Polg (NM_017462) Mouse Untagged Clone

CAT#: MC223968

Polg (untagged) - Mouse polymerase (DNA directed), gamma (Polg), (10ug)



  "NM_017462" in other vectors (4)

CNY 12,720.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA409516; PolgA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223968 representing NM_017462
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGCCTGCTCTGGAAGAAGGTGGCTGGCGCCAAGGTCGCCTCAGGGCCAGTACCAGCAACCGCGC
GCTGGGTCTCCAGCTCCGTCCTCGACCCCGTCCCCAGCGACGGGCGGCCGCCGTCGCAAATGCCCTCCTC
CGAGAATGGGCAGCTGCGGCTCAACCCTCTGCTCATCCAGATGTTGTCGAGAGGCCTGCACGAGCAGATC
TTCGGGTGCGGCGGGGAAATGCCCGACGAGGCCGCGGTGCAGCGCAGCGTAGAGCACCTGCAGAAGCACG
GGCTCTGGGGGCAGCCGGCCACGCCCTTGCCAGACGTGGAGCTGCGCCTGCCCCGGCTCTTCGGGGGCAA
CCTGGACCAGCACTTCCGCCTCCTGGCCCAGAAGCAGAGCCTGCCTTACTTGGAGGCGGCCGCCTCGTTA
TTGGAGGCCCAATTGCCCCCCGAGCCCAAGAGCTGGGCCTGGGCGGAGGGCTGGACCCGGTACGGCCCCG
AGGGGGAGGCCGAACCCGTGGCCATCCCCGAGGAGCGGGCCCTGGTGTTCGACGTGGAGGTCTGCTTGGC
AGAGGGAACCTGCCCCACTTTGGCGGTGGCCATATCCCCCTCGGCCTGGTATTCCTGGTGCAGCCGGCGG
CTGGTGGAAGAGCGTTACTCTTGGACCAGCCAGCTATCGCCGGCTGACCTAATCCCTTTGGGGGGCTCCA
CTAGTGCCAGCAGCTCCACCAAGCAGGATGGGCAGGAACAGTTAGTGGTGGGGCACAATGTTTCCTTTGA
CCGAGCCCATATCAGGGAACAGTATCTGATTCAGGACTCCCGGATGCGTTTTCTCGATACTATGAGCATG
CACATGGCCATCTCGGGGCTGAGCAGCTTCCAGCGCAGCCTGTGGATGGGAGCCAAGCAGGGAAAGCACA
AGACCCAGCAGTCCACAAAGCGAGGGCAGAAGTCCCCGAGGAAAGCCAATGGTCCAGCGATCTCATCTTG
GGACTGGATGGATATCAGCAGTGCCAATAATCTTGCAGATGTGCACAACCTTTATGTGGGGGGACCTCCC
TTAGAGAAGGAGCCTCGGGAGCTGTTCGTCAAAGGCAGCATGAGGGATATCCGAGAGAACTTCCAGGATC
TGATGCAGTACTGCGCCCGTGATGTGTGGGCCACCTTTGAGGTTTTCCAGCAGCAGCTGCCACTCTTCTT
GGAGAGGTGTCCCCACCCAGTGACTCTGGCTGGCATGCTGGAGATGGGTGTGTCCTACCTGCCTGTCAAC
CAGAACTGGGAGCGTTACCTGACAGAGGCACAGAACACATATGAGGAGCTCCAGCGGGAGATGAAGAAGT
CGCTGATGGATCTGGCTAATGATGCCTGTCAGTTGCTCTCAGGAGAGAGGTACAAAGAAGACCCTTGGCT
CTGGGACCTAGAATGGGATTTGCAGGAGTTTAAGCAGAAAAAGGCAAAGAAGGTGAAGAAGCCAGCCTCA
GCCAGCAAGTTGCCCATCGAGGGAGCTGGGCCCTTTGGGGATCCCATGGATCAGGAAGATCCTGGCCCGC
CCAGCGAGGAGGAGGAGCTTCAGCGAAGTGTAACAGCCCACAACCGGTTACAGCAGCTGAGGAGCACCAC
GGACCTCCTGCCTAAGCGACCCCAGCACCTTCCAGGACACCCTGGGTGGTACCGGAAGCTCTGCCCTCGA
CTAGATGACCCTGCCTGGGCTCCCGGCCCCAGCCTCCTCAGCCTGCAAATGCGGGTCACTCCTAAGCTCA
TGGCACTGACCTGGGATGGTTTTCCTCTACACTACTCAGACTCTCATGGTTGGGGCTACCTAGTGCCTGG
GCGTCGGGATAATCTGACCGAGCCGCCAGTGAGCCCCACTGTAGAATCCGCTGCAGTAACCTGCCCCTAC
AGAGCCATCGAGTCCTTATACAGGAAGCACTGCCTTGAACAGGGGAAGCAGCAATTGGAGCCCCAGGAGG
TAGACCTGGCTGAGGAGTTCTTACTCACTGACAGTAGTGCCATGTGGCAAACGGTAGAAGAACTGGGCTG
CTTAGACGTAGAGGCTGAGGCCAAGATGGAGAATTCAGGGCTGAGCCAACCTCTAGTTCTGCCTGCTGCT
TGTGCCCCCAAATCCAGCCAGCCCACGTATCACCATGGCAACGGACCTTATAATGATGTGAATATCCCTG
GTTGCTGGTTTTTCAAGCTGCCTCACAAGGACGGTAACAACTACAATGTGGGCAGTCCCTTTGCCAAAGA
TTTCCTGCCCAAGATGGAGGATGGCACCCTGCAGGCTGGCCCAGGAGGTGCCAGTGGGCCTCGTGCCCTG
GAAATAAATAAAATGATTTCTTTTTGGAGGAATGCTCATAAACGTATCAGTTCCCAGATGGTGGTATGGC
TCCCCAGGTCAGCTCTGCCCCGGGTTGTGACCAGGCACCCCAGCTTCGATGAGGAAGGCCACTATGGGGC
CATCCTACCCCAGGTGGTGACTGCTGGCACCATCACCCGTCGAGCTGTGGAGCCCACGTGGCTCACTGCC
AGTAATGCTCGGCCTGACCGGGTAGGCAGTGAGTTGAAAGCCATGGTGCAGGCTCCACCTGGCTATGTCC
TTGTGGGTGCTGATGTGGACTCACAGGAACTGTGGATCGCAGCTGTACTTGGAGATGCTCACTTTGCTGG
GATGCATGGCTGCACGGCCTTTGGCTGGATGACTCTGCAGGGCAGGAAGAGCAGAGGCACTGATCTGCAC
AGCAAGACAGCTGCCACTGTGGGCATCAGCCGAGAGCATGCCAAAATCTTCAACTATGGCCGCATCTATG
GGGCTGGGCAGTCCTTTGCTGAGCGCCTACTGATGCAGTTCAACCACAGGCTCACAAGGCAGGAGGCAGC
TGAGAAGGCCCAGCAGATGTATGCAGTCACAAAAGGCCTGCGCCGGTATCGGCTGTCTGCGGATGGTGAG
TGGCTGGTGAAACAGTTGAATCTTCCTGTGGACAGGACAGAGGACGGCTGGGTCTCCCTACAAGATCTTC
GAATGATCCGAAGAGAAGCTTCAAGGAAGTCACGATGGAAGAAGTGGGAGGTAGCCTCTGAACGAGCATG
GACAGGGGGCACAGAGTCAGAAATGTTTAATAAGCTGGAGAGTATTGCCATGTCTGATACACCACGTACC
CCAGTACTGGGCTGCTGCATCAGCAGAGCCTTGGAGCCCTCAGTTGTCCAGGGAGAGTTTATAACCAGTC
GTGTGAACTGGGTGGTACAGAGCTCTGCTGTAGACTACTTACATCTCATGCTTGTGGCCATGAAGTGGCT
GTTTGAGGAATTTGCCATTGATGGGCGCTTCTGCATCAGCATCCACGACGAGGTTCGCTACCTGGTGCGT
GAGGAGGACCGCTACCGTGCCGCCCTGGCACTGCAGATCACCAATCTCCTGACCAGGTGCATGTTTGCCT
ATAAGCTGGGTCTGAATGATCTGCCCCAGTCAGTCGCCTTTTTCAGTGCAGTAGACATTGACCAGTGCCT
CAGGAAGGAAGTGACCATGGACTGTAAAACTCCTTCTAACCCAACTGGGATGGAAAGGAGATATGGGATC
CCCCAGGGTGAAGCACTGGATATTTACCAGATAATTGAACTCACCAAAGGCTCCTTGGAAAAACGAAGCC
AGCCTGGACCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_017462
Insert Size 3654 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017462.2, NP_059490.2
RefSeq Size 4596 bp
RefSeq ORF 3654 bp
Locus ID 18975
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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