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Trim33 (NM_001079830) Mouse Untagged Clone
CAT＃: MC223655
Trim33 (untagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 2, (10ug)
CNY 14,350.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 8030451N04Rik; AI413936; Ecto; Tif1g |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223655 representing NM_001079830
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAAACAAAGGCGGCGGTGAGGCAGAGAGTGGCGGCGGGGGCAGCGGTAGCGCGCCGGTAACCG CCGGGGCCGCCGGGCCCACGGCGCAGGAGGCGGAGCCGCCTCTCGCCGCGGTGCTGGTGGAGGAGGAGGA GGAGGAAGGCGGCAGGGCTGGCGCGGAGGGCGGTGCGGCTGGGCCCGACGACGGGGGGGTGGCCGCGGCC TCCTCAAGCTCGGCCCCGGCCGCCTCGGTCCCCGCGGCCTCAGTGGGCTCCGCGGTCCCCGGAGGCGCGG CATCGACGCCGGCTCCAGCTGCTGCCCCAGCCCCGGCCCCGGCCCCGGCCCCGGCCCCGGCCCCCGCTCC CGCCCCGGCTCCTGGTTCCTCGTCGGGGCCACCTCTCGGACCACCAGCCTCGCTTCTGGACACCTGCGCG GTGTGTCAGCAGAGCTTGCAGAGCCGGCGCGAGGCGGAGCCCAAGCTGCTCCCCTGCCTGCACTCCTTCT GCCTGCGCTGTCTGCCGGAGCCGGAGCGCCAGCTCAGCGTGCCCATCCCTGGGGGCAGCAACGGTGACGT CCAGCAAGTTGGTGTAATACGGTGTCCAGTATGTCGTCAAGAATGTAGACAGATAGATCTTGTGGATAAT TATTTTGTGAAAGACACATCTGAAGCTCCTAGCAGCTCTGATGAGAAATCAGAACAGGTATGTACTAGTT GTGAAGATAATGCAAGTGCAGTTGGTTTTTGTGTAGAATGTGGAGAGTGGCTGTGTAAGACATGCATTGA AGCACATCAAAGAGTAAAATTCACTAAAGACCACCTGATCAGGAAAAAAGAAGATGTCTCAGAGTCTGTT GGAACATCTGGTCAGCGCCCAGTGTTTTGTCCTGTACATAAACAAGAGCAGTTGAAACTATTCTGTGAAA CGTGTGATAGATTGACGTGTAGAGACTGTCAACTTTTGGAGCACAAAGAACATAGGTATCAGTTTTTAGA AGAGGCTTTTCAAAATCAGAAAGGTGCAATTGAAAATCTGTTGGCTAAGCTTCTTGAAAAGAAGAATTAT GTTCATTTTGCAGCTACACAAGTGCAGAATAGGATTAAAGAAGTAAATGAGACTAACAAACGTGTAGAAC AGGAAATTAAAGTGGCCATCTTCACCCTTATCAATGAAATTAATAAGAAAGGAAAATCTCTCTTGCAACA GCTAGAGAATGTTACAAAAGAAAGACAGATGAAGTTACTACAGCAGCAGAATGATATCACAGGCCTCTCC CGGCAGGTGAAGCATGTTATGAACTTTACAAACTGGGCCATTGCAAGTGGCAGCAGCACGGCCCTACTTT ATAGCAAGCGTCTGATTACTTTTCAGTTACGTCATATTTTGAAAGCCCGATGTGATCCTGTTCCTGCTGC CAATGGAGCAATTCGTTTCCATTGTGATCCCACCTTCTGGGCCAAAAATGTAGTGAATTTAGGTAATCTA GTAATAGAGAGTAAGCCAGCTCCTGGTTATACTCCTAATGTTGTAGTTGGGCAAGTTCCTCCAGGGACAA ACCACATTAGTAAAACCCCTGGACAGATTAATTTAGCACAGCTTCGACTCCAGCACATGCAGCAACAAGT GTATGCACAGAAACATCAGCAGTTACAACAGATGAGACTGCAGCAACCACCAGCTCCTATACCAACAACA ACAGCAACAACCCAGCAGCATCCTAGGCAAGCAGCCCCACAGATGTTACAACAACAGCCTCCAAGATTGA TCAGTGTGCAAACAATGCAAAGAGGCAACATGAACTGTGGAGCATTTCAAGCCCATCAAATGAGACTGGC TCAGAATGCTGCCAGAATACCAGGGATCCCCAGGCACAGTGCCCCTCAGTATTCCATGATGCAGCCACAT CTCCAAAGACAACATTCAAATCCAGGACATGCTGGACCGTTTCCTGTTGTATCAGCACACAACCCAATCA ACCCAACAAGTCCCACCACAGCAACTATGGCAAATGCAAATCGAGGTCCCACCAGCCCGTCTGTTACAGC AATAGAATTAATACCCTCAGTCACCAATCCAGAAAACCTTCCATCGTTGCCAGATATCCCACCTATACAG TTGGAAGATGCTGGCTCAAGTAGTTTAGATAATCTACTGAGTAGATATATCTCAGGCAGTCATCTACCCC CTCAGCCTACAAGTACCATGAATCCTTCCCCAGGACCCTCTGCGTTATCACCTGGATCTTCAGGCTTATC CAATTCTCACACACCTGTGAGACCCCCTAGCACGTCTAGTACTGGCAGTCGAGGCAGCTGCGGATCATCA GGAAGAACTGCTGAGAAAAGTGCTCATAGTTTCAAGTCTGATCAGGTGAAGGTCAAGCAGGAACCTGGGA CTGAAGAAGAGATATGCAGCTTTTCAGGAGCTGTGAAACAAGAGAAGACAGAGGATGGCAGGAGGAGTGC CTGTATGTTGAGCAGTCCTGAGAGTAGTTTGACACCACCTCTTTCAACTAACCTTCACCTAGAGAGTGAG CTGGATACATTAACAGGCCTGGAGAACCACGTGAAGACTGAACCTACAGATATCAGTGAAAGCTGCAAAC AGTCAGGACTCAGTAACCTAGTCAATGGAAAGTCTCCAATTCGAAACCTCATGCACAGGTCGGCAAGGAT TGGAGGGGATGGCAATAGCAAAGATGATGACCCAAATGAAGACTGGTGTGCCGTCTGCCAGAACGGAGGG GATCTTTTGTGTTGTGAGAAATGTCCGAAGGTCTTTCATCTTACCTGTCATGTTCCAACACTTCTCAGCT TTCCAAGTGGAGACTGGATATGCACATTTTGCAGAGATATTGGGAAGCCAGAAGTTGAATATGATTGTGA TAATATGCAGCATAGTAAGAAGGGGAAAACTGCACAAGGCTTAAGCCCTGTGGACCAAAGGAAATGTGAA CGTCTTCTGCTTTACCTCTATTGTCACGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGTTTCGATAC CAAACTACTATAAAATTATAAAGAAACCGATGGATTTATCAACGGTGAAGAAGAAGCTTCAGAAAAAACA TTCCCAGCATTACCAAATCCCAGATGACTTTGTGGCTGATGTCCGTTTGATCTTCAAGAACTGTGAAAGG TTTAATGAAGGTGATTCAGAAGTAGCTAAGGCAGGGAAAGCAGTTGCATTGTACTTTGAAGATAAACTCT CAGAGATCTACTCAGACAGGACCTTCACTCCTTTGCCAGAGTTTGAGCAGGACGAAGATGATGGTGAAGT AACAGAAGACTCTGACGAAGATTTTATACAGCCTCGTAGAAAACGCCTAAAGTCAGACGAGAGACCAGTA CATATAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001079830 |
| Insert Size | 3372 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001079830.2, NP_001073299.1 |
| RefSeq Size | 8819 bp |
| RefSeq ORF | 3372 bp |
| Locus ID | 94093 |
| UniProt ID | Q99PP7 |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227458 | Trim33 (tGFP-tagged) - Mouse tripartite motif-containing 33 (Trim33) transcript variant 2, (10ug) |
CNY 12,350.00 |
|
| MR227458 | Trim33 (Myc-DDK-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 2 |
CNY 11,210.00 |
|
| MR227458L3 | Lenti ORF clone of Trim33 (Myc-DDK-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 2 |
CNY 13,110.00 |
|
| MR227458L4 | Lenti ORF clone of Trim33 (mGFP-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 2 |
CNY 13,110.00 |
