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Washc5 (NM_153548) Mouse Untagged Clone
CAT＃: MC223759
E430025E21Rik (untagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik), (10ug)
CNY 14,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AL022848; C76463; E430025E21Rik; Kiaa0196; mKIAA0196; strumpellin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223759 representing NM_153548
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGACTTCCTGGCTGAGAATAATCTCTGTGGCCAGGCCATCCTGAGGATCGTCTCCTGTGGCAATG CCATCATTGCCGAGGTGCTGCGGCTCTCTGAGTTCATTCCTGCTGTGTTCCTGCTGAAAGACCGGGCTGA CCAGCAGAGATATGGAGACATCATCTTTGACTTCAGCTACTTTAAGGGTCCAGAGTTCTGGGAAAGCAAA CTGGAAGCCAAGCCTGAGCTGCAGGATTTAGATGAAGAATTTCGGGAAAACAACATAGAAATCGTGACCA GATTTTATTTAGCCTTTCAAAGTGTGCATAAATACATTGTAGACTTAAACAGATACCTCGATGACCTCAA TGAAGGAGTTTATATTCAGCAAACCTTAGAAACCGTGCTTCTCAGTGAAGATGGAAAACAACTTCTATGT GAAGCCCTGTACTTATATGGAGTTATGCTGCTTGTCATCGACCAGAAGATTGAAGGAGAAGTCAGAGAGA GAATGCTGGTTTCTTACTATCGATACAGTGCTGCTCGGTCCTCTGCTGACTCAAACATGGATGATATCTG TAAGCTGCTACGAAGCACAGGATATTCCAGCCAGCCAGGAGCCAAAAGACCACCCAACTATCCTGAGAGC TATTTCCAGAGAGTGCCTATCAACGAGACGTTCATCAGCATGGTCATCGGACGCCTGAGGTCTGATGACA TTTACAACCAGGTCTCTGCCTACCCGCTGCCGGAGCACCGCAGCACTGCCCTGGCCAATCAAGCCGCCAT GCTCTACGTGATTCTCTATTTTGAACCTTCCATTCTTCACACCCACCAAGCAAAAATGAGAGAGATCGTG GATAAGTACTTTCCAGATAACTGGGTAATCAGCATCTATATGGGTATCACTGTTAATCTAGCTGATGCCT GGGAACCTTACAAAGCTGCGAAAACTGCTCTCAACAACACCCTTGACCTCGCCAATGTCAAAGAACAGGC AAGCAGATACGCTAGTGTCAGCGACAGAGTGCGCGCTCAGGTGCAGCAGTTTCTGAAAGAGGGCTACCTG AGGGAGGAGGTGCTTCTGGACAACATCCCCAGGCTGCTGAACTGCCTGAGAGACTGCAATGTCGCCATCC GCTGGCTAATGCTCCACACAGCCGACTCAGCCTGTGACCCAAACAACAAGCGCCTTCGTCAAATCAAGGA CCAGATTCTGGCAGACTCTAGGTACAATCCCAAGATCCTCTTCCAGTTGCTATTGGACACTGCACAATTC GAGTTTATACTCAAAGAGATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGA AGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGGTGAAACCACTAACCCGAGTGGA GAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGAT TCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGT TGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAAC CATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTA ATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAG CCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGA TCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATT CCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTA CACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCAC TCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTG GATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACC GGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCAC CATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGG CAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAGA TTCAAGACTGGCAGAGTATGTACCAATCCACTCATATACCAATCCCGAAGTTTGCACCTGTGGATGAGTC TATAACATTTATTGGTCGACTCTGCAGAGAAATCTTGCGGATCACAGACCCAAAAATGACATGTTACATA GACCAGCTGAACACTTGGTATGACGTGAAAACTCATCAAGAAGTGACCAGCAGTCGCCTCTTCTCAGAAA TCCAGACCACCTTGGGGACTTTTGGTCTGAATGGATTGGACAGGCTTCTGTGCTTCATGATTGTGAAAGA GCTACAGAATTTCCTCAGTATGTTTCAGAAAATTATCCTGAAAGAGAGAACTGTTCAAGAAACTTTAAAG ATGCTCATGAGTGCTGTTAACCCTCTAAAGAGCATAGTGGCAAATTCAAGTAAAGTTTATCTTTCTGCCA TCACCAAAACACAGAAGATCTGGAGTGCTTACCTGGAGGCTATAATGAAGGTTGGACAGATGCAGATCCT GAGACAACAGATCGCCAATGAGCTAAACTCTTCCTGTCGATTTGATTCCAGACATCTGGCAGCTGCCCTG GATAATCTCAATAAGGCTCTCCTGGCAGACATCGAAGCCCACTACCGGGACCCTTCGCTTCCTTACCCCA AAGAAGATAACACACTCTTATATGAAATCACAGCCTATCTGGAGGCGGCTGGCATTCACAACCCCCTGAA TAAGATTTACATAACAACGAAGCGCTTACCCTATTTCCCGATTGTCAACTTTCTGTTTTTGATCGCGCAG TTGCCAAAACTTCAGTATAACAAAAATCTAGGCATGGTCTGCCGGAAGCCTGCCGACCCTGTTGACTGGC CACCGCTCGTCCTAGGTCTGCTCACGCTGCTGAAGCAGTTCCACTCCCGGTACACCGAGCAATTCCTGGC ACTGATTGGCCAGTTTATTCGCTCCACAATGGAGCAGTGCACAAGCCAGAAGATGCCTGAAATGCCTGCA GATGCTGTGGGCGCCCTTCTCTTCCTGGAGGATTATGTTCGGTATACAAAACTCCCCCGAAGGGTGGCTG AAGCCCATGTGCCTAACTTCATTTTTGATGAATTCCGAACAGTCCTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_153548 |
| Insert Size | 3480 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153548.2, NP_705776.2 |
| RefSeq Size | 4038 bp |
| RefSeq ORF | 3480 bp |
| Locus ID | 223593 |
| UniProt ID | Q8C2E7 |
| Gene Summary | Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (By similarity). Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation (PubMed:24998208).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211724 | E430025E21Rik (tGFP-tagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik) |
CNY 9,220.00 |
|
| MR211724 | E430025E21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik) |
CNY 8,880.00 |
|
| MR211724L3 | Lenti ORF clone of E430025E21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik) |
CNY 10,260.00 |
|
| MR211724L4 | Lenti ORF clone of E430025E21Rik (mGFP-tagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik) |
CNY 10,260.00 |
