Phkb (NM_199446) Mouse Untagged Clone

CAT#: MC223468

Phkb (untagged) - Mouse phosphorylase kinase beta (Phkb), (10ug)



  "NM_199446" in other vectors (4)

CNY 13,870.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI463271
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223468 representing NM_199446
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACTCACCTGATGCAGCCTTCTCTTCACCTGCTTTGTTAAGGTCTGGCTCAGTTTATGAACCTC
TTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGATAAGTTGGATCATTATTACAGAAT
TGTCAAGTCAACGATGCTGATGTATCAAAGTCCAACTACCGGTCTCTTTCCTACTAAAACATGTGGTGGA
GAAGAGAAGTCCAAAGTGCACGAGAGTCTGTACTGTGCTGCGGGTGCCTGGGCCTTGGCGCTAGCATACA
GGCGCATTGATGATGACAAGGGACGGACCCATGAGCTGGAGCACTCGGCCATAAAGTGTATGAGAGGAAT
TCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAACAGTTTAAACAAGATCCCCGGCCCACAACATGT
CTTCACTCTGTTTTCAGTGTGCACACTGGGGATGAGTTGCTCAGCTATGAAGAATATGGCCATCTTCAGA
TAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTAGAAATGATTTCCTCAGGCCTCCAGATAATCTATAA
CACGGATGAGGTTTCTTTTATTCAAAACCTTGTATTTTGTGTAGAGAGAGTTTACCGTGTTCCTGACTTT
GGTGTCTGGGAAAGAGGAAGCAAATATAACAATGGTAGTACCGAACTGCACTCAAGCTCTGTTGGTTTAG
CAAAAGCAGCCTTAGAAGCAATTAATGGATTTAACCTCTTTGGAAACCAGGGGTGTTCCTGGTCAGTTAT
ATTTGTGGATCTCGATGCTCACAACCGCAACAGGCAGACATTGTGCTCACTGTTACCCAGAGAATCACGC
TCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCAGTTACCCTGCGTTTGCCTTGGATGATGAAGCAC
TGTTCAGCCAGACACTTGATAAAGTGATAAGAAAATTGAAAGGAAAATATGGATTTAAGCGTTTCTTGCG
AGATGGGTACAGAACGCCATTGGAAGATCCCAACAGACGCTATTACAAACCAGCAGAGATTAAGCTGTTT
GATGGCATTGAATGTGAATTTCCCATATTCTTCCTTTACATGATGATCGATGGTGTTTTTAGAGGCAATC
TTGAACAAGTCAAGGAATATCAGGACCTTTTGACACCTTTGCTTCATCAGACCACAGAAGGATATCCTGT
TGTGCCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATGTGAAAAAAGAAACCCTGGTAGTCAGAAG
CGGTTTCCAAGTAACTGTGGCCGTGATGGGAAGTTGTTTCTTTGGGGACAAGCTCTTTATATCATTGCAA
AACTCCTAGCTGATGAGTTAATCAGCCCTAAAGACATTGACCCTGTCCAGCGCTTTGTCCCACTTCAGAA
TCAACGTAACGTGAGCATGAGATACTCCAATCAGGGCCCACTGGAAAATGACTTGGTGGTTCATGTAGCA
CTTGTAGCAGAAAGCCAACGCCTTCAAGTTTTTCTCAATACATATGGTATTCAAACCCAAACTCCTCAGC
AGGTGGAACCCATTCAGATCTGGCCTCAGCAGGAGCTTGTAAAAGCTTATTTCCACCTGGGAATCAATGA
AAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTTGGTACATCAAAGATTTATCGCATT
CTAGGAAAGACTGTGGTTTGTTACCCTATCATCTTTGACCTGAGTGATTTCTACATGTCTCAGGACGTTC
TGCTGCTCATAGATGACATAAAGAACGCACTACAGTTCATTAAGCAATATTGGAAAATGCATGGACGCCC
ACTTTTTCTTGTTCTCATCCGAGAAGACAACATAAGAGGTAGCAGATTCAACCCAATATTAGACATGCTG
GCAGCCTTTAAAAAGGGAATAATTGGAGGAGTCAAAGTTCATGTTGATCGTTTACAGACATTGATATCCG
GAGCTGTAGTAGAGCAGCTTGACTTTCTACGAATTAGTGACACAGAAAAGCTTCCAGAATTTAAGAGCTT
TGAAGAACTAGAATTTCCTAAGCATTCAAAAGTCAAACGGCAGAGCAGTACCGCAGATGCTCCTGAGGCG
CAACACGAGCCAGGCATCACCATTACCGAGTGGAAAAACAAATCCACCCACGAAATCCTTCAGAAGCTGA
ATGACTGCGGTTGTCTGGCTGGTCAAACCATCCTGCTGGGTATACTGCTCAAAAGAGAGGGCCCTAACTT
TATCACAATGGAAGGAACTGTGTCTGATCACATTGAGAGAGTGTATAGAAGAGCTGGCAGCAAAAAGCTT
TGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGGACAGCCTGGCCCCATCCATTACTA
ATGTTTTAGTGCAGGGCAAACAGGTAACTCTAGGTGCCTTTGGACATGAAGAGGAGGTCATCTCTAACCC
ACTGTCTCCAAGAGTGATTAAGAACATAATCTATTATAAGTGCAATACCCACGATGAGAGGGAGGCAGTC
ATTCAGCAAGAACTGGTCATCCACATCGGCTGGATCATCTCCAACAGCCCTGAGTTGTTCAGCGGCATGC
TGAAAATACGCATTGGGTGGATCATCCACGCCATGGAGTATGAACTGCAGGTCCGGGGCGGGGATAAGCC
AGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAAGTGAAGCAGCTGCTGCTAGACATTCTCCAGCCTCAA
CAGAGTGGAAGATGTTGGTTGAATAGACGTCAGATTGATGGGTCATTGAATAGAACACCCCCGGAATTCT
ATGACCGAGTGTGGCAGATCCTGGAACGCACACCTAATGGTATCGTTGTAGCTGGAAAGCATTTGCCACA
GCAACCAACTTTGTCGGATATGACCATGTATGAGATGAATTTTTCTCTCCTTGTTGAAGATATGCTGGGA
AACATTGACCAGCCAAAGTATAGACAGATCATAGTTGAGTTACTAATGGTCGTATCCATTGTGCTGGAAA
GAAACCCTGAACTGGAGTTTCAAGACAAAGTGGATCTGGACAGACTGGTTAAAGAAGCGTTTCATGAGTT
TCAAAAAGATGAGAGTCGGCTAAAAGAAATCGAAAAACAAGATGATATGACTTCCTTTTACAACACGCCC
CCACTGGGAAAAAGAGGAACCTGCAGCTACTTGACGAAGGTTGTGATGAACTCACTGCTCGAGGGAGAAG
TCAAGCCCAGCAATGAGGACTCCTGCCTGGTTAGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_199446
Insert Size 3258 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_199446.1, NP_955517.1
RefSeq Size 4640 bp
RefSeq ORF 3258 bp
Locus ID 102093
UniProt ID Q7TSH2
Gene Summary Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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