Cilp (NM_173385) Mouse Untagged Clone

CAT#: MC224053

Cilp (untagged) - Mouse cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (Cilp), (10ug)



  "NM_173385" in other vectors (3)

CNY 16,060.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9830114D16; C130036G17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224053 representing NM_173385
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAATCAAGACTTGGGTATTCTCTTTCCTGGTCCTTGAGGTCACCACCGTGCTGGGGAGACAGA
CGATGCTTGCCCAGTCAGTGAGAAGAGTCCAGCCTGTGAAGAGGACCCCCAAGACCCTTGCCAAGCCTGC
CGACTCCCAGGAGAGTCCCGGAGAGTGGACAACATGGTTTAACATCGACCACCCAGGTGGGCAGGGTGAC
TATGAGCGGCTGGATGCCATTCGCTTCTACTATGGGGAGCGTGTGTGTGCCCGTCCCCTGCGGCTAGAGG
CCCGGACCACTGACTGGATGCCTGCGGGCAGCACTGGCCAGGTGGTCCACGGCAGTCCCCGTGAGGGCTT
CTGGTGCCTCAACAGGGAGCAGAGACCAGGCCAGAACTGTTCCAATTATACCGTGCGCTTCCTCTGCCCA
CCAGGGTCCTTGCGAGGAGATGCAGAGCACATCTGGAGTTCCTGGTCTCCCTGGAGCAAGTGCTCAGCTG
CCTGCGGTCACACTGGGGTGCAGACCCGTACCCGCACCTGCTTGGCACAGACAGTGTCACTGTGCAGTGA
GGCGACCGAGGAGGGCCAGCTCTGCATGAGCCAGGCCTGCACAGCTTGTGACCTGACCTGCCCCATGGGC
CAGGTAAATGCTGACTGTGATGCCTGCATGTGCCAGGACTTCATGCTTCACGGGGCCATCTCCCTCCCTG
GAGGTGGCCCAGCTCCAGGAGCCGCTGTCTACCTGCTGGCCAAGGCACCAAAGATGCTGACCCGAACAGA
CAGCAGCGGGAGGTTCCGAGTTCCTGGCTTGTGTCCTGATGGCAAAACCATCCTGAAAATCACCAAGACC
AAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTGAAGTCAGCTACCATCAATGCGGAGTTTG
TGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGATGAAAGCACGTCGGGCTGGGCAGAGTGTGTC
TCTGTGCTGCAAAGCCACGGGGAAACCCAGTCCGGACAAGTACTTCTGGTACCACAACAACACACTGCTG
GATCCCTCCCTCTATAAGCACGAGAGCAAGCTGGTGCTGAGGAACCTGCAGCAGGACCAAGCAGGGGAGT
ACTTCTGTAAGGCTCAGAGTGATGCTGGGGCTGTGAAGTCCAAGGTCACCCAGCTCACTGTCATTGCACA
TGATGAGACTCCTTGCAACCCAACCCCAGAGAGCTACCTTATCCGGCTGCCCCATGACTGCTTTCAGAAC
GCCAGCAATTCCTTCTACTACGACGTAGGCCGCTGCCCGATCAAGACCTGTGCAGGGCAGCAGGACAATG
GGATCCGGTGCCGAGATGCTGTGGAGAACTGCTGCGGCATTTCCAGAACAGAAGAGAGGGAGATCCAGTG
CAGTGGGTACACACTGCCCACCAAGGTGGCTGTGGAGTGCAGCTGCCAACGATGCGCAGAGACACGGAGC
ATCGTCCGGGGCCGAGTCACTGCTGCTGACAACGGGGAGCCCATGCGCTTTGGCCATGTGTACATGGGGA
ATAACCGCGTGAGCATGACTGGCTACAAAGGCACCTTCACACTCCACATCCCCCAGGACACGGAGAGGCT
GGTGCTCACATTTGTGGACAGGCTGCAGAAGTTTGTCAACACAACCAAAGTGCTGCCCTTCAACAAGAAG
GGAAGCGCAGTGTTCCACGAGATCAAGATGCTTCGGCAGAAAGAGCCCATCACGTTGGAAGCCATGGAGA
CCAACATTATCCCCCTGGGAGAGGTGATTGGTGAAGACCCTGTGGCTGAGCTGGAGATCCCATCCAAAAG
TTTCTATAGGCAGAACGGGGAGCCCTTCACAGGAAAGGTAAAGGCCAGCGTGACCTTCCTGGATCCTCGG
AACATCTCGACGGCCACAGCTGCCCAAAGTGACCTCAACTTCATCAATGATGAAGGAGACACTTTTCCCC
TTAGAACATATGGTATGTTTTCAGTGGACTTTAGAGATGAGGCCACTTCCGAGTCACTTAATGCTGGCAA
GGTGAAGGTCCACCTTGACTCGACCCAGGTCAAAATGCCAGAGCATGTGCCAGCCATGAAACTCTGGTCG
CTCAACCCAGACACAGGACTGTGGGAAGAGGAAGGGGATTTCAAGTTTGAAAGCCAAAGGAGGAACAAGA
GGGAAGAGAGAACCTTCCTAGTGGGCAATATGGAGATCCGGGAGAGAAGGCTCTTTAACCTGGACGTCCC
TGAAAGCCGAAGGTGCTTCATTAAGGTGAGGACTTACCGGAGTGAGAGGTTCCTGCCCAGTGAGCAAATT
CAGGGAGTTGTGGTCTCAGTGATCAATCTGGAGCCCAGAACTGGCTTCTCATCTAACCCCAGGGCCTGGG
GCCGATTTGACAGTGTCATCACAGGGCCCAATGGGGCCTGCCTGCCTGCCTTCTGTGATGATCAGTCCCC
CGATGCTTACTCTGTCTATGTCTTGGCAAGCCTTTCTGGAGAAGAACTAGAGGCAGTTGAGTCTTCTCCT
AAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTCAACAAACTTAAATACCGTCGGACAGACC
ATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTATGGCCAAACCAAGGCCCAACTCAGCTGA
GGAGAGCAATGGGCCTATCTATGCCTTTGAGAACCTTCGGGCGTGTGAGGAGGCACCCCCCAGTGCAGCT
CACTTCCGGTTCTATCAGATCGAGGGGGATCGCTATGACTATAACACAGTTCCTTTCAATGAAGATGACC
CTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGCCAATGGAGTTCAGGGCCTGCTATATCAA
GGTTAAGATTGTGGGGCCACTGGAGGTGAATGTACGATCCCGTAACATGGGAGGCACCCACCGGCAGACT
GTGGGGAAGCTCTATGGGATCCGGGATGTGAAGAGTACTCGGGACAGAGACCAACCTAATGTCTCATCTG
CCTGCCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAGACCGTGTAGACCGCACATTAGTAAAGGT
TATCCCCCAGGGCAGCTGCCATCGAGCTAGTGTTAACTCTATGTTGCACGAGTACCTGGTCAACCACCTT
CCCCTGGCAGTCAACAATGACACCAGCGAGTATACTATGCTGGCACCCCTGGACCCTCTGGGCCACAATT
ACGGTATCTATACTGTTACTGATCAGGACCCTCGTACAGCTAAGGAGATTGCGCTCGGCCGGTGCTTTGA
TGGCACTTCCGATGGCTCCTCCAGAATCATGAAGAGCAATGTGGGAGTTGCCCTGACCTTTAACTGTGCA
GAAAGGCAGGTAGGCCGCCAGAGTGCCTTCCAGTACCTCCAAAGCACACCGGCCAGGTCCCCAGCTACAG
GCACTGTCCAAGGAAGAGTACCAGCCATGAGGCAACAACGGGCAAGCAGGGGTGGCCTACGCCGGCGTGG
AAGCATGGGCCCCTCTGAGATTTTCTGGTGTTGCTCAACAACCTCTGAGCAACTAAGTCTTGTGGTTCTT
AGTCCTTTCTGTCCATCTCATGTGACAGCTGTCGTGAGACTGATGCACAGACTGTCACTTGTTAATTTAA
ACATATCTGGTTTGGTGCAGTTTGTTTCTTTGTGCCTTTACTTACTGTCTCTGCCTCATGATGCTGATTG
CCACAGGGCCCCCACGATGACAAAACAAAGATTCTCCTCTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_173385
Insert Size 3753 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173385.2, NP_775561.1
RefSeq Size 4153 bp
RefSeq ORF 3753 bp
Locus ID 214425
UniProt ID Q66K08
Gene Summary Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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