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Uba1 (NM_009457) Mouse Untagged Clone
CAT＃: MC223393
Uba1 (untagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, (10ug)
CNY 13,590.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A1; A1S9; Sb; Sbx; Ube-1; Ube1x |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223393 representing NM_009457
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTCTATTCAGGCGTCTATGGTATACCCCAGACCCGGCCCTCTAAGGCCCCCGCGCTTCCGAGCT CCGCGCAAACTCTGGTTCTTTTTGCACGATCGAGGTGGTTTGCTCTTCCGTTGCCCTGTGGCTTCGGCTT GTCTCCAGAAGGAAGGCGAGGCTTCCGCCCAGCACTGGGGATGTCCAGCTCGCCGCTGTCCAAGAAACGT CGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCACAGTCTGCGCTGTCCGAAGTGT CCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGAAGCAGACATAGACGAGAGCCTTTACTCCCG GCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTCCAGACATCCAGCGTCCTTGTCTCAGGCTTG CGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTCACCCTACATGACC AAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTACCTTCGGGAGGAGGACATTGGTAAAAATCG AGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGCTATGTACCTGTCACTGCCTACACTGGGCCT CTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCCTCACCAACAGCCCCCTGGAAGCCCAGCTGC GAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGTGGTGGCAGATACAAGAGGCCTGTTTGGGCA ACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATG GTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCTGCCTTGATGAGGCCCGACATGGCTTTGAGA CTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGATCCAACTCAATGGATGTCAGCCCATGGAGAT CAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACTTCCAACTTCTCTGACTACATCCGTGGAGGC ATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTAAATCCTTGCCAGCATCACTGGTAGAGCCTG ACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGCCCAACTGCACATTGGCTTCCAAGCTCTGCA CCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGAAATGAGGAAGATGCAACAGAGCTGGTGGGC CTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAAAACAGAACAGCTTGGATGAAGACCTTATTC GGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCATAAATGCTTTCATTGGGGGCCTTGCTGCCCA GGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATCATGCAGTGGTTGTACTTTGATGCTCTTGAA TGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGTGCCTCCCACGTCAGAACCGTTACGATGGGC AGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAGCAAGCAAAAGTACTTCCTGGTGGGTGCAGG GGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATTGGGCTGGGTTGTGGAGAGGGTGGAGAGGTC GTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGAACCGACAGTTTCTCTTCCGGCCCTGGGATG TCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCGCCAGATGAATCCTTACATCCAGGTGACAAG CCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTATGATGATGATTTCTTCCAAAATTTGGATGGT GTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACATGGATCGCCGATGTGTGTACTATCGGAAGC CACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGTGCAGGTGGTAATCCCCTTCCTGACAGAATC CTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCCATTTGTACCCTGAAAAACTTTCCCAATGCC ATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAGGCCTTTTCAAGCAGCCAGCAGAAAATGTTA ATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATTGCGGCTGGCTGGTACCCAGCCATTGGAGGT GCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCACAGACTTGGGGAGACTGTGTGACCTGGGCC TGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGCAACTGCTGCACAACTTTCCTCCTGACCAGC TCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACGCTGTCCACACCCACTTACTTTTGATGTTAA CAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAACCTTTTTGCCCAGACCTATGGGTTGACTGGA TCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAGTACAAGTCCCAGAGTTCACCCCCAAGTCTG GTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGCCAATGCCTCTGTTGATGACAGCCGTCTTGA GGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCCGGATTTAAGATGTACCCCATTGATTTTGAG AAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGGCTGCATCCAATCTTCGGGCCGAAAACTATG ATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACAAC CACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTACAAGGTAGTTCAAGGGCACCAACAGCTCGAT TCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCTTTGGGTTTTCTGAACCTCTTGCTGCACCTC GTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCGCTTTGAAGTACAAGGGCTGCAGCCTAATGG TGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAGACAGAGCACAAATTGGAGATCACCATGCTG TCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAGCTGCTAAGCTCAAGGAACGATTGGATCAGC CGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCTGGGCCGCCATGTGCGGGCACTGGTGCTTGA GCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTCCCTTATGTCCGATATACCATTCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009457 |
| Insert Size | 3357 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009457.4, NP_033483.2 |
| RefSeq Size | 4068 bp |
| RefSeq ORF | 3357 bp |
| Locus ID | 22201 |
| UniProt ID | Q02053 |
| Gene Summary | This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) uses an alternate 5' exon structure and an upstream start codon, compared to variant 3. The encoded isoform (1) has a longer N-terminus, compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211556 | Uba1 (tGFP-tagged) - Mouse ubiquitin-activating enzyme E1, Chr X (Ube1x) |
CNY 9,220.00 |
|
| MG219278 | Uba1 (tGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1) transcript variant 1, (10ug) |
CNY 11,590.00 |
|
| MR219278 | Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 7,376.00 |
|
| MR219278L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 12,450.00 |
|
| MR219278L4 | Lenti ORF clone of Uba1 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 12,450.00 |
