Nlrc4 (NM_001033367) Mouse Untagged Clone

CAT#: MC223257

Nlrc4 (untagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4), (10ug)



  "NM_001033367" in other vectors (4)

CNY 13,210.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9530011P19Rik; Card12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; IPAF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223257 representing NM_001033367
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTTATAAGGAACAACAGACGAGCCCTTATTCAAAGGATGGGCTTAACAGTTACCAAGCAAATCT
GCGATGACCTCTTTGCATTGAACGTTCTCAACAATCAAGAAGCTAATGTCATTTACTGTGAGCCCTTGGA
GCAGGAAGCCGCCCGAAAGATCATCCATATGACTATGCAGAAGGGCTCAGCGGCCTGCAACCTCTTTCTT
AAGAGTCTTGAAAACTGGGACTATTTTGTGTATCAGGACTTAACTGGACAAAATCTTTCTTATCAGGTCA
CAGAAGAAGACCTGAATGTTTTGGCCCAGAATTTAAAGGACTTGTACAACAGCCCTGCTTTTCTGAACTT
CTACCCCCTGGGTGAAGATATCGACATAATTTTTAATCTGGAGAAAACCTTCACAGAACCTATCATGTGG
AAGAAGGACCATCGTCATCACCGTGTGGAGCAGCTGACTTTGGGCAGCCTGCTCGAGGCTCTGAAGAGCC
CCTGCCTGATTGAAGGCGAGTCTGGCAAAGGGAAGTCCACCCTGCTGCAGAGAATCGCTATGCTCTGGGC
CTCTGGGGGCTGCAGGGCTCTGAAGGGGTTCAGATTAGTCTTCTTCATCCACCTGAGAAGCGCCAGGGGG
GGACTATTCGAAACACTGTACGATCAGCTCCTGAACATACCCGACTTCATCAGCAAGCCGACCTTCAAGG
CTCTGCTGCTGAAGCTACACAAGGAGGTCCTCTTTCTTCTCGATGGTTACAATGAATTCCATCCCCAGAA
CTGCCCAGAAATTGAAGCCCTGATAAAGGAAAACCATCGCTTCAAGAACATGGTCATTGTCACCACCACC
ACGGAGTGCCTGAGGCATATCAGACATGTTGGCGCCCTGACTGCGGAGGTGGGAGATATGACCGAAGACA
GTGCCAAAGATCTCATCGAGGCAGTGCTGGTACCTGATCAGGTTGAACGCCTGTGGGCCCAAATCCAGGA
GTCCAGGTGCCTGAGAAATCTGATGAAGACCCCTCTCTTCGTGGTGATCACCTGTGCAATTCAGATGGGC
AGACAGGAATTCCAAGCTCACACCCAAACCATGCTGTTCCAAACCTTCTACGACCTCCTGATACAGAAAA
ACAGCCACAGATATAGAGGTGGAGCTTCAGGTGATTTTGCCAGGAGCCTAGACTACTGTGGAGACCTGGC
CCTAGAAGGTGTGTTCGCCCACAAATTTGATTTTGAACCCGAGCATGGGTCCAGCATGAACGAGGACGTC
CTGGTGACAATAGGGCTCCTCTGTAAGTACACAGCTCAGAGGCTGAAGCCCACGTATAAATTCTTTCATA
AATCATTTCAGGAGTACACGGCAGGTCGGAGACTCAGCAGTTTGCTGACGTCCAAAGAGCCAGAGGAGGT
GAGCAAAGGGAACAGCTACTTAAACAAAATGGTTTCCATCTCTGACATCACATCCCTATATGGCAATCTG
CTCCTCTACACGTGTGGGTCGTCCACAGAAGCAACCAGGGCGGTCATGAGGCACCTTGCAATGGTTTATC
AGCACGGCAGCCTACAAGGACTTTCAGTCACCAAGAGGCCTCTCTGGAGGCAGGAATCAATCCAGAGTCT
GAGAAATACCACTGAGCAAGATGTTCTGAAAGCCATCAATGTAAATTCCTTCGTAGAGTGTGGCATCAAT
TTGTTCTCAGAGAGTATGTCTAAATCAGACCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGTT
TATACATCAACTCAGAGAACATCCCTGACTATTTATTTGACTTCTTTGAATACTTGCCTAATTGTGCAAG
CGCATTGGACTTCGTGAAGTTGGATTTCTATGAAAGAGCTACAGAGTCACAGGACAAGGCAGAAGAGAAT
GTCCCTGGAGTTCACACAGAAGGGCCCTCAGAAACCTACATTCCCCCCAGGGCTGTGTCTTTGTTCTTCA
ACTGGAAGCAGGAATTCAAGACTCTAGAGGTCACACTCCGAGATATTAACAAGTTGAATAAGCAAGATAT
CAAATATCTGGGGAAGATATTCAGCTCTGCCACCAACCTCCGGCTGCATATCAAGAGATGTGCAGCCATG
GCTGGAAGACTCAGCTCAGTCCTCAGAACCTGCAAGAACATGCATACCCTCATGGTGGAAGCCAGTCCCC
TCACCACGGATGACGAACAGTACATCACATCTGTGACAGGCCTCCAGAACTTAAGTATTCACCGCTTGCA
CACTCAACAGCTGCCAGGTGGTCTGATTGACAGCTTGGGTAATCTGAAGAACCTCGAGAGACTCATACTG
GATGACATCAGGATGAACGAGGAAGATGCTAAAAACCTAGCCGAAGGCCTACGAAGCCTGAAGAAGATGC
GTTTACTCCATTTGACTCATTTGTCTGACATTGGGGAGGGGATGGACTACATAGTCAAGTCTCTCTCAGA
AGAATCCTGTGATCTCCAAGAGATGAAGTTGGTGGCCTGCTGTCTGACTGCAAACTCTGTGAAAGTTCTA
GCACAGAATCTTCACAATTTGATCAAGCTGAGCATTCTTGATATATCAGAAAATTACCTGGAAAAGGATG
GGAATGAAGCTCTACAGGAACTGATCGGCAGGCTTGGCGTTCTGGGAGAGCTCACTACATTGATGCTGCC
TTGGTGCTGGGATGTGCACACCAGCCTGCCCAAGCTGTTGAAGCAGTTGGAGGGGACCCCAGGACTTGCC
AAACTTGGATTGAAAAACTGGAGACTCAGAGACGAAGAGATTAAAAGTTTAGGTGAATTTCTGGAGATGA
ATCCTCTGAGAGACTTGCAGCAGTTGGATTTAGCGGGGCACTGTGTGAGCAGTGACGGATGGCTTTACTT
CATGAATGTGTTTGAGAATCTGAAGCAGTTAGTGTTTTTTGACTTTAGCACTGAGGAGTTCTTACCGGAT
GCAGCACTGGTGAGGAAACTTAGTCAAGTGTTATCCAAGTTAACTCTTCTGCAAGAGGTAAAGCTCACGG
GCTGGGAGTTTGATGACTATGATATTAGCGCTATTAAAGGCACCTTTAAACTAGTGACTGCTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001033367
Insert Size 3075 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001033367.3, NP_001028539.1
RefSeq Size 3838 bp
RefSeq ORF 3075 bp
Locus ID 268973
UniProt ID Q3UP24
Gene Summary Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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