Diaph1 (NM_007858) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D18Wsu154; D18Wsu154e; Di; Dia1; Diap1; Drf; Drf1; mDi; p140m; p140mDia
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224083 representing NM_007858 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTCCGGCGGGGGCCTGGGGCCCGGCCGCGGTACCCGGGACAAGAAGAAGGGTCGGAGCCCGG ATGAGCTGCCTGCGACGGGCGGCGACGGCGGCAAACATAAGAAATTTCTGGAGAGATTTACCAGCATGAG GATTAAGAAGGAGAAAGAAAAGCCCAATTCTGCTCATAGAAACTCCTCTGCATCGTACGGAGATGACCCC ACTGCTCAGTCATTGCAGGACATCTCAGACGAGCAAGTTCTTGTCCTCTTTGAGCAGATGCTGGTGGATA TGAACCTGAATGAGGAGAAGCAGCAGCCTTTGCGAGAGAAGGACATTGTCATCAAGAGGGAGATGGTGTC GCAATATCTGCACACTTCCAAGGCTGGCATGAACCAGAAAGAGAGCTCTAGGTCTGCCATGATGTACATC CAGGAGCTGAGGTCGGGCTTGCGGGATATGCACCTGCTTAGCTGCCTTGAGTCCCTTCGAGTCTCTCTCA ACAATAACCCTGTCAGTTGGGTGCAGACATTTGGTGCTGAGGGCCTAGCCTCCTTATTGGACATCCTCAA ACGACTCCATGATGAGAAAGAGGAGACTTCTGGAAACTACGACAGCCGAAACCAGCATGAGATTATCCGC TGTTTGAAGGCTTTCATGAACAACAAGTTTGGAATCAAAACTATGTTGGAGACGGAAGAAGGAATCCTAC TGCTGGTCAGAGCCATGGATCCTGCTGTTCCCAATATGATGATTGATGCAGCAAAGCTGCTGTCTGCCCT CTGTATCCTGCCGCAGCCGGAGGACATGAATGAACGAGTTCTAGAGGCAATGACAGAGAGAGCTGAAATG GATGAGGTCGAACGCTTCCAGCCACTTCTGGACGGATTAAAAAGTGGGACCTCTATTGCCCTCAAAGTGG GATGCCTACAGCTCATCAATGCTCTCATCACTCCAGCTGAGGAACTGGACTTCCGAGTTCACATCCGAAG TGAGCTGATGCGCCTGGGGCTGCATCAGGTGTTGCAGGAGCTTCGAGAGATTGAAAATGAAGATATGAAA GTACAGCTGTGCGTGTTTGATGAACAAGGGGATGAAGATTTCTTTGATCTGAAGGGACGGCTGGATGATA TCCGCATGGAGATGGATGACTTTGGTGAAGTTTTTCAGATTATTTTAAACACAGTGAAAGATTCAAAGGC AGAGCCACACTTCCTGTCTATCTTGCAGCATCTCCTGTTGGTCCGAAATGATTATGAAGCCAGGCCACAG TACTATAAACTGATTGAAGAATGTGTTTCTCAGATAGTTCTACACAAAAATGGAACTGATCCTGACTTCA AGTGCCGACACCTGCAGATTGATATTGAGAGATTGGTTGATCAAATGATTGATAAAACAAAGGTGGAAAA ATCTGAGGCCAAAGCTACAGAGCTGGAAAAAAAGTTGGATTCAGAATTAACAGCGCGGCACGAGTTACAA GTAGAAATGAAAAAGATGGAAAATGACTTTGAGCAGAAACTTCAGGATCTTCAAGGAGAAAAGGATGCCT TGGATTCTGAAAAGCAGCAGATCACTGCACAGAAACAAGACCTGGAGGCAGAGGTGTCCAAGCTGACAGG AGAGGTTGCCAAGCTGTCAAAAGAACTAGAAGATGCCAAGAATGAAATGGCTTCTCTCTCTGCTGTGGTT GTTGCACCTTCTGTTTCTAGCAGTGCTGCTGTTCCCCCTGCCCCTCCTCTGCCTGGTGACTCTGGCACTG TTATTCCACCTCCCCCACCCCCACCTCCTCTTCCTGGAGGTGTGGTCCCACCATCCCCTCCTCTGCCTCC AGGTACTTGTATCCCTCCACCTCCTCCTTTACCTGGAGGTGCTTGTATACCCCCTCCCCCCCAGTTGCCT GGCAGTGCTGCCATCCCTCCACCTCCTCCTCTACCTGGAGTTGCTTCCATCCCCCCACCTCCCCCTTTGC CTGGGGCTACTGCCATCCCCCCACCTCCCCCTTTGCCTGGGGCTACTGCCATCCCCCCACCTCCCCCTTT GCCTGGAGGTACAGGTATACCACCACCACCTCCTCCTTTGCCTGGAAGTGTTGGCGTTCCCCCACCCCCT CCCTTGCCTGGAGGACCAGGACTGCCTCCTCCCCCTCCCCCTTTTCCTGGAGCACCTGGCATTCCTCCAC CTCCACCTGGTATGGGCGTGCCTCCACCTCCCCCCTTTGGATTTGGGGTTCCTGCGGCCCCAGTTCTGCC ATTTGGATTAACCCCCAAAAAAGTTTATAAGCCAGAGGTGCAGCTCCGGAGGCCAAACTGGTCCAAGTTT GTGGCTGAGGACCTTTCCCAGGACTGCTTCTGGACAAAGGTGAAGGAGGACCGCTTTGAGAACAATGAAC TTTTTGCCAAACTTACCCTTGCCTTCTCCGCCCAGACCAAGACTTCTAAAGCCAAGAAGGATCAAGAAGG TGGAGAAGAAAAGAAATCTGTTCAAAAGAAGAAAGTAAAAGAGCTGAAAGTGCTGGATTCAAAGACAGCG CAGAATCTCTCAATCTTTTTGGGTTCATTCCGCATGCCCTATCAAGAGATAAAGAACGTTATCCTGGAGG TGAATGAGGCTGTTCTCACAGAGTCTATGATCCAGAACCTCATTAAACAGATGCCAGAGCCAGAGCAGCT AAAGATGCTCTCTGAACTGAAGGAGGAGTACGATGATCTGGCTGAGTCAGAGCAGTTTGGTGTGGTGATG GGCACAGTGCCCCGCCTTCGGCCTCGCCTCAACGCCATCCTCTTCAAGCTACAGTTCAGTGAGCAAGTTG AGAACATCAAGCCAGAGATCGTGTCTGTCACCGCCGCATGCGAAGAGCTGCGTAAGAGTGAGAACTTCTC CAGCCTCCTGGAGCTCACACTGCTGGTCGGAAACTATATGAATGCGGGCTCCAGGAATGCTGGTGCTTTC GGCTTCAATATCAGCTTCCTTTGTAAGCTTCGAGACACCAAGTCTGCAGATCAGAAGATGACTCTGTTGC ATTTCTTGGCTGAGTTATGTGAGAATGACCACCCCGAAGTCCTCAAGTTTCCTGATGAGCTTGCCCATGT AGAGAAAGCCAGCAGAGTCTCTGCTGAGAACCTGCAGAAGAGCTTAGATCAGATGAAGAAGCAGATTGCG GACGTGGAGCGCGATGTTCAGAATTTCCCAGCTGCCACTGACGAGAAGGACAAGTTTGTTGAGAAGATGA CCAGCTTTGTGAAGGATGCACAGGAACAGTATAACAAACTACGGATGATGCACTCCAACATGGAGACCCT CTATAAGGAGCTAGGTGACTACTTCGTCTTTGACCCTAAGAAGTTGTCTGTAGAGGAATTCTTTATGGAT CTGCACAACTTTAGGAATATGTTTTTGCAAGCAGTCAAGGAAAACCAGAAGCGCCGGGAAACAGAAGAAA AGATGCGGAGAGCAAAATTAGCCAAGGAGAAGGCAGAAAAAGAGCGACTGGAGAAGCAGCAGAAGCGCGA GCAGCTCATCGACATGAACGCAGAGGGGGATGAGACAGGTGTGATGGACAGTCTTCTAGAAGCTCTGCAG TCAGGGGCAGCATTCCGACGGAAGAGAGGGCCCCGGCAGGTCAACAGGAAGGCTGGGTGTGCAGTCACAT CTCTGCTAGCCTCGGAGCTGACCAAGGATGATGCCATGGCTCCTGGTCCTGTTAAGGTACCCAAGAAAAG TGAAGGAGTCCCCACAATCCTGGAAGAAGCCAAGGAGCTGGTTGGCCGTGCAAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007858
Insert Size	3768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007858.3, NP_031884.1
RefSeq Size	5633 bp
RefSeq ORF	3768 bp
Locus ID	13367
UniProt ID	O08808
Gene Summary	This gene encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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