Evc (NM_021292) Mouse Untagged Clone

CAT#: MC223178

Evc (untagged) - Mouse Ellis van Creveld gene homolog (human) (Evc), (10ug)



  "NM_021292" in other vectors (4)

CNY 13,020.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223178 representing NM_021292
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTGCACTAAAGATGCGCGGCTGCAGCTGGGCCGGGAAGCCCTGCAGGCGGCGCCGACGCTGCTGG
TCCCCGCGGTGCTACTGGGTGGCGTTCTCGGCCTAGGCCTTGGCTTGTGGCTTGGCTGTCGCGCGAGCCA
CTTGCGCGCACGGCTCCAGAAAGATGACAGGAAACGTCTGCTCGGGAGTTCAGAGCCCCCTGCACAGAGC
CTACGGGACACTGGCTCTCAAGCCAAAGCCAGGAGGCGCCAGAGAGAAACCACTCGGGATGAAGACGCCC
CTGAGGTGTGTGAGCCATCCTTGAGTGGCAACATCACGGCATTTGCACTGAAAGCCAGAGTTGTCTATCC
CATCAACCAGAAGTTCCGGCCTCTGGCCGACGGATCCTCCCACCCATCGCTGCATGAAAACCTGACACAG
GCAGCGGCCATCCTGCCCCACCTGCCCCACCAGCCAGCAGAGGCCTCACCAGCCAGCAGCCTGGGCAGCC
TGAGCCAGGCTGGCAAGGAGGACGGCAGCTCCTCCTCCAGCATGCGGTCAACCTACAGCGACGACCGGAT
CCTCCAGTGTGCCTTTCTCCGGGTGGGCAGCTTCCCTGAGATCCTGGCCTGTGAGAGTGTGGACATTGAC
CTGTGTGTCTGCAGCCTTCACCTGAAAGACCTGCTTCAGGTAGACACGGCGCTGAGGCAAGAGAAGCACC
TGATGTTCATTCAGATTCTTAAAGCATGCCTCCTGGATTTTTTTCCTAAAAAGAAGCCTGATGATGAATT
ATGCCAGAAGGTTCTTTCCAAACAAGAGCATGATTTGGAGGAATTAGAAAAGGGACTCCAGGCCAGATTG
GCGAACACAGAAATGTTAGGCACTGGCGACTCTGGCTACGTTTCCCTGGCAGATGTGGAGAGGAAGGAGA
GAGAGCTCTCAGAGCAGCTCATAGACAACATGGGAGCTTTCTGGAAGCAGATGGAAAGCATCCAGCCCAC
TCTCATGGACCAGTTTAAGTGTTCCAGCTCCAAAGCTCGGCAGTTCATGATGACTCTGACAGGAAGAATG
ATTGTCGCTGAAGGACTGCTGCATGACTCTCAGGACCTGCATGTCCTGGACACCCTGGAGAGGACAATGG
GTCGATCCCACTTGGCAAGAATGGTGGAGTTCCTGAGAACCCAGATCCAGGAGGAGACCAAGTGCAGACT
TGCTGCCATCTCCCGTGGCCTGGAGTTGCTGACTGTCCAGGGGCAGCTGTCTGGGAGGCAAAAGGAGGAA
CTCCTGACTCAGCAGCACAAGGCCTTCTGGGAGGAGGCAGAGCGTTTTGGCAGGGAATTCACGCAGCGGG
GCAAAGATCTGGTCCAGGCGTCTCAGGCTCGCCAGGCAGAGGCAGCAGCAGAGCTCACACAGACCCAAGA
AGAAGAACGGAGGAGTTTCCTGGCTGATTCCCAGCTGACCTCGGATCCTGGGGAATTCCTCAAGGCTTTT
CATGAGGTCCTGGAGAGGCAGCGGCTGACAAGGAGTGACCAAGAGGGAGACGAGGACACCCGAATCACTG
AGGCCATGGCTGCGCTCTGCCAGGAGCTGTACTGTAGCACCATGGGAACCTTCCAAAAGTTTGTGGACTC
CCTGTTCCTGAAGACCCTCCCAGAAGTGACCAGCCTCCCTGTGGCAGAATGTGAGACCCTGCGGCAGCAG
GTCCAGGAGCAGGCAGCAAGGCAGCTGGGACAGGCAGACCGATTCCGCAGACGACAGTGGGGACTCCTAT
GTGATCTATTGGAGCAGGACAAACGGGTGTGGCTGGAGGAGGGTACGCTGTCCACGGTGCTGCAGAGGCA
GCTGCGGGATCACCACGAGAGCACCATCCACGGCGTCCTGAGCAGATTCAGTGGCCTTTCTGAAGAGTCC
TCACGAGGCATCCTGCAGGGCCACGAACTGCTGCTGTGCTCTGCTCTGCGCAGACTGGCCCTCCGAGGCA
CCACCATCACTGCCCTGGCTCAGATGAGGCTGTCTGGGAAGAAGAGGCTCCTGCAGGAGCTACATGAGCA
GCTGGCATTAGAGCAGGGTGTGTCCCCATGCCTGGAGGAGCATCAATGGCAGCTGCTTAGAGCCCTGGAG
GCGCGGATCCAGGAGGAGGCAGCCAGGTTGGAGGATGAGGCACAGCAGACAGGTCTGCGGCTCCAGCAGC
AGCTGCTGGCGGAGGCACAGGAGGCAGGGCGGCTGCTACAGCTGCACATGGAGCGGGTCATTGGGCAGGC
GCTGCTGGTACATGCTAGGAATGTTGCCAGCAAAGGCCGGACCAGGGAGAAGGAAGACTTCAAGAGGACG
CTGGTAGAGACGGTGGTGGAGAGTGTGTATGTGACCAGCACTAGTGTCAACCGCCTCGTGCAGGCGCATT
ACCAGGCCGTCGGGAAGCTCCTGCAGGCTCATGAGGAACAATTGCTGCAGCGCCTAAAGACCCTGCAGGG
AGAGAGAATCAATGCTTACAAGCTGTGGAAGAAGCAAGAATTCAGTGACCCGTCGTTGGAGAGTCAAACA
GCGGATGGTACCCATGGAGCTTCCCAGGGGGTACAGCAGAGGATGCTTTCGCAGCAGAAGAGGCTCCTGG
ACCAGTTCACGAAGCATCAGCAAGGCCGCCTGAACTCTCAGAGGCAGAAGGCACAAGAGCTGGACCAGCT
GCAAGCCCAGCTTGAGACCCAGCTGCAGGAAGCTGAGCAGACCCTTATCTCAGAGCTATCGACATTGGCC
CGAGTACCCCTTCCTGAGAACAAGCCATTCTCCAACAAGCGAGGACTTCCAGAAAAGCCAGTAAGAACCA
AAAGGAAGAAACCCCCACCTCGGGAGAGAGAGGACCTGGGGACACCAAACGATGACCACCTTGCCTTGGC
CGACCACACCACAGGACCACTCAGTACAACCTACTCAGCATCGCCTCCCATCCGTGTCCACAGCGGCGGA
AGGCTGGATCAGCAGGACAGCGAAGCTGGCGATGGAGAAAGCACAAGCAAGATACTGCAGAAAGGAAGCA
ATCTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_021292
Insert Size 3018 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021292.2, NP_067267.2
RefSeq Size 4317 bp
RefSeq ORF 3018 bp
Locus ID 59056
UniProt ID P57680
Gene Summary Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:17660199, PubMed:24582806). Involved in endochondral growth and skeletal development (PubMed:17660199).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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