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Npc1 (NM_008720) Mouse Untagged Clone
CAT＃: MC224120
Npc1 (untagged) - Mouse Niemann Pick type C1 (Npc1), (10ug)
CNY 16,440.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224120 representing NM_008720
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCGCACCACCCGGCCCTCGGCCTGCTGCTGCTGCTGCTGTGCCCTGCGCAGGTGTTTTCGCAAT CCTGTGTTTGGTATGGAGAGTGTGGAATTGCGACTGGAGATAAGAGGTACAACTGTAAATATTCTGGCCC ACCAAAACCCCTCCCAAAGGACGGCTATGACTTAGTGCAGGAACTCTGTCCAGGACTCTTCTTTGACAAT GTCAGTCTCTGCTGTGACATTCAACAGCTTCAGACGCTGAAGAGTAACCTGCAGCTGCCCCTGCAGTTCC TGTCCAGGTGTCCGTCATGTTTTTATAACCTAATGACCCTGTTTTGTGAGCTAACATGTAGCCCACACCA GAGTCAGTTTCTGAATGTGACAGCAACTGAGGATTATTTTGATCCTAAGACACAGGAGAATAAAACAAAT GTAAAGGAATTAGAGTACTTTGTCGGACAGAGCTTCGCGAATGCGATGTACAATGCCTGCCGTGATGTGG AGGCGCCTTCCAGTAACGAGAAGGCCTTAGGACTCTTGTGTGGGAGGGATGCCCGTGCCTGCAATGCCAC CAACTGGATTGAGTACATGTTCAATAAAGACAACGGACAAGCGCCATTTACCATCATTCCTGTGTTTTCA GATCTTTCAATCCTTGGGATGGAGCCCATGAGAAATGCCACCAAAGGCTGCAATGAGTCTGTAGATGAGG TCACGGGGCCATGTAGCTGCCAGGACTGCTCCATCGTCTGCGGCCCCAAGCCCCAGCCCCCACCCCCTCC TATGCCCTGGAGGATCTGGGGCTTGGATGCCATGTATGTCATCATGTGGGTCACCTACGTGGCATTTCTG TTTGTGTTTTTTGGAGCACTGTTGGCAGTGTGGTGCCACAGAAGGCGGTACTTTGTGTCTGAGTACACTC CCATTGACAGTAACATCGCCTTTTCTGTGAATAGCAGTGACAAAGGGGAAGCCTCATGCTGTGACCCACT TGGTGCAGCATTTGATGACTGTCTGAGGCGCATGTTCACAAAGTGGGGGGCTTTCTGTGTCCGAAATCCC ACCTGCATCATTTTCTTCTCATTGGCCTTCATCACTGTGTGCTCTTCTGGCCTGGTATTTGTCCAGGTCA CCACCAATCCTGTAGAGCTCTGGTCAGCCCCTCACAGTCAGGCCCGCTTGGAAAAGGAGTACTTTGACAA GCACTTTGGGCCTTTCTTTCGCACGGAGCAGCTTATCATCCAGGCCCCCAACACCAGTGTTCATATCTAC GAACCGTACCCCGCAGGAGCCGATGTGCCCTTCGGGCCTCCATTGAACAAAGAGATTCTGCACCAGGTTC TGGACTTACAGATCGCCATTGAAAGCATCACCGCATCTTACAACAATGAAACCGTGACACTGCAGGACAT CTGTGTGGCCCCCCTCTCTCCATACAACAAGAACTGCACCATTATGAGTGTGTTAAATTACTTCCAGAAC AGCCATGCGGTGCTGGACAGCCAAGTAGGCGACGACTTCTATATCTACGCTGATTACCACACACACTTTC TGTACTGTGTACGGGCTCCCGCCTCCTTGAATGATACGAGTTTGCTCCACGGTCCTTGCCTGGGTACATT TGGAGGACCGGTGTTCCCGTGGCTTGTGTTGGGTGGCTATGATGATCAGAACTACAACAATGCCACCGCG CTTGTGATCACCTTCCCCGTGAATAATTACTACAATGACACAGAGAGGCTCCAGAGGGCCTGGGCCTGGG AGAAAGAGTTTATTAGTTTTGTGAAAAACTACAAGAATCCAAATCTGACCATTTCTTTCACTGCTGAGCG AAGCATCGAAGATGAGCTCAATCGGGAAAGTAACAGTGACGTGTTCACCGTCATCATCAGCTACGTCGTG ATGTTTCTGTACATTTCCCTCGCCCTGGGTCACATCCAGAGCTGCAGCAGGCTCCTGGTGGATTCTAAGA TCTCGCTGGGCATTGCGGGGATCCTGATCGTGCTAAGCTCGGTGGCCTGCTCTCTGGGCATCTTCAGCTA CATGGGGATGCCGCTGACCCTCATCGTCATTGAGGTCATCCCATTCCTGGTGCTGGCTGTCGGGGTGGAC AACATCTTCATTCTAGTGCAGACCTACCAGAGAGATGAGCGTCTTCAGGAGGAAACGCTGGATCAGCAGC TGGGCAGGATCCTTGGAGAAGTGGCCCCGACCATGTTCCTTTCATCCTTTTCTGAGACCTCAGCATTTTT CTTTGGGGCGCTGTCCTCGATGCCAGCTGTGCACACCTTCTCTCTGTTTGCGGGAATGGCCGTCCTCATT GACTTCCTCCTTCAGATTACCTGCTTTGTGAGCCTGTTGGGGTTAGATATTAAGAGGCAAGAGAAAAACC ATCTGGACATCCTGTGCTGTGTCAGAGGCGCTGACGACGGACAAGGTAGCCACGCCTCCGAAAGCTACCT GTTTCGCTTCTTCAAAAACTACTTTGCCCCCCTTCTGCTGAAGGACTGGCTGAGGCCAATTGTGGTAGCG GTGTTTGTGGGCGTTCTGTCATTCAGTGTTGCGGTGGTGAACAAAGTAGACATCGGGTTGGATCAGTCTC TCTCAATGCCAAACGATTCGTATGTGATTGACTATTTCAAATCACTCGCTCAGTACCTGCACTCGGGCCC ACCCGTGTACTTTGTCCTGGAGGAAGGCTATAACTACAGTTCACGCAAAGGGCAGAACATGGTGTGCGGC GGCATGGGCTGTGACAATGACTCCCTGGTGCAGCAGATATTTAACGCAGCTGAGCTGGACACCTACACCC GAGTAGGCTTCGCCCCCTCGTCCTGGATCGATGACTACTTTGACTGGGTCTCGCCACAGTCGTCCTGCTG CAGACTCTACAACGTCACTCACCAGTTCTGCAATGCTTCTGTGATGGACCCAACCTGTGTCCGCTGCAGA CCTCTGACTCCAGAGGGTAAACAGAGGCCTCAGGGGAAAGAATTCATGAAATTCCTGCCCATGTTCCTTT CTGATAACCCCAACCCCAAGTGCGGCAAAGGGGGACATGCTGCTTACGGTTCAGCTGTTAACATTGTGGG AGATGACACTTACATTGGGGCCACTTACTTCATGACCTACCACACCATACTTAAGACCTCCGCTGACTAT ACTGATGCCATGAAGAAAGCTCGGCTAATAGCCAGTAACATCACGGAAACCATGCGTTCTAAGGGGAGTG ACTACCGCGTATTCCCTTACAGTGTGTTCTACGTCTTCTATGAACAGTACCTGACCATTATTGATGACAC CATCTTTAACCTCAGTGTGTCTCTGGGCTCCATATTTCTGGTGACCTTGGTGGTTCTGGGCTGTGAGCTG TGGTCTGCGGTCATCATGTGTATCACCATAGCCATGATCCTGGTCAACATGTTCGGTGTCATGTGGCTGT GGGGCATCAGTCTGAATGCGGTCTCCTTGGTCAACTTGGTGATGAGCTGCGGCATTTCTGTGGAGTTCTG CAGCCATATAACGAGAGCATTCACCATGAGTACCAAAGGAAGCCGAGTGAGCCGGGCGGAAGAGGCACTG GCCCACATGGGTAGTTCTGTATTCAGTGGAATCACACTTACGAAATTTGGAGGGATCGTGGTGTTAGCCT TTGCCAAATCTCAAATTTTTGAGATATTTTACTTCAGGATGTACTTAGCCATGGTCTTACTCGGAGCCAC TCATGGACTAATATTTCTTCCCGTCTTACTCAGTTACATAGGGCCGTCGGTGAATAAAGCTAAAAGACAC ACCACTTACGAGCGCTACAGAGGGACAGAGAGAGAACGGCTCCTCAATTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008720 |
| Insert Size | 3834 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008720.2, NP_032746.2 |
| RefSeq Size | 5209 bp |
| RefSeq ORF | 3834 bp |
| Locus ID | 18145 |
| UniProt ID | O35604 |
| Gene Summary | Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment (PubMed:21896731, PubMed:22048958, PubMed:27551080). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket (By similarity). May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211885 | Npc1 (tGFP-tagged) - Mouse Niemann Pick type C1 (Npc1) |
CNY 13,970.00 |
|
| MR211885 | Npc1 (Myc-DDK-tagged) - Mouse Niemann Pick type C1 (Npc1) |
CNY 13,336.00 |
|
| MR211885L3 | Lenti ORF clone of Npc1 (Myc-DDK-tagged) - Mouse Niemann Pick type C1 (Npc1) |
CNY 14,630.00 |
|
| MR211885L4 | Lenti ORF clone of Npc1 (mGFP-tagged) - Mouse Niemann Pick type C1 (Npc1) |
CNY 15,736.00 |
