTTACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCG
GGTCATCACTTGGACGACAGAGCCCAAAAGCTCTGATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCT
TTATTTGAACTCAATACCCCAGAGTTTACAATGTTACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTG
CTACTAAACTTCTTCACAATCACCTCCGGAACACTGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTT
GACGAGACCAACACCTCGGTCACCAGCCAACTGGTCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAG
AATACGTTATCTCCAAGTGCATTTGATTACGATACAGAGAACATGAATTCTGAAGACATTTATAGCTCCC
TTAGAGGCGTCACTGAGGCAATCCAGAATTTCAGCTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAG
GAGGGACCCTAAAAAGGAGGATGGTGACACAATATGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGA
GGTGATGCTGCTGACGGCAGCCAGCCAGCTCTGGATAATAAAGCATCGTTGCTCCACTCAATGCCACTCC
ACTCCTCTCCACGCTCCCGTGACTATAACCCATATAACTACTCAGATAGCATCAGTCCTTTCAACAAGTC
TGCCCTCAAGGAAGCCATGTTTGATGATGACGCCGACCAATTTCCTGATGATCTTTCCTTAGACCATTCT
GACCTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAATCATAATGAACGTATAGAAGAAAGAAAAATTGCCC
TGTATGAACTCATGAAGCTAACCCAGGAAGAATCTTTCAGTGTTTGGGATGAACACTTCAAAACAATATT
ATTGTTATTGCTTGAGACCCTTGGGGATAAAGAGCCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAA
GAAATCTTAAGGCATCAACCAGCAAGATTCAAAAACTATGCAGAACTAACTGTCATGAAAACACTGGAAG
CACATAAAGATCCTCACAAAGAGGTGGTGAGATCTGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCAT
TAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCCATCATACAAACCGCTGACTACCCTATTAATCTGGCT
GCAATCAAAATGCAAACAAAAGTGATAGAGAGAGTATCCAAGGAGACCCTTAACATGCTCTTACCAGAGA
TCATGCCGGGTCTAATACAGGGTTATGATAATTCAGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCT
GGTGGCTGTCCATGCAGTGATTGGTGATGAACTAAAGCCACATCTCAGTCAACTCACTGGTAGTAAAATG
AAGCTGCTGAACCTTTACATCAAGCGTGCACAGACGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTT
CTGGACAGAGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081960
Insert Size 3864 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081960.1, NP_001075429.1
RefSeq Size 5627 bp
RefSeq ORF 3864 bp
Locus ID 76499
Gene Summary Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 3. It encodes isoform b, which has a shorter and distinct N-terminus, compared to isoform c.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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