Tlr8 (NM_133212) Mouse Untagged Clone

CAT#: MC223290

Tlr8 (untagged) - Mouse toll-like receptor 8 (Tlr8), (10ug)



  "NM_133212" in other vectors (4)

CNY 13,300.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223290 representing NM_133212
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAACATGCCCCCTCAGTCATGGATTCTGACGTGCTTTTGTCTGCTGTCCTCTGGAACCAGTGCCA
TCTTCCATAAAGCGAACTATTCCAGAAGCTATCCTTGTGACGAGATAAGGCACAACTCCCTTGTGATTGC
AGAATGCAACCATCGTCAACTGCATGAAGTTCCCCAAACTATAGGCAAGTATGTGACAAACATAGACTTG
TCAGACAATGCCATTACACATATAACGAAAGAGTCCTTTCAAAAGCTGCAAAACCTCACTAAAATCGATC
TGAACCACAATGCCAAACAACAGCACCCAAATGAAAATAAAAATGGTATGAATATTACAGAAGGGGCACT
TCTCAGCCTAAGAAATCTAACAGTTTTACTGCTGGAAGACAACCAGTTATATACTATACCTGCTGGGTTG
CCTGAGTCTTTGAAAGAACTTAGCCTAATTCAAAACAATATATTTCAGGTAACTAAAAACAACACTTTTG
GGCTTAGGAACTTGGAAAGACTCTATTTGGGCTGGAACTGCTATTTTAAATGTAATCAAACCTTTAAGGT
AGAAGATGGGGCATTTAAAAATCTTATACACTTGAAGGTACTCTCATTATCTTTCAATAACCTTTTCTAT
GTGCCCCCCAAACTACCAAGTTCTCTAAGGAAACTTTTTCTGAGTAATGCCAAAATCATGAACATCACTC
AGGAAGACTTCAAAGGACTGGAAAATTTAACATTACTAGATCTGAGTGGAAACTGTCCAAGGTGTTACAA
TGCTCCATTTCCTTGCACACCTTGCAAGGAAAACTCATCCATCCACATACATCCTCTGGCTTTTCAAAGT
CTCACCCAACTTCTCTATCTAAACCTTTCCAGCACTTCCCTCAGGACGATTCCTTCTACCTGGTTTGAAA
ATCTGTCAAATCTGAAGGAACTCCATCTTGAATTCAACTATTTAGTTCAAGAAATTGCCTCGGGGGCATT
TTTAACAAAACTACCCAGTTTACAAATCCTTGATTTGTCCTTCAACTTTCAATATAAGGAATATTTACAA
TTTATTAATATTTCCTCAAATTTCTCTAAGCTTCGTTCTCTCAAGAAGTTGCACTTAAGAGGCTATGTGT
TCCGAGAACTTAAAAAGAAGCATTTCGAGCATCTCCAGAGTCTTCCAAACTTGGCAACCATCAACTTGGG
CATTAACTTTATTGAGAAAATTGATTTCAAAGCTTTCCAGAATTTTTCCAAACTCGACGTTATCTATTTA
TCAGGAAATCGCATAGCATCTGTATTAGATGGTACAGATTATTCCTCTTGGCGAAATCGTCTTCGGAAAC
CTCTCTCAACAGACGATGATGAGTTTGATCCACACGTGAATTTTTACCATAGCACCAAACCTTTAATAAA
GCCACAGTGTACTGCTTATGGCAAGGCCTTGGATTTAAGTTTGAACAATATTTTCATTATTGGGAAAAGC
CAATTTGAAGGTTTTCAGGATATCGCCTGCTTAAATCTGTCCTTCAATGCCAATACTCAAGTGTTTAATG
GCACAGAATTCTCCTCCATGCCCCACATTAAATATTTGGATTTAACCAACAACAGACTAGACTTTGATGA
TAACAATGCTTTCAGTGATCTTCACGATCTAGAAGTGCTGGACCTGAGCCACAATGCACACTATTTCAGT
ATAGCAGGGGTAACGCACCGTCTAGGATTTATCCAGAACTTAATAAACCTCAGGGTGTTAAACCTGAGCC
ACAATGGCATTTACACCCTCACAGAGGAAAGTGAGCTGAAAAGCATCTCACTGAAAGAATTGGTTTTCAG
TGGAAATCGTCTTGACCGTTTGTGGAATGCAAATGATGGCAAATACTGGTCCATTTTTAAAAGTCTCCAG
AATTTGATACGCCTGGACTTATCATACAATAACCTTCAACAAATCCCAAATGGAGCATTCCTCAATTTGC
CTCAGAGCCTCCAAGAGTTACTTATCAGTGGTAACAAATTACGTTTCTTTAATTGGACATTACTCCAGTA
TTTTCCTCACCTTCACTTGCTGGATTTATCGAGAAATGAGCTGTATTTTCTACCCAATTGCCTATCTAAG
TTTGCACATTCCCTGGAGACACTGCTACTGAGCCATAATCATTTCTCTCACCTACCCTCTGGCTTCCTCT
CCGAAGCCAGGAATCTGGTGCACCTGGATCTAAGTTTCAACACAATAAAGATGATCAATAAATCCTCCCT
GCAAACCAAGATGAAAACGAACTTGTCTATTCTGGAGCTACATGGGAACTATTTTGACTGCACGTGTGAC
ATAAGTGATTTTCGAAGCTGGCTAGATGAAAATCTGAATATCACAATTCCTAAATTGGTAAATGTTATAT
GTTCCAATCCTGGGGATCAAAAATCAAAGAGTATCATGAGCCTAGATCTCACGACTTGTGTATCGGATAC
CACTGCAGCTGTCCTGTTTTTCCTCACATTCCTTACCACCTCCATGGTTATGTTGGCTGCTCTGGTTCAC
CACCTGTTTTACTGGGATGTTTGGTTTATCTATCACATGTGCTCTGCTAAGTTAAAAGGCTACAGGACTT
CATCCACATCCCAAACTTTCTATGATGCTTATATTTCTTATGACACCAAAGATGCATCTGTTACTGACTG
GGTAATCAATGAACTGCGCTACCACCTTGAAGAGAGTGAAGACAAAAGTGTCCTCCTTTGTTTAGAGGAG
AGGGATTGGGATCCAGGATTACCCATCATTGATAACCTCATGCAGAGCATAAACCAGAGCAAGAAAACAA
TCTTTGTTTTAACCAAGAAATATGCCAAGAGCTGGAACTTTAAAACAGCTTTCTACTTGGCCTTGCAGAG
GCTAATGGATGAGAACATGGATGTGATTATTTTCATCCTCCTGGAACCAGTGTTACAGTACTCACAGTAC
CTGAGGCTTCGGCAGAGGATCTGTAAGAGCTCCATCCTCCAGTGGCCCAACAATCCCAAAGCAGAAAACT
TGTTTTGGCAAAGTCTGAAAAATGTGGTCTTGACTGAAAATGATTCACGGTATGACGATTTGTACATTGA
TTCCATTAGGCAATACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133212
Insert Size 3099 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133212.3, NP_573475.2
RefSeq Size 5047 bp
RefSeq ORF 3099 bp
Locus ID 170744
UniProt ID P58682
Gene Summary Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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