Dna2 (NM_177372) Mouse Untagged Clone

CAT#: MC223434

Dna2 (untagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2), (10ug)



  "NM_177372" in other vectors (4)

CNY 13,680.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Dna2l; E130315B21Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223434 representing NM_177372
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGCTGGACGAGCTGGACCTGCTGCTGCTGGAAGAGGACGGCGGAGCGGAGGCCGTGCCGCGGG
TGGAGCTACTTCGGAAGAAGGCGGACGCTTTGTTTCCAGAGACAGTTCTGAGCCGAGGAGTGGATAACCG
GTACCTGGTGCTAGCTGTTGAGACCTCGCAGAATGAAAGAGGAGCCGAGGAGAAGCGCTTGCACGTCACG
GCGTCCCAGGACCGAGAGCACGAAGTTCTGTGCATCCTGAGGAATGGCTGGAGCTCAGTACCAGTTGAGC
CAGGGGACATCGTTCACTTAGAGGGAGACTGCACATCTGAGCCGTGGATAATAGACGATGACTTTGGATA
TTTTATTCTGTACCCGGACATGATGATCTCCGGCACAAGCGTCGCTAGCAGCATTCGGTGTCTGAGAAGA
GCTGTCCTGAGCGAGACCTTCAGGGGCTCTGATCCAGCCACTCGCCAGATGCTGATCGGTACAATTCTCC
ACGAAGTCTTTCAGAAAGCGATAAGTGAGAGCTTTGCTCCAGAAAGGCTACAAGAGCTTGCTCTTCAGAC
CCTCCGAGAAGTGAGGCATTTGAAGGAAATGTATCGCTTGAATCTGAGCCAAGATGAAATACTCTGTGAA
GTTGAGGAGTATCTCCCTTCTTTCTCTAAGTGGGCGGAGGACTTCATGCGTAAGGGCCCTTCGTCTGAAT
TCCCTCAAATGCAGCTCTCTCTGCCAAGCGATGGCAGTAACAGAAGTTCACCTTGTAACATTGAAGTAGT
CAAGTCACTGGATATCGAAGAAAGCATCTGGTCCCCTAGGTTTGGACTGAAGGGCAAAATAGACGTCACA
GTCGGGGTCAAAATACATCGAGATTGTAAAATGAAATACAAGGTAATGCCACTGGAGCTGAAGACCGGCA
AAGAGTCCAACTCCATCGAGCACCGGAGTCAGGTGGTCCTGTACACGCTGCTAAGCCAGGAGAGGCGAGA
GGACCCCGAGGCTGGCTGGCTTCTCTACCTCAAGACCGGGCAGATGTACCCTGTGCCCGCCAACCATCTG
GACAAAAGAGAACTGCTGAAGCTCAGGAACTGGCTGGCCGCCTCGCTGCTGCACCGTGTGAGCCGAGCAG
CTCCGGGGGAGGAAGCTCGGCTCTCTGCCTTGCCACAGATAATCGAGGAAGAGAAAACTTGTAAATACTG
TTCGCAGATAGGCAACTGTGCTCTTTATAGCAGAGCAGTTGAAGAGCAGGGAGATGACGCATCCATCCCA
GAAGCCATGCTGTCCAAAATCCAGGAAGAAACACGGCACCTGCAGCTGGCGCACTTGAAGTATTTCAGCC
TGTGGTGTCTCATGTTAACGCTGGAGTCGCAATCTAAAGACAACCGGAAGACTCACCAGAGTATCTGGTT
AACGCCTGCCTCTGAACTGGAGGAGAGTGGCAACTGTGTTGGAAACCTGGTCAGGACGGAGCCTGTATCA
AGAGTTTGTGATGGACAGTATTTACATAATTTTCAGCGGAAAAATGGTCCCATGCCAGCCACCAATCTGA
TGGCAGGTGACAGGATTATTTTAAGTGGAGAAGAGAGAAAACTATTTGCTTTGTCCAAAGGCTACGTGAA
GAAGATGAACAAGGCAGCCGTTACCTGCTTGCTAGACAGGAACTTGTCGACACTCCCAGCAACAACGGTG
TTTCGATTAGACCGGGAAGAAAGACATGGCGACATAAGTACTCCACTAGGAAATCTGTCTAAATTGATGG
AAAGCACAGATCCCAGCAAAAGACTTCGAGAATTAATCATCGATTTCAGGGAACCTCAGTTCATCGCCTA
CCTCAGCTCCGTCCTTCCACACGATGCGAAGGATACGGTGGCCAACATTCTAAAGGGTTTGAATAAACCC
CAGAGACAAGCAATGAAGAGAGTCCTTCTTTCGAAAGACTACACGCTCATTGTTGGGATGCCAGGGACAG
GAAAGACAACCACCATCTGTGCCCTGGTGAGGATCCTCTCTGCCTGTGGGTTCAGCGTTCTGCTGACCAG
CTACACGCACTCCGCCGTTGACAATATTCTCCTGAAGTTAGCCAAGTTTAAAGTCGGATTTTTGCGCTTG
GGTCAGTCTCATAAGGTCCATCCAGACATCCAGAAGTTTACAGAGGAGGAGATTTGCAGATCGAGGTCCA
TTGCGTCTTTAGCCCATCTAGAAGAACTCTATAACAGTCACCCTATTGTTGCAACAACCTGCATGGGAAT
AAATCACCCAATATTTTCCCGAAAAACCTTTGATTTCTGTATTGTGGATGAAGCATCTCAAATCAGCCAG
CCCGTCTGCCTGGGCCCACTGTTCTTTTCGCGGAGGTTTGTGCTGGTGGGGGACCATCAGCAGCTGCCTC
CCCTGGTTGTAAACCGAGAGGCAAGAGCTCTGGGCATGAGCGAGAGCTTGTTCAAGAGGCTGGAGCGGAA
CGAGAGCGCTGTGGTGCAGCTGACCGTGCAATACCGAATGAACAGGAAGATTATGTCCCTGAGCAACAAG
CTCACATACGCAGGGAAACTGGAGTGTGGGTCAGACCGAGTGGCCAACGCAGTGCTAGCCCTGCCCAACC
TCAAGGACGCAAGGCTGAGCCTCCAGCTTTATGCCGACTACTCTGACAGCCCCTGGCTGGCTGGAGTGTT
AGAGCCAGACAATCCCGTGTGTTTCCTTAACACAGACAAGGTCCCAGCTCCAGAGCAAGTTGAAAATGGT
GGTGTTAGCAATGTCACAGAAGCCAGACTCATTGTCTTCCTAACCTCAACTTTTATAAAGGCAGGCTGCA
GCCCCTCAGACATTGGCGTCATCGCCCCGTACCGACAGCAGCTGCGGATCATCAGCGACTTACTGGCCCG
GTCTTCTGTTGGGATGGTTGAGGTTAACACAGTAGACAAATACCAAGGAAGGGACAAGAGTCTCATCCTG
GTGTCCTTTGTTAGGAGTAATGAGGATGGAACTCTCGGCGAGCTCCTGAAAGATTGGCGGAGACTCAACG
TGGCACTCACCAGAGCCAAGCATAAACTGATCCTGCTGGGCAGCGTGTCCTCGCTGAAGCGCTTCCCGCC
TCTGGGGACGCTCTTTGACCATCTGAATGCAGAGCAGTTAATCCTGGACCTTCCTTCCAGAGAGCATGAG
AGCCTCAGCCACATCCTGGGCGATTGTCAGCGAGACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177372
Insert Size 3189 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177372.3, NP_796346.2
RefSeq Size 4122 bp
RefSeq ORF 3189 bp
Locus ID 327762
UniProt ID Q6ZQJ5
Gene Summary Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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