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Grm5 (NM_001081414) Mouse Untagged Clone
CAT＃: MC223835
Grm5 (untagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant a, (10ug)
CNY 12,240.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6430542K11Rik; AI850523; Glu5R; Gprc1e; mGluR5; mGluR5b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223835 representing NM_001081414
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTTCTGTTGATTCTGTCAGTCCTACTTCTGAAAGAAGATGTACGAGGAAGTGCACAGTCCAGTG AGAGGAGGGTGGTGGCTCACATGCCAGGTGACATTATTATTGGAGCTCTCTTCTCGGTCCACCACCAACC AACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCAGTCCGTGAGCAGTATGGCATTCAGAGAGTGGAA GCCATGCTGCATACCTTGGAAAGGATCAATTCAGATCCCACACTCTTGCCCAACATCACACTAGGCTGTG AGATAAGAGATTCCTGCTGGCATTCTGCTGTGGCCCTAGAGCAAAGCATTGAGTTTATAAGGGATTCCCT CATCTCTTCGGAAGAGGAAGAAGGCTTGGTACGCTGTGTAGATGGCTCTTCTTCCTTCCGCTCCAAGAAA CCCATAGTGGGAGTCATTGGGCCTGGCTCGAGTTCTGTGGCCATTCAAGTTCAGAACTTGCTCCAGCTTT TCAACATACCTCAGATTGCTTACTCTGCAACTAGCATGGATTTGAGTGACAAGACTCTATTCAAGTACTT CATGAGGGTTGTACCTTCGGATGCCCAGCAAGCCCGAGCCATGGTAGACATAGTGAAGAGATACAACTGG ACTTATGTCTCAGCTGTGCACACAGAAGGCAACTATGGAGAAAGTGGGATGGAGGCTTTCAAAGATATGT CAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAAATCTACAGCAATGCTGGGGAACAGAGCTTTGA CAAGCTGTTGAAAAAGCTCAGAAGTCATTTACCTAAAGCCCGGGTGGTAGCCTGCTTCTGTGAAGGCATG ACAGTTCGAGGTCTGCTCATGGCCATGAGACGCTTGGGTCTAGCAGGGGAATTTCTACTTCTGGGCAGTG ATGGCTGGGCTGACAGGTATGACGTGACAGATGGGTATCAGCGAGAAGCTGTCGGTGGGATTACAATCAA GCTCCAGTCTCCTGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCAGAAACAAACCTCAGA AACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGGCTAGAAGGGTTTGCACAGGAGAACA GCAAGTACAACAAGACTTGCAACAGTTCTCTAACTCTGAGAACGCATCATGTTCAAGATTCCAAAATGGG ATTTGTGATCAATGCAATCTATTCTATGGCTTATGGGCTCCACAACATGCAGATGTCCCTGTGTCCAGGC TATGCAGGCCTCTGTGATGCAATGAAGCCAATTGATGGGCGGAAACTTTTGGACTCCCTGATGAAAACCA ACTTTACTGGAGTTTCCGGAGATATGATTCTATTTGATGAAAATGGAGACTCTCCAGGAAGGTATGAAAT AATGAATTTCAAGGAAATGGGAAAAGATTATTTTGATTACATCAATGTTGGAAGTTGGGACAATGGGGAA TTAAAAATGGATGATGATGAAGTGTGGTCCAAGAAAAATAACATCATCAGATCTGTGTGCAGTGAACCGT GTGAGAAAGGACAGATAAAGGTGATCCGGAAGGGAGAAGTCAGCTGTTGTTGGACCTGCACACCTTGTAA GGAGAATGAGTATGTTTTTGATGAGTACACCTGCAAGGCGTGCCAACTGGGGTCCTGGCCCACTGACGAC TTGACAGGTTGTGATTTGATCCCAGTCCAGTATCTTCGATGGGGTGACCCTGAGCCCATTGCAGCTGTGG TGTTTGCCTGCCTCGGTCTGCTAGCCACCCTCTTCGTTACTGTAATCTTCATCATTTATCGGGACACTCC AGTGGTCAAGTCTTCCAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGCCTGGGTTACTTGTGT ACCTTCTGCCTCATTGCAAAGCCCAAACAGATTTACTGCTACCTTCAGAGAATTGGCATCGGTCTCTCTC CAGCCATGAGCTACTCAGCCCTTGTAACGAAGACCAACCGTATTGCAAGGATTCTAGCTGGCAGCAAGAA GAAGATCTGTACCAAAAAGCCCAGATTCATGAGCGCCTGTGCTCAGTTAGTGATCGCTTTCATTCTCATC TGTATACAGTTGGGTATTATTGTGGCCCTCTTTATCATGGAGCCTCCGGATATAATGCATGACTATCCAA GCATCCGAGAAGTCTACTTGATTTGTAACACCACCAACCTAGGGGTTGTCACTCCTCTTGGATACAATGG ATTATTGATTTTGAGTTGCACATTCTATGCGTTCAAGACCAGAAATGTTCCAGCCAACTTTAACGAGGCC AAATATATTGCTTTCACCATGTACACAACCTGCATCATATGGCTGGCCTTTGTGCCTATCTACTTTGGCA GCAACTACAAAATCATCACCATGTGTTTCTCAGTCAGCCTCAGTGCCACAGTGGCCCTGGGTTGCATGTT TGTGCCGAAGGTGTACATCATCCTAGCCAAACCGGAGAGAAATGTGCGCAGCGCCTTCACAACCTCTACA GTGGTGCGCATGCACGTAGGAGATGGCAAGTCATCATCCGCTGCCAGCAGATCCAGCAGCCTAGTCAACC TGTGGAAGAGGAGGGGCTCGTCTGGGGAAACCCTAAGCTCCAATGGAAAATCCGTGACTTGGGCCCAGAA TGAGAAGAGCACCCGGGGGCAGCACCTGTGGCAGCGACTGTCTGTCCATATCAACAAGAAGGAGAACCCC AACCAGACAGCAGTCATCAAACCCTTCCCCAAGAGCACAGAGAGCCGCGGGCAGGGTGCAGGGGCAGGTG GTGGCTCTGGCCCCGGTGCAGCTGGTGCTGGTAGCGCAGGATGCACAGCGACAGGCGGCCCAGAGCCACC AGACGCCGGCCCCAAGGCGCTTTATGATGTCGCAGAGGCAGAGGAGCGCTTCCCAGCGGCTGCCAGGCCG CGCTCGCCATCGCCCATCAGTACGCTGAGCCACCTGGCAGGCTCGGCGGGCCGCACAGACGACGACGCGC CGTCGCTGCACTCGGAGACCGCTGCACGCAGCAGCTCATCCCAGGGCTCGCTCATGGAGCAGATTAGCAG CGTGGTGACGCGCTTCACCGCCAACATCACCGAGCTCAACTCCATGATGTTGTCCACCGCGGCTGCGCCG GGGCCCCCTGGTACCCCTATCTGCTCTTCCTACCTGATCCCCAAAGAGATCCAGCTGCCCACGACCATGA CGACCTTCGCAGAGATCCAGCCTCTGCCGGCCATCGAGGTGACCGGAGGAGCTCAGCCGGCGACAGGGCC ATCACCTGCCCAAGAGACGCCCGCAGGAGCTGAAGCCGCCCCAGGAAAACCGGATCTGGAGGAGCTGGTG GCCCTCACTCCACCATCGCCCTTCAGGGACTCGGTGGACTCGGGGAGCACCACCCCAAACTCTCCAGTCT CCGAATCGGCCCTCTGCATCCCATCCTCTCCCAAATATGACACTCTCATCATCAGAGATTACACGCAGAG TTCTTCATCGTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001081414 |
| Insert Size | 3516 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081414.2, NP_001074883.1 |
| RefSeq Size | 8428 bp |
| RefSeq ORF | 3516 bp |
| Locus ID | 108071 |
| Gene Summary | G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (a) lacks an alternate in-frame exon in the 3' coding region, compared to variant b. The resulting isoform (a) lacks a segment near the C-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227397 | Grm5 (tGFP-tagged) - Mouse glutamate receptor metabotropic 5 (Grm5) transcript variant a, (10ug) |
CNY 12,830.00 |
|
| MR227397 | Grm5 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant a |
CNY 11,690.00 |
|
| MR227397L3 | Lenti ORF clone of Grm5 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant a |
CNY 13,590.00 |
|
| MR227397L4 | Lenti ORF clone of Grm5 (mGFP-tagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant a |
CNY 13,590.00 |
