Mybpc2 (NM_178067) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC28149
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223146 representing NM_178067 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGGCTAAACCAGCGGCCAAAAAGGCCTCCAAAGGCAAAGATGCCCCAAAGGAAGCCCCTGCAA AGCAGACTCCTGAAGAGCCCCCCAAAGAGGCCCCACCTGAGGACCAATCCCCAACTGCGGAGGAGCCCAC AGGCATCTTCCTGAAAAAGCCGGACTCTGTGTCAGTGGAGACTGGGAAAGACGCGGTGATTCTGGCCAAG GTGAACGGGAAGGAGCTCCCAGGCAAGCCGACCATCAAGTGGTTCAAGGGGAAGTGGCAGGAGCTGGGCA GCAAGAGCGGAGCCCGGTTCATCTTCAAGGAATCTCATGACTCTACCAGCAATGTGTACACCGTGGAGCT CCACATTGGGAAGGTGGTCCTAGGGGACCGCGGGGATTACCGTCTAGAGATCAAAGCCAAGGATGTCTGC GACAGCTGCTCGTTCAACGTGGATGTGGAGGCACCCCGTCAGGACTCATCCGGTCAGAGCCTCGAGAGCT TCAAGCGTTCGGGTGACGGGAAGTCAGAAGATGCAGGCGAGCTGGATTTCAGTGGCTTGTTGAAGAAGAG GGAAGTAGTTGAGGAGGAGAAGAAGAAGAAGAAAGATGATGATGATCTGGGTATACCTCCGGAGATTTGG GAGCTCCTGAAAGGGGCCAAGAAGAGCGAGTACGAGAAGATCGCCTTCCAGTACGGCATCACTGACCTCC GTGGCATGCTGAAGCGGCTCAAGAAGGCCAAGGTGGAGGTCAAGAAGAGTGCAGCCTTCACTAAGAAGTT GGACCCAGCCTACCAAGTAGACAGGGGCAACAAGATCAAGCTGGTGGTGGAGATCAGTGACCCAGACCTT CCTCTCAAGTGGTTCAAGAATGGCCAGGAGATCAAACCAAGCAGCAAGTATGTGTTTGAGAACGTTGGGA AGAAACGAATTCTTACCATCAACAAGTGCACGCTGGCGGATGATGCTGCATACGAGGTGGCAGTCCAAGA TGAGAAGTGTTTCACTGAACTCTTTGTCAAAGAGCCCCCAGTCTTGATTGTCACCCCACTGGAGGACCAG CAGGTATTCGTAGGTGACCGAGTGGAAATGTCGGTAGAGGTGTCAGAAGAGGGTGCACAGGTTATGTGGA TGAAAGATGGTGTGGAGATGACTCGGGAGGATTCCTACAAGGCGCGCTACCGCTTCAAGAAGGACGGGAA ACGGCACATCCTCATCTACTCTGATGTGGCCCAGGAGGATGGGGGCCGCTACCAGGTCATAACCAATGGT GGCCAGTGTGAGGCTGAACTCATTGTGGAAGAGAAGCAACTGGAGGTCCTGCAGGACATTGCTGACCTGA CAGTGAAGGCTGCAGAGCAGGCTGTGTTCAAGTGTGAAGTGTCTGATGAGAAGGTGACAGGCAAATGGTA CAAGAACGGCGTGGAGGTACGACCCAGCAAGAGGATCACCATCTCCCATGTGGGCAGATTCCACAAGCTG GTGATTGATGACGTCCGCCCTGAGGATGAGGGAGACTACACATTCGTGCCCGATGGCTATGCCCTATCCC TCTCAGCCAAGCTCAACTTTTTGGAAATCAAAGTGGAGTATGTACCTAAGCAAGAGCCCCCGAAGATCCA CCTGGACTGCTCAGGGAAGACCTCAGATAACTCAATTGTTGTGGTGGCTGGAAACAAGCTGCGGCTGGAT GTGGCCATCACAGGGGAACCACCTCCCACTGCTACCTGGCTAAGGGGAGATGAGGTGTTCACAGCCACAG AAGGCAGGACACACATCGAGCAGAGGCCAGACTGCAGCAGCTTTGTGATCGAGAGTGCGGAGCGGTCGGA TGAGGGCCGCTACACCATCAAAGTCACCAATCCTGTGGGCGAGGACGTGGCCTCCATTTTCCTGCGGGTT GTGGATGTTCCTGATCCTCCAGAGGCTGTCCGTGTCACCTCAGTTGGAGAAAACTGGGCCATTCTGGTCT GGGAGCCACCCAAGTATGATGGAGGACAGCCAGTGACTGGGTACTTGATGGAGCGGAAGAAGAAAGGCTC CCAGCGCTGGATGAAGATCAACTTTGAGGTCTTCACTGACACCACCTACGAGTCCACAAAGATGATCGAG GGCGTCCTTTATGAGATGCGGGTCTTCGCTGTCAACGCCATTGGGGTCTCACAGCCCAGCATGAACACCA AGCCCTTCATGCCCATTGCTCCCACGAGTGCACCACAACACCTCACCGTGGAGGATGTGACGGACACCAC CACCACACTTAAGTGGAGACCTCCAGATCGGATTGGTGCTGGTGGCATTGATGGCTACCTGGTGGAGTAC TGCCTGGAGGGCTCTGAGGAATGGGTACCAGCTAACAAGGAGCCAGTGGAACGCTGTGGCTTCACTGTCA AGGATCTCCCAACTGGAGCCAGGATCCTCTTCCGGGTTGTTGGGGTCAACATCGCAGGGCGCAGTGAACC AGCCACCCTCCTTCAGCCAGTCACTATCAGGGAGATTGTGGAGCAACCTAAGATCCGCCTTCCCCGCCAT CTTCGTCAGACTTATATCCGAAAAGTTGGGGAGGCCCTCAACCTCGTCATCCCCTTCCAGGGCAAGCCCC GGCCTCAGGTGGTGTGGACCAAGGGTGGGGCTCCCTTGGATACCTCCCGTGTGAATGTGCGGACTAGTGA CTTCGACACAGTGTTCTTCGTGCGACAGGCGGCCCGCACTGACTCCGGAGAGTATGAGCTGAGTGTGCAG ATTGAAAACATGAAGGACACTGCTACCATACGCATCCGGGTCGTGGAAAAGGCAGGGCCAGCAGAGAATG TGATGGTGAAGGAGGTGTGGGGCACAAATGCTCTGGTGGAGTGGCAGCCGCCCAAGGATGACGGGAACAG TGAGATCACAGGCTACTTTGTCCAGAAGGCTGACAAAAAGACCATGGAGTGGTTCAATGTCTACGAACAC AATCGCCACACCAGCTGTACGGTGTCTGACCTCATTGTGGGTAATGAGTACTACTTCCGTATCTTCAGCG AGAACATCTGCGGTCTCAGTGACTCGCCAGGTGTCTCCAAGAACACAGCTCGAATTCTCAAGACAGGAAT CACCTTAAAACCACTGGAGTACAAGGAGCATGATTTCCGGACGGCTCCCAAGTTCCTGACCCCGCTGATG GACCGGGTCGTGGTGGCGGGATATACCGCAGCTCTCAACTGTGCCGTCAGAGGCCACCCTAAGCCAAAGG TGGTGTGGATGAAGAACAAGATGGAAATCCATGAAGATCCCAAATTCCTCATAACCAACTACCAGGGCAT CCTGACACTGAACATCCGCCGACCGTCACCCTTTGATGCAGGGACCTATTCCTGCCGCGCCTTCAATGAG CTGGGGGAAGCCCTGGCTGAATGCAAACTGGATGTCCGAGTGCCACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_178067
Insert Size	3411 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178067.3, NP_835168.1
RefSeq Size	3609 bp
RefSeq ORF	3411 bp
Locus ID	233199
Gene Summary	Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role (By similarity).[UniProtKB/Swiss-Prot Function]

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