Apaf1 (NM_001042558) Mouse Untagged Clone

CAT#: MC224055

Apaf1 (untagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, (10ug)



  "NM_001042558" in other vectors (4)

CNY 16,060.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224055 representing NM_001042558
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT
ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC
CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA
TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG
TGTCGTCTTCCAGTGGCAAGGACACAGATGGTGGAATAACTTCATTTGTAAGGACAGTGCTGTGTGAAGG
TGGAGTACCCCAGAGGCCGGTTATTTTCGTTACTAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTC
TGGAAACTGAATGGAGAACCAGGGTGGGTCACCATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAG
CTGCGGAAGCCGTTCGAGATCACTCCCTCTTAGAAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCAT
TGGAAAACAAGACAAATCTGGGCTTCTCATGAAACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAG
AGTTTCTCTCAGAGGCTTCCACTTAATATTGAGGAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCA
AACACCCAAGGTCTCTGTTGATCTTGGATGATGTTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCA
GTGTCAGATTCTTCTTACAACCAGAGATAAGAGTGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTC
CCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGACTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAG
AAGATCTGCCAGCGGAGGCTCACAGTATTATAAAGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAAT
TGGTGCGCTTTTACGTGATTTTCCCAATCGCTGGGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTT
AAGAGAATAAGGAAGTCTTCATCTTATGATTATGAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAA
TGCTCAGAGAAGACATCAAAGACTATTACACAGACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTAC
AAAGGTGTTGTGCGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAAT
AAGTCTCTCTTATTCTGTAATCGGAATGGAAAGTCATTTTGTTATTATTTACATGATCTTCAAGTAGATT
TTCTTACAGAGAAGAATCGCAGTCAGCTTCAGGATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTA
TTACCAGCCCCACACGCTGTCTCCAGACCAGGAGGACTGCATGTATTGGTACAACTTCCTAGCCTATCAC
ATGGCTAGTGCCAATATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAA
CAGAACTTGTCGGCCCTGCCCATCTGATTCACGAGTTCGTGGCATATAGGCATATATTGGATGAAAAGGA
TTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTTATCTTTAAATGGACACCTCCTTGGACGACAGCCATTT
CCTAATATTGTACAGCTGGGCCTCTGTGAACCAGAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGG
CCAAGCAGGAGGGGGATACTGGGCGCCTTTACCTGGAATGGATAAACAAAAAAACTATCAAGAATCTGTC
CCGCTTAGTCGTCCGCCCCCACACAGATGCTGTTTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATA
GCTTCTTGTGGGGCTGATAAAACCTTACAGGTGTTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCA
AAGCTCACGAAGATGAGGTGCTTTGCTGCGCGTTCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGC
GGATAAGAAGGTTAAGATTTGGGATTCTGCGACTGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAG
CAAGTCAATTGCTGCCATTTCACCAACAAGAGTAACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCT
TCCTCAAGCTCTGGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCACACGAACTCAGTCAA
CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGG
GATGTGAGATCAGCAAACGAGAGGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTC
CAGAGGATGTGGAGGTGATCGTGAAGTGTTGTTCCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGC
AAAAAACAAAGTCCTCCTTTTTGATATTCATACTAGTGGCCTATTGGCAGAGATCCACACAGGCCATCAC
AGCACCATCCAGTACTGTGACTTCTCCCCCTATGACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTG
TGGAGTTGTGGAACATAGACTCCCGCCTAAAGGTGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGG
TGTGATGTTTTCTCCCGATGGCTCCTCATTTTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG
ACAAAAAAGGTATGCAAGAACTCTGCCATCGTGCTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACG
AAACGATGGTCCTTGCAGTTGACAACATAAGAGGCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGA
TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGAT
GAAGATGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGA
AAGCTGTGCGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGAT
TCAGGTATGGAATTGGCAGACAGGGGACTATGTATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTC
AGGCTCCTCCAAGATTCAAGATTGCTTTCTTGGTCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTA
CCGGAAGAATAGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC
GACCAAGTTTTCATCTACCTCTGCTGATAAGACTGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTT
CATGAGCTGAAGGGCCATAATGGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAA
CTGGAGATGACAATGGAGAAATCCGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCC
GATCTCGGTAGAGGAAGGAACTGCTACCCACGGCGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGT
AAAACGCTTGTCTCTGCTGGAGGATATCTCAAGTGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCT
TCTACACAAATGGAACAAACCTCAAGAAAATCCACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGA
TAATCTCGGTATTTTATATATTTTACAGGTTTTAGAGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001042558
Insert Size 3750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001042558.1, NP_001036023.1
RefSeq Size 6557 bp
RefSeq ORF 3750 bp
Locus ID 11783
UniProt ID O88879
Gene Summary Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) has an additional segment in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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