Magi3 (NM_133853) Mouse Untagged Clone

CAT#: MC223656

Magi3 (untagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 2, (10ug)



  "NM_133853" in other vectors (4)

CNY 14,350.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4732496O19Rik; 6530407C02Rik; AA407180; AI120132; mKIAA1634
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223656 representing NM_133853
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAAGACGTTGAAGAAGAAGAAGCACTGGCTCAGCAAGGTGCAGGAGTGTGCGGTATCCTGGGCCG
GGCCCCCGGGCGACTTGGGCGCCGAGATCCGCGGCGGCGCCGAGCGCGGCGAGTTCCCTTACCTGGGGCG
GCTCCGCGACGAGGCTGGCGGCGGCGGAGGCACCTGCTGCGTGGTCTCGGGCAAGGCGCCCAGTCCGGGC
GATGTGCTGTTGGAGGTGAACGGGACGCCGGTCAGTGGGCTCACCAACCGGGACACTCTGGCTGTCATCC
GCCACTTCCGCGAGCCCATCCGTCTGAAGACGGTGAAGCCAGGCAAAGTTATAAATAAAGATTTGCGACA
CTACCTGAGTCTTCAGTTTCAGAAAGGATCAATTGACCACAAACTACAGCAAGTCATCAGAGATAATCTC
TATTTGAGAACCATCCCATGCACTACAAGGGCTCCCAGGGATGGGGAAGTCCCAGGGGTGGATTATAACT
TCATTTCTGTTGAACAATTCAAGGCACTGGAAGAAAGCGGAGCATTGTTAGAAAGTGGAACGTATGATGG
AAATTTCTATGGCACACCCAAGCCTCCAGCAGAGCCTAGTCCTTTCCAGCCCGATCCAGTTGATCAGGTC
CTCTTTGACAATGAGTTTGACACAGAATCCCAAAGAAAAAGAACCACATCTGTCAGCAAGATGGAAAGGA
TGGACAGCTCTCTCCCTGAAGAAGAAGAAGATGAGGACAAAGAAGCTGTTAATGGCAGCGGAAGCATGGA
AACTAGAGAGATGCATTCTGAGACATCTGACTGCTGGATGAAGACTGTTCCAAGTTATAACCAAACAAAT
AGCTCCATGGACTTTAGAAATTATATGATGAGAGATGAGAATCTGGAACCGCTGCCCAAAAACTGGGAAA
TGGCCTACACTGACACAGGAATGATCTACTTCATTGATCATAATACGAAGACAACCACCTGGTTGGATCC
TCGTCTCTGCAAGAAAGCCAAAGCCCCTGAAGACTGTGAAGATGGAGAGCTTCCCTATGGCTGGGAGAAA
ATAGAGGACCCTCAGTATGGAACATACTACGTTGATCACCTCAACCAGAAAACTCAGTTTGAAAATCCAG
TGGAGGAAGCCAAGAGGAAAAAGCAGTTAGGACAGGCTGAAATTCATTCTGCAAAAACAGATGTGGAAAG
AGCACACTTTACTCGGGACCCATCCCAACTTAAAGGTGTACTTGTTCGAGCATCGCTGAAAAAAAGCACC
ATGGGCTTTGGCTTTACCATTATTGGTGGAGATAGGCCTGATGAATTTCTACAAGTAAAAAACGTGCTCA
AAGATGGTCCTGCCGCTCAGGATGGGAAAATTGCACCAGGTGATGTTATTGTAGACATCAATGGCAACTG
TGTCCTTGGTCACACCCATGCAGATGTTGTCCAGATGTTTCAACTGGTACCTGTCAACCAGTATGTCAAC
CTTACTTTATGCCGTGGTTATCCACTTCCTGATGACAGTGAAGATCCTGTTGTGGACATTGTTGCTGCTA
CCCCTGTCATCAATGGACAATCTTTAACAAAGGGAGAGACATGCATGAATACTCAGGATTTTAAACTGGG
AGCAATGGTTTTGGATCAGAATGGAAAATCAGGACAAATCTTGGCCAGTGATCGTCTCAATGGTCCATCT
GAGTCAAGTGAGCAGAGGGCATCTCTGGCATCATCAGGCAGCTCACAGCCTGAACTAGTGACTATCCCTC
TGATTAAGGGCCCCAAAGGCTTTGGGTTTGCAATTGCTGACAGCCCAACTGGACAGAAGGTGAAAATGAT
ATTGGATAGCCAGTGGTGTCAAGGCCTTCAAAAAGGGGATATCATCAAAGAAATTTACCATCAGAATGTG
CAGAATTTAACACATCTCCAAGTCGTAGAAGTCCTAAAGCAGTTCCCAGTAGGTGCAGATGTTCCATTGC
TTATCTTAAGAGGAGGTCCTTGTTCACCAACTAAAACTGCCAAAACGAAAACAGATACAAAGGAAAATTC
AGGAAGTTTGGAGACTATAAATGAGCCTATTCCCCAGCCTATGCCTTTTCCGCCCAGCATAATCAGATCA
GGATCCCCAAAATTGGATCCTTCTGAGGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCAA
ACACCAAAGATCTGGATGTCTTTCTTCGGAAACAAGAATCAGGGTTTGGCTTCAGGGTGCTAGGAGGAGA
TGGACCTGACCAGTCTATATATATTGGGGCCATCATTCCCCTGGGAGCAGCTGAAAAAGATGGTAGGCTC
CGTGCTGCTGATGAATTGATGTGTATTGATGGGATTCCTGTTAAAGGAAAATCACACAAGCAAGTATTAG
ACTTAATGACTACTGCTGCTCGAAATGGACACGTGCTACTAACTGTCAGGAGGAAGATCTTCTATGGAGA
GAAACAACCCGAGGACGAAAGCCATCAAGCCTTCTCACAGAACGGATCTCCTCGCCTGAATCGGGCAGAG
CTTCCAACCAGGTCTGCCCCACAGGAGGCCTATGATGTCACCTTACAGAGGAAAGAAAATGAAGGGTTTG
GTTTTGTCATCCTCACCTCTAAAAGCAAGCCGCCCCCAGGAGTTATTCCTCATAAAATTGGCCGGGTCAT
AGACGGAAGTCCAGCTGATCGTTGTGGAGGACTGAAAGTTGGAGATCACATCTCTGCTGTGAATGGGCAG
TCCATTGTCGACCTATCCCATGATAATATTGTTCAGCTCATCAAAGATGCTGGAGTCACCGTCACGCTGA
CAGTGGTTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACT
ACAGCACAGGCCCATGGGACAAGCACAAGCCAACCACATACCTGGGGACAGAATTGCCCTAGAAGGTGAA
ATTGGGAGAGATGTCTGCAGTTCTTACAGACATTCCTGGTCTGACCATAAGCACCTTGCACAGCCTGACA
CTGCAGTGATTTCAGTTGTGGGCAGTCGGCACAATCAGAGCCTTGGCTGTTACCCTGTGGAGCTGGAGAG
AGGCCCTCGAGGCTTTGGGTTCAGCCTCCGCGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTGCGC
CTGGCCGAGGACGGGCCCGCCATCAAAGACGGCAGGATTCACGTTGGTGACCAGATCGTTGAAATCAATG
GGGAACCGACACAAGGCATCACACACACTCGAGCAATTGAGCTCATTCAGGCTGGTGGGAATAAAGTCCT
CCTCCTTCTGAGGCCAGGAACTGGCTTGATACCTGACCACGGTTTGGCTCCTTCCGGTCTGTGCTCCTAC
GTGAAACCTGAGCAACATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133853
Insert Size 3381 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133853.3, NP_598614.1
RefSeq Size 6672 bp
RefSeq ORF 3381 bp
Locus ID 99470
UniProt ID Q9EQJ9
Gene Summary Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.[UniProtKB/Swiss-Prot Function]
Transcript Variant: this variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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