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Kl (NM_013823) Mouse Untagged Clone
CAT＃: MC223205
Kl (untagged) - Mouse klotho (Kl), (10ug)
CNY 10,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | alpha-kl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223205 representing NM_013823
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCCCGCGCCCCTCCTCGCCGCCCGCCGCGGCTGGTGCTGCTCCGTTTGCTGTTGCTGCATCTGC TGCTGCTCGCCCTGCGCGCCCGCTGCCTGAGCGCTGAGCCGGGTCAGGGCGCGCAGACCTGGGCTCGCTT CGCGCGCGCTCCTGCCCCAGAGGCCGCTGGCCTCCTCCACGACACCTTCCCCGACGGTTTCCTCTGGGCG GTAGGCAGCGCCGCCTATCAGACCGAGGGCGGCTGGCGACAGCACGGCAAAGGCGCGTCCATCTGGGACA CTTTCACCCATCACTCTGGGGCGGCCCCGTCCGACTCCCCGATCGTCGTGGCGCCGTCGGGTGCCCCGTC GCCTCCCCTGTCCTCCACTGGAGATGTGGCCAGCGATAGTTACAACAACGTCTACCGCGACACAGAGGGG CTGCGCGAACTGGGGGTCACCCACTACCGCTTCTCCATATCGTGGGCGCGGGTGCTCCCCAATGGCACCG CGGGCACTCCCAACCGCGAGGGGCTGCGCTACTACCGGCGGCTGCTGGAGCGGCTGCGGGAGCTGGGCGT GCAGCCGGTGGTTACCCTGTACCATTGGGACCTGCCACAGCGCCTGCAGGACACCTATGGCGGATGGGCC AATCGCGCCCTGGCCGACCATTTCAGGGATTATGCCGAGCTCTGCTTCCGCCACTTCGGTGGTCAGGTCA AGTACTGGATCACCATTGACAACCCCTACGTGGTGGCCTGGCACGGGTATGCCACCGGGCGCCTGGCCCC GGGCGTGAGGGGCAGCTCCAGGCTCGGGTACCTGGTTGCCCACAACCTACTTTTGGCTCATGCCAAAGTC TGGCATCTCTACAACACCTCTTTCCGCCCCACACAGGGAGGCCGGGTGTCTATCGCCTTAAGCTCCCATT GGATCAATCCTCGAAGAATGACTGACTATAATATCAGAGAATGCCAGAAGTCTCTTGACTTTGTGCTAGG CTGGTTTGCCAAACCCATATTTATTGATGGCGACTACCCAGAGAGTATGAAGAACAACCTCTCGTCTCTT CTGCCTGATTTTACTGAATCTGAGAAGAGGCTCATCAGAGGAACTGCTGACTTTTTTGCTCTCTCCTTCG GACCAACCTTGAGCTTTCAGCTATTGGACCCTAACATGAAGTTCCGCCAATTGGAGTCTCCCAACCTGAG GCAGCTTCTGTCTTGGATAGATCTGGAATATAACCACCCTCCAATATTTATTGTGGAAAATGGCTGGTTT GTCTCGGGAACCACCAAAAGGGATGATGCCAAATATATGTATTATCTCAAGAAGTTCATAATGGAAACCT TAAAAGCAATCAGACTGGATGGGGTCGACGTCATTGGGTACACCGCGTGGTCGCTCATGGACGGTTTCGA GTGGCATAGGGGCTACAGCATCCGGCGAGGACTCTTCTACGTTGACTTTCTGAGTCAGGACAAGGAGCTG TTGCCAAAGTCTTCGGCCTTGTTCTACCAAAAGCTGATAGAGGACAATGGCTTTCCTCCTTTACCTGAAA ACCAGCCCCTTGAAGGGACATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTACGTTCAAGTGGA CACTACTCTCTCTCAGTTTACTGACCCGAATGTCTATCTGTGGGATGTGCATCACAGTAAGAGGCTTATT AAAGTAGACGGGGTTGTAGCCAAGAAGAGAAAACCTTACTGTGTTGATTTCTCTGCCATCCGGCCTCAGA TAACCTTACTTCGAGAAATGCGGGTCACCCACTTTCGCTTCTCCCTGGACTGGGCCCTGATCTTGCCTCT GGGTAACCAGACCCAAGTGAACCACACGGTTCTGCACTTCTACCGCTGCATGATCAGCGAGCTGGTGCAC GCCAACATCACTCCAGTGGTGGCCCTGTGGCAGCCAGCAGCCCCGCACCAAGGCCTGCCACATGCCCTTG CAAAACATGGGGCCTGGGAGAACCCGCACACTGCTCTGGCGTTTGCAGACTACGCAAACCTGTGTTTTAA AGAGTTGGGTCACTGGGTCAATCTCTGGATCACCATGAACGAGCCAAACACACGGAACATGACCTATCGT GCCGGGCACCACCTCCTGAGAGCCCATGCCTTGGCTTGGCATCTGTACGATGACAAGTTTAGGGCGGCTC AGAAAGGCAAAATATCCATCGCCTTGCAGGCTGACTGGATAGAACCGGCCTGCCCTTTCTCTCAAAATGA CAAAGAAGTGGCCGAGAGAGTTTTGGAATTTGATATAGGCTGGCTGGCAGAGCCTATTTTTGGTTCCGGA GATTATCCACGTGTGATGAGGGACTGGCTGAACCAAAAAAACAATTTTCTTTTGCCCTATTTCACCGAAG ATGAAAAAAAGCTAGTCCGGGGTTCCTTTGACTTCCTGGCGGTGAGTCATTACACCACCATTCTGGTAGA CTGGGAAAAGGAGGATCCGATGAAATACAACGATTACTTGGAGGTACAGGAGATGACTGACATCACATGG CTCAACTCTCCCAGTCAGGTGGCAGTGGTGCCTTGGGGGCTGCGCAAAGTGCTCAACTGGCTAAGGTTCA AGTACGGAGACCTCCCGATGTATGTGACAGCCAATGGAATCGATGATGACCCCCACGCCGAGCAAGACTC ACTGAGGATCTATTATATTAAGAATTATGTGAATGAGGCTCTGAAAGCCTACGTGTTGGACGACATCAAC CTTTGTGGCTACTTTGCGTATTCACTTAGTGATCGCTCAGCTCCCAAGTCTGGCTTTTATCGATATGCTG CGAATCAGTTTGAGCCCAAACCATCTATGAAACATTACAGGAAAATTATTGACAGCAATGGCTTCCTGGG TTCTGGAACACTGGGAAGGTTTTGTCCAGAAGAATACACTGTGTGCACCGAATGTGGATTTTTTCAAACC CGGAAGTCTTTGCTGGTCTTCATCTCGTTTCTTGTTTTTACTTTTATTATTTCTCTTGCTCTCATTTTTC ACTACTCCAAGAAAGGCCAGAGAAGTTATAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013823 |
| Insert Size | 3045 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC138258, AAI38259 |
| RefSeq Size | 5124 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 16591 |
| UniProt ID | O35082 |
| Gene Summary | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227101 | Kl (tGFP-tagged) - Mouse klotho (Kl), (10ug) |
CNY 12,200.00 |
|
| MR227101 | Kl (Myc-DDK-tagged) - Mouse klotho (Kl) |
CNY 10,600.00 |
|
| MR227101L1 | Lenti ORF clone of Kl (Myc-DDK-tagged) - Mouse klotho (Kl) |
CNY 13,000.00 |
|
| MR227101L2 | Lenti ORF clone of Kl (mGFP-tagged) - Mouse klotho (Kl) |
CNY 13,000.00 |
|
| MR227101L3 | Lenti ORF clone of Kl (Myc-DDK-tagged) - Mouse klotho (Kl) |
CNY 13,000.00 |
|
| MR227101L4 | Lenti ORF clone of Kl (mGFP-tagged) - Mouse klotho (Kl) |
CNY 12,070.00 |
