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Apaf1 (NM_001042558) Mouse Untagged Clone
CAT＃: MC224055
Apaf1 (untagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, (10ug)
CNY 16,060.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224055 representing NM_001042558
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG TGTCGTCTTCCAGTGGCAAGGACACAGATGGTGGAATAACTTCATTTGTAAGGACAGTGCTGTGTGAAGG TGGAGTACCCCAGAGGCCGGTTATTTTCGTTACTAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTC TGGAAACTGAATGGAGAACCAGGGTGGGTCACCATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAG CTGCGGAAGCCGTTCGAGATCACTCCCTCTTAGAAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCAT TGGAAAACAAGACAAATCTGGGCTTCTCATGAAACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAG AGTTTCTCTCAGAGGCTTCCACTTAATATTGAGGAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCA AACACCCAAGGTCTCTGTTGATCTTGGATGATGTTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCA GTGTCAGATTCTTCTTACAACCAGAGATAAGAGTGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTC CCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGACTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAG AAGATCTGCCAGCGGAGGCTCACAGTATTATAAAGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAAT TGGTGCGCTTTTACGTGATTTTCCCAATCGCTGGGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTT AAGAGAATAAGGAAGTCTTCATCTTATGATTATGAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAA TGCTCAGAGAAGACATCAAAGACTATTACACAGACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTAC AAAGGTGTTGTGCGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAAT AAGTCTCTCTTATTCTGTAATCGGAATGGAAAGTCATTTTGTTATTATTTACATGATCTTCAAGTAGATT TTCTTACAGAGAAGAATCGCAGTCAGCTTCAGGATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTA TTACCAGCCCCACACGCTGTCTCCAGACCAGGAGGACTGCATGTATTGGTACAACTTCCTAGCCTATCAC ATGGCTAGTGCCAATATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAA CAGAACTTGTCGGCCCTGCCCATCTGATTCACGAGTTCGTGGCATATAGGCATATATTGGATGAAAAGGA TTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTTATCTTTAAATGGACACCTCCTTGGACGACAGCCATTT CCTAATATTGTACAGCTGGGCCTCTGTGAACCAGAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGG CCAAGCAGGAGGGGGATACTGGGCGCCTTTACCTGGAATGGATAAACAAAAAAACTATCAAGAATCTGTC CCGCTTAGTCGTCCGCCCCCACACAGATGCTGTTTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATA GCTTCTTGTGGGGCTGATAAAACCTTACAGGTGTTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCA AAGCTCACGAAGATGAGGTGCTTTGCTGCGCGTTCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGC GGATAAGAAGGTTAAGATTTGGGATTCTGCGACTGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAG CAAGTCAATTGCTGCCATTTCACCAACAAGAGTAACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCT TCCTCAAGCTCTGGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCACACGAACTCAGTCAA CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGG GATGTGAGATCAGCAAACGAGAGGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTC CAGAGGATGTGGAGGTGATCGTGAAGTGTTGTTCCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGC AAAAAACAAAGTCCTCCTTTTTGATATTCATACTAGTGGCCTATTGGCAGAGATCCACACAGGCCATCAC AGCACCATCCAGTACTGTGACTTCTCCCCCTATGACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTG TGGAGTTGTGGAACATAGACTCCCGCCTAAAGGTGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGG TGTGATGTTTTCTCCCGATGGCTCCTCATTTTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG ACAAAAAAGGTATGCAAGAACTCTGCCATCGTGCTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACG AAACGATGGTCCTTGCAGTTGACAACATAAGAGGCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGA TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGAT GAAGATGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGA AAGCTGTGCGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGAT TCAGGTATGGAATTGGCAGACAGGGGACTATGTATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTC AGGCTCCTCCAAGATTCAAGATTGCTTTCTTGGTCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTA CCGGAAGAATAGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC GACCAAGTTTTCATCTACCTCTGCTGATAAGACTGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTT CATGAGCTGAAGGGCCATAATGGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAA CTGGAGATGACAATGGAGAAATCCGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCC GATCTCGGTAGAGGAAGGAACTGCTACCCACGGCGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGT AAAACGCTTGTCTCTGCTGGAGGATATCTCAAGTGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCT TCTACACAAATGGAACAAACCTCAAGAAAATCCACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGA TAATCTCGGTATTTTATATATTTTACAGGTTTTAGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001042558 |
| Insert Size | 3750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001042558.1, NP_001036023.1 |
| RefSeq Size | 6557 bp |
| RefSeq ORF | 3750 bp |
| Locus ID | 11783 |
| UniProt ID | O88879 |
| Gene Summary | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) has an additional segment in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226529 | Apaf1 (tGFP-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1) transcript variant 1, (10ug) |
CNY 10,550.00 |
|
| MR226529 | Apaf1 (Myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 8,696.00 |
|
| MR226529L3 | Lenti ORF clone of Apaf1 (Myc-DDK-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 11,500.00 |
|
| MR226529L4 | Lenti ORF clone of Apaf1 (mGFP-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1 |
CNY 11,500.00 |
