Clasp2 (NM_029633) Mouse Untagged Clone

CAT#: MC224148

Clasp2 (untagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1, (10ug)



  "NM_029633" in other vectors (4)

CNY 16,530.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224148 representing NM_029633
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCGGCTGATTTGCAAACGGATCTGTGATTATAAAAGCTTTGATGATGAGGAATCAGTGGATGGAA
ATAGGCCGTCGTCAGCTGCTTCAGCCTTCAAGGTTCCTGCACCTAAAACACCTGGGAATCCTGTCAGCAG
TGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGAGGTCCTTCTAAAGAAGGAGGGGCTGGAGCA
GTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTCCTTCTGTTCAGATCTATTCTAGTCGAGAAC
TTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGATGACAAACATGACTGGGACCAGCGTGCCAA
TGCGCTTAAGAAAATCAGATCACTGCTTGTTGCTGGAGCCGCACAGTATGATTGCTTTTTCCAGCACCTA
CGTTTGTTGGATGGAGCGCTTAAGCTGTCAGCTAAGGACCTCAGATCCCAGGTGGTCAGGGAAGCTTGCA
TCACTGTTGCTCACCTTTCAACAGTCTTGGGAAACAAATTTGATCACGGCGCTGAAGCCATCGTCCCTAC
GCTTTTTAATCTTGTCCCCAATAGTGCCAAAGTCATGGCGACCTCTGGATGTGCAGCAATCAGATTTATT
ATTCGGCATACCCATGTACCTAGACTTATTCCTTTAATAACAAGCAACTGCACATCAAAGTCAGTTCCTG
TAAGGAGACGTTCATTTGAATTTTTAGATTTGCTGCTGCAAGAATGGCAGACTCATTCACTGGAAAGACA
TGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGCATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCA
AGGAAGACATACATGGGCCTTAGGAACCACTTTCCTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGC
CATCATATCAGAAAAGTCTTCAAACTTACTTAAAGAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGA
CAGGTCCTCATCCAGCTCACAAGAAAGTCTCAATCGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCT
TCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCAGCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTT
CTCGAAGTGACATTGATGTGAATGCGGCAGCTGGTGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCG
AAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCAGGCTCCTATGCATCACTAGAGGATACTTCTGACAAG
ATGGATGGAACAGCATCTGATGATGGTCGGGTGAGAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAA
ATGCCAAGACCGACTCTAGAGGGAGAAGCCGGACAAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATC
TGGGTCTCCAGGAAGAGTTCTAACCACAACAGCCCTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTG
GTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGATCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCC
CATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTATTCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGA
AGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCGGTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGG
ATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCAGTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGG
AGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGACATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTA
TGAATCATATGGAATGCACTCAGATGATGATGCCAACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCC
TATAGCTCTCGAAATGGTAGTATTCCTACCTACATGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACA
GATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGAAGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCA
GAGAACGCTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCAGATCCTCAT
GGCAAGGTGTTCAGCATGTTCTTGGAGACTCTAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAG
ATTGGTTGTTTGTTCTGCTGACACAGCTGCTGAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGC
AAAAGTTCAGAAAGCCCTTGATATTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATG
AGATTTACAGTTGACCAGACCCAAACGCCAAGCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAA
CTCTGGCAAAGCAGATGGACCCAAGAGATTTTACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGT
CATCACTTGGACGACAGAGCCCAAAAGCTCTGATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTA
TTTGAACTCAATACCCCAGAGTTTACAATGTTACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTA
CTAAACTTCTTCACAATCACCTCCGGAACACTGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGAC
GAGACCAACACCTCGGTCACCAGCCAACTGGTCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAAT
ACGTTATCTCCAAGTGCATTTGATTACGATACAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTA
GAGGCGTCACTGAGGCAATCCAGAATTTCAGCTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAG
GGACCCTAAAAAGGAGGATGGTGACACAATATGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGT
GATGCTGCTGACGGCAGCCAGCCAGCTCTGGATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACT
CCTCTCCACGCTCCCGTGACTATAACCCATATAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGC
CCTCAAGGAAGCCATGTTTGATGATGACGCCGACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGAC
CTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGT
ATGAACTCATGAAGCTAACCCAGGAAGAATCTTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATT
GTTATTGCTTGAGACCCTTGGGGATAAAGAGCCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAA
ATCTTAAGGCATCAACCAGCAAGATTCAAAAACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCAC
ATAAAGATCCTCACAAAGAGGTGGTGAGATCTGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAG
TCCAGAGCAGTGCATCAAAGTGCTTTGTCCCATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCA
ATCAAAATGCAAACAAAAGTGATAGAGAGAGTATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCA
TGCCGGGTCTAATACAGGGTTATGATAATTCAGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGT
GGCTGTCCATGCAGTGATTGGTGATGAACTAAAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAG
CTGCTGAACCTTTACATCAAGCGTGCACAGACGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTG
GACAGAGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029633
Insert Size 3861 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_029633.2, NP_083909.2
RefSeq Size 5624 bp
RefSeq ORF 3861 bp
Locus ID 76499
Gene Summary Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' and 3' coding region, and initiates translation at an alternate start codon, compared to variant 3. It encodes isoform a, which is shorter and has a distinct N-terminus, compared to isoform c.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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